DK0506889T3 - Reverse transkriptaser ved høj temperatur - Google Patents

Reverse transkriptaser ved høj temperatur

Info

Publication number
DK0506889T3
DK0506889T3 DK91904087.3T DK91904087T DK0506889T3 DK 0506889 T3 DK0506889 T3 DK 0506889T3 DK 91904087 T DK91904087 T DK 91904087T DK 0506889 T3 DK0506889 T3 DK 0506889T3
Authority
DK
Denmark
Prior art keywords
high temperature
reverse transcriptases
reverse transcription
rna
amplification
Prior art date
Application number
DK91904087.3T
Other languages
Danish (da)
English (en)
Inventor
David H Gelfand
Thomas W Myers
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27412639&utm_source=***_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK0506889(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from US07/455,611 external-priority patent/US5322770A/en
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of DK0506889T3 publication Critical patent/DK0506889T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK91904087.3T 1989-12-22 1990-12-21 Reverse transkriptaser ved høj temperatur DK0506889T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US45596789A 1989-12-22 1989-12-22
US07/455,611 US5322770A (en) 1989-12-22 1989-12-22 Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
US58547190A 1990-09-20 1990-09-20

Publications (1)

Publication Number Publication Date
DK0506889T3 true DK0506889T3 (da) 1997-09-22

Family

ID=27412639

Family Applications (1)

Application Number Title Priority Date Filing Date
DK91904087.3T DK0506889T3 (da) 1989-12-22 1990-12-21 Reverse transkriptaser ved høj temperatur

Country Status (10)

Country Link
EP (1) EP0506889B1 (fr)
JP (1) JP2968585B2 (fr)
AT (1) ATE151112T1 (fr)
AU (1) AU656315B2 (fr)
CA (1) CA2071213C (fr)
DE (1) DE69030386T2 (fr)
DK (1) DK0506889T3 (fr)
ES (1) ES2100945T3 (fr)
GR (1) GR3023862T3 (fr)
WO (1) WO1991009944A2 (fr)

