DK3132023T3 - Metode til at forberede celler til 3D-vævskultur - Google Patents

Metode til at forberede celler til 3D-vævskultur Download PDF

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Publication number
DK3132023T3
DK3132023T3 DK15723435.2T DK15723435T DK3132023T3 DK 3132023 T3 DK3132023 T3 DK 3132023T3 DK 15723435 T DK15723435 T DK 15723435T DK 3132023 T3 DK3132023 T3 DK 3132023T3
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DK
Denmark
Prior art keywords
tissue culture
preparing cells
cells
preparing
culture
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DK15723435.2T
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English (en)
Inventor
Simon Messner
Wolfgang Moritz
Jan Lichtenberg
Jens M Kelm
Original Assignee
Insphero Ag
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Publication date
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Publication of DK3132023T3 publication Critical patent/DK3132023T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0671—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5067—Liver cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK15723435.2T 2014-04-15 2015-04-15 Metode til at forberede celler til 3D-vævskultur DK3132023T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1406716.9A GB201406716D0 (en) 2014-04-15 2014-04-15 Method of preparing cells for 3D tissue culture
PCT/EP2015/058174 WO2015158777A1 (en) 2014-04-15 2015-04-15 Method of preparing cells for 3d tissue culture

Publications (1)

Publication Number Publication Date
DK3132023T3 true DK3132023T3 (da) 2021-08-09

Family

ID=50844988

Family Applications (1)

Application Number Title Priority Date Filing Date
DK15723435.2T DK3132023T3 (da) 2014-04-15 2015-04-15 Metode til at forberede celler til 3D-vævskultur

Country Status (10)

Country Link
US (2) US20170037364A1 (da)
EP (1) EP3132023B1 (da)
JP (1) JP6492106B2 (da)
CN (1) CN106459895B (da)
AU (1) AU2015248875B9 (da)
CA (1) CA2945548C (da)
DK (1) DK3132023T3 (da)
GB (1) GB201406716D0 (da)
SG (1) SG11201608463WA (da)
WO (1) WO2015158777A1 (da)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6758026B2 (ja) * 2015-04-17 2020-09-23 株式会社日立ハイテク 成分分析装置、薬剤成分分析装置、成分分析方法及び薬剤成分分析方法
WO2017117333A1 (en) * 2015-12-30 2017-07-06 Cellular Dynamics International, Inc. Microtissue formation using stem cell-derived human hepatocytes
GB201609663D0 (en) 2016-06-02 2016-07-20 Stemtek Therapeutics Sl Methods for producing cancer stem cell spheroids
EP3592842A1 (en) * 2017-03-10 2020-01-15 Merck Patent GmbH Infected cell cultures
CN109294971A (zh) * 2018-09-29 2019-02-01 西安交通大学 一种用于药物筛选的颈动脉分叉三维细胞培养模型及其构建方法
DE102019216886A1 (de) 2019-10-31 2021-05-06 Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. Transfer zumindest eines partikels in einer flüssigkeit von einer abgabeeinheit zu einer empfangseinheit
CN111471641B (zh) * 2020-02-03 2021-11-05 东华大学 多片层单元水凝胶包被的仿生毛细血管网的3d打印制法
CN111150885A (zh) * 2020-03-05 2020-05-15 陕西佰傲干细胞再生医学有限公司 软骨微囊及其制备方法和应用

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2545100B1 (fr) * 1983-04-29 1985-12-20 Inst Nat Sante Rech Med Procede d'obtention de cultures d'hepatocytes humains, les cultures obtenues et leurs applications biologiques et biochimiques
WO2003055487A1 (en) * 2002-01-04 2003-07-10 The University Of Adelaide METHOD OF CONTROLLING DAMAGE MEDIATED BY α, β- UNSATURATED ALDEHYDES
KR20040094910A (ko) * 2002-04-03 2004-11-10 아르테셀 사이언스, 인크. 개선된 지방세포 분화된 지방 유래 성체 줄기세포 및 이의용도
JP2004254674A (ja) * 2003-02-25 2004-09-16 Mutsumi Takagi 動物細胞の分化誘導培養方法
EP2348105B1 (en) * 2004-09-24 2018-10-24 Mesoblast, Inc. Multipotential expanded mesenchymal precursor cell progeny (MEMP) and uses thereof
WO2007124023A2 (en) * 2006-04-21 2007-11-01 Wake Forest University Health Sciences Ink-jet printing of tissues
CN101631852A (zh) * 2006-10-23 2010-01-20 人类起源公司 用胎盘细胞群治疗骨缺损的方法和组合物
JP2010518812A (ja) * 2007-02-12 2010-06-03 アンスロジェネシス コーポレーション 接着性胎盤幹細胞由来の肝細胞および軟骨細胞、ならびにｃｄ３４＋、ｃｄ４５−胎盤幹細胞の濃縮細胞集団
EP2367932B1 (en) * 2008-11-19 2019-06-12 Celularity, Inc. Amnion derived adherent cells
WO2010060080A1 (en) * 2008-11-24 2010-05-27 Immunotrex Corporation Three dimensional tissue generation
ES2609006T3 (es) * 2009-10-16 2017-04-18 Rutgers, The State University Of New Jersey Procedimeinto de tratamiento de lesión crónica de tejido nervioso usando una estrategia de terapia celular
EP2806023B1 (en) * 2009-11-30 2015-09-23 Pluristem Ltd. Adherent cells from placenta and use of same in disease treatment
US20110229970A1 (en) * 2010-03-05 2011-09-22 Florida State University Research Foundation Dual-chamber perfusion bioreactor for orthopedic tissue interfaces and methods of use
CN103097517A (zh) * 2010-06-11 2013-05-08 塞拉帝思股份公司 用于提高多能干细胞分化成肝细胞的3维支架
EP2651452A4 (en) * 2010-12-17 2014-05-07 Anthrogenesis Corp TREATMENT OF BACKBONE INJURIES AND TRAUMATIC BRAIN DAMAGES WITH ADHESIVE CELLS DERIVED FROM AMNION
BR112014005746A2 (pt) * 2011-09-12 2017-03-28 Organovo Inc tecidos engendrados para usos em pesquisa in vitro, arranjos destes, e métodos de fabricar os mesmos

Also Published As

Publication number Publication date
WO2015158777A1 (en) 2015-10-22
AU2015248875B2 (en) 2019-04-04
CA2945548C (en) 2019-11-12
EP3132023B1 (en) 2021-06-02
US20170037364A1 (en) 2017-02-09
CA2945548A1 (en) 2015-10-22
CN106459895A (zh) 2017-02-22
JP6492106B2 (ja) 2019-03-27
CN106459895B (zh) 2021-06-01
AU2015248875A1 (en) 2016-10-27
SG11201608463WA (en) 2016-11-29
GB201406716D0 (en) 2014-05-28
US20230235277A1 (en) 2023-07-27
EP3132023A1 (en) 2017-02-22
AU2015248875B9 (en) 2019-05-23
JP2017511146A (ja) 2017-04-20

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