DK200000326U3 - An apparatus for cutting objects in uniform orientation and variable thickness - Google Patents
An apparatus for cutting objects in uniform orientation and variable thickness Download PDFInfo
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- DK200000326U3 DK200000326U3 DK200000326U DKBA200000326U DK200000326U3 DK 200000326 U3 DK200000326 U3 DK 200000326U3 DK 200000326 U DK200000326 U DK 200000326U DK BA200000326 U DKBA200000326 U DK BA200000326U DK 200000326 U3 DK200000326 U3 DK 200000326U3
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- cutting
- alginate
- tissue
- variable thickness
- orientation
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Description
i DK 2000 00326 U 3in DK 2000 00326 U 3
Et apparat til skæring af objekter i ensartet orientering og variabel tykkelse AnvendelseA device for cutting objects in uniform orientation and variable thickness Use
Apparatet til skæring af objekter er designet til brug i forskningslaboratorier til 5 eksperimentelle paradigmer der involverer væv. Skæremaskinen kan frembringe vævsskiver af ens orientering og variabel eller ens tykkelse. Skæremaskinen er således anvendelig til stereologisk vævspræparering da den kan frembringe vævsskiver af ens tykkelse og orientering. Skæremaskinen kan også bruges til at frembringe vævsskiver fra friske organer men henblik på elektrofysiologi eller 10 kvantitativ biokemi. Muligheden for orientering tillader vævssnit at blive sammenlignet med billeder fra billeddannende scannings teknikker så som computer tomografi og magnetisk resonans skanning. Alternativt kan man udføre den ønskede skannings metode på polymer indstøbte objekter fulgt af skæring i skæremaskinen.The object cutting device is designed for use in research laboratories for 5 experimental paradigms involving tissue. The cutting machine can produce tissue slices of the same orientation and variable or thickness. The cutting machine is thus useful for stereological tissue preparation as it can produce tissue slices of equal thickness and orientation. The cutting machine can also be used to produce tissue slices from healthy organs but for electrophysiology or quantitative biochemistry. The orientation allows tissue sections to be compared with images from imaging scanning techniques such as computer tomography and magnetic resonance scanning. Alternatively, the desired scanning method can be performed on polymer embedded objects followed by cutting in the cutting machine.
15 Kendt teknik15 Prior Art
Et apparat til orienteret skæring af vævsblokke og organer er beskrevet i PCT ansøgning No WO 00/37918. Dette apparat bruger en alginat kold polymer til at indstøbe vævsblokke og organer forud for skæring. Indstøbning af organer i agar forud for skæring i et apparat til frembringelse af snit med ens orientering og 20 tykkelse er også beskrevet i den stereologiske litteratur (CV Howard and MG Reed, Unbiased Stereology. Three-dimensional Measurement in Microscopy - Appendix A, BIOS Scientific Publishers, Oxford, 1998). I den samme beskrivelse er det vist hvordan apparatet benytter en bevægelig slæde med mulighed for variabel snit tykkelse.An apparatus for oriented cutting of tissue blocks and organs is described in PCT application No WO 00/37918. This apparatus uses an alginate cold polymer to embed tissue blocks and organs prior to cutting. Embedding organs in agar prior to cutting in an apparatus to produce sections of equal orientation and thickness are also described in the stereological literature (CV Howard and MG Reed, Unbiased Stereology. Three-Dimensional Measurement in Microscopy - Appendix A, BIOS Scientific Publishers, Oxford, 1998). In the same description, it is shown how the apparatus uses a movable carriage with the possibility of variable section thickness.
