EA200100287A1 - HIGH-AFFINITY ANTIBODIES - Google Patents
HIGH-AFFINITY ANTIBODIESInfo
- Publication number
- EA200100287A1 EA200100287A1 EA200100287A EA200100287A EA200100287A1 EA 200100287 A1 EA200100287 A1 EA 200100287A1 EA 200100287 A EA200100287 A EA 200100287A EA 200100287 A EA200100287 A EA 200100287A EA 200100287 A1 EA200100287 A1 EA 200100287A1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- samples
- antibody
- incubating
- hour
- sample
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
- C07K16/3007—Carcino-embryonic Antigens
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
- A61K47/6801—Drug-antibody or immunoglobulin conjugates defined by the pharmacologically or therapeutically active agent
- A61K47/6803—Drugs conjugated to an antibody or immunoglobulin, e.g. cisplatin-antibody conjugates
- A61K47/6811—Drugs conjugated to an antibody or immunoglobulin, e.g. cisplatin-antibody conjugates the drug being a protein or peptide, e.g. transferrin or bleomycin
- A61K47/6815—Enzymes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
- C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
- C07K2317/622—Single chain antibody (scFv)
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Pharmacology & Pharmacy (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Oncology (AREA)
- Epidemiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
Высокоаффинное моноклональное антитело, причем аффинность отличается следующим:(i) инкубацией первого и второго образцов антитела в лунках покрытого антигеном микротитровального планшета при концентрации, выбираемой из пределов участка линейной зависимости в калибровочной кривой, при pH 7,2 в течение 1 ч при 37°C;(ii) удалением несвязанного антитела из обоих образцов;(iii) инкубацией первого образца в ФСБ при pH 7,2 в течение 1 ч при 37°C и снижение pH у второго образца до pH 3 или ниже и инкубацией в течение 1 ч при 37°C;(iv) удалением несвязанного антитела из обоих образцов;(v) инкубацией обоих образцов с конъюгатом анти-антитела и щелочной фосфатазы в течение 1 ч при 37°C;(vi) удалением несвязанного конъюгата из обоих образцов; и(vii) добавлением к образцам субстрата PNPP, измерением поглощения образцов при 405 нм и определением количества антитела, связанного с антигеном, причем связанное количество во втором образце более чем на 50% больше такового в первом образце.Международная заявка была опубликована вместе с отчетом о международном поиске.A high affinity monoclonal antibody, wherein the affinity differs as follows: (i) incubating the first and second antibody samples in the wells of an antigen-coated microtiter plate at a concentration selected from the limits of the linear dependence portion of the calibration curve, at pH 7.2 for 1 hour at 37 ° C (ii) removing unbound antibody from both samples; (iii) incubating the first sample in PBS at pH 7.2 for 1 hour at 37 ° C and lowering the pH of the second sample to pH 3 or below and incubating for 1 hour at 37 ° C; (iv) removing unbound antibody of both samples; (v) incubating both samples with anti-antibody conjugate and alkaline phosphatase for 1 hour at 37 ° C; (vi) removing unbound conjugate from both samples; and (vii) adding PNPP to the substrate samples, measuring the absorbance of the samples at 405 nm and determining the amount of antibody bound to the antigen, with the associated amount in the second sample more than 50% greater than that in the first sample. The international application was published international search.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB9818915.2A GB9818915D0 (en) | 1998-08-28 | 1998-08-28 | Antibodies |
PCT/GB1999/002729 WO2000012556A1 (en) | 1998-08-28 | 1999-08-20 | High-affinity antibodies |
Publications (1)
Publication Number | Publication Date |
---|---|
EA200100287A1 true EA200100287A1 (en) | 2001-08-27 |
Family
ID=10838074
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EA200100287A EA200100287A1 (en) | 1998-08-28 | 1999-08-20 | HIGH-AFFINITY ANTIBODIES |
Country Status (21)
Country | Link |
