DK2602326T3 - Fremgangsmåder til transficering af nukleinsyrer til levende celler - Google Patents
Fremgangsmåder til transficering af nukleinsyrer til levende celler Download PDFInfo
- Publication number
- DK2602326T3 DK2602326T3 DK13156520.2T DK13156520T DK2602326T3 DK 2602326 T3 DK2602326 T3 DK 2602326T3 DK 13156520 T DK13156520 T DK 13156520T DK 2602326 T3 DK2602326 T3 DK 2602326T3
- Authority
- DK
- Denmark
- Prior art keywords
- cell
- cells
- nucleic acid
- mrna
- recipient
- Prior art date
Links
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
- A61K48/0083—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the administration regime
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Claims (12)
1. In vitro- eller ex v/Vo-fremgangsmåde til at overføre en multigen fænotype fra en donorcelle til en modtagercelle ved: at transficere en modtagercelle med totalt mRNA fra en donorcelle, hvor donorcellen er af den samme art som modtagercellen, hvor fænotypen af donorcellen er forskellig fra fænotypen af modtagercellen, hvor mRNA'erne trænger ind i modtagercellen, hvor mRNA'erne er funktionelle i modtagercellen, og hvor mRNA'erne udløser en ændring i morfologi og fysiologi i modtagercellen, og hvor ændringen giver en fænotype, som er angivende for donorcellen.
2. Fremgangsmåde ifølge krav 1, hvor modtagercellen er en eukaryotisk celle.
3. Fremgangsmåde ifølge krav 1, hvor modtagercellen er en pattedyrscelle.
4. Fremgangsmåde ifølge krav 1, hvor modtagercellen er udvalgt fra gruppen bestående af en epithelial-celle, en neuron, en fibroblast, en embryonisk fibroblast, en keratinocyt, en voksen stamcelle, en embryonisk stamcelle, en astrocyt og en cardiomyocyt.
5. Fremgangsmåde ifølge krav 1, hvor fremgangsmåden yderligere omfatter at transficere modtagercellen med mindst et DNA fra donorcellen.
6. Fremgangsmåde ifølge krav 5, hvor fremgangsmåden yderligere omfatter at transficere modtagercellen med mindst et udvalgt fra gruppen bestående af siRNA, miRNA, hnRNA og tRNA.
7. Fremgangsmåde ifølge krav 1, hvor det totale mRNA eksprimeres af modtagercellen.
8. Fremgangsmåde ifølge krav 1, hvor det totale mRNA isoleres fra en donorcelle.
9. Fremgangsmåde ifølge krav 8, hvor donorcellen er udvalgt fra gruppen bestående af: en astrocyt, en cardiomyocyt, en neonatal cardiomyocyt, en embryonisk stamcelle og en neuron.
10. Fremgangsmåde ifølge krav 1, hvor det totale mRNA fremstilles ved hjælp af en fremgangsmåde, der er udvalgt fra gruppen bestående af: isolering fra en donorcelle, in vitro-transkription eller kemisk syntese.
