DK3140285T3 - Polymethinforbindelser og anvendelse deraf som fluorescerende markører - Google Patents
Polymethinforbindelser og anvendelse deraf som fluorescerende markører Download PDFInfo
- Publication number
- DK3140285T3 DK3140285T3 DK15723996.3T DK15723996T DK3140285T3 DK 3140285 T3 DK3140285 T3 DK 3140285T3 DK 15723996 T DK15723996 T DK 15723996T DK 3140285 T3 DK3140285 T3 DK 3140285T3
- Authority
- DK
- Denmark
- Prior art keywords
- nucleotide
- nucleotides
- labelled
- dye
- compounds
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D403/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00
- C07D403/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings
- C07D403/06—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
- C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
- C07D209/04—Indoles; Hydrogenated indoles
- C07D209/08—Indoles; Hydrogenated indoles with only hydrogen atoms or radicals containing only hydrogen and carbon atoms, directly attached to carbon atoms of the hetero ring
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
- C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
- C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
- C09B23/08—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines
- C09B23/083—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines five >CH- groups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Claims (7)
1. Forbindelse med formlen (IVd) eller mesomere former deraf:
\ -L hvor mCat+ eller mAn- er en organisk eller uorganisk positivt/negativt ladet modion og m er et heltal 0-3; n er et heltal på 1-5; og q er et heltal fra 1-6.
2. Forbindelse ifølge krav 1 hvor q er 4.
3. Nukleotid eller oligonukleotid labelled with a forbindelse ifølge krav 1-2.
4. Nukleotid eller oligonukleotid ifølge krav 3, hvor nukleotidet eller oligonukleotidet er bundet ved omdannelse af COOH-gruppen til et amid eller en ester.
5. Kit, der omfatter to eller flere nukleotider, hvor mindst ét nukleotid er et mærket nukleotid ifølge krav 3 eller krav 4.
6. Anvendelse af et nukleotid ifølge krav 3 eller krav 4, et oligonukleotid ifølge krav 3 eller krav 4 eller et kit ifølge krav 5 ved sekvensering, ekspressionsanalyse, hybridiseringsanalyse, genetisk analyse, RNA-analyse eller proteinbindings-assays.
7. Fremgangsmåde til syntetisering af en forbindelse ifølge krav 1-2 ved anvendelse af følgende udgangsmateriale:
eller et salt deraf, hvor n er et heltal på 1-5.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB1408077.4A GB201408077D0 (en) | 2014-05-07 | 2014-05-07 | Polymethine compounds and their use as fluorescent labels |
PCT/GB2015/051337 WO2015170102A1 (en) | 2014-05-07 | 2015-05-07 | Polymethine compounds and their use as fluorescent labels |
Publications (1)
Publication Number | Publication Date |
---|---|
DK3140285T3 true DK3140285T3 (da) | 2019-01-21 |
Family
ID=50980745
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK18208484.8T DK3476830T3 (da) | 2014-05-07 | 2015-05-07 | Polymethinforbindelser og anvendelse deraf som fluorescerende markører |
DK15723996.3T DK3140285T3 (da) | 2014-05-07 | 2015-05-07 | Polymethinforbindelser og anvendelse deraf som fluorescerende markører |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK18208484.8T DK3476830T3 (da) | 2014-05-07 | 2015-05-07 | Polymethinforbindelser og anvendelse deraf som fluorescerende markører |
Country Status (10)
Country | Link |
---|---|
US (2) | US9309409B2 (da) |
