DK146512B - PROCEDURE FOR CULTIVATING THE BASIDIOMYCETE FUNGI CORIOLUS VERSICOLOR (FR.) QUEL. - Google Patents

PROCEDURE FOR CULTIVATING THE BASIDIOMYCETE FUNGI CORIOLUS VERSICOLOR (FR.) QUEL. Download PDF

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DK146512B
DK146512B DK385077AA DK385077A DK146512B DK 146512 B DK146512 B DK 146512B DK 385077A A DK385077A A DK 385077AA DK 385077 A DK385077 A DK 385077A DK 146512 B DK146512 B DK 146512B
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glucose
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coriolus versicolor
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Chikao Yoshikumi
Takao Furusho
Kenichi Matsunaga
Noriyuki Toyoda
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Kureha Chemical Ind Co Ltd
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    • A—HUMAN NECESSITIES
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    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
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    • A61K36/07—Basidiomycota, e.g. Cryptococcus
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    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
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    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi

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Description

(19) DANMARK (W)(19) DENMARK (W)

φ dg FREMLÆGGELSESSKRIFT (n) 146512 Bφ dg PUBLICATION MANUAL (n) 146512 B

DIREKTORATET FOR PATENT- OG VAREMÆRKEVÆSENETDIRECTORATE OF THE PATENT AND TRADEMARKET SYSTEM

(21) Patentansøgning nr.: 3850/77 (51) Int.CL3: C 12 N 1/14 (22) Indleveringsdag: 30 aug 1977 (41) Aim. tilgængelig: 01 mar 1978 (44) Fremlagt: 24 okt 1983 (86) International ansøgning nr.: -(30) Prioritet: 30aug1976JP51/102628 (71) Ansøger: ‘KUREHA KAGAKU KOGYO KABUSHIKIKAISHA; Tokyo, JP.(21) Patent Application No: 3850/77 (51) Int.CL3: C 12 N 1/14 (22) Filing Date: Aug 30, 1977 (41) Aim. available: 01 Mar 1978 (44) Submitted: 24 Oct 1983 (86) International Application No: - (30) Priority: 30Aug1976JP51 / 102628 (71) Applicant: 'KUREHA KAGAKU KOGYO KABUSHIKIKAISHA; Tokyo, JP.

(72) Opfinder: Chlkao ‘Yoshikumi; JP, Takao ‘Furusho; JP, Kenlchi ‘Matsunaga; JP, Norlyuki *Toyoda; JP.(72) Inventor: Chlkao 'Yoshikumi; JP, Takao 'Furusho; JP, Kenlchi 'Matsunaga; JP, Norlyuki * Toyoda; JP.

(74) Fuldmægtig: Ingeniørfirmaet Lehmann & Ree (54) Fremgangsmåde til dyrkning af Basidiomycet-svampen Coriolus versicolor (Fr.) Quel.(74) Plenipotentiary: Lehmann & Ree Engineering Company (54) Process for cultivating the Basidiomycet fungus Coriolus versicolor (Fr.) Quel.

Opfindelsen angår en fremgangsmåde til dyrkning af Basi-diomycetsvampen Coriolus versicolor (Fr.) Quel. i et dyrkningsmedium.The invention relates to a method for growing the Basiomyomycetus fungus Coriolus versicolor (Fr.) Quel. in a culture medium.

De af Basidiomyceterne hørende til slægten Coriolus afThose of the Basidiomycetes belonging to the genus Coriolus of

Polyporaceae-familien frembragte polysaccharider har i de senere ^ år tiltrukket sig opmærksomheden som følge af deres høje antitumor- igene virkning og andre farmakologiske virkninger, og der er opstået ΓΜ ^ behov for at kunne gennemføre industriel produktion af disse stoffer X) på fordelagtig måde.The Polyporaceae family produced polysaccharides have attracted attention in recent years due to their high antitumor and other pharmacological effects, and there has been a need to favor industrial production of these substances (X).

J Ved dyrkning af Basidiomyceterne har man tidligere almin- ^ deligvis benyttet et substrat af den type, der sædvanligvis benyttes 2) til dyrkning af mikroorganismer, f.eks. et pepton-substrat indehol dende glucose og gærekstrakt.In the cultivation of the Basidiomycetes, a substrate of the type usually used 2) for the cultivation of microorganisms, e.g. a peptone substrate containing glucose and yeast extract.