11. Fremgangsmåde ifølge krav 10, hvor det totale mRNA er in v7fro-transkri-beret.
12. In vitro- eller ex vivo-fremgangsmåde ifølge krav 1, kendetegnet ved at transficere modtagercellen sekventielt med totalt mRNA fra en donorcelle på et første tidspunkt, og derefter at transficere cellen på et andet eller efterfølgende tidspunkt.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US74994105P | 2005-12-13 | 2005-12-13 | |
| EP06849313.9A EP1963514B1 (en) | 2005-12-13 | 2006-12-12 | Methods for phototransfecting nucleic acid into live cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2602326T3 true DK2602326T3 (da) | 2018-12-10 |
Family
ID=38288081
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK18190026.7T DK3467112T3 (da) | 2005-12-13 | 2006-12-12 | Fremgangsmåder til transfektion af nukleinsyrer til levende celler |
| DK13156520.2T DK2602326T3 (da) | 2005-12-13 | 2006-12-12 | Fremgangsmåder til transficering af nukleinsyrer til levende celler |
| DK06849313.9T DK1963514T3 (da) | 2005-12-13 | 2006-12-12 | Fremgangsmåder til fototransferering af nukleinsyre til levende celler |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK18190026.7T DK3467112T3 (da) | 2005-12-13 | 2006-12-12 | Fremgangsmåder til transfektion af nukleinsyrer til levende celler |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK06849313.9T DK1963514T3 (da) | 2005-12-13 | 2006-12-12 | Fremgangsmåder til fototransferering af nukleinsyre til levende celler |
Country Status (5)
| Country | Link |
|---|---|
| US (3) | US10646590B2 (da) |
| EP (3) | EP2602326B1 (da) |
| DK (3) | DK3467112T3 (da) |
| ES (2) | ES2433669T3 (da) |
| WO (1) | WO2007084228A2 (da) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK3467112T3 (da) | 2005-12-13 | 2020-11-30 | Univ Pennsylvania | Fremgangsmåder til transfektion af nukleinsyrer til levende celler |
| US9157066B2 (en) | 2005-12-13 | 2015-10-13 | The Trustees Of The University Of Pennsylvania | Transcriptome transfer produces cellular phenotype conversion |
| US10647960B2 (en) | 2005-12-13 | 2020-05-12 | The Trustees Of The University Of Pennsylvania | Transcriptome transfer produces cellular phenotype conversion |
| WO2010118045A1 (en) * | 2009-04-07 | 2010-10-14 | The Trustees Of The University Of Pennsylvania | Transcriptome transfer produces cellular phenotype conversion |
| GB201009800D0 (en) * | 2010-06-11 | 2010-07-21 | Univ St Andrews | Optical transfection |
| US9717927B2 (en) * | 2013-02-07 | 2017-08-01 | Rocomp Global, L.L.C. | Electromagnetic radiation targeting devices, assemblies, systems and methods |
Family Cites Families (27)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US3711262A (en) | 1970-05-11 | 1973-01-16 | Corning Glass Works | Method of producing optical waveguide fibers |
| DE3483934D1 (de) | 1983-10-13 | 1991-02-21 | Rikagaku Kenkyusho | Verfahren und apparat zum einpflanzen eines fremdstoffes in lebende zelle. |
| US5034506A (en) | 1985-03-15 | 1991-07-23 | Anti-Gene Development Group | Uncharged morpholino-based polymers having achiral intersubunit linkages |
| US5246437A (en) | 1992-04-10 | 1993-09-21 | Abela George S | Cell treatment apparatus and method |
| US5665565A (en) | 1994-07-12 | 1997-09-09 | The University Of Virginia Patent Foundation | Transfection of enteric parasites |
| US6096716A (en) | 1994-12-12 | 2000-08-01 | The Board Of Regents, The University Of Texas System | Liposome-mediated transfection of central nervous system cells |
| US6753161B2 (en) | 1997-03-27 | 2004-06-22 | Oncosis Llc | Optoinjection methods |
| EP1021549A2 (en) * | 1997-09-19 | 2000-07-26 | Sequitur, Inc. | SENSE mRNA THERAPY |
| AU760113C (en) | 1997-11-18 | 2004-04-22 | Pioneer Hi-Bred International, Inc. | Compositions and methods for genetic modification of plants |
