DK1633876T3 - Fremgangsmåde til fremstilling af insulin i planter - Google Patents

Fremgangsmåde til fremstilling af insulin i planter

Info

Publication number
DK1633876T3
DK1633876T3 DK04737835T DK04737835T DK1633876T3 DK 1633876 T3 DK1633876 T3 DK 1633876T3 DK 04737835 T DK04737835 T DK 04737835T DK 04737835 T DK04737835 T DK 04737835T DK 1633876 T3 DK1633876 T3 DK 1633876T3
Authority
DK
Denmark
Prior art keywords
plants
producing insulin
insulin
producing
Prior art date
Application number
DK04737835T
Other languages
English (en)
Inventor
Maurice M Moloney
Joseph Boothe
Richard Keon
Cory Nykiforuk
Rooijen Gijs Van
Original Assignee
Sembiosys Genetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sembiosys Genetics Inc filed Critical Sembiosys Genetics Inc
Application granted granted Critical
Publication of DK1633876T3 publication Critical patent/DK1633876T3/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575Hormones
    • C07K14/62Insulins
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DK04737835T 2003-06-17 2004-06-17 Fremgangsmåde til fremstilling af insulin i planter DK1633876T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US47881803P 2003-06-17 2003-06-17
US54953904P 2004-03-04 2004-03-04
PCT/CA2004/000896 WO2004111244A2 (en) 2003-06-17 2004-06-17 Methods for the production of insulin in plants

Publications (1)

Publication Number Publication Date
DK1633876T3 true DK1633876T3 (da) 2008-12-08

Family

ID=33555509

Family Applications (1)

Application Number Title Priority Date Filing Date
DK04737835T DK1633876T3 (da) 2003-06-17 2004-06-17 Fremgangsmåde til fremstilling af insulin i planter

Country Status (24)

