DK3916108T3 - Fremgangsmåde til rumlig tagging og analyse af nukleinsyrer i en biologisk prøve - Google Patents

Fremgangsmåde til rumlig tagging og analyse af nukleinsyrer i en biologisk prøve Download PDF

Info

Publication number
DK3916108T3
DK3916108T3 DK21177621.6T DK21177621T DK3916108T3 DK 3916108 T3 DK3916108 T3 DK 3916108T3 DK 21177621 T DK21177621 T DK 21177621T DK 3916108 T3 DK3916108 T3 DK 3916108T3
Authority
DK
Denmark
Prior art keywords
analysis
procedure
nucleic acids
biological sample
tagging
Prior art date
Application number
DK21177621.6T
Other languages
English (en)
Inventor
Jonas Frisén
Joakim Lundeberg
Fredrik Salmén
Sanja Vickovic
Patrik Ståhl
Original Assignee
Spatial Transcriptomics Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Spatial Transcriptomics Ab filed Critical Spatial Transcriptomics Ab
Application granted granted Critical
Publication of DK3916108T3 publication Critical patent/DK3916108T3/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6841In situ hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6823Release of bound markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK21177621.6T 2016-11-17 2017-11-17 Fremgangsmåde til rumlig tagging og analyse af nukleinsyrer i en biologisk prøve DK3916108T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1619458.1A GB201619458D0 (en) 2016-11-17 2016-11-17 Method for spatial tagging and analysing nucleic acids in a biological specimen
EP17809218.5A EP3541956B1 (en) 2016-11-17 2017-11-17 Method for spatial tagging and analysing nucleic acids in a biological specimen

Publications (1)

Publication Number Publication Date
DK3916108T3 true DK3916108T3 (da) 2023-06-12

Family

ID=57993969

Family Applications (2)

Application Number Title Priority Date Filing Date
DK21177621.6T DK3916108T3 (da) 2016-11-17 2017-11-17 Fremgangsmåde til rumlig tagging og analyse af nukleinsyrer i en biologisk prøve
DK17809218.5T DK3541956T3 (da) 2016-11-17 2017-11-17 Fremgangsmåde til rumlig mærkning og analyse af nukleinsyrer i en biologisk prøve

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK17809218.5T DK3541956T3 (da) 2016-11-17 2017-11-17 Fremgangsmåde til rumlig mærkning og analyse af nukleinsyrer i en biologisk prøve

Country Status (5)

Country Link
EP (3) EP3916108B1 (da)
CN (2) CN110249057B (da)
DK (2) DK3916108T3 (da)
GB (1) GB201619458D0 (da)
WO (1) WO2018091676A1 (da)

