DK0530447T3 - Method for purifying IgG monoclonal antibodies - Google Patents

Method for purifying IgG monoclonal antibodies

Info

Publication number
DK0530447T3
DK0530447T3 DK92108912.4T DK92108912T DK0530447T3 DK 0530447 T3 DK0530447 T3 DK 0530447T3 DK 92108912 T DK92108912 T DK 92108912T DK 0530447 T3 DK0530447 T3 DK 0530447T3
Authority
DK
Denmark
Prior art keywords
monoclonal antibodies
igg monoclonal
purifying igg
purifying
immunotherapy
Prior art date
Application number
DK92108912.4T
Other languages
Danish (da)
Inventor
Norbert Dr Kothe
Dieter Dr Rudnick
Ulf Dr Bethke
Michael Dr Kloft
Detlef Dr Rohm
Original Assignee
Biotest Pharma Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biotest Pharma Gmbh filed Critical Biotest Pharma Gmbh
Application granted granted Critical
Publication of DK0530447T3 publication Critical patent/DK0530447T3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

The present invention relates to a process for purifying IgG monoclonal antibodies by a combination of three chromatography steps, namely on an anion exchanger, on a cation exchanger and on a hydrophobic phase with a specific sterilisation step. This results in an antibody solution with a purity of at least 99.5%, which can thus be used intravenously in immunotherapy.
DK92108912.4T 1991-06-08 1992-05-27 Method for purifying IgG monoclonal antibodies DK0530447T3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE4118912A DE4118912C1 (en) 1991-06-08 1991-06-08

Publications (1)

Publication Number Publication Date
DK0530447T3 true DK0530447T3 (en) 1998-01-12

Family

ID=6433511

Family Applications (1)

Application Number Title Priority Date Filing Date
DK92108912.4T DK0530447T3 (en) 1991-06-08 1992-05-27 Method for purifying IgG monoclonal antibodies

Country Status (7)

Country Link
EP (1) EP0530447B1 (en)
JP (1) JP2644946B2 (en)
AT (1) ATE159532T1 (en)
DE (2) DE4118912C1 (en)
DK (1) DK0530447T3 (en)
ES (1) ES2108059T3 (en)
GR (1) GR3025603T3 (en)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2706466B1 (en) * 1993-06-14 1995-08-25 Aetsrn Immunoglobulin G concentrate for therapeutic use and process for producing said concentrate.
IL121900A (en) * 1997-10-07 2001-12-23 Omrix Biopharmaceuticals Ltd Method for the purification of immunoglobulins
PL196770B1 (en) * 1998-06-09 2008-01-31 Statens Seruminstitut Process for producing immunoglobulins for intravenous administration and other immunoglobulin products
US6281336B1 (en) 1998-06-09 2001-08-28 Statens Serum Institut Process for producing immunoglobulins for intravenous administration and other immunoglobulin products
SE0001128D0 (en) * 2000-03-30 2000-03-30 Amersham Pharm Biotech Ab A method of producing IgG
US20060257972A1 (en) * 2003-03-31 2006-11-16 Takashi Ishihara Purification of human monoclonal antibody and human polyclonal antibody
EP1532983A1 (en) 2003-11-18 2005-05-25 ZLB Bioplasma AG Immunoglobulin preparations having increased stability
TWI391399B (en) 2005-05-25 2013-04-01 Hoffmann La Roche Method for determining the concentration of a salt for eluting a polypeptide
CA2687837A1 (en) 2007-06-01 2008-12-04 F. Hoffmann-La Roche Ag Immunoglobulin purification
AR067536A1 (en) 2007-07-17 2009-10-14 Hoffmann La Roche METHOD FOR OBTAINING A MONO-PEGILATED ERYTHROPOYETIN IN A SUBSTANTIALLY HOMOGENOUS FORM
AR067537A1 (en) 2007-07-17 2009-10-14 Hoffmann La Roche PURIFIED POLYPEPTIDES PURIFICATION
WO2009058769A1 (en) * 2007-10-30 2009-05-07 Schering Corporation Purification of antibodies containing hydrophobic variants
JP2012513425A (en) 2008-12-22 2012-06-14 エフ．ホフマン−ラ ロシュ アーゲー Immunoglobulin purification
WO2011095543A1 (en) 2010-02-04 2011-08-11 Csl Behring Ag Immunoglobulin preparation
CA2787897A1 (en) * 2010-02-12 2011-08-18 Dsm Ip Assets B.V. Single unit antibody purification
EP2361636A1 (en) 2010-02-26 2011-08-31 CSL Behring AG Immunoglobulin preparation and storage system for an immunoglobulin preparation
JP5735650B2 (en) 2010-09-14 2015-06-17 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ Method for purifying PEGylated erythropoietin
EP2635599A1 (en) * 2010-11-01 2013-09-11 DSM IP Assets B.V. Single unit ion exchange chromatography antibody purification
US20130053551A1 (en) 2011-08-25 2013-02-28 Hoffman-La Roche. Inc. Cation and anion exchange chromatography method
ES2546064T3 (en) 2011-11-22 2015-09-18 Heraeus Medical Gmbh Sterilization of polymerizable monomers
WO2015137530A1 (en) * 2014-03-11 2015-09-17 주식회사 녹십자홀딩스 Method for purifying immunoglobulin
CN109415427A (en) 2016-07-15 2019-03-01 豪夫迈·罗氏有限公司 Method for purifying the hematopoietin of Pegylation

