DK3604525T3 - Fremgangsmåde til tilvejebringelse af et dna-indkodet bibliotek, dna-inkodet bibliotek og fremgangsmåde til at afkode et dna-indkodet bibliotek - Google Patents

Fremgangsmåde til tilvejebringelse af et dna-indkodet bibliotek, dna-inkodet bibliotek og fremgangsmåde til at afkode et dna-indkodet bibliotek Download PDF

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Publication number
DK3604525T3
DK3604525T3 DK18186948.8T DK18186948T DK3604525T3 DK 3604525 T3 DK3604525 T3 DK 3604525T3 DK 18186948 T DK18186948 T DK 18186948T DK 3604525 T3 DK3604525 T3 DK 3604525T3
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Denmark
Prior art keywords
dna
encoded library
decoding
providing
library
Prior art date
Application number
DK18186948.8T
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English (en)
Inventor
Meiying Cui
Helena Andrade
Stephan Heiden
Francesco Reddavide
Yixin Zhang
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Univ Dresden Tech
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Publication of DK3604525T3 publication Critical patent/DK3604525T3/da

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1065Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1089Design, preparation, screening or analysis of libraries using computer algorithms
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00Libraries per se, e.g. arrays, mixtures
    • C40B40/04Libraries containing only organic compounds
    • C40B40/06Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/08Liquid phase synthesis, i.e. wherein all library building blocks are in liquid phase or in solution during library creation; Particular methods of cleavage from the liquid support
    • C40B50/10Liquid phase synthesis, i.e. wherein all library building blocks are in liquid phase or in solution during library creation; Particular methods of cleavage from the liquid support involving encoding steps
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B70/00Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Structural Engineering (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK18186948.8T 2018-08-02 2018-08-02 Fremgangsmåde til tilvejebringelse af et dna-indkodet bibliotek, dna-inkodet bibliotek og fremgangsmåde til at afkode et dna-indkodet bibliotek DK3604525T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP18186948.8A EP3604525B1 (en) 2018-08-02 2018-08-02 Method for providing a dna-encoded library, dna-encoded library and method of decoding a dna-encoded library

Publications (1)

Publication Number Publication Date
DK3604525T3 true DK3604525T3 (da) 2021-05-31

Family

ID=63293916

Family Applications (1)

Application Number Title Priority Date Filing Date
DK18186948.8T DK3604525T3 (da) 2018-08-02 2018-08-02 Fremgangsmåde til tilvejebringelse af et dna-indkodet bibliotek, dna-inkodet bibliotek og fremgangsmåde til at afkode et dna-indkodet bibliotek

Country Status (4)

Country Link
US (1) US20200040326A1 (da)
EP (1) EP3604525B1 (da)
JP (1) JP7397464B2 (da)
DK (1) DK3604525T3 (da)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113373524B (zh) * 2020-05-11 2022-10-25 南京世和基因生物技术股份有限公司 一种ctDNA测序标签接头、文库、检测方法和试剂盒
CN114411267B (zh) * 2020-10-28 2024-04-16 成都先导药物开发股份有限公司 一种On-DNA反应构建β-脂肪取代酮类化合物的方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201218909D0 (en) * 2012-10-22 2012-12-05 Univ Singapore Assay for the parallel detection of biological material based on PCR
CA2889862C (en) 2012-11-05 2021-02-16 Rubicon Genomics, Inc. Barcoding nucleic acids
DE102014213783B3 (de) * 2014-07-16 2015-06-18 Technische Universität Dresden Verfahren zur Identifizierung hochaffiner Komplexe aus zwei Liganden und einem Rezeptor, Vorrichtung zur Durchführung des Verfahrens und selbst-assemblierende chemische Bibliothek zur Verwendung in dem Verfahren
US11996168B2 (en) * 2015-10-28 2024-05-28 The Broad Institute, Inc. Systems and methods for determining relative abundances of biomolecules
WO2018005720A1 (en) * 2016-06-30 2018-01-04 President And Fellows Of Harvard College Method of determining the molecular binding between libraries of molecules
EP3545106B1 (en) * 2017-08-01 2022-01-19 Helitec Limited Methods of enriching and determining target nucleotide sequences

Also Published As

Publication number Publication date
JP7397464B2 (ja) 2023-12-13
US20200040326A1 (en) 2020-02-06
JP2020054337A (ja) 2020-04-09
EP3604525A1 (en) 2020-02-05
EP3604525B1 (en) 2021-03-10

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