DK1481057T3 - System og fremgangsmåde til fremstilling af rekombinante glycosylerede proteiner i en prokaryot vært - Google Patents
System og fremgangsmåde til fremstilling af rekombinante glycosylerede proteiner i en prokaryot værtInfo
- Publication number
- DK1481057T3 DK1481057T3 DK03702276T DK03702276T DK1481057T3 DK 1481057 T3 DK1481057 T3 DK 1481057T3 DK 03702276 T DK03702276 T DK 03702276T DK 03702276 T DK03702276 T DK 03702276T DK 1481057 T3 DK1481057 T3 DK 1481057T3
- Authority
- DK
- Denmark
- Prior art keywords
- prokaryotic host
- producing recombinant
- glycosylated proteins
- recombinant glycosylated
- oligosaccharide
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/005—Glycopeptides, glycoproteins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/02—Bacterial antigens
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/54—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
- A61K47/549—Sugars, nucleosides, nucleotides or nucleic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
- C07K16/1203—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-negative bacteria
- C07K16/121—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-negative bacteria from Helicobacter (Campylobacter) (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
- C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Mycology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CH3942002 | 2002-03-07 | ||
US36465502P | 2002-03-14 | 2002-03-14 |
Publications (1)
Publication Number | Publication Date |
---|---|
DK1481057T3 true DK1481057T3 (da) | 2006-05-15 |
Family
ID=27789602
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK03702276T DK1481057T3 (da) | 2002-03-07 | 2003-03-05 | System og fremgangsmåde til fremstilling af rekombinante glycosylerede proteiner i en prokaryot vært |
Country Status (12)
Country | Link |
---|---|
US (3) | US20050287628A1 (da) |
EP (1) | EP1481057B1 (da) |
KR (1) | KR20090110951A (da) |
AT (1) | ATE317897T1 (da) |
AU (1) | AU2003205502C1 (da) |
CA (1) | CA2477794C (da) |
DE (1) | DE60303591T2 (da) |
DK (1) | DK1481057T3 (da) |
ES (1) | ES2258214T3 (da) |
IL (3) | IL163806A0 (da) |
PT (1) | PT1481057E (da) |
WO (1) | WO2003074687A1 (da) |
Families Citing this family (40)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1481057B1 (en) * | 2002-03-07 | 2006-02-15 | Eidgenössische Technische Hochschule Zürich | System and method for the production of recombinant glycosylated proteins in a prokaryotic host |
ES2703061T3 (es) | 2005-05-11 | 2019-03-06 | Eth Zuerich | Proteínas N-glicosiladas recombinantes procedentes de células procariotas |
AU2011218682B2 (en) * | 2005-05-11 | 2013-11-21 | Eth Zurich | Recombinant N-glycosylated proteins from procaryotic cells |
EP2240595B2 (en) | 2008-01-03 | 2019-07-24 | Cornell Research Foundation, Inc. | Glycosylated protein expression in prokaryotes |
KR20100123730A (ko) | 2008-02-20 | 2010-11-24 | 글리코박신 아게 | 원핵생물 세포로부터의 n-당화된 재조합 단백질로부터 제조된 바이오컨쥬게이트 |
CN102724997B (zh) | 2009-11-19 | 2016-09-21 | 格林考瓦因有限公司 | 在原核细胞中产生免疫原性多糖的生物合成*** |
ES2657588T3 (es) | 2010-05-06 | 2018-03-06 | Glaxosmithkline Biologicals Sa | Vacunas de bioconjugados de bacterias grampositivas capsulares |
AU2012297533B2 (en) | 2011-08-12 | 2016-02-25 | Instituto De Investigaciones Biotecnologicas-Instituto Tecnologico De Chascomus Consejo De Investigaciones Cientificas Y Tecnicas, Universidad Nacional De San Martin | Method of diagnosing bacterial infections using bacterial glycoproteins |
US20140336366A1 (en) | 2011-09-06 | 2014-11-13 | Glycovaxyn Ag | Bioconjugate vaccines made in prokaryotic cells |
WO2013067523A1 (en) * | 2011-11-04 | 2013-05-10 | Cornell University | A prokaryote-based cell-free system for the synthesis of glycoproteins |
EA201590705A1 (ru) | 2012-11-07 | 2016-05-31 | Гликоваксин Аг | ПОЛУЧЕНИЕ РЕКОМБИНАНТНОЙ ВАКЦИНЫ В E.coli ПОСРЕДСТВОМ ЭНЗИМАТИЧЕСКОЙ КОНЪЮГАЦИИ |
ES2718051T3 (es) | 2013-01-17 | 2019-06-27 | Arsanis Biosciences Gmbh | Antígeno específico de E. coli MDR |