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4469630A (en) * 1983-11-25 1984-09-04 J. T. Baker Chemical Co. Purification of monoclonal antibodies
US4743680A (en) * 1985-02-01 1988-05-10 New York University Method for purifying antihemophilic factor
DE3640513A1 (en) * 1986-11-27 1988-06-09 Biotest Pharma Gmbh METHOD FOR THE PRODUCTION OF A VIRUS-SAFE, STORAGE-STABLE AND INTRAVENOES COMPATIBLE IMMUNGLOBULIN-G-PRAEPARATES
DE3641115A1 (en) * 1986-12-02 1988-06-16 Lentia Gmbh METHOD FOR PRODUCING AN INTRAVENOUS APPLICABLE AND STABLE IN LIQUID FORM IMMUNOGLOBULINS
CA1323567C (en) * 1987-10-05 1993-10-26 Gene R. Nathans Method for purification of antibodies
US5118796A (en) * 1987-12-09 1992-06-02 Centocor, Incorporated Efficient large-scale purification of immunoglobulins and derivatives
CA2005600A1 (en) * 1988-12-20 1990-06-20 Cal-Henrik Heldin Endothelial cell growth factor

Also Published As

Publication number Publication date
ES2108059T3 (en) 1997-12-16
EP0530447A2 (en) 1993-03-10
EP0530447B1 (en) 1997-10-22
DE59208982D1 (en) 1997-11-27
JP2644946B2 (en) 1997-08-25
DE4118912C1 (en) 1992-07-02
JPH07267997A (en) 1995-10-17
GR3025603T3 (en) 1998-03-31
ATE159532T1 (en) 1997-11-15
EP0530447A3 (en) 1993-08-18

Similar Documents

Publication Publication Date Title
DK0530447T3 (en) Method for purifying IgG monoclonal antibodies
DK0821695T3 (en) Purification of antibody by hydrophobic interaction chromatography at low pH
WO1990010709A3 (en) Monoclonal antibodies for metallic cations on small molecules
CA2121257A1 (en) Stabilised Antibodies
DK0538754T3 (en) Use of antibody-containing preparations for immunosuppression
AU558931B2 (en) A process and apparatus for the recovery of immunoglobulins
NZ509135A (en) A third generation intravenous immunoglobulin G (IVIG) purified using column chromatographic ion exchange methods
BR9804826A (en) Antigenic fusion protein carrying galoc epitopes 1.3 gal
MX9603637A (en) Antibody purification.
ES8506902A1 (en) Method of affinity purification employing monoclonal antibodies.
Saluk et al. The unique molecular weight of the heavy chain from human IgG3
EP0282317A3 (en) Purified platelet-derived growth factor and methods for purification thereof
DK407987D0 (en) APOLIPOPROTEIN B-SPECIFIC MONOCLONAL ANTIBODIES AND HYBRIDOMES PRODUCING THESE ANTIBODIES
GB8910263D0 (en) Monoclonal antibodies specific for an immunoglobulin isotype
ES2114547T3 (en) MONOCLONAL ANTIBODIES AGAINST THE HEPATITIS C VIRUS.
DK0597358T3 (en) Process for Quantitative Purification of Glycolipids
AU2124692A (en) A process for the purification of factor xiii, monoclonal antibodies against factor xiiia, the preparation and use thereof
FI860937A (en) Method and reagent for the determination of follicle-stimulating hormone and suitable monoclonal antibodies
EP0539584A4 (en)
DK0513565T3 (en) Monoclonal antibodies against the plasmin-antiplasmin complex, method for their preparation and their use
IT1237997B (en) THIOPHILIC ABSORBENTS
ATE193021T1 (en) LIPOPROTEIN (A) PEPTIDES AND THEIR USE