DK2471902T3 - Fremstilling af kunstige væv ved hjælp af vævsengineering med agarose-fibrin-biomaterialer - Google Patents
Fremstilling af kunstige væv ved hjælp af vævsengineering med agarose-fibrin-biomaterialer Download PDFInfo
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- DK2471902T3 DK2471902T3 DK10811319.2T DK10811319T DK2471902T3 DK 2471902 T3 DK2471902 T3 DK 2471902T3 DK 10811319 T DK10811319 T DK 10811319T DK 2471902 T3 DK2471902 T3 DK 2471902T3
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Claims (19)
1. In v/fro-fremgangsmåde til fremstilling af et kunstigt væv omfattende: a) tilsætning af et præparat omfattende fibrinogen til en prøve af isolerede celler, b) tilsætning af et antifibrinolytisk middel til det produkt, der fremkommer i trin (a), c) tilsætning af mindst én koagulationsfaktor, en kilde til calcium, thrombin eller en hvilken som helst kombination af ovenstående til det produkt, der fremkommer i trin (b), d) tilsætning af et præparat af et polysaccharid til det produkt, der fremkommer i trin (c), e) dyrkning af isolerede celler i eller på det produkt, der fremkommer i trin (d), og f) induktion af nanostrukturering af det produkt, der fremkommer i trin (e), hvor nanostruktureringsinduktionen omfatter dehydrering og/eller mekanisk komprimering af det produkt, der fremkommer i trin (e), hvor polysaccharidet i trin (d) er agarose.
2. Fremgangsmåde ifølge krav 1, hvor cellerne i trin (a) er fibroblaster eller keratocyt-ter.
3. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 2, der yderligere omfatter et trin (trin b2) mellem trin (b) og (c), hvor et protein tilsættes.
4. Fremgangsmåde ifølge krav 3, hvor det protein, der tilsættes i trin (b2), erfibronek-tin.
5. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 4, der omfatter et trin (d2) mellem trin (d) og (e), som omfatter tilsætning af et præparat omfattende et protein til det produkt, der fremkommer i trin (d).
6. Fremgangsmåde ifølge krav 5, hvor det protein, der tilsættes i trin (d2), er kollagen.
7. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 6, hvor cellerne i trin (e) omfatter navlestrengsstamceller.
8. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 7, hvor cellerne i trin (e) omfatter epitelceller.
9. Fremgangsmåde ifølge krav 1, hvor dehydrering af det produkt, der fremkommer i trin (e), omfatter en fremgangsmåde, der er valgt fra listen omfattende: dræning, fordampning, sugning, kapillærtryk, osmose eller elektroosmose.
10. Fremgangsmåde ifølge krav 9, hvor dehydrering af det produkt, der fremkommer i trin (e) ved hjælp af kapillærtryk, omfatter påføring af et absorberende materiale på det produkt, der fremkommer i trin (e).
11. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 10, hvor den mekaniske kompression i trin (f) omfatter en fremgangsmåde, der er valgt fra listen omfattende: anvendelse af en statisk belastning, anvendelse af hydraulik, anvendelse af en kamskive, anvendelse af én eller flere valser, anvendelse afen ballon, ekstrudering eller centrifugering.
12. Fremgangsmåde ifølge krav 11, hvor anvendelsen afen statisk belastning i trin (f) omfatter placering af en vægt på det produkt, der fremkommer i trin (e).
13. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 12, hvor der mellem trin (e) og trin (f) er et yderligere trin, hvor det produkt, der fremkommer i trin (e), udsættes for luft.
14. Kunstigt væv opnået ved fremgangsmåden ifølge et hvilket som helst af kravene 1 til 13.
15. Anvendelse af det kunstige væv ifølge krav 14 til vurdering af et farmakologisk og/eller kemisk produkt.
16. Kunstigt væv ifølge krav 14 til anvendelse i medicin.
17. Kunstigt væv ifølge krav 14 til anvendelse til delvist eller fuldstændigt at forøge, genoprette eller udskifte den funktionelle aktivitet af et sygt eller beskadiget væv eller organ.
