DK0743948T4 - Chromatografisk isolering af nucleinsyrer - Google Patents
Chromatografisk isolering af nucleinsyrerInfo
- Publication number
- DK0743948T4 DK0743948T4 DK95907640T DK95907640T DK0743948T4 DK 0743948 T4 DK0743948 T4 DK 0743948T4 DK 95907640 T DK95907640 T DK 95907640T DK 95907640 T DK95907640 T DK 95907640T DK 0743948 T4 DK0743948 T4 DK 0743948T4
- Authority
- DK
- Denmark
- Prior art keywords
- nucleic acids
- pct
- chromatographic isolation
- sec
- date
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Saccharide Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE4403692 | 1994-02-07 | ||
DE4403693 | 1994-02-07 | ||
DE4431125 | 1994-09-01 | ||
DE4432654A DE4432654C2 (de) | 1994-09-14 | 1994-09-14 | Verfahren zur Isolierung von Nukleinsäuren aus natürlichen Quellen |
PCT/EP1995/000390 WO1995021178A1 (de) | 1994-02-07 | 1995-02-03 | Steigerung der transfektionseffizienz von nucleinsäuren durch die verwendung von isopropanol in wässrigen lösungen |
Publications (2)
Publication Number | Publication Date |
---|---|
DK0743948T3 DK0743948T3 (da) | 2000-01-31 |
DK0743948T4 true DK0743948T4 (da) | 2004-02-02 |
Family
ID=27435910
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK95907640T DK0743948T4 (da) | 1994-02-07 | 1995-02-03 | Chromatografisk isolering af nucleinsyrer |
Country Status (9)
Country | Link |
---|---|
US (1) | US5792651A (da) |
EP (1) | EP0743948B2 (da) |
JP (1) | JP3379758B2 (da) |
AT (1) | ATE181921T1 (da) |
AU (1) | AU684134B2 (da) |
CA (1) | CA2182398C (da) |
DE (1) | DE59506355D1 (da) |
DK (1) | DK0743948T4 (da) |
WO (1) | WO1995021178A1 (da) |
Families Citing this family (25)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7026468B2 (en) * | 1996-07-19 | 2006-04-11 | Valentis, Inc. | Process and equipment for plasmid purification |
US7807822B2 (en) * | 1996-08-01 | 2010-10-05 | Robert Bridenbaugh | Methods for purifying nucleic acids |
US7569342B2 (en) | 1997-12-10 | 2009-08-04 | Sierra Molecular Corp. | Removal of molecular assay interferences |
US7078224B1 (en) | 1999-05-14 | 2006-07-18 | Promega Corporation | Cell concentration and lysate clearance using paramagnetic particles |
DE19836559A1 (de) | 1998-08-12 | 2000-03-23 | Antigen Gmbh | Gefäß zur Entnahme von Blut |
EP0987327A1 (en) * | 1998-09-14 | 2000-03-22 | QIAGEN GmbH | Novel method for purifying covalently closed circular DNA |
EP0992583B1 (en) * | 1998-09-14 | 2003-03-26 | QIAGEN GmbH | Novel method for purifying covalently closed circular DNA |
DE19903507A1 (de) * | 1999-01-29 | 2000-08-10 | Roche Diagnostics Gmbh | Verfahren zur Herstellung endotoxinfreier oder an Endotoxin abgereicherter Nukleinsäuren und deren Verwendung |
US6270970B1 (en) | 1999-05-14 | 2001-08-07 | Promega Corporation | Mixed-bed solid phase and its use in the isolation of nucleic acids |
US6602718B1 (en) | 2000-11-08 | 2003-08-05 | Becton, Dickinson And Company | Method and device for collecting and stabilizing a biological sample |
GB0216648D0 (en) * | 2002-07-18 | 2002-08-28 | Lonza Biologics Plc | Method of expressing recombinant protein in CHO cells |
AU2003267175A1 (en) * | 2002-09-13 | 2004-04-30 | Valentis, Inc. | Apparatus and method for preparative scale purification of nucleic acids |
JP2009500019A (ja) * | 2005-07-01 | 2009-01-08 | プロメガ・コーポレーション | 生体分子の精製のための浮遊性粒子のネットワーク、及び生体分子の精製のための浮遊性粒子又は浮遊性粒子のネットワークの使用 |
EP1963526A4 (en) * | 2005-12-09 | 2009-11-18 | Promega Corp | PURIFICATION OF NUCLEIC ACID WITH A BINDING MATRIX |
