EA199900101A1 - Очищенные белки, последовательности рекомбинантной днк и способы управления созреванием растений кофе - Google Patents
Очищенные белки, последовательности рекомбинантной днк и способы управления созреванием растений кофеInfo
- Publication number
- EA199900101A1 EA199900101A1 EA199900101A EA199900101A EA199900101A1 EA 199900101 A1 EA199900101 A1 EA 199900101A1 EA 199900101 A EA199900101 A EA 199900101A EA 199900101 A EA199900101 A EA 199900101A EA 199900101 A1 EA199900101 A1 EA 199900101A1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- acc
- synthase
- expression
- plants
- ethylene
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8249—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving ethylene biosynthesis, senescence or fruit development, e.g. modified tomato ripening, cut flower shelf-life
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Nutrition Science (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Tea And Coffee (AREA)
Abstract
Настоящее изобретение относится к очищенным белкам, последовательностям ДНК, кодирующим их экспрессию, и рекомбинантным молекулам ДНК, включая клетки-хозяева, трансформированные с их помощью, для трансформирования растений кофе с целью подавить экспрессию ферментов, необходимых для синтеза этилена. Последовательности ДНК и рекомбинантные молекулы ДНК характеризуются тем, что они кодируют экспрессию ферментов АЦК синтазы (аминоциклопропан-1-карбоксилат синтазы) или АЦК оксидазы, которые участвуют в процессе биосинтеза этилена в растениях кофе. Растения кофе трансформируют векторами, содержащими последовательности ДНК АЦК синтазы и/или АЦК оксидазы, которые кодируют экспрессию соответствующей РНК, которая является антисенсом мРНК для АЦК синтазы и/или АЦК оксидазы. Полученная антисенсная мРНК связывается с мРНК, соответствующей АЦК синтазе и/или АЦК оксидазе, тем самым инактивируя мРНК, кодирующую один или более ферментов в процессе биосинтеза этилена. Указанные последовательности ДНК также могут быть использованы для предотвращения синтеза АЦК синтазы или АЦК оксидазы путем косупрессии. Итогом любой реализации является то, что трансформированные растения становятся не способными синтезировать этилен, причем на другие аспекты их метаболизма влияния не оказывается.Международная заявка была опубликована вместе с отчетом о международном поиске.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/695,412 US5874269A (en) | 1995-06-07 | 1996-08-12 | Purified proteins, recombinant DNA sequences and processes for controlling the ripening of coffee plant |
PCT/US1997/014184 WO1998006852A1 (en) | 1996-08-12 | 1997-08-11 | Purified proteins, recombinant dna sequences and processes for controlling the ripening of coffee plants |
Publications (2)
Publication Number | Publication Date |
---|---|
EA199900101A1 true EA199900101A1 (ru) | 1999-08-26 |
EA004060B1 EA004060B1 (ru) | 2003-12-25 |
Family
ID=24792877
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EA199900101A EA004060B1 (ru) | 1996-08-12 | 1997-08-11 | Очищенные аминоциклопропан-1-карбоксилат-синтаза и аминоциклопропан-1-карбоксилат-оксидаза растения кофе, кодирующие их нуклеиновые кислоты, способы трансформации клеток растения кофе и трансформированные клетки растения кофе |
Country Status (18)
Country | Link |
---|---|
US (1) | US5874269A (ru) |
EP (1) | EP0918869A1 (ru) |
JP (3) | JP2000500663A (ru) |
