DK3208335T3 - Fremgangsmåde til brydning af nukleinsyre og tilføjelse af adapter ved hjælp af transposase og reagens - Google Patents

Fremgangsmåde til brydning af nukleinsyre og tilføjelse af adapter ved hjælp af transposase og reagens Download PDF

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Publication number
DK3208335T3
DK3208335T3 DK14903871.3T DK14903871T DK3208335T3 DK 3208335 T3 DK3208335 T3 DK 3208335T3 DK 14903871 T DK14903871 T DK 14903871T DK 3208335 T3 DK3208335 T3 DK 3208335T3
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Denmark
Prior art keywords
transposase
adaptation
reagents
procedure
nucleic acid
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DK14903871.3T
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English (en)
Inventor
Chunyu Geng
Ruoying Chen
Rongrong Guo
Andrei Alexeev
Yingxin Zhang
Hui Jiang
Wenwei Zhang
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Mgi Tech Co Ltd
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Publication of DK3208335T3 publication Critical patent/DK3208335T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
DK14903871.3T 2014-10-14 2014-10-14 Fremgangsmåde til brydning af nukleinsyre og tilføjelse af adapter ved hjælp af transposase og reagens DK3208335T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2014/088542 WO2016058127A1 (zh) 2014-10-14 2014-10-14 一种转座酶打断核酸并加接头的方法和试剂

Publications (1)

Publication Number Publication Date
DK3208335T3 true DK3208335T3 (da) 2020-08-31

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Family Applications (1)

Application Number Title Priority Date Filing Date
DK14903871.3T DK3208335T3 (da) 2014-10-14 2014-10-14 Fremgangsmåde til brydning af nukleinsyre og tilføjelse af adapter ved hjælp af transposase og reagens

Country Status (7)

Country Link
US (1) US20170292153A1 (da)
EP (1) EP3208335B1 (da)
JP (1) JP2017534297A (da)
CN (1) CN106715691B (da)
DK (1) DK3208335T3 (da)
ES (1) ES2821434T3 (da)
WO (1) WO2016058127A1 (da)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112018076259A2 (pt) * 2017-02-21 2019-03-26 Illumina, Inc. tagmentação usando transposomas imobilizados com ligantes
EP3643787A4 (en) * 2017-06-20 2021-01-06 MGI Tech Co., Ltd. PCR PRIMER PAIR AND USE OF IT
WO2021232184A1 (zh) * 2020-05-18 2021-11-25 深圳华大智造科技有限公司 标签化的转座复合体及其在高通量测序中的应用

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
CN101125873A (zh) * 1997-10-24 2008-02-20 茵维特罗根公司 利用具重组位点的核酸进行重组克隆
NZ520579A (en) * 1997-10-24 2004-08-27 Invitrogen Corp Recombinational cloning using nucleic acids having recombination sites and methods for synthesizing double stranded nucleic acids
US9080211B2 (en) * 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
ES2515065T3 (es) * 2008-10-24 2014-10-29 Epicentre Technologies Corporation Composiciones de extremo del transposón y métodos para modificar ácidos nucleicos
CA2821559C (en) * 2011-01-28 2017-01-31 Illumina, Inc. Oligonucleotide replacement for di-tagged and directional libraries
CN103443338B (zh) * 2011-02-02 2017-09-22 华盛顿大学商业化中心 大规模平行邻接作图
US10689643B2 (en) * 2011-11-22 2020-06-23 Active Motif, Inc. Targeted transposition for use in epigenetic studies
NO2694769T3 (da) * 2012-03-06 2018-03-03
CN102703426A (zh) * 2012-05-21 2012-10-03 吴江汇杰生物科技有限公司 构建核酸库的方法、试剂及试剂盒
AU2013302867A1 (en) * 2012-08-13 2015-02-26 The Regents Of The University Of California Methods and systems for detecting biological components
US9644199B2 (en) * 2012-10-01 2017-05-09 Agilent Technologies, Inc. Immobilized transposase complexes for DNA fragmentation and tagging
CN104372414B (zh) * 2012-10-25 2016-05-04 盛司潼 一种构建测序文库的方法

Also Published As

Publication number Publication date
EP3208335B1 (en) 2020-07-01
CN106715691B (zh) 2020-06-02
EP3208335A1 (en) 2017-08-23
CN106715691A (zh) 2017-05-24
ES2821434T3 (es) 2021-04-26
US20170292153A1 (en) 2017-10-12
EP3208335A4 (en) 2018-04-18
JP2017534297A (ja) 2017-11-24
WO2016058127A1 (zh) 2016-04-21

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