DK1320626T3 - Fermentation Process for Preparation of L-Amino Acids Using Strings from the Enterobacteriaceae Family - Google Patents

Fermentation Process for Preparation of L-Amino Acids Using Strings from the Enterobacteriaceae Family

Info

Publication number
DK1320626T3
DK1320626T3 DK01974228.7T DK01974228T DK1320626T3 DK 1320626 T3 DK1320626 T3 DK 1320626T3 DK 01974228 T DK01974228 T DK 01974228T DK 1320626 T3 DK1320626 T3 DK 1320626T3
Authority
DK
Denmark
Prior art keywords
preparation
amino acids
fermentation process
strings
amino acid
Prior art date
Application number
DK01974228.7T
Other languages
Danish (da)
Inventor
Mechthild Rieping
Christine Bastuck
Thomas Hermann
Georg Thierbach
Original Assignee
Evonik Degussa Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evonik Degussa Gmbh filed Critical Evonik Degussa Gmbh
Priority claimed from PCT/EP2001/010209 external-priority patent/WO2002029080A2/en
Application granted granted Critical
Publication of DK1320626T3 publication Critical patent/DK1320626T3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli

Landscapes

  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention relates to a fermentation process for the preparation of L-amino acids, especially L-threonine, in which the following steps are carried out: a) fermentation of the microorganisms of the family Enterobacteriaceae producing the desired L-amino acid, in which microorganisms at least the pckA gene and/or the open reading frames yjfA and ytfP are, individually or jointly, attenuated and, in particular, switched off, b) enrichment of the L-amino acid in the medium or in the bacterial cells, and c) isolation of the L-amino acid.
DK01974228.7T 2000-09-30 2001-09-05 Fermentation Process for Preparation of L-Amino Acids Using Strings from the Enterobacteriaceae Family DK1320626T3 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE10048605 2000-09-30
DE10055516 2000-11-09
DE10130192A DE10130192A1 (en) 2000-09-30 2001-06-22 Process for the fermentative production of L-amino acids using strains of the Enterobacteriaceae family
PCT/EP2001/010209 WO2002029080A2 (en) 2000-09-30 2001-09-05 Fermentation process for the preparation of l-amino acids using strains of the family enterobacteriaceae

Publications (1)

Publication Number Publication Date
DK1320626T3 true DK1320626T3 (en) 2010-03-22

Family

ID=26007228

Family Applications (1)

Application Number Title Priority Date Filing Date
DK01974228.7T DK1320626T3 (en) 2000-09-30 2001-09-05 Fermentation Process for Preparation of L-Amino Acids Using Strings from the Enterobacteriaceae Family

Country Status (6)

Country Link
AT (1) ATE447618T1 (en)
DE (2) DE10130192A1 (en)
DK (1) DK1320626T3 (en)
ES (1) ES2336192T3 (en)
PL (1) PL210791B1 (en)
PT (1) PT1320626E (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10147960A1 (en) * 2001-09-28 2003-04-10 Degussa Process for the fermentative production of D-pantothenic acid and / or its salts
WO2018007565A1 (en) * 2016-07-08 2018-01-11 Metabolic Explorer Method for the fermentative production of methionine or its hydroxy analog form by microorganisms comprising genes coding sugar phosphotransferase system (pts)

Also Published As

Publication number Publication date
ATE447618T1 (en) 2009-11-15
PT1320626E (en) 2010-02-02
DE60140373D1 (en) 2009-12-17
PL210791B1 (en) 2012-03-30
ES2336192T3 (en) 2010-04-09
DE10130192A1 (en) 2002-04-11

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