DK2240595T4 - Glykosyleret proteinekspression i prokaryoter - Google Patents

Glykosyleret proteinekspression i prokaryoter Download PDF

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Publication number
DK2240595T4
DK2240595T4 DK09701421.1T DK09701421T DK2240595T4 DK 2240595 T4 DK2240595 T4 DK 2240595T4 DK 09701421 T DK09701421 T DK 09701421T DK 2240595 T4 DK2240595 T4 DK 2240595T4
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Prior art keywords
prokaryotes
protein expression
glycosylated protein
glycosylated
expression
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DK09701421.1T
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English (en)
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DK2240595T3 (da
Inventor
Matthew Delisa
Cassandra Guarino
Thomas Mansell
Adam Fisher
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Cornell Res Foundation Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1267—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria
    • C07K16/1278—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01132—GDP-Man:Man1GlcNAc2-PP-dolichol alpha-1,3-mannosyltransferase (2.4.1.132)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01142—Chitobiosyldiphosphodolichol beta-mannosyltransferase (2.4.1.142)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01255—Protein O-GlcNAc transferase (2.4.1.255)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01257—GDP-Man:Man2GlcNAc2-PP-dolichol alpha-1,6-mannosyltransferase (2.4.1.257)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/99—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C12Y204/99018—Dolichyl-diphosphooligosaccharide—protein glycotransferase (2.4.99.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y306/00—Hydrolases acting on acid anhydrides (3.6)
    • C12Y306/03—Hydrolases acting on acid anhydrides (3.6) acting on acid anhydrides; catalysing transmembrane movement of substances (3.6.3)
    • C12Y306/03001—Phospholipid-translocating ATPase (3.6.3.1), i.e. Mg2+-ATPase

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK09701421.1T 2008-01-03 2009-01-05 Glykosyleret proteinekspression i prokaryoter DK2240595T4 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US1877208P 2008-01-03 2008-01-03
PCT/US2009/030110 WO2009089154A2 (en) 2008-01-03 2009-01-05 Glycosylated protein expression in prokaryotes

Publications (2)

Publication Number Publication Date
DK2240595T3 DK2240595T3 (da) 2016-03-07
DK2240595T4 true DK2240595T4 (da) 2019-10-07

Family

ID=40853723

Family Applications (1)

Application Number Title Priority Date Filing Date
DK09701421.1T DK2240595T4 (da) 2008-01-03 2009-01-05 Glykosyleret proteinekspression i prokaryoter

Country Status (8)

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US (2) US8999668B2 (da)
EP (1) EP2240595B2 (da)
JP (1) JP5667880B2 (da)
KR (1) KR101589554B1 (da)
CN (2) CN107119095B (da)
CA (1) CA2711307C (da)
DK (1) DK2240595T4 (da)
WO (1) WO2009089154A2 (da)