| US20040180430A1 (en) | 1999-09-07 | 2004-09-16 | West Michael D. | Methods of restoring telomere length and extending cell lifespan using nuclear transfer |
| DE69923840T2 (de) * | 1999-09-09 | 2006-04-06 | Curevac Gmbh | Transfer von mRNAs unter Verwendung von polykationischen Verbindungen |
| KR101215789B1 (ko) | 2000-03-30 | 2012-12-26 | 화이트헤드 인스티튜트 포 바이오메디칼 리서치 | Ｒｎａ 간섭의 ｒｎａ 서열 특이적인 매개체 |
| JP2002325572A (ja) * | 2000-12-25 | 2002-11-12 | Univ Osaka | 外来物質の導入方法 |
| GB0111015D0 (en) * | 2001-05-04 | 2001-06-27 | Norsk Hydro As | Genetic material |
| EP1270732A1 (en) * | 2001-06-21 | 2003-01-02 | Schuler, Gerold | Improved transfection of eukaryontic cells with linear polynucleotides by electroporation |
| WO2003079883A2 (en) * | 2002-03-27 | 2003-10-02 | Hadasit Medical Research Services And Development Ltd. | Controlled laser treatment for non-invasive tissue alteration, treatment and diagnostics with minimal collateral damage |
| EP1391503A1 (en) * | 2002-08-12 | 2004-02-25 | Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts | A method of cell re-programming by cytoplasmic transfer |
| US20050095578A1 (en) | 2003-10-31 | 2005-05-05 | Koller Manfred R. | Method and apparatus for cell permeabilization |
| JP4681279B2 (ja) * | 2003-11-17 | 2011-05-11 | 独立行政法人科学技術振興機構 | 細胞外物質の細胞内への導入方法 |
| US6973245B2 (en) | 2003-12-30 | 2005-12-06 | Furukawa Electric North America | Optical fiber cables |
| WO2005068495A1 (ja) | 2004-01-13 | 2005-07-28 | Toray Industries, Inc. | クモ糸タンパク質を含む絹糸および該絹糸を産生するカイコ |
| GB0426182D0 (en) | 2004-11-30 | 2004-12-29 | Univ St Andrews | Photoporation of cells |
| US20070087437A1 (en) * | 2005-10-14 | 2007-04-19 | Jifan Hu | Methods for rejuvenating cells in vitro and in vivo |
| US9157066B2 (en) | 2005-12-13 | 2015-10-13 | The Trustees Of The University Of Pennsylvania | Transcriptome transfer produces cellular phenotype conversion |
| DK3467112T3 (da) | 2005-12-13 | 2020-11-30 | Univ Pennsylvania | Fremgangsmåder til transfektion af nukleinsyrer til levende celler |
| US10647960B2 (en) * | 2005-12-13 | 2020-05-12 | The Trustees Of The University Of Pennsylvania | Transcriptome transfer produces cellular phenotype conversion |
| EP2206724A1 (en) | 2005-12-13 | 2010-07-14 | Kyoto University | Nuclear reprogramming factor |
-
2006
- 2006-12-12 DK DK18190026.7T patent/DK3467112T3/da active
- 2006-12-12 EP EP13156520.2A patent/EP2602326B1/en active Active
- 2006-12-12 DK DK13156520.2T patent/DK2602326T3/da active
- 2006-12-12 EP EP06849313.9A patent/EP1963514B1/en active Active
- 2006-12-12 WO PCT/US2006/047480 patent/WO2007084228A2/en active Application Filing
- 2006-12-12 US US12/086,471 patent/US10646590B2/en active Active
- 2006-12-12 ES ES06849313T patent/ES2433669T3/es active Active
- 2006-12-12 EP EP18190026.7A patent/EP3467112B1/en active Active
- 2006-12-12 ES ES13156520T patent/ES2698600T3/es active Active
- 2006-12-12 DK DK06849313.9T patent/DK1963514T3/da active
-
2012
- 2012-03-21 US US13/426,422 patent/US10583204B2/en active Active
-
2020
- 2020-03-10 US US16/814,120 patent/US20200206364A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| ES2433669T3 (es) | 2013-12-12 |
| DK1963514T3 (da) | 2013-11-04 |
| US20200206364A1 (en) | 2020-07-02 |
| WO2007084228A2 (en) | 2007-07-26 |
| US10646590B2 (en) | 2020-05-12 |
| EP3467112B1 (en) | 2020-11-18 |
| DK3467112T3 (da) | 2020-11-30 |
| EP3467112A2 (en) | 2019-04-10 |
| EP1963514B1 (en) | 2013-08-14 |
| US20120135493A1 (en) | 2012-05-31 |
| EP3467112A3 (en) | 2019-05-22 |
| EP1963514A4 (en) | 2010-05-19 |
| ES2698600T3 (es) | 2019-02-05 |
| EP2602326B1 (en) | 2018-08-22 |
| US10583204B2 (en) | 2020-03-10 |
| WO2007084228A3 (en) | 2008-06-12 |
| EP2602326A1 (en) | 2013-06-12 |
| US20120178167A1 (en) | 2012-07-12 |
| EP1963514A2 (en) | 2008-09-03 |
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