DK2275556T3 - Hurtig fremgangsmåde til screening af translationsfusionspartnere til fremstilling af rekombinante proteiner og translationsfusionpartnere screenet derfra - Google Patents

Hurtig fremgangsmåde til screening af translationsfusionspartnere til fremstilling af rekombinante proteiner og translationsfusionpartnere screenet derfra

Info

Publication number
DK2275556T3
DK2275556T3 DK10009260T DK10009260T DK2275556T3 DK 2275556 T3 DK2275556 T3 DK 2275556T3 DK 10009260 T DK10009260 T DK 10009260T DK 10009260 T DK10009260 T DK 10009260T DK 2275556 T3 DK2275556 T3 DK 2275556T3
Authority
DK
Denmark
Prior art keywords
fusion partners
translational fusion
screened
recombinant proteins
screening
Prior art date
Application number
DK10009260T
Other languages
English (en)
Inventor
Jung-Hoon Sohn
Eui-Sung Choi
Jung-Hoon Bae
Eung-Suck Lee
Mi-Kyung Shin
Original Assignee
Korea Res Inst Of Bioscience
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020040003610A external-priority patent/KR100626753B1/ko
Priority claimed from KR1020040003957A external-priority patent/KR20050076139A/ko
Application filed by Korea Res Inst Of Bioscience filed Critical Korea Res Inst Of Bioscience
Application granted granted Critical
Publication of DK2275556T3 publication Critical patent/DK2275556T3/da

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1086Preparation or screening of expression libraries, e.g. reporter assays
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52Cytokines; Lymphokines; Interferons
    • C07K14/53Colony-stimulating factor [CSF]
    • C07K14/535Granulocyte CSF; Granulocyte-macrophage CSF
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52Cytokines; Lymphokines; Interferons
    • C07K14/54Interleukins [IL]
    • C07K14/55IL-2
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18Carboxylic ester hydrolases (3.1.1)
    • C12N9/20Triglyceride splitting, e.g. by means of lipase
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/35Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/61Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DK10009260T 2004-01-17 2004-12-30 Hurtig fremgangsmåde til screening af translationsfusionspartnere til fremstilling af rekombinante proteiner og translationsfusionpartnere screenet derfra DK2275556T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR1020040003610A KR100626753B1 (ko) 2004-01-17 2004-01-17 재조합 단백질 생산용 맞춤형 단백질융합인자의 초고속선별 방법
KR1020040003957A KR20050076139A (ko) 2004-01-19 2004-01-19 단백질 분비 생산을 유도하는 단백질융합인자
EP04808645A EP1713930B1 (en) 2004-01-17 2004-12-30 Rapid screening method of translational fusion partners for producing recombinant proteins and translational fusion partners screened therefrom

Publications (1)

Publication Number Publication Date
DK2275556T3 true DK2275556T3 (da) 2013-07-08

Family

ID=34797866

Family Applications (2)

Application Number Title Priority Date Filing Date
DK04808645T DK1713930T3 (da) 2004-01-17 2004-12-30 Hurtig screeningsmetode for translationsfusionspartnere til fremstilling af rekombinante proteiner og translationsfusionspartnere screenet derfra
DK10009260T DK2275556T3 (da) 2004-01-17 2004-12-30 Hurtig fremgangsmåde til screening af translationsfusionspartnere til fremstilling af rekombinante proteiner og translationsfusionpartnere screenet derfra

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DK04808645T DK1713930T3 (da) 2004-01-17 2004-12-30 Hurtig screeningsmetode for translationsfusionspartnere til fremstilling af rekombinante proteiner og translationsfusionspartnere screenet derfra

Country Status (7)

Country Link
US (2) US8673822B2 (da)
EP (2) EP2275556B1 (da)
JP (2) JP5148879B2 (da)
AT (1) ATE486943T1 (da)
DE (1) DE602004029936D1 (da)
DK (2) DK1713930T3 (da)
WO (1) WO2005068658A1 (da)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8673822B2 (en) 2004-01-17 2014-03-18 Korea Research Institute Of Bioscience And Biotechnology Rapid screening method of translational fusion partners for producing recombinant proteins and translational fusion partners screened therefrom
KR20070009269A (ko) * 2005-07-15 2007-01-18 한국생명공학연구원 재조합단백질 생산용 단백질융합인자 라이브러리 및이로부터 획득된 단백질융합인자
KR101431091B1 (ko) * 2008-12-04 2014-08-26 한국생명공학연구원 고분비성 단백질의 스크리닝 및 재조합 단백질의 생산을 위한 융합 파트너로서 그의 용도
US8986956B2 (en) 2010-11-04 2015-03-24 Korea Research Institute Of Bioscience And Biotechnology Method for producing human epidermal growth factor in large volume from yeast
CN104694523A (zh) * 2015-03-21 2015-06-10 江南大学 一种精氨酸脱亚胺酶的胞外表达方法及其应用
WO2023118670A1 (en) * 2021-12-23 2023-06-29 Helsingin Yliopisto Method for screening a signal peptide for efficient expression and secretion of a heterologous polypeptide in mammalian cells