Families Citing this family (173)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2555106T3 (es) 2010-04-05 2015-12-29 Prognosys Biosciences, Inc. Ensayos biológicos codificados espacialmente
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
BR112015003354A8 (pt) 2012-08-14 2018-01-16 10X Genomics Inc métodos e composições de microcápsula
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN105849275B (zh) 2013-06-25 2020-03-17 普罗格诺西斯生物科学公司 检测样品中生物靶标的空间分布的方法和***
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
CN113249435A (zh) 2014-06-26 2021-08-13 10X基因组学有限公司 分析来自单个细胞或细胞群体的核酸的方法
KR102321863B1 (ko) 2015-01-12 2021-11-08 10엑스 제노믹스, 인크. 핵산 시퀀싱 라이브러리의 제조 방법 및 시스템 및 이를 이용하여 제조한 라이브러리
ES2972835T3 (es) 2015-04-10 2024-06-17 10X Genomics Sweden Ab Análisis multiplex de especímenes biológicos de ácidos nucleicos espacialmente distinguidos
EP3325650B1 (en) 2015-07-17 2024-01-03 Nanostring Technologies, Inc. Simultaneous quantification of gene expression in a user-defined region of a cross-sectioned tissue
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
SG11201804086VA (en) 2015-12-04 2018-06-28 10X Genomics Inc Methods and compositions for nucleic acid analysis
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP4029939B1 (en) 2017-01-30 2023-06-28 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
EP3700672B1 (en) 2017-10-27 2022-12-28 10X Genomics, Inc. Methods for sample preparation and analysis
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
SG11202007686VA (en) 2018-02-12 2020-09-29 10X Genomics Inc Methods characterizing multiple analytes from individual cells or cell populations
KR20200121329A (ko) 2018-02-12 2020-10-23 나노스트링 테크놀로지스, 인크. 유전자 및 단백질 발현을 검출하는 생체 분자 프로브 및 방법
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2019169028A1 (en) 2018-02-28 2019-09-06 10X Genomics, Inc. Transcriptome sequencing through random ligation
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
US20210317524A1 (en) 2018-08-28 2021-10-14 10X Genomics, Inc. Resolving spatial arrays
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
EP3844304A2 (en) 2018-08-28 2021-07-07 10X Genomics, Inc. Methods for generating spatially barcoded arrays
WO2020123301A2 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Generating spatial arrays with gradients
GB201820300D0 (en) 2018-12-13 2019-01-30 10X Genomics Inc Method for spatial tagging and analysing genomic DNA in a biological specimen
GB201820341D0 (en) 2018-12-13 2019-01-30 10X Genomics Inc Method for transposase-mediated spatial tagging and analysing genomic DNA in a biological specimen
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) * 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
WO2020168013A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
EP3931354A1 (en) 2019-02-28 2022-01-05 10X Genomics, Inc. Profiling of biological analytes with spatially barcoded oligonucleotide arrays
EP3938537A1 (en) 2019-03-11 2022-01-19 10X Genomics, Inc. Systems and methods for processing optically tagged beads
CN114127309A (zh) 2019-03-15 2022-03-01 10X基因组学有限公司 使用空间阵列进行单细胞测序的方法
WO2020198071A1 (en) 2019-03-22 2020-10-01 10X Genomics, Inc. Three-dimensional spatial analysis
JP2022533226A (ja) * 2019-05-20 2022-07-21 エンコディア, インコーポレイテッド 空間分析のための方法および関連キット
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
CN117036248A (zh) 2019-10-01 2023-11-10 10X基因组学有限公司 用于识别组织样品中的形态学模式的***和方法
CN110804654A (zh) * 2019-10-30 2020-02-18 东南大学 一种空间转录组测序方法
US20210130881A1 (en) 2019-11-06 2021-05-06 10X Genomics, Inc. Imaging system hardware
EP4055185A1 (en) 2019-11-08 2022-09-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
EP4058572A4 (en) * 2019-11-11 2023-11-29 The Broad Institute, Inc. HIGH RESOLUTION SPATIAL AND QUANTITATIVE DNA ASSESSMENT
US20230002812A1 (en) 2019-11-13 2023-01-05 10X Genomics, Inc. Generating capture probes for spatial analysis
US20210150707A1 (en) 2019-11-18 2021-05-20 10X Genomics, Inc. Systems and methods for binary tissue classification