Country Link
US (2) US7547821B2 (da)
EP (2) EP1633876B1 (da)
JP (2) JP4864701B2 (da)
KR (1) KR101216762B1 (da)
CN (1) CN1836047B (da)
AR (1) AR044803A1 (da)
AT (1) ATE405660T1 (da)
AU (1) AU2004247762B2 (da)
BR (1) BRPI0411479A (da)
CA (1) CA2528741A1 (da)
CY (1) CY1108477T1 (da)
DE (1) DE602004015980D1 (da)
DK (1) DK1633876T3 (da)
EA (1) EA014887B1 (da)
ES (1) ES2313018T3 (da)
HK (1) HK1088037A1 (da)
IL (1) IL172469A (da)
MX (1) MXPA05013723A (da)
NO (1) NO20055885L (da)
NZ (1) NZ544522A (da)
PL (1) PL1633876T3 (da)
PT (1) PT1633876E (da)
TW (1) TWI345587B (da)
WO (1) WO2004111244A2 (da)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003213815A1 (en) * 2002-03-08 2003-09-22 Prodigene, Inc. Production of insulin and insulin-like proteins in plants
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
EP1563075A4 (en) * 2002-11-12 2006-09-06 Ckd Bio Corp HUMANINSULIN EXPRESSIVE PLASMIDE AND METHOD FOR THE PRODUCTION OF HUMANINSULIN THEREWITH
US20040173547A1 (en) * 2003-03-06 2004-09-09 Roth Michael Carl Bolt on attachment to facilitate hanging of pneumatic tool
AR044803A1 (es) * 2003-06-17 2005-10-05 Sembiosys Genetics Inc Metodos para la produccion de insulina
CA2528845A1 (en) * 2003-06-20 2004-12-29 Sembiosys Genetics Inc. Modified oleosins
TW200526778A (en) * 2003-11-14 2005-08-16 Sembiosys Genetics Inc Methods for the production of apolipoproteins in transgenic plants
WO2006014899A2 (en) 2004-07-26 2006-02-09 Dow Global Technologies Inc. Process for improved protein expression by strain engineering
AR053477A1 (es) 2005-05-23 2007-05-09 Arcadia Biosciences Inc Cartamo con acido gamma - linolenico elevado
US20080274143A1 (en) * 2005-05-27 2008-11-06 Henry Daniell Chloroplasts Engineering to Express Pharmaceutical Proteins
WO2007005928A2 (en) * 2005-07-01 2007-01-11 Cornell Research Foundation Oleosin genes and promoters from coffee
WO2007081824A2 (en) * 2006-01-06 2007-07-19 Case Western Reserve University Fibrillation resistant proteins
WO2008028119A2 (en) 2006-08-31 2008-03-06 Monsanto Technology Llc. Methods for rapidly transforming monocots
EP2074140B8 (en) 2006-10-04 2015-10-28 Case Western Reserve University Fibrillation-resistant insulin and insulin analogues
AU2008245696B2 (en) 2007-04-27 2013-11-07 Pelican Technology Holdings, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
AU2009226910B2 (en) 2008-03-18 2014-02-06 Novo Nordisk A/S Protease stabilized, acylated insulin analogues
US8993516B2 (en) * 2008-04-14 2015-03-31 Case Western Reserve University Meal-time insulin analogues of enhanced stability
BRPI0911571A2 (pt) * 2008-04-22 2018-04-03 Univ Case Western Reserve método para tratar um mamífero, análogo de insulina, ácido nucléico e célula hospedeira
PL219335B1 (pl) * 2008-07-04 2015-04-30 Inst Biotechnologii I Antybiotyków Pochodna insuliny lub jej farmaceutycznie dopuszczalna sól, jej zastosowanie oraz zawierająca ją kompozycja farmaceutyczna
US9200053B2 (en) 2008-07-31 2015-12-01 Case Western Reserve University Insulin analogues containing penta-fluoro-Phenylalanine at position B24
KR20120129875A (ko) 2008-07-31 2012-11-28 케이스 웨스턴 리저브 유니버시티 염소화 아미노산을 갖는 인슐린 유사체
CN101736029B (zh) * 2008-11-21 2013-01-02 复旦大学 一种利用植物油体蛋白表达***生产人***-i的方法
US8399407B2 (en) * 2009-09-17 2013-03-19 Case Western Reserve University Non-standard insulin analogues
MY189079A (en) 2009-12-11 2022-01-25 Univ Case Western Reserve Insulin analogues with chlorinated amino acids
CN102268451B (zh) * 2010-06-01 2014-03-12 安胜军 一种含有人胰岛素基因的表达载体及其构建方法与应用
BR112012032126A2 (pt) 2010-06-16 2017-10-17 Futuragene Israel Ltd Empresa Isralense polinucleotídeo isolado, estrutura de ácido nucléico, sistema de estrutura de ácido nucléico, polipeptídeo isolado, planta, composição inseticida, e método para controlar ou exterminar um inseto
US20130164288A1 (en) 2010-09-07 2013-06-27 Protalix Ltd. Readthrough acetylcholinesterase (ache-r) for treating or preventing parkinson's disease
WO2013059213A1 (en) * 2011-10-18 2013-04-25 Amidebio, Llc Chemically and thermodynamically stable insulin analogues and improved methods for their production
US9352456B2 (en) 2011-10-26 2016-05-31 Black & Decker Inc. Power tool with force sensing electronic clutch
AU2013247047A1 (en) 2012-04-11 2014-10-23 Novo Nordisk A/S Insulin formulations
WO2014172488A2 (en) 2013-04-17 2014-10-23 AmideBio LLC Chemically and thermodynamically stable insulin analogues and improved methods for their production
KR101625746B1 (ko) 2013-09-23 2016-05-31 순천대학교 산학협력단 프로미니인슐린 재조합 단백질이 발현된 형질전환 식물 및 이의 제조방법
KR102569743B1 (ko) 2014-10-06 2023-08-23 케이스 웨스턴 리저브 유니버시티 이상 단일 사슬 인슐린 유사체
BR112019011761A2 (pt) 2016-12-16 2019-11-05 Novo Nordisk As composições farmacêuticas contendo insulina
KR102642178B1 (ko) * 2017-08-08 2024-02-29 중앙대학교 산학협력단 돌연변이에 의해, 혈중 반감기 및 공수병 바이러스 중화 효능이 증대된 공수병 바이러스 중화 항체
BR112020015238A2 (pt) * 2018-02-09 2020-12-29 Jiangsu Hengrui Medicine Co., Ltd. Gene precursor otimizado por códon e gene de peptídeo sinal de análogo de insulina humana
EP3956355A4 (en) * 2019-04-19 2023-01-18 The Trustees of Indiana University STABILIZATION OF PRANDIAL OR BASAL INSULIN ANALOGS BY AN INTERNAL DISELENIDE BRIDGE
US20230018324A1 (en) * 2019-12-06 2023-01-19 Dr. Mary Morris & Associates, Llc Methods and compositions for the treatment and prevention of type 1 diabetes
IL299545A (en) * 2020-07-03 2023-02-01 Kriya Therapeutics Inc Different insulin and glucokinase nucleic acids for the treatment of diabetes
EP4190905A1 (en) 2021-12-01 2023-06-07 Core Biogenesis Method for the purification of recombinant proteins using oil body proteins