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Opfindelseshøjde i den aktuelle brugsmodelHeight of invention in the current utility model
Den aktuelle maskine adskiller sig fra den kendte teknik ved kombinationen af alginat kold polymer indstøbning og anvendelsen af en bevægelig slæde til skæring af snit med ens orientering og variabel tykkelse. Den nye maskine tillader orientering 30 af organet forud for skæring i den ikke hærdede alginat kold-polymejr. Skæremaskinen benytter to tætstillede parallelle plader til at styre vævs kniven. Disse 2 DK 2000 00326 U3 plader indeholder en rektangulær åbning der nøje passer til den alginat indstøbte vaevsblok på den bevægelige slæde. Dette forhindrer bevægelse af vævs og alginatblok under skærings proceduren.The present machine differs from the prior art in the combination of alginate cold polymer molding and the use of a movable slide for cutting sections of similar orientation and variable thickness. The new machine allows orientation of the member prior to cutting into the non-cured alginate cold polymer. The cutting machine uses two closely aligned parallel plates to guide the tissue knife. These 2 DK 2000 00326 U3 plates contain a rectangular opening that closely matches the alginate embedded tissue block on the movable slide. This prevents tissue and alginate block movement during the cutting procedure.
5 Beskrivelse af den aktuelle brugsmodel Vævsskæremaskinen har et indstøbningskammer (1), med en fure i bunden (2), der efter opfyldning med sammenblandet alginat og vand tillader organ indstøbning og orientering. Væggen af vævsindstøbningskammeret (1) er et rør i den foretrukne udformning; men kan også være ovalt eller en polygon. Røret passer over en niveau 10 forskydning i bundpladen (3), hvorved indstøbningskammeret (1) dannes. Bundpladen er udstyret med fire fødder (4) af f.eks. gummi eller latex. Bundpladen har også en skære-slæde (5) der passer over et styrings-spor (8). Skære-slæden har en fremføring bestående af en gevind skrue (10), som tillader variabel avancering af skære-slæden ved at dreje på skrue-knappen (9). Gevind-skruen (10) har en 15 tilhørende møtrik (12) med et indre gevind der vil fremføre møtrikken og dens kraftoverføringsarm (13) på gevind-skruen, når skrue-knappen (9) drejes. Dette resulterer i bevægelse af skære-slæden (5) på styrings-sporet (8). Skære-slæden og dens alginatblok støtter (6) kan passere gennem den rektangulære åbning i de to parallelle knivstyringsplader (15). De parallelle plader er fikseret til bundpladen med 20 et tyndt mellemrum (14) som netop tillader et tyndt barberblad at passere. Pladerne (15) er lavet af gennemsigtig plexiglas i den foretrukne udførelse; men kan også være lavet af andet materiale. Gennemsigtigheden hjælper til visuelt at styre kniven under skæringsproceduren. Skære-slæden (5) har en fure (7) i sin bundplade som modsvarer furen (2) i indstøbningskammerets bundplade.5 Description of the current utility model The tissue cutting machine has a molding chamber (1), with a groove at the bottom (2) which, after filling with mixed alginate and water, allows body molding and orientation. The wall of the tissue embedding chamber (1) is a tube of the preferred embodiment; but may also be oval or a polygon. The tube fits over a level 10 displacement in the base plate (3), thereby forming the embedding chamber (1). The base plate is equipped with four feet (4) of e.g. rubber or latex. The base plate also has a cutting slide (5) which fits over a guide groove (8). The cutting carriage has a feed consisting of a threaded screw (10) which allows variable cutting of the cutting carriage by turning the screw knob (9). The threaded screw (10) has an associated nut (12) with an internal thread which will propel the nut and its power transmission arm (13) onto the threaded screw as the screw knob (9) is turned. This results in movement of the cutting carriage (5) on the guide track (8). The cutting slide and its alginate block supports (6) can pass through the rectangular opening in the two parallel knife guide plates (15). The parallel plates are fixed to the base plate by a thin space (14) which permits a thin razor blade to pass. The plates (15) are made of transparent plexiglass in the preferred embodiment; but may also be made of other material. The transparency helps to visually guide the knife during the cutting procedure. The cutting carriage (5) has a groove (7) in its base plate which corresponds to the groove (2) in the base plate of the embedding chamber.