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Fra beskrivelserne til dansk pat.ans.nr. 3340/77 og 3476/77 er det endvidere kendt at dyrke organismen Coriolus versicolor i et glucose-gærekstraktsubstrat indeholdende 10 vægtprocent glucose, 1,5 vægtprocent gærekstrakt, 0,1 vægtprocent og 0,1 vægtpro cent MgSO^^I^O henholdsvis 15 vægtprocent glucose, 2,25 vægtprocent gærekstrakt, 0,1 vægtprocent KH^PO^, 0,1 vægtprocent MgSO^^I^O og 0,2 vægtprocent maltekstrakt.From the descriptions to Danish Pat. 3340/77 and 3476/77, it is further known to grow the organism Coriolus versicolor in a glucose yeast extract substrate containing 10 wt.% Glucose, 1.5 wt.% Yeast extract, 0.1 wt.% And 0.1 wt.% MgSO wt% glucose, 2.25 wt% yeast extract, 0.1 wt% KH ^ PO ^, 0.1 wt% MgSO ^^ I ^ O, and 0.2 wt% malt extract.

Det har imidlertid nu vist sig, at det ved dyrkning af organismen Coriolus versicolor er muligt at forøge udbyttet af mycelium væsentligt uden tilsætning af uorganiske salte til dyrkningsmediet, således som det hidtil har været anset for nødvendigt ved dyrkning af nævnte Basidiomycetsvamp. Ved industriel dyrkning af svampen betyder dette en væsentlig simplificering af dyrkningsmediets sammensætning, som igen indebærer en væsentlig økonomisk fordel, ikke blot på grund af mindre udgifter til kemikalier, men også på grund af mindre krav til apparatur og driftsbetingelser.However, it has now been found that by growing the organism Coriolus versicolor it is possible to significantly increase the yield of mycelium without the addition of inorganic salts to the culture medium, as has hitherto been deemed necessary in the cultivation of said Basidiomycetus fungus. In industrial cultivation of the fungus, this means a significant simplification of the composition of the culture medium, which in turn implies a significant economic advantage, not only because of lower cost of chemicals, but also because of lower requirements for equipment and operating conditions.

Dette opnås ved en fremgangsmåde af den i indledningen omtalte art, som ifølge opfindelsen er ejendommelig ved, at dyrkningsmediet udelukkende består af 7,5-15 vægtprocent glucose, gærekstrakt og vand, hvorhos vægtforholdet mellem glucose og gærekstrakt er 3-15:1.This is achieved by a method of the kind mentioned in the introduction, which according to the invention is characterized in that the culture medium consists solely of 7.5-15% by weight glucose, yeast extract and water, the weight ratio of glucose to yeast extract being 3-15: 1.

Det skal bemærkes, at selv hvis forholdet mellem glucose og gærekstrakt i substratsammensætningen ligger i området fra 3-15:1, fås ingen væsentlig forøgelse af mycelieudbyttet, når glucoseindhol-det i mediet er lavere end 7,5%. Udbyttet ændres også kun lidt, hvis glucoseindholdet overstiger 15%, således at brug af glucose udover 15% ikke er hensigtsmæssigt i økonomisk henseende. Når nævnte Basi-diomycet dyrkes under anvendelse af et glucose-gærekstraktsubstrat med ovennævnte sammensætning ifølge opfindelsen, opnås der et mycelie-udbytté, der er 2-5 gange så højt som når Basidiomyceten dyrkes under anvendelse af sædvanlige substrater, f.eks. naturlige substrater, såsom pepton-substrat eller modificeret pepton-substrat, maltekstraktsubstrat, majsekstraktsubstrat eller majsstøbevandssubstrat eller syntetiske subtrater, såsom Pfeffer's substrat, Sabouraud's substrat eller Meyer's substrat. Fremgangsmåden ifølge opfindelsen tilvejebringer også 2-4 gange så højt mycelieudbytte som når der anvendes et glucose-gærekstraktsubstrat, der har en sammensætning udenfor det ifølge den foreliggende opfindelse angivne område. Med hensyn til sammensætningen af det ifølge den foreliggende opfindelse anvendte dyrkningsmedium foretrækkes det for at tilvejebringe det 3 1A 6 512 højeste udbytte at benytte et dyrkningsmedium, hvori glucoseindhol-det udgør fra 10 til 15 vægtprocent af dyrkningsmediet, og/eller at glucose/gærekstraktforholdet ligger i området 6-10:1.It should be noted that even if the ratio of glucose to yeast extract in the substrate composition is in the range of 3-15: 1, no significant increase in mycelial yield is obtained when the glucose content of the medium is lower than 7.5%. The yield also changes only slightly if the glucose content exceeds 15%, so that the use of glucose beyond 15% is not economically appropriate. When said Basidiomycet is grown using a glucose yeast extract substrate of the above composition of the invention, a mycelial yield is obtained which is 2-5 times as high as when the Basidiomycet is grown using conventional substrates, e.g. natural substrates such as peptone substrate or modified peptone substrate, malt extract substrate, corn extract substrate or maize cast water substrate or synthetic substrates such as Pfeffer's substrate, Sabouraud's substrate or Meyer's substrate. The process of the invention also provides 2-4 times as high mycelial yield as when using a glucose yeast extract substrate having a composition outside the range of the present invention. With regard to the composition of the culture medium used according to the present invention, it is preferable to use the culture medium in which the glucose content is from 10 to 15% by weight of the culture medium, and / or that the glucose / yeast extract ratio is in the preferred medium. in the range of 6-10: 1.