18. Kunstigt væv til anvendelse ifølge krav 17, hvor det beskadigede væv eller organet er valgt fra listen omfattende: hud, blære, urinrør, hornhinde, slimhinde, bindehinde, bugvæg, bindehinde, trommehinde, svælg, strubehoved, tarm, bughinde, ligament, sene, knogle, hjernehinde eller vagina.
19. Farmaceutisk præparat omfattende det kunstige væv ifølge krav 14.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES200930625A ES2353990B1 (es) | 2009-08-25 | 2009-08-25 | Elaboracion de tejidos artificiales mediante ingenieria tisular utilizando biomateriales de fibrina y agarosa. |
ES200930943A ES2362139B1 (es) | 2009-11-02 | 2009-11-02 | Elaboración de tejidos artificiales mediante ingeniería tisular utilizando biomateriales de fibrina, agarosa y colágeno. |
PCT/ES2010/070569 WO2011023843A2 (es) | 2009-08-25 | 2010-08-25 | Elaboración de tejidos artificiales mediante ingeniería tisular utilizando biomateriales de fibrina y agarosa |
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Publication Number | Publication Date |
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DK2471902T3 true DK2471902T3 (da) | 2018-08-06 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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DK10811319.2T DK2471902T3 (da) | 2009-08-25 | 2010-08-25 | Fremstilling af kunstige væv ved hjælp af vævsengineering med agarose-fibrin-biomaterialer |
Country Status (8)
Country | Link |
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US (2) | US20120269776A1 (da) |
EP (1) | EP2471902B9 (da) |
JP (2) | JP6010460B2 (da) |
DK (1) | DK2471902T3 (da) |
ES (1) | ES2677945T3 (da) |
PT (1) | PT2471902T (da) |
TR (1) | TR201809713T4 (da) |
WO (1) | WO2011023843A2 (da) |
Families Citing this family (14)
Publication number | Priority date | Publication date | Assignee | Title |
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EP2594295A1 (en) * | 2011-11-16 | 2013-05-22 | Servicio Andaluz De Salud | Nerve implants based on a compacted biomaterial containing cells |
FR2990106B1 (fr) * | 2012-05-03 | 2014-05-09 | Genoskin | Systeme permettant la maintenance en survie et le transport de biospsies de peau et ses applications |
RU2523342C1 (ru) * | 2013-02-13 | 2014-07-20 | федеральное государственное бюджетное учреждение "Межотраслевой научно-технический комплекс "Микрохирургия глаза" имени академика С.Н. Федорова" Министерства здравоохранения Российской Федерации | Способ кератопротезирования осложненных сосудистых бельм 4-5 категории |
CL2013003066A1 (es) * | 2013-10-22 | 2014-07-25 | Univ Chile | Composicion para tratamiento de heridas porque comprende matriz soporte y células troncalesmesenquiámicas de gelatina de wharton; metodo para tratar heridas que comprende aplicar dicha composicion |
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ES2574655B1 (es) * | 2014-11-21 | 2017-04-20 | Universidad De Granada | Elaboración de tejidos artificiales que comprenden partículas magnéticas |
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KR20230126745A (ko) | 2016-12-07 | 2023-08-30 | 메이오 파운데이션 포 메디칼 에쥬케이션 앤드 리써치 | 망막 색소 상피 이식을 위해 피브린 지지체를 사용하는 방법 및 물질 |
MX2020002697A (es) * | 2017-09-12 | 2020-09-09 | Shilpa Medicare Ltd | Pulverizador de acido tranexamico para la artroplastia de rodilla. |
CA3080691C (en) * | 2017-11-10 | 2022-11-15 | Regenesis Science Co., Ltd. | Method for producing cultured cell, and method for producing therapeutic agent for spinal cord injury disease |