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Beskrivelse af indstøbnings- og skæringsprocessen Vævsblokken der skal skæres placeres i en blanding af alginat og vand i indstøbningskammeret (1). Alginaten hærder ved polymerisering over 2-5 minutter. Rørvæggen af indstøbningskammeret fjernes og alginat og vævsblokken kan fjernes. 30 Furen i bundpladen resulterer i en tilsvarende alginat-kam efter fjernelse af alginatblokken fra indstøbningskammeret (l). Alginat og vævsblokken kan nu 3 DK 2000 00326 U3 overføres til den bevægelige skære-slæde (5), der har halvmåne formede støtter (6) og en bundplade med en fure (7). Skære-slæden passer de ydre dimensioner af alginatblokken. Furen (7) sørger for at alginat og organblokken holder samme orientering i skære-slæden (5) som i indstøbningskammeret (1). Skrue-knappen (9) 5 på gevind-skruen drejes, hvilket resulterer i bevægelse af skære-slæden gennem kraftoverførsel via møtrikken (12) og dens arm eller tilhæftning (13). Alginat og vævsblokken flytter sig med skære-slæden (5) og passerer gennem den rektangulære åbning i de parallelle plader (15). Idet alginat og vævsblokken passserer de parallelle plader vil en serie af barberbladssnit gennem mellemrummet (13) resultere i en serie 10 af alginat og vævssnit med parallelle snitflader. Den resulterende snittykkelse er således variabel og afhængig af den rotatoriske fremføring af møtrikken (12) på gevind-skruen (10). Den distance som skære-slæden avanceres kan aflæses på mikrometer markeringer på skrue-knappen i den foretrukne udformning. Alternativt kan en mikrocator eller milimeter markeringer på styrings-sporet bruges til at aflæse 15 bevægelsen af skære-slæden.Description of the embedding and cutting process The tissue block to be cut is placed in a mixture of alginate and water in the embedding chamber (1). The alginate cures by polymerization over 2-5 minutes. The tube wall of the embedding chamber is removed and alginate and the tissue block can be removed. The groove in the bottom plate results in a corresponding alginate crest after removal of the alginate block from the embedding chamber (1). The alginate and the tissue block can now be transferred to the movable cutting carriage (5) having crescent shaped supports (6) and a bottom plate with a groove (7). The cutting sled fits the outer dimensions of the alginate block. The groove (7) ensures that the alginate and the organ block maintain the same orientation in the cutting carriage (5) as in the embedding chamber (1). The screw knob (9) 5 of the threaded screw is turned, resulting in movement of the cutting carriage through force transfer via the nut (12) and its arm or attachment (13). The alginate and the tissue block move with the cutting slide (5) and pass through the rectangular opening in the parallel plates (15). As the alginate and the tissue block pass the parallel plates, a series of razor blades through the gap (13) will result in a series 10 of alginate and tissue sections with parallel cut surfaces. The resulting cut thickness is thus variable and dependent on the rotatory feed of the nut (12) to the threaded screw (10). The distance advanced by the cutting carriage can be read on micrometer markings on the screw button in the preferred design. Alternatively, a microcator or millimeter markings on the guide track can be used to read the movement of the cutting carriage.
Priority Applications (1)
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DK200000326U DK200000326U3 (en) | 2000-11-02 | 2000-11-02 | An apparatus for cutting objects in uniform orientation and variable thickness |
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DK200000326U DK200000326U3 (en) | 2000-11-02 | 2000-11-02 | An apparatus for cutting objects in uniform orientation and variable thickness |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2002037080A1 (en) * | 2000-11-03 | 2002-05-10 | Histotech Aps | A device and a method for sectioning of tissue blocks |
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2000
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2002037080A1 (en) * | 2000-11-03 | 2002-05-10 | Histotech Aps | A device and a method for sectioning of tissue blocks |
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