For så vidt angår de ifølge opfindelsen anvendte dyrkningsbetingelser gennemføres dyrkningen generelt ved en temperatur på 25 +3°C over en periode på 3-10 dage i tilfælde af submers dyrkning under beluftning og omrøring og 20-30 dage i tilfælde af stationær dyrkning.As to the cultivation conditions used in accordance with the invention, cultivation is generally carried out at a temperature of 25 + 3 ° C over a period of 3-10 days in the case of submergence under aeration and stirring and 20-30 days in the case of stationary cultivation.

Basidiomyceten hørende til slægten Coriolus af Polypora-ceae-familien, som benyttes i forbindelse med udførelse af den foreliggende opfindelse, er velkendt indenfor fagområdet, og dens mykologiske egenskaber er beskrevet i "Colored Illustration of Fungi of Japan" af Rokuya Imazeki og Tsuguo Hongo, bind I, 1975, og II, 1974. Arten er deponeret i The Fermentation Research Institute, Agency of Industrial Science and Technology (Chiba-shi, Japan) under den japanske regering:The basidiomycetes of the genus Coriolus of the Polypora ceae family used in the practice of the present invention are well known in the art, and its mycological properties are described in "Colored Illustration of Fungi of Japan" by Rokuya Imazeki and Tsuguo Hongo, volumes I, 1975, and II, 1974. The species is deposited in The Fermentation Research Institute, Agency of Industrial Science and Technology (Chiba-shi, Japan) under the Japanese Government:

Art Deponeringsnr. DeponeringsdatoArt Deposit no. Date of Deposit

Coriolus versicolor (Fr.) Quel. CM-103 FERM-P 2414 25. dec. 1973Coriolus versicolor (Fr.) Quel. CM-103 FERM-P 2414 Dec 25 1973

Det ved dyrkning af ovennævnte Basidiomycet hørende til slægten Coriolus fremkomne mycelium ekstraheres med et vandigt opløsningsmiddel, såsom vand eller en fortyndet alkalisk opløsning, og den fremkomne ekstraktopløsning renses for at fjerne den lavmolekylære fraktion, og remanensen tørres yderligere, hvorved der fremkommer et stof bestående af polysaccharid. Det således fremkomne polysaccharid indeholder nitrogen i sine molekyler og har særdeles god antitumorigen effekt. Når mus indgives disse stoffer, udviser stofferne særdeles god antitumorigen aktivitet, ikke blot ved intraperitoneal indgivelse men også ved oral indgivelse. Der er allerede fremkommet mange rapporter over dette stofs antitumorigene effekt. Brugen af stoffet er ikke begrænset til sådanne antitumorigene anvendelser, men resultaterne af prøverne har vist dets særdeles gode evne til fremkaldelse af immunitet overalt i værtsorganismen (eng.: recovery of immunity through the host), forhindring af bivirkninger ved kemoterapi og forhindring af, at en patients fysiske tilstand forringes efter en operation eller af andre årsager. Det bemærkedes også ved forsøgene, at oral indgivelse af dette stof har den virkning, at appetitten forøges, tarmforstyrrelser helbredes og urinering fremmes.The mycelium obtained by culturing the above Basidiomycet of the genus Coriolus is extracted with an aqueous solvent such as water or a dilute alkaline solution and the resulting extract solution is purified to remove the low molecular fraction and the residue is further dried to give a substance consisting of polysaccharide. The polysaccharide thus obtained contains nitrogen in its molecules and has very good antitumorigenic effect. When mice are administered these substances, the substances exhibit very good antitumorigenic activity, not only by intraperitoneal administration but also by oral administration. Many reports have already been published about the antitumorigenic effect of this drug. The use of the drug is not limited to such antitumor-rich applications, but the results of the tests have shown its extremely good ability to induce immunity throughout the host organism, the prevention of chemotherapy side effects and the prevention of a patient's physical condition deteriorates after surgery or for other reasons. It was also noted in the experiments that oral administration of this drug has the effect of increasing appetite, healing of intestinal disorders and promoting urination.