EP (3) | EP3140285B1 (da) |
CN (1) | CN106459001B (da) |
AU (2) | AU2015257477B2 (da) |
CA (1) | CA2947658C (da) |
DK (2) | DK3476830T3 (da) |
ES (2) | ES2702662T3 (da) |
GB (1) | GB201408077D0 (da) |
TW (1) | TWI622580B (da) |
WO (1) | WO2015170102A1 (da) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2013159042A1 (en) | 2012-04-19 | 2013-10-24 | University Of Washington Through Its Center For Commercialization | Methods and compositions for generating reference maps for nanopore-based polymer analysis |
GB201408077D0 (en) * | 2014-05-07 | 2014-06-18 | Illumina Cambridge Ltd | Polymethine compounds and their use as fluorescent labels |
GB201414098D0 (en) | 2014-08-08 | 2014-09-24 | Illumina Cambridge Ltd | Modified nucleotide linkers |
GB201508858D0 (en) | 2015-05-22 | 2015-07-01 | Illumina Cambridge Ltd | Polymethine compounds with long stokes shifts and their use as fluorescent labels |
GB201516987D0 (en) | 2015-09-25 | 2015-11-11 | Illumina Cambridge Ltd | Polymethine compounds and their use as fluorescent labels |
US20220195516A1 (en) | 2020-12-17 | 2022-06-23 | Illumina Cambridge Limited | Methods, systems and compositions for nucleic acid sequencing |
CN114671843B (zh) * | 2020-12-24 | 2023-07-04 | 郑州思昆生物工程有限公司 | 一种荧光化合物、荧光修饰核苷酸、试剂盒 |
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2014
- 2014-05-07 GB GBGB1408077.4A patent/GB201408077D0/en not_active Ceased
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2015
- 2015-05-06 US US14/705,844 patent/US9309409B2/en active Active
- 2015-05-06 TW TW104114388A patent/TWI622580B/zh active
- 2015-05-07 AU AU2015257477A patent/AU2015257477B2/en active Active
- 2015-05-07 CA CA2947658A patent/CA2947658C/en active Active
- 2015-05-07 EP EP15723996.3A patent/EP3140285B1/en active Active
- 2015-05-07 DK DK18208484.8T patent/DK3476830T3/da active
- 2015-05-07 DK DK15723996.3T patent/DK3140285T3/da active
- 2015-05-07 CN CN201580023977.8A patent/CN106459001B/zh active Active
- 2015-05-07 EP EP21150869.2A patent/EP3828167A1/en active Pending
- 2015-05-07 ES ES15723996T patent/ES2702662T3/es active Active
- 2015-05-07 EP EP18208484.8A patent/EP3476830B1/en active Active
- 2015-05-07 ES ES18208484T patent/ES2856217T3/es active Active
- 2015-05-07 WO PCT/GB2015/051337 patent/WO2015170102A1/en active Application Filing
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2016
- 2016-03-01 US US15/058,017 patent/US10144967B2/en active Active
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2019
- 2019-05-23 AU AU2019203624A patent/AU2019203624B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
WO2015170102A1 (en) | 2015-11-12 |
ES2702662T3 (es) | 2019-03-04 |
EP3476830A1 (en) | 2019-05-01 |
CN106459001B (zh) | 2019-03-15 |
AU2019203624A1 (en) | 2019-06-13 |
CA2947658C (en) | 2022-11-29 |
TW201605796A (zh) | 2016-02-16 |
ES2856217T3 (es) | 2021-09-27 |
EP3828167A1 (en) | 2021-06-02 |
US20150322266A1 (en) | 2015-11-12 |
DK3476830T3 (da) | 2021-02-22 |
TWI622580B (zh) | 2018-05-01 |
AU2015257477B2 (en) | 2019-02-28 |
EP3140285A1 (en) | 2017-03-15 |
CA2947658A1 (en) | 2015-11-12 |
AU2019203624B2 (en) | 2021-01-28 |
AU2015257477A1 (en) | 2016-11-17 |
EP3476830B1 (en) | 2021-01-13 |
CN106459001A (zh) | 2017-02-22 |
EP3140285B1 (en) | 2018-11-28 |
GB201408077D0 (en) | 2014-06-18 |
US9309409B2 (en) | 2016-04-12 |
US10144967B2 (en) | 2018-12-04 |
US20160177388A1 (en) | 2016-06-23 |
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