DK3167052T3 - P450-bm3-varianter med forbedret aktivitet - Google Patents
P450-bm3-varianter med forbedret aktivitet Download PDFInfo
- Publication number
- DK3167052T3 DK3167052T3 DK15818953.0T DK15818953T DK3167052T3 DK 3167052 T3 DK3167052 T3 DK 3167052T3 DK 15818953 T DK15818953 T DK 15818953T DK 3167052 T3 DK3167052 T3 DK 3167052T3
- Authority
- DK
- Denmark
- Prior art keywords
- variations
- improved activity
- activity
- improved
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/001—Amines; Imines
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/795—Porphyrin- or corrin-ring-containing peptides
- C07K14/80—Cytochromes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
- C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
- C12N9/0042—NADPH-cytochrome P450 reductase (1.6.2.4)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
- C12Y114/14—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen (1.14.14)
- C12Y114/14001—Unspecific monooxygenase (1.14.14.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y106/00—Oxidoreductases acting on NADH or NADPH (1.6)
- C12Y106/02—Oxidoreductases acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
- C12Y106/02004—NADPH-hemoprotein reductase (1.6.2.4), i.e. NADP-cytochrome P450-reductase
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462022442P | 2014-07-09 | 2014-07-09 | |
| PCT/US2015/039550 WO2016007623A1 (en) | 2014-07-09 | 2015-07-08 | Novel p450-bm3 variants with improved activity |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3167052T3 true DK3167052T3 (da) | 2020-02-10 |
Family
ID=55064831
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK15818953.0T DK3167052T3 (da) | 2014-07-09 | 2015-07-08 | P450-bm3-varianter med forbedret aktivitet |
Country Status (5)
| Country | Link |
|---|---|
| US (6) | US9708587B2 (da) |
| EP (1) | EP3167052B1 (da) |
| DK (1) | DK3167052T3 (da) |
| IL (1) | IL249511B (da) |
| WO (1) | WO2016007623A1 (da) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK3167052T3 (da) * | 2014-07-09 | 2020-02-10 | Codexis Inc | P450-bm3-varianter med forbedret aktivitet |
| US9683220B2 (en) | 2015-07-07 | 2017-06-20 | Codexis, Inc. | P450-BM3 variants with improved activity |
| SE540670C2 (en) * | 2017-02-15 | 2018-10-09 | Pharem Biotech Ab | Enzymatic purification of toilet water |
| GB201807815D0 (en) * | 2018-05-14 | 2018-06-27 | Hypha Discovery Ltd | Hydroxylation techniques |
| CN111218430B (zh) * | 2018-11-26 | 2022-06-14 | 中国科学院大连化学物理研究所 | 一种nadh类似物依赖型的细胞色素p450还原酶及其应用 |
| GB201819209D0 (en) | 2018-11-26 | 2019-01-09 | Hypha Discovery Ltd | Biocatalytic techniques |
| EP3858986A1 (en) | 2020-02-03 | 2021-08-04 | Bayer Aktiengesellschaft | P450 bm3 monooxygenase variants for c19-hydroxylation of steroids |
| US11525123B2 (en) * | 2020-03-12 | 2022-12-13 | California Institute Of Technology | Diverse carbene transferase enzyme catalysts derived from a P450 enzyme |
Family Cites Families (48)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5538867A (en) | 1988-09-13 | 1996-07-23 | Elf Aquitaine | Process for the electrochemical regeneration of pyridine cofactors |
| US5834252A (en) | 1995-04-18 | 1998-11-10 | Glaxo Group Limited | End-complementary polymerase reaction |
| US6395547B1 (en) | 1994-02-17 | 2002-05-28 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US6335160B1 (en) | 1995-02-17 | 2002-01-01 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
| US5928905A (en) | 1995-04-18 | 1999-07-27 | Glaxo Group Limited | End-complementary polymerase reaction |
| US6995017B1 (en) | 1994-02-17 | 2006-02-07 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US5837458A (en) | 1994-02-17 | 1998-11-17 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
| US6117679A (en) | 1994-02-17 | 2000-09-12 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US6309883B1 (en) | 1994-02-17 | 2001-10-30 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| US6165793A (en) | 1996-03-25 | 2000-12-26 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US20060257890A1 (en) | 1996-05-20 | 2006-11-16 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
| US6406855B1 (en) | 1994-02-17 | 2002-06-18 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
| DK0765394T3 (da) | 1994-06-03 | 2001-12-10 | Novozymes As | Oprensede Myceliopthora-laccaser og nukleinsyrer der koder for disse |
| AU2705895A (en) | 1994-06-30 | 1996-01-25 | Novo Nordisk Biotech, Inc. | Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein |
| FI104465B (fi) | 1995-06-14 | 2000-02-15 | Valio Oy | Proteiinihydrolysaatteja allergioiden hoitamiseksi tai estämiseksi, niiden valmistus ja käyttö |
| US6506602B1 (en) | 1996-03-25 | 2003-01-14 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US6096548A (en) | 1996-03-25 | 2000-08-01 | Maxygen, Inc. | Method for directing evolution of a virus |
| EP1717322B1 (en) | 1997-01-17 | 2012-07-18 | Codexis Mayflower Holdings, LLC | Evolution of whole cells and organisms by recursive sequence recombination |