WO2021102039A1 (en) 2019-11-21 2021-05-27 10X Genomics, Inc, Spatial analysis of analytes
WO2021102005A1 (en) 2019-11-22 2021-05-27 10X Genomics, Inc. Systems and methods for spatial analysis of analytes using fiducial alignment
CN111073872B (zh) * 2019-12-20 2022-04-22 北京优迅医疗器械有限公司 Dna损伤修复体系、dna文库构建试剂盒及文库构建方法
ES2946357T3 (es) 2019-12-23 2023-07-17 10X Genomics Inc Métodos para el análisis espacial usando ligación con molde de ARN
WO2021142233A1 (en) 2020-01-10 2021-07-15 10X Genomics, Inc. Methods for determining a location of a target nucleic acid in a biological sample
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US20230047782A1 (en) 2020-02-07 2023-02-16 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
US20230081381A1 (en) 2020-02-20 2023-03-16 10X Genomics, Inc. METHODS TO COMBINE FIRST AND SECOND STRAND cDNA SYNTHESIS FOR SPATIAL ANALYSIS
EP4107282A1 (en) 2020-02-21 2022-12-28 10X Genomics, Inc. Capturing genetic targets using a hybridization approach
CN115698324A (zh) 2020-02-21 2023-02-03 10X基因组学有限公司 用于整合原位空间测定的方法和组合物
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
ES2965354T3 (es) 2020-04-22 2024-04-12 10X Genomics Inc Métodos para análisis espacial que usan eliminación de ARN elegido como diana
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
GB202007059D0 (en) * 2020-05-13 2020-06-24 Univ Oxford Innovation Ltd Polynucleotide arrays
WO2021236625A1 (en) 2020-05-19 2021-11-25 10X Genomics, Inc. Electrophoresis cassettes and instrumentation
EP4414459A2 (en) 2020-05-22 2024-08-14 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021237056A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Rna integrity analysis in a biological sample
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
AU2021283184A1 (en) 2020-06-02 2023-01-05 10X Genomics, Inc. Spatial transcriptomics for antigen-receptors
EP4025692A2 (en) 2020-06-02 2022-07-13 10X Genomics, Inc. Nucleic acid library methods
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
US20230279474A1 (en) 2020-06-10 2023-09-07 10X Genomics, Inc. Methods for spatial analysis using blocker oligonucleotides
WO2021252591A1 (en) 2020-06-10 2021-12-16 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
AU2021294334A1 (en) 2020-06-25 2023-02-02 10X Genomics, Inc. Spatial analysis of DNA methylation
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
WO2022008649A1 (en) 2020-07-08 2022-01-13 F. Hoffmann-La Roche Ag Spatial analysis of multiple targets in tissue samples
CN111793672A (zh) * 2020-07-23 2020-10-20 生捷科技(杭州)有限公司 一种在基因芯片上形成交联倒置探针的方法
US20230287475A1 (en) 2020-07-31 2023-09-14 10X Genomics, Inc. De-crosslinking compounds and methods of use for spatial analysis
WO2022032195A2 (en) 2020-08-06 2022-02-10 Singular Genomics Systems, Inc. Spatial sequencing
WO2022032194A1 (en) 2020-08-06 2022-02-10 Singular Genomics Systems, Inc. Methods for in situ transcriptomics and proteomics
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US20220064718A1 (en) * 2020-08-31 2022-03-03 Applied Materials, Inc. Alignment beads for mfish
CN116547388A (zh) 2020-09-15 2023-08-04 10X基因组学有限公司 从基材中释放延伸的捕获探针的方法及其应用
AU2021345133A1 (en) 2020-09-16 2023-03-30 10X Genomics, Inc. Methods of determining the location of an analyte in a biological sample using a plurality of wells
WO2022061150A2 (en) 2020-09-18 2022-03-24 10X Geonomics, Inc. Sample handling apparatus and image registration methods
WO2022061152A2 (en) 2020-09-18 2022-03-24 10X Genomics, Inc. Sample handling apparatus and fluid delivery methods
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
WO2022081643A2 (en) 2020-10-13 2022-04-21 10X Genomics, Inc. Compositions and methods for generating recombinant antigen binding molecules from single cells
US20240018572A1 (en) 2020-10-22 2024-01-18 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification
AU2021376399A1 (en) 2020-11-06 2023-06-15 10X Genomics, Inc. Compositions and methods for binding an analyte to a capture probe