Family Cites Families (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3971856A (en) 1975-03-03 1976-07-27 Archer Daniels Midland Company Process for preparing soy protein concentrate
US6174724B1 (en) 1983-01-17 2001-01-16 Monsanto Company Chimeric genes suitable for expression in plant cells
NL8300698A (nl) 1983-02-24 1984-09-17 Univ Leiden Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; agrobacterium tumefaciens bacterien en werkwijze voor het produceren daarvan; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten.
US5504200A (en) 1983-04-15 1996-04-02 Mycogen Plant Science, Inc. Plant gene expression
DK58285D0 (da) 1984-05-30 1985-02-08 Novo Industri As Peptider samt fremstilling og anvendelse deraf
US5008241A (en) 1985-03-12 1991-04-16 Novo Nordisk A/S Novel insulin peptides
US4956282A (en) 1985-07-29 1990-09-11 Calgene, Inc. Mammalian peptide expression in plant cells
ATE85360T1 (de) 1985-08-07 1993-02-15 Monsanto Co Glyphosat resistente pflanzen.
US5273894A (en) 1986-08-23 1993-12-28 Hoechst Aktiengesellschaft Phosphinothricin-resistance gene, and its use
US5268463A (en) 1986-11-11 1993-12-07 Jefferson Richard A Plant promoter α-glucuronidase gene construct
EP0513849B1 (en) 1987-05-20 1998-08-19 Novartis AG Method of preparing transgenic Zea mays plants regenerated from protoplasts or protoplast-derived cells
DK336188D0 (da) 1988-06-20 1988-06-20 Nordisk Gentofte Propeptider
CA1341467C (en) 1988-07-29 2004-12-07 John C. Rogers Producing commercially valuable polypeptides with genetically transformed endosperm tissue
HUT56857A (en) 1988-12-23 1991-10-28 Novo Nordisk As Human insulin analogues
EP0392225B1 (en) 1989-03-24 2003-05-28 Syngenta Participations AG Disease-resistant transgenic plants
NL8901932A (nl) 1989-07-26 1991-02-18 Mogen Int Produktie van heterologe eiwitten in planten of plantecellen.
US4940935A (en) 1989-08-28 1990-07-10 Ried Ashman Manufacturing Automatic SMD tester
US5202422A (en) 1989-10-27 1993-04-13 The Scripps Research Institute Compositions containing plant-produced glycopolypeptide multimers, multimeric proteins and method of their use
US5959177A (en) 1989-10-27 1999-09-28 The Scripps Research Institute Transgenic plants expressing assembled secretory antibodies
KR910014790A (ko) 1990-01-10 1991-08-31 한태희 랩탑 컴퓨터의 기능선택방법 및 인터페이스회로
WO1991013993A1 (en) * 1990-03-05 1991-09-19 The Upjohn Company Protein expression via seed specific regulatory sequences
US5650554A (en) 1991-02-22 1997-07-22 Sembiosys Genetics Inc. Oil-body proteins as carriers of high-value peptides in plants
UA48104C2 (uk) 1991-10-04 2002-08-15 Новартіс Аг Фрагмент днк, який містить послідовність,що кодує інсектицидний протеїн, оптимізовану для кукурудзи,фрагмент днк, який забезпечує направлену бажану для серцевини стебла експресію зв'язаного з нею структурного гена в рослині, фрагмент днк, який забезпечує специфічну для пилку експресію зв`язаного з нею структурного гена в рослині, рекомбінантна молекула днк, спосіб одержання оптимізованої для кукурудзи кодуючої послідовності інсектицидного протеїну, спосіб захисту рослин кукурудзи щонайменше від однієї комахи-шкідника
DE69332538T2 (de) 1992-04-02 2003-11-06 Sembiosys Genetics Inc Cis elemente des ölkörperproteins als regulationische signale
DK0677112T3 (da) * 1992-12-28 2003-07-21 Stichting Scheikundig Onderzoe Fremgangsmåde til at opnå transgene planter med et modificeret fructanmønster
US5474978A (en) 1994-06-16 1995-12-12 Eli Lilly And Company Insulin analog formulations
US5461031A (en) 1994-06-16 1995-10-24 Eli Lilly And Company Monomeric insulin analog formulations
US5633437A (en) 1994-10-11 1997-05-27 Sandoz Ltd. Gene exhibiting resistance to acetolactate synthase inhibitor herbicides
US6040496A (en) 1995-06-30 2000-03-21 Novartis Finance Corporation Use of translationally altered RNA to confer resistance to maize dwarf mosaic virus and other monocotyledonous plant viruses
US5577474A (en) * 1995-11-29 1996-11-26 General Motors Corporation Torque estimation for engine speed control
CA2246242A1 (en) * 1996-02-08 1997-08-14 Institut Fur Pflanzengenetik Und Kulturpflanzenforschung Cassettes for the expression of storable proteins in plants
US5856452A (en) * 1996-12-16 1999-01-05 Sembiosys Genetics Inc. Oil bodies and associated proteins as affinity matrices
ES2273414T3 (es) 1997-04-25 2007-05-01 Sembiosys Genetics Inc. Procedimiento para el corte de proteinas de fusion.
ES2276475T5 (es) * 1997-09-30 2014-07-11 The Regents Of The University Of California Producción de proteínas en semillas de plantas
AR016229A1 (es) * 1998-04-27 2001-06-20 Demegen Inc Genes utiles para incrementar el contenido proteico de plantas, proteinas codificadas por dichos genes, celulas de plantas transfectadas con dichos genesy metodos para incrementar el contenido proteico o no proteico de plantas
US6429357B1 (en) 1999-05-14 2002-08-06 Dekalb Genetics Corp. Rice actin 2 promoter and intron and methods for use thereof
JP2003525030A (ja) 1999-08-27 2003-08-26 セムバイオシス ジェネティックス インコーポレイテッド 亜麻種子特異的プロモーター
US6521738B2 (en) 1999-12-29 2003-02-18 Novo Nordisk A/S Method for making insulin precursors and insulin precursor analogs
ATE510916T1 (de) 2000-03-01 2011-06-15 Univ Auburn Plastidtransformationsvektoren zur expression von proinsulin, das an die cholera toxin b- untereinheit fusioniert ist, in pflanzen
CN1295129A (zh) * 2000-08-25 2001-05-16 林忠平 利用转基因番茄生产人胰岛素的方法
TWI317736B (en) * 2000-12-19 2009-12-01 Sembiosys Genetics Inc Methods for the production of redox proteins and heteromultimeric-protein-complexes, and related compositions related applications
EP1261121A3 (en) 2001-05-22 2004-02-04 Powerdsine Limited Power distribution with digital current control
AU2003213815A1 (en) * 2002-03-08 2003-09-22 Prodigene, Inc. Production of insulin and insulin-like proteins in plants
CN1382734A (zh) * 2002-03-15 2002-12-04 林忠平 利用转基因生菜生产人胰岛素的方法
AR044803A1 (es) * 2003-06-17 2005-10-05 Sembiosys Genetics Inc Metodos para la produccion de insulina