KR (2) | KR20060035579A (ru) |
CN (1) | CN1227606A (ru) |
AP (1) | AP9901461A0 (ru) |
AU (1) | AU735202B2 (ru) |
BR (1) | BR9710888A (ru) |
CA (1) | CA2260765C (ru) |
CZ (1) | CZ45099A3 (ru) |
EA (1) | EA004060B1 (ru) |
HU (1) | HUP9904413A3 (ru) |
IS (1) | IS4957A (ru) |
NO (1) | NO990508D0 (ru) |
NZ (1) | NZ334117A (ru) |
OA (1) | OA11021A (ru) |
TR (1) | TR199900588T2 (ru) |
WO (1) | WO1998006852A1 (ru) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6448474B1 (en) | 1995-06-07 | 2002-09-10 | University Of Hawaii | Purified proteins, recombinant DNA sequences and processes for controlling the ripening of coffee plants |
AUPP557298A0 (en) | 1998-08-31 | 1998-09-17 | University Of Queensland, The | A novel plant promoter and uses therefor |
BR9915148A (pt) * | 1998-11-11 | 2001-08-07 | Produites Nestle S A Soc D | Mananase de coffea arábica |
US6903247B2 (en) * | 2000-02-08 | 2005-06-07 | Cornell Research Foundation, Inc. | Constitutive α-Tubulin promoter from coffee plants and uses thereof |
US6441273B1 (en) | 2000-02-25 | 2002-08-27 | Cornell Research Foundation, Inc. | Constitutive and inducible promoters from coffee plants |
EP1138771A1 (fr) * | 2000-03-30 | 2001-10-04 | Societe Des Produits Nestle S.A. | Endo-Mannanase de café |
US7678556B2 (en) | 2006-11-21 | 2010-03-16 | Cornell Research Foundation, Inc. | Beta-mannanase from coffee berry borer, Hypothenemus hampei, and uses thereof |
US8685735B2 (en) * | 2008-06-23 | 2014-04-01 | Nestec S.A. | Genes for modulating coffee maturation and methods for their use |
BR102012008162B1 (pt) * | 2012-04-09 | 2018-08-14 | Empresa Brasileira De Pesquisa Agropecuária - Embrapa | composições e métodos para modificar a expressão de genes de interesse |
KR101661253B1 (ko) * | 2013-12-16 | 2016-09-30 | 세종대학교산학협력단 | LHT1 유전자의 ACC(1-Aminocyclopropane-1-Carboxylic acid) 수송체로서의 신규한 용도 |
Family Cites Families (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5453566A (en) * | 1986-03-28 | 1995-09-26 | Calgene, Inc. | Antisense regulation of gene expression in plant/cells |
US5356799A (en) * | 1988-02-03 | 1994-10-18 | Pioneer Hi-Bred International, Inc. | Antisense gene systems of pollination control for hybrid seed production |
US5436395A (en) * | 1988-11-07 | 1995-07-25 | Kraft Foods, Inc. | Induction and selection of somaclonal variation in coffee |
KR920701453A (ko) * | 1989-03-17 | 1992-08-11 | 미리엄 디. 멕코나헤이 | 유전자발현의 외부조절 |
US5334529A (en) * | 1989-06-27 | 1994-08-02 | Escagenetics Corporation | Stably transformed coffee plant cells and plantlets |
GB8916213D0 (en) * | 1989-07-14 | 1989-08-31 | Ici Plc | Dna constructs,cells and plants derived therefrom |
US5416250A (en) * | 1989-12-12 | 1995-05-16 | Agritope, Inc. | Genetic control of ethylene biosynthesis in plants using S-adenosylmethionine hydrolase |
GB9018612D0 (en) * | 1990-08-24 | 1990-10-10 | Ici Plc | Dna,constructs,cells and plants derived therefrom |
MX9100993A (es) * | 1990-09-10 | 1992-05-04 | Us Agriculture | Secuencia de adn aislado y enzima acido 1-aminociclopropan-1-carboxilico sintasa,recombinante |
GB9027616D0 (en) * | 1990-12-20 | 1991-02-13 | Ici Plc | Dna,dna constructs,cells and plants derived therefrom |
GB9109063D0 (en) * | 1991-04-26 | 1991-06-12 | Ici Plc | Modification of lignin synthesis in plants |
US5367065A (en) * | 1992-08-10 | 1994-11-22 | The Trustees Of The University Of Pennsylvania | Constitutive triple response gene and mutations |