---|---|
EP (1) | EP1107990A1 (en) |
JP (1) | JP2002525082A (en) |
KR (1) | KR20010089174A (en) |
CN (1) | CN1315966A (en) |
AU (1) | AU5435099A (en) |
BG (1) | BG105294A (en) |
BR (1) | BR9913303A (en) |
CA (1) | CA2340865A1 (en) |
EA (1) | EA200100287A1 (en) |
GB (1) | GB9818915D0 (en) |
HR (1) | HRP20010149A2 (en) |
HU (1) | HUP0103233A3 (en) |
ID (1) | ID28905A (en) |
IL (1) | IL141523A0 (en) |
MX (1) | MXPA01002248A (en) |
NO (1) | NO20011006D0 (en) |
PL (1) | PL346472A1 (en) |
TR (1) | TR200100643T2 (en) |
WO (1) | WO2000012556A1 (en) |
YU (1) | YU15401A (en) |
ZA (1) | ZA200101573B (en) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB0031284D0 (en) * | 2000-12-21 | 2001-01-31 | Ks Biomedix Ltd | High affinity antibodies |
GB0112844D0 (en) * | 2001-05-25 | 2001-07-18 | Psimei Pharmaceuticals Plc | Neutron capture therapy |
US20030232401A1 (en) * | 2002-06-12 | 2003-12-18 | Pugia Michael J. | Bacterial test method by glycated label binding |
AU2003256266A1 (en) * | 2002-06-12 | 2003-12-31 | Genencor International, Inc. | Methods and compositions for milieu-dependent binding of a targeted agent to a target |
EP1691763A4 (en) * | 2003-12-12 | 2008-03-12 | Genencor Int | Cab molecules |
CN111848790B (en) * | 2020-08-07 | 2022-02-22 | 上海交通大学 | Bovine-derived single-chain antibody for resisting staphylococcus aureus and preparation and application thereof |
CN112724255A (en) * | 2021-01-28 | 2021-04-30 | 成都金昆生物科技有限公司 | Small molecule antibodies targeting carcinoembryonic antigens |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5081235A (en) * | 1989-07-26 | 1992-01-14 | City Of Hope | Chimeric anti-cea antibody |
GB9014932D0 (en) * | 1990-07-05 | 1990-08-22 | Celltech Ltd | Recombinant dna product and method |
-
1998
- 1998-08-28 GB GBGB9818915.2A patent/GB9818915D0/en not_active Ceased
-
1999
- 1999-08-20 BR BR9913303-2A patent/BR9913303A/en not_active IP Right Cessation
- 1999-08-20 ID IDW20010714A patent/ID28905A/en unknown
- 1999-08-20 PL PL99346472A patent/PL346472A1/en not_active Application Discontinuation
- 1999-08-20 TR TR2001/00643T patent/TR200100643T2/en unknown
- 1999-08-20 CA CA002340865A patent/CA2340865A1/en not_active Abandoned
- 1999-08-20 JP JP2000571062A patent/JP2002525082A/en active Pending
- 1999-08-20 CN CN99810367A patent/CN1315966A/en active Pending
- 1999-08-20 HU HU0103233A patent/HUP0103233A3/en unknown
- 1999-08-20 IL IL14152399A patent/IL141523A0/en unknown
- 1999-08-20 MX MXPA01002248A patent/MXPA01002248A/en unknown
- 1999-08-20 AU AU54350/99A patent/AU5435099A/en not_active Abandoned
- 1999-08-20 WO PCT/GB1999/002729 patent/WO2000012556A1/en not_active Application Discontinuation
- 1999-08-20 YU YU15401A patent/YU15401A/en unknown
- 1999-08-20 EA EA200100287A patent/EA200100287A1/en unknown
- 1999-08-20 EP EP99940355A patent/EP1107990A1/en not_active Withdrawn
- 1999-08-20 KR KR1020017002434A patent/KR20010089174A/en not_active Application Discontinuation
-
2001
- 2001-02-26 ZA ZA200101573A patent/ZA200101573B/en unknown
- 2001-02-26 BG BG105294A patent/BG105294A/en unknown
- 2001-02-27 NO NO20011006A patent/NO20011006D0/en not_active Application Discontinuation
- 2001-02-28 HR HR20010149A patent/HRP20010149A2/en not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
HUP0103233A2 (en) | 2001-12-28 |
CN1315966A (en) | 2001-10-03 |
PL346472A1 (en) | 2002-02-11 |
HRP20010149A2 (en) | 2002-02-28 |
IL141523A0 (en) | 2002-03-10 |
BG105294A (en) | 2001-12-29 |
NO20011006L (en) | 2001-02-27 |
TR200100643T2 (en) | 2001-07-23 |
HUP0103233A3 (en) | 2003-10-28 |
ZA200101573B (en) | 2002-02-26 |
EP1107990A1 (en) | 2001-06-20 |
MXPA01002248A (en) | 2002-05-08 |
NO20011006D0 (en) | 2001-02-27 |
CA2340865A1 (en) | 2000-03-09 |
YU15401A (en) | 2003-07-07 |
GB9818915D0 (en) | 1998-10-21 |
JP2002525082A (en) | 2002-08-13 |
BR9913303A (en) | 2001-10-09 |
ID28905A (en) | 2001-07-12 |
WO2000012556A1 (en) | 2000-03-09 |
KR20010089174A (en) | 2001-09-29 |
AU5435099A (en) | 2000-03-21 |
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