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Opfindelsen vil nu blive beskrevet mere detaljeret ved hjælp af en udførelsesform. Procentangivelser i nedenstående eksempel er på vægtbasis.The invention will now be described in more detail by way of an embodiment. The percentages in the example below are by weight.

Eksempel 20 ml af hvert af substraterne med den i tabel 1 viste sammensætning anbragtes i en 100 ml konisk kolbe,og hvert substrat indpodedes en podekultur af Coriolus versicolor (Fr.) Quel. CM-103 (FERM-P nr. 2414) og underkastedes stationær dyrkning ved 25-28°C i 28 dage. Efter afslutning af dyrkningen bestemtes det mycelieudbytte, der var dannet i hver kolbe, hvorved de i tabel 1 viste resultater opnåedes.Example 20 ml of each of the substrates of the composition shown in Table 1 was placed in a 100 ml conical flask and each substrate was inoculated with a seed culture of Coriolus versicolor (Fr.) Quel. CM-103 (FERM-P No. 2414) and subjected to stationary culture at 25-28 ° C for 28 days. At the end of the culture, the mycelial yield formed in each flask was determined and the results shown in Table 1 were obtained.

I tabel 1 er også vist resultaterne af sammenligningseksempler, hvor dyrkning gennemførtes under samme betingelser som beskrevet ovenfor med undtagelse af, at glucose-gærekstraktsubstrater med sammensætninger udenfor det ifølge opfindelsen specificerede område benyttedes.Table 1 also shows the results of comparative examples where cultivation was carried out under the same conditions as described above except that glucose yeast extract substrates having compositions outside the range specified by the invention were used.

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Tabel 1Table 1

Glucose Gærekstrakt Glucose/gæreks- Mycelieudbytte (S) (%) trakt (g/1) 15 1,0 15 28,7 15 1,25 12 33,0 15 1,5 10 39,1 h ΓΤ) 12.5 1,0 12,5 34,2 hGlucose Yeast extract Glucose / yeast extract Mycelial yield (S) (%) funnel (g / 1) 15 1.0 15 28.7 15 1.25 12 33.0 15 1.5 10 39.1 h ΓΤ) 12.5 1.0 12.5 34.2 h

12.5 1,25 10 39,2 Q12.5 1.25 10 39.2 Q

12.5 1,5 8,3 42,9 £ 10.0 0,75 13,3 30,7 £ 10.0 1,0 10 35,4 ro 10.0 1,25 8,0 41,2 “ 10.0 1,5 6,7 40,6 3 7.5 0,5 15 27,6 7.5 0,75 10 33,7 7.5 1,0 7,5 32,2 7.5 1,25 6 30,312.5 1.5 8.3 42.9 £ 10.0 0.75 13.3 30.7 £ 10.0 1.0 10 35.4 ro 10.0 1.25 8.0 41.2 “10.0 1.5 6.7 40 , 6 3 7.5 0.5 15 27.6 7.5 0.75 10 33.7 7.5 1.0 7.5 32.2 7.5 1.25 6 30.3

(D CO(D CO

2.5 0,25 10 11,0 gf| 5,0 0,5 10 23,3 | ro 7.5 0,25 30 18,8 £ £ 10.0 0,5 20 23,1 * s 12.5 0,5 25 15,9 jg 15.0 0,75 20 22,8 “ 2.5 1,25 2 5,82.5 0.25 10 11.0 gf | 5.0 0.5 10 23.3 | ro 7.5 0.25 30 18.8 £ 10.0 0.5 20 23.1 * s 12.5 0.5 25 15.9 jg 15.0 0.75 20 22.8 “2.5 1.25 2 5.8

Det fremgår af resultaterne i ovenstående tabel, at det mycelieudbytte, der opnås ved dyrkning ifølge fremgangsmåden ifølge opfindelsen, er betydeligt forbedret i forhold til sammenlignings-eksemplerne.It can be seen from the results in the above table that the mycelial yield obtained by cultivation according to the method of the invention is significantly improved over the comparative examples.