Also Published As

Publication number Publication date
BRPI0411479A (pt) 2006-07-25
US20090282583A1 (en) 2009-11-12
AU2004247762B2 (en) 2010-05-27
IL172469A0 (en) 2006-04-10
NO20055885L (no) 2006-01-17
EP1633876A2 (en) 2006-03-15
TW200510529A (en) 2005-03-16
JP2011200251A (ja) 2011-10-13
EA014887B1 (ru) 2011-02-28
AR044803A1 (es) 2005-10-05
KR101216762B1 (ko) 2013-01-02
ES2313018T3 (es) 2009-03-01
IL172469A (en) 2011-08-31
PT1633876E (pt) 2008-11-03
NZ544522A (en) 2008-03-28
KR20060052705A (ko) 2006-05-19
CY1108477T1 (el) 2014-04-09
EP1959017A1 (en) 2008-08-20
CN1836047A (zh) 2006-09-20
WO2004111244A3 (en) 2005-02-03
ATE405660T1 (de) 2008-09-15
CA2528741A1 (en) 2004-12-23
JP4864701B2 (ja) 2012-02-01
TWI345587B (en) 2011-07-21
JP2007523611A (ja) 2007-08-23
US7547821B2 (en) 2009-06-16
PL1633876T3 (pl) 2009-01-30
AU2004247762A1 (en) 2004-12-23
US20050039235A1 (en) 2005-02-17
EP1633876B1 (en) 2008-08-20
MXPA05013723A (es) 2006-03-13
DE602004015980D1 (de) 2008-10-02
HK1088037A1 (en) 2006-10-27
CN1836047B (zh) 2010-12-22
EA200600050A1 (ru) 2006-06-30
WO2004111244A2 (en) 2004-12-23

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