AU2021385065A1 (en) 2020-11-18 2023-06-15 10X Genomics, Inc. Methods and compositions for analyzing immune infiltration in cancer stroma to predict clinical outcome
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
US20220186300A1 (en) 2020-12-11 2022-06-16 10X Genomics, Inc. Methods and compositions for multimodal in situ analysis
AU2021409136A1 (en) 2020-12-21 2023-06-29 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
US20240076723A1 (en) 2020-12-30 2024-03-07 10X Genomics, Inc. Cleavage of capture probes for spatial analysis
EP4271511A1 (en) 2020-12-30 2023-11-08 10X Genomics, Inc. Molecular array generation using photoresist
WO2022147005A1 (en) 2020-12-30 2022-07-07 10X Genomics, Inc. Methods for analyte capture determination
EP4284942B1 (en) 2021-01-29 2024-08-14 10X Genomics, Inc. Method for transposase mediated spatial tagging and analyzing genomic dna in a biological sample
AU2022227563A1 (en) 2021-02-23 2023-08-24 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
AU2022238446A1 (en) 2021-03-18 2023-09-07 10X Genomics, Inc. Multiplex capture of gene and protein expression from a biological sample
WO2022216688A1 (en) 2021-04-05 2022-10-13 10X Genomics, Inc. Recombinant ligase composition and uses thereof
WO2022221425A1 (en) 2021-04-14 2022-10-20 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
WO2022226057A1 (en) 2021-04-20 2022-10-27 10X Genomics, Inc. Methods for assessing sample quality prior to spatial analysis using templated ligation
WO2022236054A1 (en) 2021-05-06 2022-11-10 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
EP4347879A1 (en) 2021-06-03 2024-04-10 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
WO2022271820A1 (en) 2021-06-22 2022-12-29 10X Genomics, Inc. Spatial detection of sars-cov-2 using templated ligation
EP4352252A1 (en) 2021-07-13 2024-04-17 10X Genomics, Inc. Methods for spatial analysis using targeted probe silencing
WO2023288225A1 (en) 2021-07-13 2023-01-19 10X Genomics, Inc. Methods for preparing polymerized matrix with controllable thickness
EP4370675A1 (en) 2021-08-12 2024-05-22 10X Genomics, Inc. Methods, compositions and systems for identifying antigen-binding molecules
EP4196605A1 (en) 2021-09-01 2023-06-21 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
WO2023044071A1 (en) 2021-09-17 2023-03-23 10X Genomics, Inc. Systems and methods for image registration or alignment
WO2023076345A1 (en) 2021-10-26 2023-05-04 10X Genomics, Inc. Methods for spatial analysis using targeted rna capture
AU2022387613A1 (en) 2021-11-10 2024-05-30 10X Genomics, Inc. Methods for identification of antigen-binding molecules
WO2023086880A1 (en) 2021-11-10 2023-05-19 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
CN114276993A (zh) * 2021-11-11 2022-04-05 上海昆盟生物科技有限公司 一种用于分离大鼠下丘脑弓状核神经元的方法
US20230167495A1 (en) 2021-11-30 2023-06-01 10X Genomics, Inc. Systems and methods for identifying regions of aneuploidy in a tissue
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
CN114250224B (zh) * 2021-12-16 2024-02-27 福建和瑞基因科技有限公司 一种用于提取或检测样本中小分子rna的核酸组合物及其试剂盒和方法
WO2023122033A1 (en) 2021-12-20 2023-06-29 10X Genomics, Inc. Self-test for pathology/histology slide imaging device
WO2023150098A1 (en) 2022-02-01 2023-08-10 10X Genomics, Inc. Methods, kits, compositions, and systems for spatial analysis
WO2023150163A1 (en) 2022-02-01 2023-08-10 10X Genomics, Inc. Methods, compositions, and systems for capturing analytes from lymphatic tissue
WO2023150171A1 (en) 2022-02-01 2023-08-10 10X Genomics, Inc. Methods, compositions, and systems for capturing analytes from glioblastoma samples
US20230306593A1 (en) 2022-02-15 2023-09-28 10X Genomics, Inc. Systems and methods for spatial analysis of analytes using fiducial alignment
WO2023172670A2 (en) 2022-03-11 2023-09-14 10X Genomics, Inc. Sample handling apparatus and fluid delivery methods
WO2023178279A2 (en) * 2022-03-18 2023-09-21 Bio-Rad Laboratories, Inc. Methods and compositions for maximum release of oligonucleotides