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Sammlignende afprøvning.Comparative testing.

Til belysning af anvendeligheden af det ved fremgangsmåden ifølge nærværende opfindelse benyttede dyrkningssubstrat i sammenligning med de dyrkningssubstrater, der benyttes ved de fra beskrivelsen til dansk pat.ans.nr. 3340/77 og nr. 3476/77 kendte fremgangsmåder, er nedenstående forsøg blevet udført.In order to illustrate the utility of the culture substrate used in the process of the present invention in comparison with the culture substrates used in those disclosed in the specification of Danish Pat. 3340/77 and No. 3476/77 known procedures, the following experiments have been carried out.

20 ml af hver af de i efterfølgende tabel 2 og 3 anførte dyrkningsmedier, som havde de i tabellerne anførte sammensætninger, blev hver for sig anbragt i en 100 ml konisk flaske, og hvert medium blev podet med en podekultur af Coriolus versicolor (Fr.) Quél.20 ml of each of the culture media listed in subsequent Tables 2 and 3, which had the compositions listed in the tables, were separately placed in a 100 ml conical flask and each medium was seeded with a seed culture of Coriolus versicolor (Fr.). Quel.

CM-103 (FERM-P nr. 2414) og dyrket under stationære betingelser ved 25-28°C i 28 dage. Efter afslutning af dyrkningen blev udbyttet af det i hver flaske frembragte mycelium bestemt, og de opnåede resultater er anført i tabel 2 og 3.CM-103 (FERM-P No. 2414) and grown under stationary conditions at 25-28 ° C for 28 days. After completion of the culture, the yield of the mycelium produced in each bottle was determined and the results obtained are given in Tables 2 and 3.

TABEL 2TABLE 2

Forsøg Forskrift Glucose Gæreks- K^PO^ MgSO^ELjO Udbytte “· (%) trakt 1,1 (%) (%> (g/liter) 1 Iflg.opfindelsen 10 1,5 0 0 40,6 2 Iflg.DK pat.ans.Test Regulations Glucose Yeast Exercise K ^ PO ^ MgSO ^ ELjO Yield “· (%) funnel 1.1 (%) (%> (g / liter) 1 According to Invention 10 1.5 0 0 40.6 2 pat.ans.

nr. 3340/77 10 1,5 0,1 0,1 38,0 TABEL 3No. 3340/77 10 1.5 0.1 0.1 38.0 TABLE 3

Forsøg Forskrift Glucose Gæreks- KH-PO. MgS0,.7H90 Malteks- nr. (%) trakt (%) (%j ^ trakt (%) 3 Iflg.opfindelsen 10 1,5 0 0 0 4 Iflg.DK pat.ans.Experiment Regulations Glucose Yeast Ex- KH-PO. MgS0, .7H90 Malt text no. (%) Funnel (%) (% J funnel (%) 3 According to the invention 10 1.5 0 0 0 4 According to Pat.

nr. 3476/77 10 1,5 0,1 0,1 0,2 5 do 3476/77 10 1,5 0 0 0,2No. 3476/77 10 1.5 0.1 0.1 0.2 5 do 3476/77 10 1.5 0 0 0.2

Forsøg Udbytte nr. (%) 3 40,6 4 38,9 5 39,1Experiment Yield No. (%) 3 40.6 4 38.9 5 39.1

DK385077A 1976-08-30 1977-08-30 PROCEDURE FOR CULTIVATING THE BASIDIOMYCETE FUNGI CORIOLUS VERSICOLOR (FR.) QUEL. DK146512C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP10262876 1976-08-30
JP10262876A JPS5329987A (en) 1976-08-30 1976-08-30 Cultivation of basidiomycetes belonging to coriolus genus

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DK385077A DK385077A (en) 1978-03-01
DK146512B true DK146512B (en) 1983-10-24
DK146512C DK146512C (en) 1984-04-02

