DK128687D0 - PROTEINS AND THEIR PREPARATION AND USE - Google Patents

PROTEINS AND THEIR PREPARATION AND USE

Info

Publication number
DK128687D0
DK128687D0 DK128687A DK128687A DK128687D0 DK 128687 D0 DK128687 D0 DK 128687D0 DK 128687 A DK128687 A DK 128687A DK 128687 A DK128687 A DK 128687A DK 128687 D0 DK128687 D0 DK 128687D0
Authority
DK
Denmark
Prior art keywords
insecticidal
proteinaceous substance
coding
dna
dna fragment
Prior art date
Application number
DK128687A
Other languages
Danish (da)
Other versions
DK128687A (en
Inventor
Martin Geiser
Albert Hinnen
Jakob Brassel
Silvia Schweitzer-Gruetzmacher
Original Assignee
Ciba Geigy Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ciba Geigy Ag filed Critical Ciba Geigy Ag
Publication of DK128687D0 publication Critical patent/DK128687D0/en
Publication of DK128687A publication Critical patent/DK128687A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal protein (delta-endotoxin)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor

Abstract

The present invention is directed to a method for producing an insecticidal proteinaceous substance including the discovery and identification of the entire DNA sequence coding for the insecticidal protein MGE 1 of said proteinaceous substance, to said proteinaceous substance, to a DNA fragment characterized by the nucleotide sequence given in table 2, said fragment coding for the protein MGE 1, to the protein MGE 1 itself, to a DNA fragment originating from Bacillus thuringiensis var.kurstaki from Hpal (O) to Pstl (4355) coding for an insecticidal proteinaceous substance including truncated portions thereof, said truncated DNA portions being subject to the proviso that insecticidal activity has not been lost, to the method of constructing cloning vehicles and expression vehicles comprising the DNA fragment given in table 2 and also to said vehicles themselves.
DK128687A 1986-03-15 1987-03-13 PROTEINS AND THEIR PREPARATION AND USE DK128687A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB8606441A GB2188049B (en) 1986-03-15 1986-03-15 Insecticidal proteinaceous substance

Publications (2)

Publication Number Publication Date
DK128687D0 true DK128687D0 (en) 1987-03-13
DK128687A DK128687A (en) 1987-09-16

Family

ID=10594681

Family Applications (1)

Application Number Title Priority Date Filing Date
DK128687A DK128687A (en) 1986-03-15 1987-03-13 PROTEINS AND THEIR PREPARATION AND USE

Country Status (25)

Country Link
EP (1) EP0238441B1 (en)
JP (1) JPS62224295A (en)
KR (1) KR900007640B1 (en)
AT (1) ATE97163T1 (en)
AU (1) AU608508B2 (en)
BG (2) BG46752A3 (en)
BR (1) BR8701162A (en)
DD (2) DD297763A5 (en)
DE (1) DE3788077D1 (en)
DK (1) DK128687A (en)
EG (1) EG18869A (en)
ES (1) ES2059404T3 (en)
FI (1) FI871070A (en)
GB (1) GB2188049B (en)
HU (1) HU204092B (en)
IE (1) IE59456B1 (en)
IL (1) IL81886A (en)
MA (1) MA20900A1 (en)
NO (1) NO871062L (en)
NZ (1) NZ219611A (en)
PL (1) PL154252B1 (en)
PT (1) PT84474B (en)
TN (1) TNSN87038A1 (en)
TR (1) TR23521A (en)
ZA (1) ZA871830B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0317511A3 (en) * 1987-11-18 1991-10-16 Ciba-Geigy Ag Insecticidal cotton plant cells
CA2101610A1 (en) * 1992-08-07 1994-02-08 William D. Prevatt Production of bacillus entomotoxins in methylotrophic yeast
GB9618083D0 (en) * 1996-08-29 1996-10-09 Mini Agriculture & Fisheries Pesticidal agents

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57142907A (en) * 1981-02-27 1982-09-03 Shionogi & Co Ltd Bacterium-originated insecticide and its preparation
WO1982003872A1 (en) * 1981-04-27 1982-11-11 Washington Univ B.thuringiensis crystal protein in e.coli
FR2525630B1 (en) * 1982-04-26 1985-07-05 Pasteur Institut DNA CONTAINING A SEQUENCE ENCODING A CRYSTAL PROTEIN OR A POLYPEPTIDE HAVING INSECTICIDE PROPERTIES, MICROORGANISMS TRANSFORMED BY SUCH DNA, COMPOSITIONS CONTAINING SAID CRYSTAL PROTEINS, POLYPEPTIDE OR MICROORGANISMS
GB2125047B (en) * 1982-08-09 1986-02-19 Ciba Geigy Ag Yeast hybrid vectors and their use for the production of polypeptides
CA1301094C (en) * 1984-08-31 1992-05-19 Helen Riaboff Whiteley Bacillus thuringiensis crystal protein gene toxin segment
GB8425487D0 (en) * 1984-10-09 1984-11-14 Agricultural Genetics Co Strain of bacillus thuringiensis
ZA859400B (en) * 1984-12-10 1986-10-29 Monsanto Co Insertion of the bacillus thuringiensis crystal protein gene into plant-colonizing microorganisms and their use
CA1341283C (en) * 1985-03-28 2001-08-14 Frank H. Gaertner Biological pesticides and methods for their delivery and use
JPS62100292A (en) * 1985-10-28 1987-05-09 Sumitomo Chem Co Ltd Insecticidal protein gene of bacillus thuringensis aizawai ipl strain
CA1341092C (en) 1985-12-12 2000-09-05 David L. Edwards Process for altering the host range of bacillus thuringiensis toxins, and novel toxins produced thereby

Also Published As

Publication number Publication date
NZ219611A (en) 1991-01-29
NO871062D0 (en) 1987-03-13
HU204092B (en) 1991-11-28
IL81886A (en) 1992-02-16
PT84474A (en) 1987-04-01
DE3788077D1 (en) 1993-12-16
IE59456B1 (en) 1994-02-23
GB2188049A (en) 1987-09-23
KR900007640B1 (en) 1990-10-17
ES2059404T3 (en) 1994-11-16
DK128687A (en) 1987-09-16
EG18869A (en) 1994-02-28
PT84474B (en) 1989-11-10
ZA871830B (en) 1987-11-25
EP0238441A3 (en) 1988-08-10
BG46006A3 (en) 1989-09-15
DD259420A5 (en) 1988-08-24
MA20900A1 (en) 1987-10-01
AU608508B2 (en) 1991-04-11
DD297763A5 (en) 1992-01-23
TNSN87038A1 (en) 1990-01-01
EP0238441A2 (en) 1987-09-23
BG46752A3 (en) 1990-02-15
HUT43628A (en) 1987-11-30
PL264628A1 (en) 1988-04-28
ATE97163T1 (en) 1993-11-15
JPS62224295A (en) 1987-10-02
IE870665L (en) 1987-09-15
TR23521A (en) 1990-03-01
PL154252B1 (en) 1991-07-31
GB2188049B (en) 1990-09-05
NO871062L (en) 1987-09-16
KR870009016A (en) 1987-10-22
FI871070A0 (en) 1987-03-11
BR8701162A (en) 1988-01-12
FI871070A (en) 1987-09-16
AU6999287A (en) 1987-09-17
IL81886A0 (en) 1987-10-20
EP0238441B1 (en) 1993-11-10

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Legal Events

Date Code Title Description
AHB Application shelved due to non-payment