WO2023201235A2 (en) 2022-04-12 2023-10-19 10X Genomics, Inc. Compositions and methods for generating and characterizing recombinant antigen binding molecules
WO2023215552A1 (en) 2022-05-06 2023-11-09 10X Genomics, Inc. Molecular barcode readers for analyte detection
US20240002902A1 (en) 2022-05-06 2024-01-04 10X Genomics, Inc. Analysis of antigen and antigen receptor interactions
WO2023225519A1 (en) 2022-05-17 2023-11-23 10X Genomics, Inc. Modified transposons, compositions and uses thereof
WO2023229988A1 (en) 2022-05-23 2023-11-30 10X Genomics, Inc. Tissue sample mold
WO2023229982A2 (en) 2022-05-24 2023-11-30 10X Genomics, Inc. Porous structure confinement for convection suppression
WO2023250077A1 (en) 2022-06-22 2023-12-28 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
WO2024006816A1 (en) 2022-06-29 2024-01-04 10X Genomics, Inc. Compositions and methods for oligonucleotide inversion on arrays
US20240084359A1 (en) 2022-06-29 2024-03-14 10X Genomics, Inc. Methods and compositions for patterned molecular array generation by directed bead delivery
WO2024006797A1 (en) 2022-06-29 2024-01-04 10X Genomics, Inc. Methods and compositions for refining feature boundaries in molecular arrays
EP4370242A1 (en) 2022-06-29 2024-05-22 10X Genomics, Inc. Compositions and methods for generating molecular arrays using oligonucleotide printing and photolithography
US20240060127A1 (en) 2022-06-29 2024-02-22 10X Genomics, Inc. Methods and systems for light-controlled surface patterning using photomasks
US20240076721A1 (en) 2022-06-29 2024-03-07 10X Genomics, Inc. Method of generating arrays using microfluidics and photolithography
WO2024006799A1 (en) 2022-06-29 2024-01-04 10X Genomics, Inc. Covalent attachment of splint oligonucleotides for molecular array generation using ligation
EP4405094A1 (en) 2022-06-29 2024-07-31 10X Genomics, Inc. High definition molecular array feature generation using photoresist
WO2024015862A1 (en) 2022-07-13 2024-01-18 10X Genomics, Inc. Methods for characterization of antigen-binding molecules from biological samples
WO2024015578A1 (en) 2022-07-15 2024-01-18 10X Genomics, Inc. Methods for determining a location of a target nucleic acid in a biological sample
US20240052404A1 (en) 2022-08-05 2024-02-15 10X Genomics, Inc. Systems and methods for immunofluorescence quantification
WO2024036191A1 (en) 2022-08-10 2024-02-15 10X Genomics, Inc. Systems and methods for colocalization
WO2024035844A1 (en) 2022-08-12 2024-02-15 10X Genomics, Inc. Methods for reducing capture of analytes
WO2024044703A1 (en) 2022-08-24 2024-02-29 10X Genomics, Inc. Compositions and methods for antigenic epitope mapping in biological samples
WO2024081212A1 (en) 2022-10-10 2024-04-18 10X Genomics, Inc. In vitro transcription of spatially captured nucleic acids
US20240191297A1 (en) 2022-10-14 2024-06-13 10X Genomics, Inc. Methods, compositions, and systems for assessing biological sample quality
WO2024078585A1 (zh) * 2022-10-14 2024-04-18 深圳赛陆医疗科技有限公司 在载体上生成探针的方法和样品中靶物质的检测方法
WO2024086167A2 (en) 2022-10-17 2024-04-25 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
WO2024102809A1 (en) 2022-11-09 2024-05-16 10X Genomics, Inc. Methods, compositions, and kits for determining the location of multiple analytes in a biological sample
WO2024137826A1 (en) 2022-12-21 2024-06-27 10X Genomics, Inc. Analysis of analytes and spatial gene expression
WO2024145224A1 (en) 2022-12-29 2024-07-04 10X Genomics, Inc. Compositions, methods, and systems for high resolution spatial analysis
WO2024145441A1 (en) 2022-12-29 2024-07-04 10X Genomics, Inc. Methods, compositions, and kits for determining a location of a target nucleic acid in a fixed biological sample
WO2024145445A1 (en) 2022-12-30 2024-07-04 10X Genomics, Inc. Methods of capturing target analytes
WO2024145491A1 (en) 2022-12-30 2024-07-04 10X Genomics, Inc. Methods, compositions, and kits for multiple barcoding and/or high-density spatial barcoding
CN116536399A (zh) * 2023-07-07 2023-08-04 之江实验室 一种基于微珠测序的基因芯片编解码方法及基因芯片

