DK0627954T3 - Fremgangsmåde til elektroeluering af en gel indeholdende adskilte ladede makromolekyler såsom proteiner eller DNA/RNA og et apparat og midler til brug ved fremgangsmåden - Google Patents
Fremgangsmåde til elektroeluering af en gel indeholdende adskilte ladede makromolekyler såsom proteiner eller DNA/RNA og et apparat og midler til brug ved fremgangsmådenInfo
- Publication number
- DK0627954T3 DK0627954T3 DK92905561.4T DK92905561T DK0627954T3 DK 0627954 T3 DK0627954 T3 DK 0627954T3 DK 92905561 T DK92905561 T DK 92905561T DK 0627954 T3 DK0627954 T3 DK 0627954T3
- Authority
- DK
- Denmark
- Prior art keywords
- electroeluting
- rna
- dna
- proteins
- gel containing
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/44739—Collecting the separated zones, e.g. blotting to a membrane or punching of gel spots
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Saccharide Compounds (AREA)
- Electrostatic Separation (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA002130751A CA2130751C (en) | 1992-02-25 | 1992-02-25 | Apparatus and process for electroelution of a gel containing charged macromolecules |
EP92905561A EP0627954B1 (en) | 1992-02-25 | 1992-02-25 | Process for electroelution of a gel containing separated charged macromolecules, such as proteins or dna/rna, and an apparatus and means for use in the process |
PCT/DK1992/000058 WO1993016788A1 (en) | 1992-02-25 | 1992-02-25 | Process for electroelution of a gel containing separated charged macromolecules, such as proteins or dna/rna, and an apparatus and means for use in the process |
Publications (1)
Publication Number | Publication Date |
---|---|
DK0627954T3 true DK0627954T3 (da) | 1996-08-26 |
Family
ID=25677447
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK92905561.4T DK0627954T3 (da) | 1992-02-25 | 1992-02-25 | Fremgangsmåde til elektroeluering af en gel indeholdende adskilte ladede makromolekyler såsom proteiner eller DNA/RNA og et apparat og midler til brug ved fremgangsmåden |
Country Status (7)
Country | Link |
---|---|
US (1) | US5840169A (da) |
EP (1) | EP0627954B1 (da) |
AU (1) | AU1324192A (da) |
CA (1) | CA2130751C (da) |
DE (1) | DE69210048T2 (da) |
DK (1) | DK0627954T3 (da) |
WO (1) | WO1993016788A1 (da) |
Families Citing this family (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE19939730C2 (de) * | 1999-08-21 | 2003-04-30 | Wilhelm Breuer | Vorrichtung zur Verwendung bei der Elektroelution von Makromolekülen und ein Verfahren zur Elektroelution von Makromolekülen aus Gelen unter Minimierung des Elutionsvolumens |
US6602391B2 (en) | 2001-03-05 | 2003-08-05 | Vladimir B. Serikov | Apparatus and method for combined capillary separation and blotting of biological macromolecules |
WO2003033740A2 (en) * | 2001-07-10 | 2003-04-24 | Massachusetts Institute Of Technology | Apparatus and method for isolating a nucleic acid |
WO2003016896A1 (en) * | 2001-08-10 | 2003-02-27 | Flesher Robert W | A method and device for electrophoresis and blotting |
DE10149875A1 (de) * | 2001-10-10 | 2003-07-03 | Alpha Technology Ges Fuer Ange | Vorrichtung zur Extraktion elektrisch geladener Moleküle |
US20040126783A1 (en) * | 2002-07-10 | 2004-07-01 | Massachusetts Institute Of Technology | Apparatus and method for isolating a nucleic acid from a sample |
US7250100B2 (en) * | 2003-06-17 | 2007-07-31 | Duke University | Two dimensional electrophoresis cassette |
WO2006136296A1 (en) * | 2005-06-18 | 2006-12-28 | Ge Healthcare Bio-Sciences Ab | Method and devices for forming a plurality of wells on a gel |
CA2612056A1 (en) * | 2005-06-18 | 2006-12-28 | Ge Healthcare Bio-Sciences Ab | Methods and systems for adding a reagent to an analyte in a gel |
US7528368B2 (en) * | 2006-03-23 | 2009-05-05 | Protea Biosciences, Inc. | Electrospray ionization process and add-on device with sample injection tip |
