DK2553090T3 - Termostabile phytasevarianter - Google Patents

Termostabile phytasevarianter

Info

Publication number
DK2553090T3
DK2553090T3 DK11709966.3T DK11709966T DK2553090T3 DK 2553090 T3 DK2553090 T3 DK 2553090T3 DK 11709966 T DK11709966 T DK 11709966T DK 2553090 T3 DK2553090 T3 DK 2553090T3
Authority
DK
Denmark
Prior art keywords
phytase variants
thermostable phytase
thermostable
variants
phytase
Prior art date
Application number
DK11709966.3T
Other languages
English (en)
Inventor
Lars Kobberoee Skov
Maria Leonardo De
Tomoko Matsui
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of DK2553090T3 publication Critical patent/DK2553090T3/da

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17Amino acids, peptides or proteins
    • A23L33/195Proteins from microorganisms
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • A61K38/16Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46Hydrolases (3)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • C12P7/14Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y301/00Hydrolases acting on ester bonds (3.1)
    • C12Y301/03Phosphoric monoester hydrolases (3.1.3)
    • C12Y301/030083-Phytase (3.1.3.8)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y301/00Hydrolases acting on ester bonds (3.1)
    • C12Y301/03Phosphoric monoester hydrolases (3.1.3)
    • C12Y301/030264-Phytase (3.1.3.26), i.e. 6-phytase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y301/00Hydrolases acting on ester bonds (3.1)
    • C12Y301/03Phosphoric monoester hydrolases (3.1.3)
    • C12Y301/030725-Phytase (3.1.3.72)
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Nutrition Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Physiology (AREA)
  • Animal Husbandry (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DK11709966.3T 2010-03-26 2011-03-25 Termostabile phytasevarianter DK2553090T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP10158027 2010-03-26
PCT/EP2011/054639 WO2011117396A2 (en) 2010-03-26 2011-03-25 Thermostable phytase variants

Publications (1)

Publication Number Publication Date
DK2553090T3 true DK2553090T3 (da) 2019-05-13

Family

ID=44123229

Family Applications (1)

Application Number Title Priority Date Filing Date
DK11709966.3T DK2553090T3 (da) 2010-03-26 2011-03-25 Termostabile phytasevarianter

Country Status (10)

Country Link
US (2) US20130195829A1 (da)
EP (1) EP2553090B1 (da)
CN (1) CN102906254A (da)
AU (1) AU2011231562B2 (da)
BR (1) BR112012023840B1 (da)
CA (2) CA2794095C (da)
DK (1) DK2553090T3 (da)
ES (1) ES2723050T3 (da)
PL (1) PL2553090T3 (da)
WO (1) WO2011117396A2 (da)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT2397486E (pt) 2006-09-21 2015-03-23 Basf Enzymes Llc Fitases, ácidos nucleicos que as codificam e métodos para as preparar e utilizar
GB201102865D0 (en) 2011-02-18 2011-04-06 Danisco Feed additive composition
GB201102857D0 (en) 2011-02-18 2011-04-06 Danisco Feed additive composition
DK2675287T3 (da) 2011-02-18 2016-11-28 Dupont Nutrition Biosci Aps Fodertilsætningssammensætning
EP2729809B1 (en) 2011-07-07 2018-10-17 DuPont Nutrition Biosciences ApS Assay
GB201213801D0 (en) 2012-08-03 2012-09-12 Dupont Nutrition Biosci Aps Feed additive composition
GB201308828D0 (en) 2013-03-12 2013-07-03 Verenium Corp Phytase
GB201308843D0 (en) 2013-03-14 2013-07-03 Verenium Corp Phytase formulation
EP2799531A1 (de) 2013-05-03 2014-11-05 Clariant Produkte (Deutschland) GmbH Einsatz von Phosphatasen zur enzymatischen Entschleimung von Triglyceriden
US10093909B2 (en) 2013-07-25 2018-10-09 Basf Enzymes Llc Phytase
ES2818986T5 (es) 2013-08-09 2024-01-25 Biomadison Inc Ensayo de toxina botulínica con sensibilidad mejorada
CN104450643B (zh) * 2014-12-19 2020-07-28 青岛蔚蓝生物集团有限公司 植酸酶突变体及其应用
TWI591180B (zh) 2016-02-18 2017-07-11 基酵生物科技股份有限公司 提升耐熱性的植酸酶
TWI615471B (zh) * 2016-02-18 2018-02-21 基酵生物科技股份有限公司 提升耐熱性的植酸酶
TWI616532B (zh) * 2016-02-18 2018-03-01 基酵生物科技股份有限公司 提升耐熱性的植酸酶
TWI607088B (zh) * 2016-02-18 2017-12-01 基酵生物科技股份有限公司 提升耐熱性的植酸酶
CN110283803B (zh) * 2016-06-02 2021-08-03 天津科技大学 一种磷脂酶d及其制备磷脂酸、磷脂酰丝氨酸的方法
BR112020016068A2 (pt) 2018-02-08 2020-12-08 Danisco Us Inc. Partículas cera termicamente resistente matriz para encapsulamento de enzima
JP2021521835A (ja) 2018-04-26 2021-08-30 ダニスコ・ユーエス・インク 固体組成物中のフィターゼの安定性を増加させる方法、並びにリン酸塩及びフィターゼを含む顆粒組成物
US20210207112A1 (en) * 2018-05-30 2021-07-08 Nanjing Bestzyme Bio-Engineering Co., Ltd. Phytase mutant
WO2021007379A1 (en) 2019-07-09 2021-01-14 Dupont Nutrition Biosciences Aps Fat coated particulate enzyme compositions
US20220330576A1 (en) 2019-08-16 2022-10-20 Dupont Nutrition Biosciences Aps Compositions for gut health
WO2021046073A1 (en) 2019-09-05 2021-03-11 Dupont Nutrition Biosciences Aps Feed composition
US20220395543A1 (en) 2019-10-21 2022-12-15 Dupont Nutrition Biosciences Aps Compositions for gut health
EP4099838A1 (en) 2020-02-07 2022-12-14 DuPont Nutrition Biosciences ApS Feed compositions for animal health
WO2022034211A1 (en) 2020-08-13 2022-02-17 Novozymes A/S Phytase variants and polynucleotides encoding same
US20240008511A1 (en) 2020-10-16 2024-01-11 Dupont Nutrition Biosciences Feed compositions for animal health
WO2022169933A2 (en) 2021-02-03 2022-08-11 Dupont Nutrition Biosciences Aps Compositions for gut health
KR20240060703A (ko) 2021-09-27 2024-05-08 인터내셔널 엔&에이치 덴마크 에이피에스 사료 첨가제 조성물 및 이의 사용 방법