Family Cites Families (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4883867A (en) 1985-11-01 1989-11-28 Becton, Dickinson And Company Detection of reticulocytes, RNA or DNA
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
EP0450060A1 (en) 1989-10-26 1991-10-09 Sri International Dna sequencing
DE69219610T2 (de) 1991-09-16 1997-10-02 Molecular Probes Inc Dimere unsymmetrische Cyaninfarbstoffe
US5321130A (en) 1992-02-10 1994-06-14 Molecular Probes, Inc. Unsymmetrical cyanine dyes with a cationic side chain
US5410030A (en) 1993-04-05 1995-04-25 Molecular Probes, Inc. Dimers of unsymmetrical cyanine dyes containing pyridinium moieties
US5436134A (en) 1993-04-13 1995-07-25 Molecular Probes, Inc. Cyclic-substituted unsymmetrical cyanine dyes
US5658751A (en) 1993-04-13 1997-08-19 Molecular Probes, Inc. Substituted unsymmetrical cyanine dyes with selected permeability
US5512462A (en) 1994-02-25 1996-04-30 Hoffmann-La Roche Inc. Methods and reagents for the polymerase chain reaction amplification of long DNA sequences
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
US6327410B1 (en) 1997-03-14 2001-12-04 The Trustees Of Tufts College Target analyte sensors utilizing Microspheres
US6023540A (en) 1997-03-14 2000-02-08 Trustees Of Tufts College Fiber optic sensor with encoded microspheres
US7060431B2 (en) 1998-06-24 2006-06-13 Illumina, Inc. Method of making and decoding of array sensors with microspheres
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
WO2000063437A2 (en) 1999-04-20 2000-10-26 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US6770441B2 (en) 2000-02-10 2004-08-03 Illumina, Inc. Array compositions and methods of making same
CN101525660A (zh) 2000-07-07 2009-09-09 维西根生物技术公司 实时序列测定
GB0018120D0 (en) 2000-07-24 2000-09-13 Fermentas Ab Nuclease
WO2002044425A2 (en) 2000-12-01 2002-06-06 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
AU2003259350A1 (en) 2002-08-23 2004-03-11 Solexa Limited Modified nucleotides for polynucleotide sequencing
EP1790202A4 (en) 2004-09-17 2013-02-20 Pacific Biosciences California APPARATUS AND METHOD FOR ANALYZING MOLECULES
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
CA2648149A1 (en) 2006-03-31 2007-11-01 Solexa, Inc. Systems and devices for sequence by synthesis analysis
US8343746B2 (en) 2006-10-23 2013-01-01 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
EP2677309B9 (en) 2006-12-14 2014-11-19 Life Technologies Corporation Methods for sequencing a nucleic acid using large scale FET arrays, configured to measure a limited pH range
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
GB201106254D0 (en) * 2011-04-13 2011-05-25 Frisen Jonas Method and product
EP3901280A1 (en) * 2012-10-17 2021-10-27 Spatial Transcriptomics AB Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
EP2969479B1 (en) 2013-03-13 2021-05-05 Illumina, Inc. Multilayer fluidic devices and methods for their fabrication
ES2972835T3 (es) 2015-04-10 2024-06-17 10X Genomics Sweden Ab Análisis multiplex de especímenes biológicos de ácidos nucleicos espacialmente distinguidos
US20180057873A1 (en) * 2015-04-17 2018-03-01 Centrillion Technology Holdings Corporation Methods for performing spatial profiling of biological materials
CA3176469A1 (en) * 2015-07-27 2017-02-02 Illumina, Inc. Spatial mapping of nucleic acid sequence information