EP3773765B1 (en) * | 2018-04-06 | 2022-07-27 | Fundación Pública Andaluza Progreso Y Salud | Hemostatic efficacy of a nanostructured fibrin agarose hydrogel |
RU2714943C1 (ru) * | 2019-06-04 | 2020-02-21 | Общество с ограниченной ответственностью фирмы "Имтек" | Искусственная роговица, представляющая собой мембрану гетерогенной жесткости на основе коллагена, и способ ее получения и применения |
EP4182441A1 (en) * | 2020-07-17 | 2023-05-24 | National University of Singapore | Gingival tissues and methods of preparation thereof |
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US4600533A (en) * | 1984-12-24 | 1986-07-15 | Collagen Corporation | Collagen membranes for medical use |
JP2858066B2 (ja) * | 1993-04-06 | 1999-02-17 | グンゼ株式会社 | 組織培養法並びにこれに用いる培養基材 |
US6933331B2 (en) * | 1998-05-22 | 2005-08-23 | Nanoproducts Corporation | Nanotechnology for drug delivery, contrast agents and biomedical implants |
US5844087A (en) * | 1996-11-05 | 1998-12-01 | Bayer Corporation | Method and device for delivering fibrin glue |
AT407117B (de) * | 1997-09-19 | 2000-12-27 | Immuno Ag | Fibrinschwamm |
US6056970A (en) * | 1998-05-07 | 2000-05-02 | Genzyme Corporation | Compositions comprising hemostatic compounds and bioabsorbable polymers |
US6946140B1 (en) * | 1999-02-09 | 2005-09-20 | The Research Foundation Of State University Of New York | Methods and compositions for enhancing fibroblast migration |
US6548058B1 (en) * | 1999-07-20 | 2003-04-15 | Epitech, S.A. | Keratinocyte culture and uses thereof |
AT500670A1 (de) * | 1999-05-19 | 2006-02-15 | Bio & Bio Licensing Sa | Arzneimittel zur lokalen anwendung |
JP2004522446A (ja) * | 2001-02-07 | 2004-07-29 | コリア アトミック エネルギー リサーチ インスティテュート | 上皮細胞の分離方法、細胞を前条件付けする方法、およびバイオ人工皮膚もしくは真皮を上皮細胞または条件付けされた細胞を用いて調製する方法 |
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US20030166274A1 (en) * | 2001-11-15 | 2003-09-04 | Hewitt Charles W. | Three-dimensional matrix for producing living tissue equivalents |
AU2003228808A1 (en) * | 2002-05-02 | 2003-11-17 | Regents Of The University Of Minnesota | Fibrin-based biomatrix |
EP1538196A1 (en) * | 2002-08-23 | 2005-06-08 | ASAHI MEDICAL Co., Ltd. | Fibrin-containing composition |
US20040126881A1 (en) * | 2002-09-06 | 2004-07-01 | Vincent Ronfard | Fibrin cell supports and methods of use thereof |
WO2005079821A1 (en) * | 2004-02-13 | 2005-09-01 | Intercytex Limited | Wound healing profile |
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US8287854B2 (en) * | 2005-10-21 | 2012-10-16 | Cellresearch Corporation Pte Ltd | Skin equivalents derived from umbilical cord mesenchymal stem/progenitor cells and umbilical cord epithelial stem/progenitor cells |
FR2896506B1 (fr) * | 2006-01-20 | 2008-04-04 | Essilor Int | Composition polymerisable a base de polyurethane-uree et de copolymeres a blocs et materiau transparent obtenu a partir de celle-ci |
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EP2471902A2 (en) | 2012-07-04 |
PT2471902T (pt) | 2018-08-02 |
EP2471902A4 (en) | 2014-06-25 |
JP2013502915A (ja) | 2013-01-31 |
ES2677945T3 (es) | 2018-08-07 |
JP6010460B2 (ja) | 2016-10-19 |
WO2011023843A2 (es) | 2011-03-03 |
EP2471902B1 (en) | 2018-05-30 |
EP2471902B9 (en) | 2018-12-19 |
WO2011023843A3 (es) | 2011-07-07 |
TR201809713T4 (tr) | 2018-07-23 |
US20120269776A1 (en) | 2012-10-25 |
US20190247541A1 (en) | 2019-08-15 |
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