DK1781807T3 - Fluorescence-based kinetic measurement of transglutaminase activity - Google Patents

Fluorescence-based kinetic measurement of transglutaminase activity

Info

Publication number
DK1781807T3
DK1781807T3 DK05768798T DK05768798T DK1781807T3 DK 1781807 T3 DK1781807 T3 DK 1781807T3 DK 05768798 T DK05768798 T DK 05768798T DK 05768798 T DK05768798 T DK 05768798T DK 1781807 T3 DK1781807 T3 DK 1781807T3
Authority
DK
Denmark
Prior art keywords
fluorescence
transglutaminase activity
kinetic measurement
transglutaminase
based kinetic
Prior art date
Application number
DK05768798T
Other languages
Danish (da)
Inventor
Ralf Pasternack
Kai Oertel
Original Assignee
Zedira Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP04019090A external-priority patent/EP1626095A1/en
Application filed by Zedira Gmbh filed Critical Zedira Gmbh
Application granted granted Critical
Publication of DK1781807T3 publication Critical patent/DK1781807T3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/52—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving transaminase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/56—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

Abstract

The invention relates to a method for the fluorescence-based kinetic measurement of transglutaminase activity. Said method is characterized in that the transglutaminase is reacted with a modified peptide substrate in which a fluorophore is bonded directly to the N terminal of the peptide and a glutaminic acid radical carries a fluorescence quencher in position 5, said fluorescence quencher being bonded to position 5 via a spacer containing an amine function so as to form an amide bond, such that the fluorescence quencher is separated as a result of the transglutaminase activity, and the resulting fluorescence of the remaining molecule is measured.
DK05768798T 2004-08-11 2005-08-05 Fluorescence-based kinetic measurement of transglutaminase activity DK1781807T3 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP04019090A EP1626095A1 (en) 2004-08-11 2004-08-11 Fluorescence-based kinetic determination of transglutaminase activity
US60337404P 2004-08-20 2004-08-20
PCT/EP2005/008532 WO2006018164A2 (en) 2004-08-11 2005-08-05 Fluorescence-based kinetic determination of transglutaminase activity

Publications (1)

Publication Number Publication Date
DK1781807T3 true DK1781807T3 (en) 2009-08-31

Family

ID=35482324

Family Applications (1)

Application Number Title Priority Date Filing Date
DK05768798T DK1781807T3 (en) 2004-08-11 2005-08-05 Fluorescence-based kinetic measurement of transglutaminase activity

Country Status (5)

Country Link
EP (1) EP1781807B1 (en)
AT (1) ATE416259T1 (en)
DE (1) DE502005006161D1 (en)
DK (1) DK1781807T3 (en)
WO (1) WO2006018164A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7464931B2 (en) 2005-03-31 2008-12-16 Heidelberger Druckmaschinen Ag Apparatus for positioning a trailing edge of sheets
KR100862318B1 (en) * 2007-03-19 2008-10-13 강원대학교산학협력단 Array-based transglutaminase activity assay chip and transglutaminase activity assay method
WO2010046468A1 (en) * 2008-10-24 2010-04-29 Novo Nordisk Health Care Ag Method of quality control testing a factor xiii containing sample
KR101008218B1 (en) 2008-11-14 2011-01-17 강원대학교산학협력단 In situ transglutaminase activity assay method
EP2348038A1 (en) 2010-01-22 2011-07-27 Philipps-Universität Marburg Method for determining the activity of transglutaminase factor XIIIa
EP2687511A1 (en) 2012-07-17 2014-01-22 Zedira GmbH Pyridinone derivatives as tissue transglutaminase inhibitors
WO2015171693A1 (en) * 2014-05-06 2015-11-12 Virginia Commonwealth University A new target for firmicutes and related bacteria: the prp protease
EP3342779A1 (en) 2016-12-27 2018-07-04 Zedira GmbH Inhibitors of transglutaminases

Also Published As

Publication number Publication date
DE502005006161D1 (en) 2009-01-15
EP1781807B1 (en) 2008-12-03
WO2006018164A2 (en) 2006-02-23
EP1781807A2 (en) 2007-05-09
WO2006018164A3 (en) 2006-05-26
ATE416259T1 (en) 2008-12-15

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