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US (1) US4162939A (en)
JP (1) JPS5329987A (en)
AR (1) AR214337A1 (en)
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US4369253A (en) * 1979-06-11 1983-01-18 Kureha Kagaku Kogyo Kabushiki Kaisha Growth promoting method for basidiomycetes
US4327181A (en) * 1980-05-15 1982-04-27 Battelle Development Corporation Aerobic submerged fermentation of sporulating, ectomycorrhizal fungi
US4689667A (en) * 1985-06-11 1987-08-25 Fairchild Semiconductor Corporation Method of controlling dopant diffusion and dopant electrical activation by implanted inert gas atoms
US4830708A (en) * 1987-11-30 1989-05-16 Pulp And Paper Research Institute Of Canada Direct biological bleaching of hardwood kraft pulp with the fungus Coriolus versicolor
JPH0643336B2 (en) * 1988-06-30 1994-06-08 呉羽化学工業株式会社 Vascular growth inhibitor
ES2040664B1 (en) * 1992-01-13 1994-05-16 Llora Ramon Espigol PROCEDURE FOR OBTAINING A MICORRIZED TREE FOR REFORESTATION.
JP3651697B2 (en) * 1995-03-24 2005-05-25 株式会社アミノアップ化学 New polysaccharide substances
US20040106187A1 (en) * 2002-11-30 2004-06-03 Nam-Doo Hong Composition for the culturing of Phellinus linteus mycelium
JP5818419B2 (en) * 2009-10-27 2015-11-18 タカラバイオ株式会社 Manufacturing method of Bunashimeji fruiting body
RU2601589C2 (en) * 2014-10-10 2016-11-10 Федеральное государственное бюджетное научное учреждение "Дальневосточный научно-исследовательский институт сельского хозяйства" (ФГБНУ "ДВ НИИСХ") Method for intensive cultivation of hericium erinaceus

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US3301848A (en) * 1962-10-30 1967-01-31 Pillsbury Co Polysaccharides and methods for production thereof
US3759896A (en) * 1968-03-28 1973-09-18 T Yamamoto Process for manufacture of polysaccharides with antitumor action
US3754925A (en) * 1970-03-24 1973-08-28 Takeda Chemical Industries Ltd New thermo gelable polysaccharide containing foodstuffs
JPS5339519B1 (en) * 1970-07-31 1978-10-21
US4051314A (en) * 1970-10-14 1977-09-27 Kureha Kagaku Kogyo Kabushiki Kaisha Polysaccharides and method for producing same
US3960832A (en) * 1973-10-05 1976-06-01 Kenneth Suk Kang Polysaccharide and bacterial fermentation process for its preparation

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SU718018A3 (en) 1980-02-25
CH632639A5 (en) 1982-10-29
DK146512C (en) 1984-04-02
ZA774933B (en) 1978-06-28
FR2362579B1 (en) 1980-06-06
JPS5532355B2 (en) 1980-08-25
AT359185B (en) 1980-10-27
AR214337A1 (en) 1979-05-31
CA1081636A (en) 1980-07-15
FR2362579A1 (en) 1978-03-24
GB1551476A (en) 1979-08-30
DD132880A5 (en) 1978-11-15
US4162939A (en) 1979-07-31
BR7705745A (en) 1978-06-06
AU2835977A (en) 1979-03-08
JPS5329987A (en) 1978-03-20
ATA620877A (en) 1980-03-15
HU175949B (en) 1980-11-28
YU197177A (en) 1982-08-31
DE2737471C2 (en) 1982-09-16
ES461819A1 (en) 1978-08-16
CS200223B2 (en) 1980-08-29
DK385077A (en) 1978-03-01
PH12089A (en) 1978-10-30
BG37998A3 (en) 1985-09-16
YU39814B (en) 1985-04-30
AU504111B2 (en) 1979-10-04
PL200532A1 (en) 1978-06-19
MX4417E (en) 1982-04-28
NL7709200A (en) 1978-03-02
PL104871B1 (en) 1979-09-29
NL176374B (en) 1984-11-01
DE2737471A1 (en) 1978-03-02
SE432947B (en) 1984-04-30
BE858191A (en) 1978-02-28
IT1085028B (en) 1985-05-28
SE7709667L (en) 1978-03-01
RO71955A (en) 1982-02-26

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