BR112015025288B1 (pt) * | 2013-04-05 | 2023-02-23 | The Governors Of The University Of Alberta | Composição de vacina para uso na vacinação de galinhas contra campylobacter |
CN106535927B (zh) | 2014-02-24 | 2019-09-20 | 葛兰素史密丝克莱恩生物有限公司 | 新颖多糖及其用途 |
SI3131577T1 (sl) | 2014-04-17 | 2020-08-31 | Glaxosmithkline Biologicals S.A. | Modificirane gostiljske celice in uporabe le-teh |
BE1022565B1 (fr) | 2014-08-08 | 2016-06-03 | Glycovaxyn Ag | Cellules hotes modifiees pour une utilisation dans la production de bioconjugues |
US9616114B1 (en) | 2014-09-18 | 2017-04-11 | David Gordon Bermudes | Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity |
TWI715617B (zh) | 2015-08-24 | 2021-01-11 | 比利時商葛蘭素史密斯克藍生物品公司 | 對抗腸道外病原性大腸桿菌之免疫保護之方法及組合物 |
GB201610599D0 (en) | 2016-06-17 | 2016-08-03 | Glaxosmithkline Biologicals Sa | Immunogenic Composition |
AR109621A1 (es) | 2016-10-24 | 2018-12-26 | Janssen Pharmaceuticals Inc | Formulaciones de vacunas contra glucoconjugados de expec |
US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
US11180535B1 (en) | 2016-12-07 | 2021-11-23 | David Gordon Bermudes | Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria |
GB201704117D0 (en) * | 2017-03-15 | 2017-04-26 | London School Hygiene & Tropical Medicine | Oligosaccharyl Transferase polypeptide |
WO2019035916A1 (en) | 2017-08-15 | 2019-02-21 | Northwestern University | DESIGN OF PROTEIN GLYCOSYLATION SITES BY RAPID EXPRESSION AND CHARACTERIZATION OF N-GLYCOSYLTRANSFERASES |
GB201721582D0 (en) | 2017-12-21 | 2018-02-07 | Glaxosmithkline Biologicals Sa | S aureus antigens and immunogenic compositions |
GB201721576D0 (en) | 2017-12-21 | 2018-02-07 | Glaxosmithkline Biologicals Sa | Hla antigens and glycoconjugates thereof |
US11530432B2 (en) | 2018-03-19 | 2022-12-20 | Northwestern University | Compositions and methods for rapid in vitro synthesis of bioconjugate vaccines in vitro via production and N-glycosylation of protein carriers in detoxified prokaryotic cell lysates |
US11725224B2 (en) | 2018-04-16 | 2023-08-15 | Northwestern University | Methods for co-activating in vitro non-standard amino acid (nsAA) incorporation and glycosylation in crude cell lysates |
US20210395728A1 (en) * | 2018-08-29 | 2021-12-23 | Council Of Scientific & Industrial Research | Recombinant microbial system for directed evolution of glycocins and method of preparation thereof |
CN113227125A (zh) | 2018-12-12 | 2021-08-06 | 葛兰素史密丝克莱恩生物有限公司 | 用于o-连接的糖基化的修饰的载体蛋白 |
US20220267821A1 (en) * | 2019-01-11 | 2022-08-25 | Northwestern University | Bioconjugate vaccines' synthesis in prokaryotic cell lysates |
AR118389A1 (es) | 2019-03-18 | 2021-09-29 | Janssen Pharmaceuticals Inc | Métodos de producción de bioconjugados de polisacáridos de e. coli o-antígeno, sus composiciones y métodos de utilización |
JOP20210256A1 (ar) | 2019-03-18 | 2023-01-30 | Janssen Pharmaceuticals Inc | متقارن حيوي لعديدات سكاريد المستضدات-o للإشريكية القولونية، طرائق إنتاجه، وطرائق استخدامه |
EP3757217A1 (en) | 2019-06-27 | 2020-12-30 | GlaxoSmithKline Biologicals S.A. | Methods for protein purification |
EP3770269A1 (en) | 2019-07-23 | 2021-01-27 | GlaxoSmithKline Biologicals S.A. | Quantification of bioconjugate glycosylation |
EP3777884A1 (en) | 2019-08-15 | 2021-02-17 | GlaxoSmithKline Biologicals S.A. | Immunogenic composition |
BR112022024294A2 (pt) | 2020-06-18 | 2023-02-28 | Glaxosmithkline Biologicals Sa | Bioconjugado shigella-tetravalente (shigella4v)". |
EP4171624A1 (en) | 2020-06-25 | 2023-05-03 | GlaxoSmithKline Biologicals S.A. | Modified exotoxin a proteins |
EP4213870A1 (en) | 2020-09-17 | 2023-07-26 | Janssen Pharmaceuticals, Inc. | Multivalent vaccine compositions and uses thereof |
WO2023118033A1 (en) | 2021-12-22 | 2023-06-29 | Glaxosmithkline Biologicals Sa | Vaccine |
Family Cites Families (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0170204B1 (de) | 1984-08-01 | 1991-09-25 | BOEHRINGER INGELHEIM INTERNATIONAL GmbH | Neue genetische Sequenzen, die durch sie codierten Interferon-Peptide vom Typ I und diese sie produzierende Organismen |