US5457041A (en) * | 1994-03-25 | 1995-10-10 | Science Applications International Corporation | Needle array and method of introducing biological substances into living cells using the needle array |
US5518988A (en) * | 1994-06-03 | 1996-05-21 | North Carolina State University | Method of counteracting an ethylene response in plants |
EP0779926A1 (en) * | 1994-09-02 | 1997-06-25 | Seminis Vegetable Seeds, Inc. | Transgenic plants expressing acc oxidase genes |
US5633440A (en) * | 1994-12-20 | 1997-05-27 | Dna Plant Technology Corporation | P119 promoters and their uses |
US5998702A (en) * | 1994-12-30 | 1999-12-07 | Seminis Vegetable Seeds, Inc. | Transgenic plants expressing ACC synthase gene |
-
1996
- 1996-08-12 US US08/695,412 patent/US5874269A/en not_active Expired - Fee Related
-
1997
- 1997-08-11 AU AU40629/97A patent/AU735202B2/en not_active Ceased
- 1997-08-11 WO PCT/US1997/014184 patent/WO1998006852A1/en active Application Filing
- 1997-08-11 EA EA199900101A patent/EA004060B1/ru not_active IP Right Cessation
- 1997-08-11 TR TR1999/00588T patent/TR199900588T2/xx unknown
- 1997-08-11 BR BR9710888A patent/BR9710888A/pt not_active Application Discontinuation
- 1997-08-11 CA CA002260765A patent/CA2260765C/en not_active Expired - Fee Related
- 1997-08-11 KR KR1020057015512A patent/KR20060035579A/ko not_active Application Discontinuation
- 1997-08-11 EP EP97938253A patent/EP0918869A1/en not_active Ceased
- 1997-08-11 HU HU9904413A patent/HUP9904413A3/hu unknown
- 1997-08-11 NZ NZ334117A patent/NZ334117A/xx unknown
- 1997-08-11 JP JP10509967A patent/JP2000500663A/ja not_active Withdrawn
- 1997-08-11 CZ CZ99450A patent/CZ45099A3/cs unknown
- 1997-08-11 KR KR1019997001134A patent/KR20000029923A/ko active IP Right Grant
- 1997-08-11 AP APAP/P/1999/001461A patent/AP9901461A0/en unknown
- 1997-08-11 CN CN97197242A patent/CN1227606A/zh active Pending
-
1999
- 1999-01-26 IS IS4957A patent/IS4957A/is unknown
- 1999-02-04 NO NO990508A patent/NO990508D0/no not_active Application Discontinuation
- 1999-02-10 OA OA9900027A patent/OA11021A/en unknown
-
2002
- 2002-06-18 JP JP2002177730A patent/JP2003070368A/ja not_active Withdrawn
-
2005
- 2005-07-29 JP JP2005222204A patent/JP2005323613A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
WO1998006852A1 (en) | 1998-02-19 |
TR199900588T2 (xx) | 1999-07-21 |
CA2260765C (en) | 2008-01-15 |
EA004060B1 (ru) | 2003-12-25 |
CZ45099A3 (cs) | 1999-07-14 |
CA2260765A1 (en) | 1998-02-19 |
EP0918869A1 (en) | 1999-06-02 |
OA11021A (en) | 2002-02-21 |
JP2005323613A (ja) | 2005-11-24 |
BR9710888A (pt) | 1999-08-17 |
NZ334117A (en) | 2000-08-25 |
NO990508L (no) | 1999-02-04 |
IS4957A (is) | 1999-01-26 |
NO990508D0 (no) | 1999-02-04 |
US5874269A (en) | 1999-02-23 |
AU4062997A (en) | 1998-03-06 |
AP9901461A0 (en) | 1999-03-31 |
AU735202B2 (en) | 2001-07-05 |
CN1227606A (zh) | 1999-09-01 |
JP2000500663A (ja) | 2000-01-25 |
KR20000029923A (ko) | 2000-05-25 |
HUP9904413A2 (hu) | 2000-05-28 |
JP2003070368A (ja) | 2003-03-11 |
KR20060035579A (ko) | 2006-04-26 |
HUP9904413A3 (en) | 2002-01-28 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
MM4A | Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s) |
Designated state(s): AM AZ KZ KG MD TJ TM |
|
MM4A | Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s) |
Designated state(s): BY RU |