Also Published As

Publication number Publication date
CN117965695A (zh) 2024-05-03
CN110249057B (zh) 2024-01-30
EP3541956A1 (en) 2019-09-25
EP3916108A1 (en) 2021-12-01
EP3541956B1 (en) 2021-07-07
EP4148145A1 (en) 2023-03-15
EP4148145B1 (en) 2024-04-10
GB201619458D0 (en) 2017-01-04
CN110249057A (zh) 2019-09-17
DK3541956T3 (da) 2021-09-13
EP4148145C0 (en) 2024-04-10
EP3916108B1 (en) 2023-03-29
WO2018091676A1 (en) 2018-05-24

Similar Documents

Publication Publication Date Title
DK3916108T3 (da) Fremgangsmåde til rumlig tagging og analyse af nukleinsyrer i en biologisk prøve
DK3615690T3 (da) Sammensætninger og fremgangsmåder til forbedring af prøveidentificering i indekserede nukleinsyrebiblioteker
DK3882357T3 (da) Fremgangsmåder og sammensætninger til analyse af nukleinsyrer
DK3548652T3 (da) Fremgangsmåder til proteinanalyse
ZA201901986B (en) Devices and methods for sample analysis
ZA201901985B (en) Devices and methods for sample analysis
DK3350342T3 (da) Sondesæt til analyse af en DNA-prøve og fremgangsmåde til anvendelse deraf
IL267582A (en) Reagents and Methods for Linked Nucleic Acid Analysis
DK3080585T3 (da) Biosensorer til biologisk eller kemisk analyse og fremgangsmåder til fremstilling heraf
IL253860B (en) Devices for the collection and analysis of biological samples and methods for their use
DK3274692T3 (da) Fremgangsmåder til billeddannelse af prøver til biologisk eller kemisk analyse
GB201616174D0 (en) Method and apparatus for analysing a biological sample
DK3302804T3 (da) Prøveholder og analysesystem til udførelse af specifikke reaktioner
SG11201700602WA (en) Method and device for determining a ratio of free nucleic acids in a biological sample and use thereof
BR112015019614A2 (pt) análise de imagens e medições de amostras biológicas
DK3411151T3 (da) Mikrofluidt assaysystem og fremgangsmåde til at udføre et assay
DK3145634T3 (da) Prøveudtagnings- og analysekit, prøveholder og fremgangsmåde
FR3026745B1 (fr) Methode d'analyse et interpretation automatisee d'un antibiogramme
DK3523035T3 (da) Analyseanordning og fremgangmåde til at teste en prøve
GB2558482B (en) Biological sample analyzer and biological sample analysis method
DK3207374T3 (da) Fremgangsmåde og system til bestemmelse af koncentrationen af en analyt i en fluidprøve
FR3019473B1 (fr) Methode d'analyse microbiologique d'un echantillon dans un conteneur unique
SG11201607148QA (en) Urine sample analysis method and reagent kit for urine sample analysis
DK3523448T3 (da) Fremgangsmåde og analysesystem til at teste en prøve
DK3377894T3 (da) Fremgangsmåde til lægemiddel-bindingsanalyse i en vævsprøve