US5643758A (en) * | 1987-03-10 | 1997-07-01 | New England Biolabs, Inc. | Production and purification of a protein fused to a binding protein |
EP0726318A1 (en) | 1993-05-14 | 1996-08-14 | The Upjohn Company | An acceptor polypeptide for an N-acetylgalactosaminyltransferase |
US6503744B1 (en) | 1999-02-01 | 2003-01-07 | National Research Council Of Canada | Campylobacter glycosyltransferases for biosynthesis of gangliosides and ganglioside mimics |
EP1399538A2 (en) * | 1999-03-02 | 2004-03-24 | Human Genome Sciences, Inc. | Engineering intracellular sialylation pathways |
MXPA02011016A (es) * | 2000-05-12 | 2004-03-16 | Neose Technologies Inc | Glicopeptidos recombinantes de glucolisacion in vitro. |
JP4092194B2 (ja) | 2000-06-30 | 2008-05-28 | フランダース インターユニバーシティ インスティテュート フォア バイオテクノロジー (ヴィーアイビー) | ピキアパストリス(PichiaPastoris)におけるタンパク質糖鎖修飾 |
EP1481057B1 (en) | 2002-03-07 | 2006-02-15 | Eidgenössische Technische Hochschule Zürich | System and method for the production of recombinant glycosylated proteins in a prokaryotic host |
KR100938026B1 (ko) | 2002-03-07 | 2010-01-21 | 아이드게노쉬쉐 테흐니쉐 호흐슐레 쥬리히 | 원핵 숙주에서 재조합 글리코실화 단백질의 생산방법 및생산계 |
US7598354B2 (en) | 2002-08-01 | 2009-10-06 | National Research Council Of Canada | Campylobacter glycans and glycopeptides |
WO2005116063A1 (en) | 2004-05-24 | 2005-12-08 | The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Live, oral vaccine for protection against shigella dysenteriae serotype 1 |
ES2703061T3 (es) | 2005-05-11 | 2019-03-06 | Eth Zuerich | Proteínas N-glicosiladas recombinantes procedentes de células procariotas |
KR20100123730A (ko) * | 2008-02-20 | 2010-11-24 | 글리코박신 아게 | 원핵생물 세포로부터의 n-당화된 재조합 단백질로부터 제조된 바이오컨쥬게이트 |
CN102724997B (zh) * | 2009-11-19 | 2016-09-21 | 格林考瓦因有限公司 | 在原核细胞中产生免疫原性多糖的生物合成*** |
ES2657588T3 (es) * | 2010-05-06 | 2018-03-06 | Glaxosmithkline Biologicals Sa | Vacunas de bioconjugados de bacterias grampositivas capsulares |
-
2003
- 2003-03-05 EP EP03702276A patent/EP1481057B1/en not_active Expired - Lifetime
- 2003-03-05 KR KR1020097020064A patent/KR20090110951A/ko not_active Application Discontinuation
- 2003-03-05 IL IL16380603A patent/IL163806A0/xx unknown
- 2003-03-05 AU AU2003205502A patent/AU2003205502C1/en not_active Expired
- 2003-03-05 US US10/506,917 patent/US20050287628A1/en not_active Abandoned
- 2003-03-05 AT AT03702276T patent/ATE317897T1/de active
- 2003-03-05 WO PCT/CH2003/000153 patent/WO2003074687A1/en active Search and Examination
- 2003-03-05 ES ES03702276T patent/ES2258214T3/es not_active Expired - Lifetime
- 2003-03-05 DK DK03702276T patent/DK1481057T3/da active
- 2003-03-05 CA CA2477794A patent/CA2477794C/en not_active Expired - Lifetime
- 2003-03-05 PT PT03702276T patent/PT1481057E/pt unknown
- 2003-03-05 DE DE60303591T patent/DE60303591T2/de not_active Expired - Lifetime
-
2004
- 2004-08-30 IL IL163806A patent/IL163806A/en active IP Right Grant
-
2008
- 2008-07-21 US US12/219,383 patent/US8703471B2/en not_active Expired - Lifetime
-
2012
- 2012-06-12 IL IL220322A patent/IL220322A0/en active IP Right Grant
-
2014
- 2014-02-25 US US14/189,673 patent/US20140335127A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
EP1481057B1 (en) | 2006-02-15 |
US8703471B2 (en) | 2014-04-22 |
AU2003205502A1 (en) | 2003-09-16 |
IL163806A (en) | 2012-08-30 |
US20140335127A1 (en) | 2014-11-13 |
DE60303591D1 (de) | 2006-04-20 |
US20090074798A1 (en) | 2009-03-19 |
IL220322A0 (en) | 2012-07-31 |
WO2003074687A1 (en) | 2003-09-12 |
KR20090110951A (ko) | 2009-10-23 |
ES2258214T3 (es) | 2006-08-16 |
AU2003205502C1 (en) | 2011-08-25 |
ATE317897T1 (de) | 2006-03-15 |
EP1481057A1 (en) | 2004-12-01 |
US20050287628A1 (en) | 2005-12-29 |
IL163806A0 (en) | 2005-12-18 |
CA2477794C (en) | 2013-08-20 |
DE60303591T2 (de) | 2007-03-01 |
CA2477794A1 (en) | 2003-09-12 |
PT1481057E (pt) | 2006-05-31 |
AU2003205502B2 (en) | 2008-01-31 |
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