Families Citing this family (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HUE038456T2 (hu) * 2009-11-19 2018-10-29 Glaxosmithkline Biologicals Sa Bioszintetikus rendszer, amely immunogén poliszaccharidokat termel prokarióta sejtekben
CA2789505C (en) 2010-02-11 2017-12-05 The Governors Of The University Of Alberta N-linked glycan compounds
JP2013539963A (ja) * 2010-09-03 2013-10-31 ザ ガバナーズ オブ ザ ユニバーシティ オブ アルバータ 複数のｎ結合型グリコシル化シークオンを含んでいるペプチド
US10689447B2 (en) * 2011-02-04 2020-06-23 Genentech, Inc. Fc variants and methods for their production
EP2702077A2 (en) 2011-04-27 2014-03-05 AbbVie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
EP2742355B1 (en) * 2011-08-12 2018-12-19 The Governors of the University of Alberta Method of diagnosing bacterial infections using bacterial glycoproteins
WO2013067523A1 (en) * 2011-11-04 2013-05-10 Cornell University A prokaryote-based cell-free system for the synthesis of glycoproteins
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
US9334319B2 (en) 2012-04-20 2016-05-10 Abbvie Inc. Low acidic species compositions
US9181572B2 (en) 2012-04-20 2015-11-10 Abbvie, Inc. Methods to modulate lysine variant distribution
US20150353940A1 (en) 2013-08-05 2015-12-10 Absci, Llc Vectors for use in an inducible coexpression system
CN104603274B (zh) 2012-08-05 2020-08-18 Absci有限责任公司 可诱导共表达***
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
CN103509807B (zh) * 2012-09-14 2015-04-15 山东大学 糖基化的麦芽糖结合蛋白及其制备方法与应用
CA2923957C (en) * 2013-01-18 2021-08-31 London School Of Hygiene And Tropical Medicine Glycosylation method
SG11201507230PA (en) 2013-03-12 2015-10-29 Abbvie Inc Human antibodies that bind human tnf-alpha and methods of preparing the same
US9499614B2 (en) 2013-03-14 2016-11-22 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosaccharides
US20140273163A1 (en) * 2013-03-14 2014-09-18 Glycobia, Inc. Oligosaccharide compositions, glycoproteins and methods to produce the same in prokaryotes
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
CN105392883A (zh) * 2013-03-15 2016-03-09 格里科比亚有限公司 聚唾液酸、血型抗原以及糖蛋白表达
EP2991675B1 (en) * 2013-04-05 2019-07-24 The Governors of the University of Alberta Campylobacter vaccine
US9598667B2 (en) 2013-10-04 2017-03-21 Abbvie Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US20150139988A1 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
CN103740632B (zh) * 2014-01-22 2016-02-10 山东大学 一株重组大肠杆菌及其在制备抗o157:h7的n-糖蛋白疫苗中的应用
US10918712B2 (en) 2014-03-03 2021-02-16 Albert Einstein College Of Medicine Passive transfer of immunity using recombinant herpes simplex virus 2 (HSV-2) vaccine vectors
CN110938604B (zh) 2014-03-03 2024-04-05 阿尔伯特爱因斯坦医学院 重组单纯疱疹病毒2(hsv-2)疫苗载体
WO2016012468A1 (en) 2014-07-21 2016-01-28 Novartis Ag Production of glycoproteins with mammalian-like n-glycans in filamentous fungi
US9616114B1 (en) 2014-09-18 2017-04-11 David Gordon Bermudes Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity
WO2016107819A1 (en) * 2014-12-30 2016-07-07 Glycovaxyn Ag Compositions and methods for protein glycosylation
US10829795B2 (en) 2016-07-14 2020-11-10 Northwestern University Method for rapid in vitro synthesis of glycoproteins via recombinant production of N-glycosylated proteins in prokaryotic cell lysates
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
WO2019002512A2 (en) * 2017-06-30 2019-01-03 Limmatech Biologics Ag GLYCOPROTEINS CUSTOMIZED FROM ENGINEERING AND FULLY FUNCTIONAL
WO2019035916A1 (en) 2017-08-15 2019-02-21 Northwestern University DESIGN OF PROTEIN GLYCOSYLATION SITES BY RAPID EXPRESSION AND CHARACTERIZATION OF N-GLYCOSYLTRANSFERASES
US11530432B2 (en) 2018-03-19 2022-12-20 Northwestern University Compositions and methods for rapid in vitro synthesis of bioconjugate vaccines in vitro via production and N-glycosylation of protein carriers in detoxified prokaryotic cell lysates
US11725224B2 (en) 2018-04-16 2023-08-15 Northwestern University Methods for co-activating in vitro non-standard amino acid (nsAA) incorporation and glycosylation in crude cell lysates
BR112021005634A2 (pt) 2018-09-25 2021-06-29 Absci, Llc métodos de purificação de proteína
CN113658633B (zh) * 2021-07-20 2023-10-20 北京大学 噬菌体宿主属预测方法、装置、设备及存储介质
WO2023114905A1 (en) 2021-12-16 2023-06-22 Absci Corporation Membrane-associated fusion proteins to increase the competency of cells
WO2023114452A1 (en) 2021-12-17 2023-06-22 Absci Corporation Solid-phase screening for high-performing bacterial strains
WO2023122567A1 (en) 2021-12-21 2023-06-29 Absci Corporation Fola selection assay to identify strains with increased soluble target protein expression
WO2023122448A1 (en) 2021-12-23 2023-06-29 Absci Corporation Products and methods for heterologous expression of proteins in a host cell
WO2023129881A1 (en) 2021-12-30 2023-07-06 Absci Corporation Knockout of ptsp gene elevates active gene expression
US20230268026A1 (en) 2022-01-07 2023-08-24 Absci Corporation Designing biomolecule sequence variants with pre-specified attributes
WO2024006269A1 (en) 2022-06-29 2024-01-04 Absci Corporation Affinity screening method
WO2024040020A1 (en) 2022-08-15 2024-02-22 Absci Corporation Quantitative affinity activity specific cell enrichment