Family Cites Families (26)

* Cited by examiner, † Cited by third party
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WO1984004330A1 (en) * 1983-04-22 1984-11-08 Amgen Secretion of exogenous polypeptides from yeast
FI871919A (fi) * 1986-05-02 1987-11-03 Gist Brocades Nv Sekretionssignalselektionsvektorer foer extracellulaer proteinsyntes hos bacillus-bakterier.
FR2648713B1 (fr) * 1989-06-26 1994-06-10 Commissariat Energie Atomique Proteines hybrides entre une enzyme extracytoplasmique et une autre proteine, leur procede de preparation, ainsi que leur application comme agent de diagnostic
US5212058A (en) * 1990-05-09 1993-05-18 Massachusetts Institute Of Technology Nucleic acid encoding ubiquitin-specific proteases
US5547871A (en) * 1993-01-25 1996-08-20 American Cyanamid Company Heterologous signal sequences for secretion of insect controlling proteins
US5536637A (en) * 1993-04-07 1996-07-16 Genetics Institute, Inc. Method of screening for cDNA encoding novel secreted mammalian proteins in yeast
US5563046A (en) * 1993-08-02 1996-10-08 Celtrix Pharmaceuticals, Inc. Fusion polypeptides and proteins
DE69426289T2 (de) * 1993-12-10 2001-04-12 Korea Institute Of Science And Technology, Seoul/Soul Signalsequenzen für die Secretion heterologer Proteine von Hefe
WO1996023890A1 (fr) * 1995-02-03 1996-08-08 Asahi Glass Company Ltd. Gene de signal de secretion et vecteur d'expression comprenant ce signal
EP1170366A1 (en) 1996-04-24 2002-01-09 Genetics Institute, Inc. Secreted proteins obtained by using Yeast invertase gene as reporter system
CA2252425A1 (en) 1996-04-24 1997-10-30 Genetics Institute, Inc. Yeast invertase gene as reporter system for isolating cytokines
US5952171A (en) * 1996-11-19 1999-09-14 Millennium Biotherapeutics, Inc. Method for identifying genes encoding secreted or membrane-associated proteins
US6136569A (en) * 1997-02-27 2000-10-24 Genentech, Inc. Method of creating a cDNA library enriched in signal sequences
US20060009633A9 (en) * 1997-11-13 2006-01-12 Genset, S.A. Complementary DNAs encoding proteins with signal peptides
US6548633B1 (en) * 1998-12-22 2003-04-15 Genset, S.A. Complementary DNA's encoding proteins with signal peptides
US6150098A (en) * 1998-02-20 2000-11-21 Amgen Inc. Methods for identifying novel secreted mammalian polypeptides
WO1999049028A1 (en) 1998-03-23 1999-09-30 Genentech, Inc. Method of selection for genes encoding secreted and transmembrane proteins
US6753314B1 (en) * 1999-04-01 2004-06-22 Curagen Corporation Protein-protein complexes and methods of using same
AU5838200A (en) * 1999-06-25 2001-01-31 Genset Carnitine carrier related protein-1
WO2001077315A1 (en) * 2000-04-07 2001-10-18 Novozymes A/S Signal sequence trapping
US7029842B2 (en) * 2000-04-07 2006-04-18 Novozymes A/S Signal sequence trapping
US20020127557A1 (en) * 2001-03-09 2002-09-12 Ruoying Tan Method for identification of cDNAs encoding signal peptides
US7314974B2 (en) * 2002-02-21 2008-01-01 Monsanto Technology, Llc Expression of microbial proteins in plants for production of plants with improved properties
US8673822B2 (en) 2004-01-17 2014-03-18 Korea Research Institute Of Bioscience And Biotechnology Rapid screening method of translational fusion partners for producing recombinant proteins and translational fusion partners screened therefrom
KR20070009269A (ko) * 2005-07-15 2007-01-18 한국생명공학연구원 재조합단백질 생산용 단백질융합인자 라이브러리 및이로부터 획득된 단백질융합인자
KR101431091B1 (ko) * 2008-12-04 2014-08-26 한국생명공학연구원 고분비성 단백질의 스크리닝 및 재조합 단백질의 생산을 위한 융합 파트너로서 그의 용도

Also Published As

Publication number Publication date
EP1713930A1 (en) 2006-10-25
EP1713930B1 (en) 2010-11-03
EP2275556B1 (en) 2013-04-24
US8673822B2 (en) 2014-03-18
JP5148879B2 (ja) 2013-02-20
ATE486943T1 (de) 2010-11-15
JP5662363B2 (ja) 2015-01-28
EP1713930A4 (en) 2007-02-28
EP2275556A1 (en) 2011-01-19
US20140220631A1 (en) 2014-08-07
JP2012105675A (ja) 2012-06-07
DK1713930T3 (da) 2011-01-17
DE602004029936D1 (de) 2010-12-16
US9012367B2 (en) 2015-04-21
WO2005068658A1 (en) 2005-07-28
US20070275385A1 (en) 2007-11-29
JP2007517519A (ja) 2007-07-05

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