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US7442398B2 (en) * 2003-03-25 2008-10-28 Republic Of National Fisheries Research And Development Institute Phytase produced from Citrobacter braakii
GB0422052D0 (en) * 2004-10-04 2004-11-03 Dansico As Enzymes
AU2005290934C1 (en) 2004-10-04 2013-05-09 Dupont Nutrition Biosciences Aps Citrobacter freundii phytase and homologues
AR050895A1 (es) 2004-10-04 2006-11-29 Novozymes As Polipeptidos que tienen actividad de fitasa y polinucleotidos que los codifican
BRPI0517539A (pt) * 2004-10-04 2008-10-14 Novozymes As polipeptìdeo isolado, polinucleotìdeo isolado, construto de ácido nucleico, vetor de expressão recombinante, célula hospedeira recombinante, métodos para produzir o polipeptìdeo, e para melhorar o valor nutricional de uma ração animal, célula de planta, parte de planta ou planta transgênica, animal não-humano, transgênico, ou produtos, ou elementos do mesmo, uso de pelo menos um polipeptìdeo, aditivo de ração animal, e, composição de ração animal
MX2008012632A (es) * 2006-04-04 2008-10-13 Novozymes As Variantes de fitasa.
US8540984B2 (en) * 2006-08-03 2013-09-24 Cornell Research Foundation, Inc. Phytases with improved thermal stability
US8101391B2 (en) * 2007-01-30 2012-01-24 Novozymes A/S Polypeptides having phytase activity and polynucleotides encoding same
JP5643083B2 (ja) * 2007-03-26 2014-12-17 ノボザイムス アクティーゼルスカブ ハフニア・フィターゼ
WO2009110933A2 (en) * 2007-12-03 2009-09-11 Syngenta Participations Ag Engineering enzymatically susceptible phytases
PL2342323T3 (pl) * 2008-09-26 2013-11-29 Novozymes As Warianty fitazy z hafnia
FR2947563B1 (fr) * 2009-07-01 2015-08-21 Biomethodes Variants ameliores d'une phytase
US8334124B1 (en) * 2009-09-23 2012-12-18 The United States Of America As Represented By The Secretary Of Agriculture Modified Aspergillus niger phytase
WO2011048046A2 (de) * 2009-10-22 2011-04-28 Basf Se Synthetische phytasevarianten
ES2758795T3 (es) * 2010-03-26 2020-05-06 Novozymes As Variantes de fitasa termoestables
BR112012023808A2 (pt) * 2010-03-26 2015-09-01 Novozymes As Métodos para produzir um variante de fitase, para melhorar o valor nutricional de uma ração animal, para o tratamento de proteínas vegetais, para sintetizar um produto de fermentação, para produzir etanol, polinucleotídeo, construto de ácido nucleíco, vetor de expressão recombinante, célula hospedeira recombinante, planta transgênica, composição, processo para reduzir os níveis de fitase no esterco animal, e, uso do variante de fitase
DK2675287T3 (da) * 2011-02-18 2016-11-28 Dupont Nutrition Biosci Aps Fodertilsætningssammensætning
DK2699675T3 (da) * 2011-04-21 2015-11-30 Basf Se Syntetiske fytasevarianter

Also Published As

Publication number Publication date
ES2723050T3 (es) 2019-08-21
BR112012023840A2 (pt) 2020-07-28
AU2011231562A1 (en) 2012-09-27
WO2011117396A3 (en) 2011-11-24
CA2794095A1 (en) 2011-09-29
EP2553090B1 (en) 2019-02-27
CN102906254A (zh) 2013-01-30
AU2011231562B2 (en) 2015-10-22
EP2553090A2 (en) 2013-02-06
WO2011117396A2 (en) 2011-09-29
CA3105079A1 (en) 2011-09-29
US20150159149A1 (en) 2015-06-11
PL2553090T3 (pl) 2019-08-30
CA2794095C (en) 2021-02-16
US9611469B2 (en) 2017-04-04
US20130195829A1 (en) 2013-08-01
BR112012023840B1 (pt) 2021-06-22

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