| US6326204B1 (en) | 1997-01-17 | 2001-12-04 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
| US7148054B2 (en) | 1997-01-17 | 2006-12-12 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
| US6537746B2 (en) | 1997-12-08 | 2003-03-25 | Maxygen, Inc. | Method for creating polynucleotide and polypeptide sequences |
| US6365408B1 (en) | 1998-06-19 | 2002-04-02 | Maxygen, Inc. | Methods of evolving a polynucleotides by mutagenesis and recombination |
| JP4221100B2 (ja) | 1999-01-13 | 2009-02-12 | エルピーダメモリ株式会社 | 半導体装置 |
| US6368861B1 (en) | 1999-01-19 | 2002-04-09 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
| US6436675B1 (en) | 1999-09-28 | 2002-08-20 | Maxygen, Inc. | Use of codon-varied oligonucleotide synthesis for synthetic shuffling |
| US6376246B1 (en) | 1999-02-05 | 2002-04-23 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
| US6917882B2 (en) | 1999-01-19 | 2005-07-12 | Maxygen, Inc. | Methods for making character strings, polynucleotides and polypeptides having desired characteristics |
| ATE465459T1 (de) | 1999-01-19 | 2010-05-15 | Maxygen Inc | Durch oligonukleotide-vermittelte nukleinsäuren- rekombination |
| US7702464B1 (en) | 2001-08-21 | 2010-04-20 | Maxygen, Inc. | Method and apparatus for codon determining |
| US7024312B1 (en) | 1999-01-19 | 2006-04-04 | Maxygen, Inc. | Methods for making character strings, polynucleotides and polypeptides having desired characteristics |
| US6961664B2 (en) | 1999-01-19 | 2005-11-01 | Maxygen | Methods of populating data structures for use in evolutionary simulations |
| AU3391900A (en) | 1999-03-05 | 2000-09-21 | Maxygen, Inc. | Encryption of traits using split gene sequences |
| US7430477B2 (en) | 1999-10-12 | 2008-09-30 | Maxygen, Inc. | Methods of populating data structures for use in evolutionary simulations |
| US6519065B1 (en) | 1999-11-05 | 2003-02-11 | Jds Fitel Inc. | Chromatic dispersion compensation device |
| WO2001075767A2 (en) | 2000-03-30 | 2001-10-11 | Maxygen, Inc. | In silico cross-over site selection |
| US7783428B2 (en) | 2002-03-01 | 2010-08-24 | Maxygen, Inc. | Methods, systems, and software for identifying functional biomolecules |
| US20050084907A1 (en) | 2002-03-01 | 2005-04-21 | Maxygen, Inc. | Methods, systems, and software for identifying functional biomolecules |
| US7747391B2 (en) | 2002-03-01 | 2010-06-29 | Maxygen, Inc. | Methods, systems, and software for identifying functional biomolecules |
| AU2003213846A1 (en) | 2002-03-09 | 2003-09-29 | Maxygen, Inc. | Optimization of crossover points for directed evolution |
| US7524664B2 (en) * | 2003-06-17 | 2009-04-28 | California Institute Of Technology | Regio- and enantioselective alkane hydroxylation with modified cytochrome P450 |
| US20080268517A1 (en) | 2007-02-08 | 2008-10-30 | The California Institute Of Technology | Stable, functional chimeric cytochrome p450 holoenzymes |
| GB0719620D0 (en) | 2007-10-08 | 2007-11-14 | Isis Innovation | Mutant Enzymes |
| US20090312196A1 (en) | 2008-06-13 | 2009-12-17 | Codexis, Inc. | Method of synthesizing polynucleotide variants |
| ES2575005T3 (es) | 2008-06-13 | 2016-06-23 | Codexis, Inc. | Método de síntesis de variantes de polinucleótidos |
| EP2912169A4 (en) * | 2012-10-29 | 2016-11-23 | Univ Rochester | ARTEMISININE DERIVATIVES, METHOD FOR THE PRODUCTION THEREOF AND THEIR USE AS ANTIMALARIAMIDAL |
| DK3167052T3 (da) * | 2014-07-09 | 2020-02-10 | Codexis Inc | P450-bm3-varianter med forbedret aktivitet |
| US9683220B2 (en) * | 2015-07-07 | 2017-06-20 | Codexis, Inc. | P450-BM3 variants with improved activity |
-
2015
- 2015-07-08 DK DK15818953.0T patent/DK3167052T3/da active
- 2015-07-08 US US14/794,249 patent/US9708587B2/en active Active
- 2015-07-08 EP EP15818953.0A patent/EP3167052B1/en active Active
- 2015-07-08 WO PCT/US2015/039550 patent/WO2016007623A1/en active Application Filing
-
2016
- 2016-12-12 IL IL24951116A patent/IL249511B/en active IP Right Grant
-
2017
- 2017-06-09 US US15/619,176 patent/US10100289B2/en active Active
-
2018
- 2018-09-14 US US16/131,252 patent/US10689627B2/en active Active
-
2020
- 2020-05-13 US US15/931,310 patent/US11279917B2/en active Active
-
2022
- 2022-02-15 US US17/672,542 patent/US11807874B2/en active Active
-
2023
- 2023-09-28 US US18/476,753 patent/US20240026313A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| WO2016007623A1 (en) | 2016-01-14 |
| IL249511A0 (en) | 2017-02-28 |
| US20190002847A1 (en) | 2019-01-03 |
| EP3167052A4 (en) | 2018-07-04 |
| US11279917B2 (en) | 2022-03-22 |
| US10689627B2 (en) | 2020-06-23 |
| US11807874B2 (en) | 2023-11-07 |
| US20160010065A1 (en) | 2016-01-14 |
| IL249511B (en) | 2019-11-28 |
| US9708587B2 (en) | 2017-07-18 |
| US20240026313A1 (en) | 2024-01-25 |
| US10100289B2 (en) | 2018-10-16 |
| US20220169994A1 (en) | 2022-06-02 |
| EP3167052B1 (en) | 2020-01-01 |
| EP3167052A1 (en) | 2017-05-17 |
| US20170275598A1 (en) | 2017-09-28 |
| US20200332268A1 (en) | 2020-10-22 |
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