TW200930811A (en) * | 2008-01-04 | 2009-07-16 | Univ Nat Taiwan Science Tech | Transfection reagent and method for enhancing transfection efficiency |
US20110020924A1 (en) * | 2008-04-30 | 2011-01-27 | Gradalis, Inc. | Highly pure plasmid dna preparations |
US20120231537A1 (en) * | 2008-04-30 | 2012-09-13 | Gradalis, Inc. | Highly Pure Plasmid DNA Preparations |
US8222397B2 (en) * | 2009-08-28 | 2012-07-17 | Promega Corporation | Methods of optimal purification of nucleic acids and kit for use in performing such methods |
US8039613B2 (en) | 2009-08-28 | 2011-10-18 | Promega Corporation | Methods of purifying a nucleic acid and formulation and kit for use in performing such methods |
EP2847330B1 (en) * | 2012-05-09 | 2018-04-18 | Bio-Rad Laboratories, Inc. | Buffer for one-step dna extraction |
RU2648983C1 (ru) | 2014-09-24 | 2018-03-29 | Басф Се | Способ получения сложных диэфиров терефталевой кислоты |
BR112017005850A2 (pt) | 2014-09-24 | 2018-02-06 | Basf Se | processo e aparelho para preparação de um diéster tereftálico. |
TR201904928T4 (tr) | 2014-09-24 | 2019-05-21 | Basf Se | Tereftalik asit diesterlerin resirküle alkol dehidratasyonu ile üretimi için yöntem. |
RU2668964C1 (ru) | 2014-09-24 | 2018-10-05 | Басф Се | Способ получения сложных диэфиров терефталевой кислоты с перекачиванием реакционной смеси |
DE102016106271B4 (de) | 2016-04-06 | 2018-03-29 | Axagarius Gmbh & Co. Kg | Aufreinigung von Nukleinsäure aus einer Nukleinsäure und Endotoxin enthaltenden Probe |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4935342A (en) † | 1986-12-01 | 1990-06-19 | Syngene, Inc. | Method of isolating and purifying nucleic acids from biological samples |
CA2067711C (en) * | 1991-05-03 | 2000-08-08 | Daniel Lee Woodard | Solid phase extraction purification of dna |
CA2067712A1 (en) * | 1991-05-03 | 1992-11-04 | Daniel L. Woodard | Filtration purification of dna |
DE4139664A1 (de) * | 1991-12-02 | 1993-06-03 | Diagen Inst Molekularbio | Vorrichtung und verfahren zur isolierung und reinigung von nukleinsaeuren |
DE4321904B4 (de) * | 1993-07-01 | 2013-05-16 | Qiagen Gmbh | Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen |
-
1995
- 1995-02-03 AT AT95907640T patent/ATE181921T1/de active
- 1995-02-03 WO PCT/EP1995/000390 patent/WO1995021178A1/de active IP Right Grant
- 1995-02-03 DK DK95907640T patent/DK0743948T4/da active
- 1995-02-03 JP JP52039095A patent/JP3379758B2/ja not_active Expired - Lifetime
- 1995-02-03 CA CA002182398A patent/CA2182398C/en not_active Expired - Lifetime
- 1995-02-03 EP EP95907640A patent/EP0743948B2/de not_active Expired - Lifetime
- 1995-02-03 AU AU15776/95A patent/AU684134B2/en not_active Expired
- 1995-02-03 US US08/687,530 patent/US5792651A/en not_active Expired - Lifetime
- 1995-02-03 DE DE59506355T patent/DE59506355D1/de not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
AU1577695A (en) | 1995-08-21 |
AU684134B2 (en) | 1997-12-04 |
DE59506355D1 (de) | 1999-08-12 |
EP0743948B2 (de) | 2003-10-08 |
DK0743948T3 (da) | 2000-01-31 |
EP0743948A1 (de) | 1996-11-27 |
EP0743948B1 (de) | 1999-07-07 |
CA2182398C (en) | 2003-01-07 |
US5792651A (en) | 1998-08-11 |
JPH09508283A (ja) | 1997-08-26 |
WO1995021178A1 (de) | 1995-08-10 |
ATE181921T1 (de) | 1999-07-15 |
CA2182398A1 (en) | 1995-08-10 |
JP3379758B2 (ja) | 2003-02-24 |
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