US7641778B2 (en) * | 2006-03-23 | 2010-01-05 | Protea Biosciences, Inc. | Gel electroelution and sample separation devices and associated processes |
US20080296158A1 (en) | 2007-05-31 | 2008-12-04 | Sharp Kabushiki Kaisha | Device for electrophoresis, device for transfer, device for electrophoresis and transfer, chip for electrophoresis and transfer, and method for electrophoresis, method for transfer, and method for electrophoresis and transfer |
US20090250345A1 (en) * | 2008-04-03 | 2009-10-08 | Protea Biosciences, Inc. | Microfluidic electroelution devices & processes |
US20090250347A1 (en) * | 2008-04-03 | 2009-10-08 | Protea Biosciences, Inc. | Microfluidic devices & processes for electrokinetic transport |
US8361299B2 (en) | 2008-10-08 | 2013-01-29 | Sage Science, Inc. | Multichannel preparative electrophoresis system |
WO2010042766A1 (en) * | 2008-10-08 | 2010-04-15 | Sage Science, Inc. | Multichannel preparative electrophoresis system |
CN107144622A (zh) * | 2011-06-16 | 2017-09-08 | 不列颠哥伦比亚癌症分社 | 核酸的自动化尺寸选择 |
AU2013329110B2 (en) | 2012-10-12 | 2017-05-04 | Sage Science, Inc. | Side-eluting molecular fractionator |
JP6612340B2 (ja) | 2014-10-15 | 2019-11-27 | セージ サイエンス, インコーポレイテッド | 核酸の自動化された加工処理および電気泳動による試料調製のための装置、方法およびシステム |
US11542495B2 (en) | 2015-11-20 | 2023-01-03 | Sage Science, Inc. | Preparative electrophoretic method for targeted purification of genomic DNA fragments |
AU2018250330A1 (en) | 2017-04-07 | 2019-09-19 | Sage Science, Inc. | Systems and methods for detection of genetic structural variation using integrated electrophoretic DNA purification |
WO2023164488A1 (en) * | 2022-02-25 | 2023-08-31 | Life Technologies Corporation | Biomolecule transfer system and methods |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3956099A (en) * | 1972-10-30 | 1976-05-11 | Louis Israel | Continuous preparative electrophoresis apparatus |
US4049534A (en) * | 1976-04-12 | 1977-09-20 | E-C Apparatus Corporation | Electrophorectic apparatus for elution from preparative gels |
US4181594A (en) * | 1979-03-27 | 1980-01-01 | University Of Pittsburgh | Matrix recovery electrophoresis apparatus |
DE3337669C2 (de) * | 1983-10-17 | 1989-09-21 | Carl Schleicher & Schuell Gmbh & Co Kg, 3352 Einbeck | Gerät zur Elektroelution elektrisch geladener Makromoleküle |
GB8331514D0 (en) * | 1983-11-25 | 1984-01-04 | Janssen Pharmaceutica Nv | Visualization method |
DE3417180C1 (de) * | 1984-05-09 | 1985-05-15 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V., 3400 Göttingen | Vorrichtung zum Eluieren von elektrisch geladenen Makromolekuelen,insbesondere Biopolymeren |
US4747918A (en) * | 1986-06-18 | 1988-05-31 | William Bellomy | Electroelution apparatus and method of using same |
DE3829111A1 (de) * | 1988-08-27 | 1990-03-01 | Hoechst Ag | Verfahren und vorrichtung zum elektrophoretischen trennen, reinigen und anreichern von geladenen oder polarisierbaren makromolekuelen |
US4959133A (en) * | 1989-01-23 | 1990-09-25 | Eastman Kodak Company | Field inversion electroblotting & electroelution |
-
1992
- 1992-02-25 CA CA002130751A patent/CA2130751C/en not_active Expired - Fee Related
- 1992-02-25 EP EP92905561A patent/EP0627954B1/en not_active Expired - Lifetime
- 1992-02-25 US US08/290,993 patent/US5840169A/en not_active Expired - Lifetime
- 1992-02-25 DK DK92905561.4T patent/DK0627954T3/da active
- 1992-02-25 DE DE69210048T patent/DE69210048T2/de not_active Expired - Lifetime
- 1992-02-25 WO PCT/DK1992/000058 patent/WO1993016788A1/en active IP Right Grant
- 1992-02-25 AU AU13241/92A patent/AU1324192A/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
WO1993016788A1 (en) | 1993-09-02 |
CA2130751A1 (en) | 1993-08-26 |
DE69210048D1 (de) | 1996-05-23 |
DE69210048T2 (de) | 1996-08-29 |
AU1324192A (en) | 1993-09-13 |
EP0627954A1 (en) | 1994-12-14 |
CA2130751C (en) | 2000-01-25 |
EP0627954B1 (en) | 1996-04-17 |
US5840169A (en) | 1998-11-24 |
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