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7504493B2 (en) 1997-01-23 2009-03-17 The John Hopkins University Characterization of the yeast transcriptome
MXPA01004982A (es) 1998-11-18 2004-09-27 Neose Technologies Inc Manufactura de bajo costo de oligosacaridos.
WO2003056914A1 (en) 2001-12-27 2003-07-17 Glycofi, Inc. Methods to engineer mammalian-type carbohydrate structures
US7863020B2 (en) 2000-06-28 2011-01-04 Glycofi, Inc. Production of sialylated N-glycans in lower eukaryotes
US20060257399A1 (en) 2000-06-28 2006-11-16 Glycofi, Inc. Immunoglobulins comprising predominantly a Man5GIcNAc2 glycoform
US7029872B2 (en) 2000-06-28 2006-04-18 Glycofi, Inc Methods for producing modified glycoproteins
KR100938026B1 (ko) * 2002-03-07 2010-01-21 아이드게노쉬쉐 테흐니쉐 호흐슐레 쥬리히 원핵 숙주에서 재조합 글리코실화 단백질의 생산방법 및생산계
KR20090110951A (ko) 2002-03-07 2009-10-23 아이드게노쉬쉐 테흐니쉐 호흐슐레 쥬리히 원핵 숙주에서의 재조합 글리코실화 단백질 생산 방법 및 생산계
AU2003254665C1 (en) 2002-08-01 2010-08-19 National Research Council Of Canada Campylobacter glycans and glycopeptides
ATE445709T1 (de) 2002-10-16 2009-10-15 Scripps Research Inst Glycoproteinsynthese
EP2338333B1 (en) * 2003-04-09 2017-09-06 ratiopharm GmbH Glycopegylation methods and proteins/peptides produced by the methods
AU2005238308B8 (en) 2004-04-29 2009-10-08 Glycofi, Inc. Methods for reducing or eliminating alpha-mannosidase resistant glycans in the production of glycoproteins
JP5235657B2 (ja) * 2005-03-24 2013-07-10 バイオジェネリックス ゲーエムベーハー 原核生物における可溶性活性真核生物グリコシルトランスフェラーゼの発現
WO2006107990A2 (en) 2005-04-05 2006-10-12 The Johns Hopkins University Improving protein n-glycosylation of eukaryotic cells using dolichol-linked oligosaccharide synthesis pathway, other n-gylosylation-increasing methods, and engineered hosts expressing products with increased n-glycosylation
EP2853600B1 (en) 2005-05-11 2018-09-19 ETH Zürich Recombinant N-glycosylated proteins from procaryotic cells
JP2009534034A (ja) 2006-04-19 2009-09-24 ノヴォ ノルディスク アクティーゼルスカブ 原核微生物におけるｏ−グリコシル化治療用タンパク質の発現

Also Published As

Publication number Publication date
EP2240595B1 (en) 2015-12-16
EP2240595B2 (en) 2019-07-24
CN107119095B (zh) 2022-07-05
US8999668B2 (en) 2015-04-07
CA2711307C (en) 2020-10-27
CN107119095A (zh) 2017-09-01
EP2240595A2 (en) 2010-10-20
CN101960017A (zh) 2011-01-26
KR20100108420A (ko) 2010-10-06
JP5667880B2 (ja) 2015-02-12
KR101589554B1 (ko) 2016-02-01
WO2009089154A3 (en) 2010-01-14
US20150232861A1 (en) 2015-08-20
JP2011508607A (ja) 2011-03-17
WO2009089154A2 (en) 2009-07-16
DK2240595T3 (da) 2016-03-07
CN101960017B (zh) 2017-06-09
US20110039729A1 (en) 2011-02-17
CA2711307A1 (en) 2009-07-16
US9512434B2 (en) 2016-12-06
EP2240595A4 (en) 2011-06-29

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