Families Citing this family (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5466591A (en) * 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5693517A (en) * 1987-06-17 1997-12-02 Roche Molecular Systems, Inc. Reagents and methods for coupled high temperature reverse transcription and polymerase chain reactions
US5795762A (en) * 1986-08-22 1998-08-18 Roche Molecular Systems, Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5561058A (en) * 1986-08-22 1996-10-01 Hoffmann-La Roche Inc. Methods for coupled high temperatures reverse transcription and polymerase chain reactions
US5374553A (en) * 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
EP0550687B1 (fr) * 1990-09-28 1999-06-09 F. Hoffmann-La Roche Ag Mutations d'adn-polymerases thermostables en 5' a 3' exonuclease
JPH06502303A (ja) * 1990-10-05 1994-03-17 バーネス，ウェーン・エム 熱安定性ｄｎａポリメラーゼ
CA2038839A1 (fr) * 1991-03-22 1992-09-23 Jiang J. Liu Methode de detection de l'adn viral
AU662906B2 (en) * 1991-06-26 1995-09-21 F. Hoffmann-La Roche Ag Methods for detection of carcinoma metastases by nucleic acid amplification
DE69223562T2 (de) * 1991-08-27 1998-06-04 Hoffmann La Roche Verfahren und Reagenzien zum Nachweis von Hepatitis C
FR2690691B1 (fr) * 1992-04-29 1999-02-12 Bio Merieux Procede d'amplification d'arn necessitant une seule etape de manipulation.
USRE39031E1 (en) * 1992-04-29 2006-03-21 Biomerieux RNA amplification method requiring only one manipulation step
DK0632134T3 (da) * 1993-07-01 2001-12-03 Hoffmann La Roche Reagenser og fremgangsmåder til koblet revers transkription ved høj temperatur og polymerase-kædereaktioner
US5474920A (en) * 1993-11-23 1995-12-12 State Of Oregon, Acting By And Through The Oregon State Board Of Higher Education On Behalf Of The Oregon Health Sciences University Modified thermo-resistant DNA polymerases
EP1251182A3 (fr) 1993-12-01 2002-11-27 Toyo Boseki Kabushiki Kaisha Procédé d'amplification et de détection d'une séquence nucléotidique au moyen d'enzymes thermostables
US5648211A (en) * 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
EP0736609A3 (fr) * 1995-04-08 1998-07-29 Roche Diagnostics GmbH Procédé pour l'amplification spécifique et pour la détection d'ADN ou d'ARN
CA2222744C (fr) * 1995-05-31 2008-03-25 Amersham Life Science, Inc. Adn-polymerases thermostables
US6077664A (en) * 1995-06-07 2000-06-20 Promega Corporation Thermophilic DNA polymerases from Thermotoga neapolitana
US6001645A (en) * 1995-06-07 1999-12-14 Promega Corporation Thermophilic DNA polymerases from thermotoga neapolitana
ATE203056T1 (de) * 1995-12-15 2001-07-15 Amersham Life Science Inc Thermostabile dna polymerase aus thermoanaerobacter thermohydrosulfuricus und davon abgeleitete mutierte enzyme mit entfernter exonukleaseaktivität
CA2199213C (fr) * 1996-03-11 2007-04-17 John Wesley Backus Amplification et detection des acides nucleiques cibles grace a une etape d'incubation post-amplification
US6013488A (en) * 1996-07-25 2000-01-11 The Institute Of Physical And Chemical Research Method for reverse transcription
ES2121698B1 (es) * 1996-12-02 1999-07-01 Inia Procedimiento para la obtencionn de fragmentos amplificados de acido nucleico de particulas virales y subvirales que contienen rna.
US6030814A (en) * 1997-04-21 2000-02-29 Epicentre Technologies Corporation Reverse transcription method
ES2163271T3 (es) * 1997-05-13 2002-01-16 Azign Bioscience As Procedimiento para clonar arnm y visualizar transcriptores diferencialmente expresados (dodets).
EP0921196A1 (fr) * 1997-12-02 1999-06-09 Roche Diagnostics GmbH Polymérase d'ADN modifiée de Carboxydothermus hydrogenoformans et son utilisation pour transcription inverse suivi par la réaction en chaíne de la polymérase
EP0922765B1 (fr) * 1997-12-02 2005-11-23 Roche Diagnostics GmbH Polymérase d'ADN modifiée de Carboxydothermus hydrogenoformans et son utilisation pour transcription inverse suivi par la réaction en chaíne de la polymérase
WO1999047539A1 (fr) * 1998-03-18 1999-09-23 Amersham Pharmacia Biotech, Inc. Polymerase d'adn thermostable provenant du thermoanaerobacter thermohydrosulfuricus
AT410218B (de) 1999-08-20 2003-03-25 Baxter Ag Verfahren zur herstellung eines qualitätsgesicherten pools biologischer proben
US6436677B1 (en) 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
US6632645B1 (en) 2000-03-02 2003-10-14 Promega Corporation Thermophilic DNA polymerases from Thermoactinomyces vulgaris
KR20000072141A (ko) * 2000-08-04 2000-12-05 김유삼 신규한 호열성 단백질 분해효소의 ｄｎａ 서열 및그로부터 유래된 단백질
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
US7575864B2 (en) * 2004-05-27 2009-08-18 E.I. Du Pont De Nemours And Company Method for the direct detection of diagnostic RNA
US7504240B2 (en) * 2005-03-10 2009-03-17 Masanori Hirano Methods for synthesizing polynucleotides using a single primer
JP5191041B2 (ja) 2007-04-05 2013-04-24 エフ．ホフマン−ラ ロシュ アーゲー 急速ワンステップｒｔ−ｐｃｒ
KR101039521B1 (ko) * 2008-02-01 2011-06-08 주식회사 인트론바이오테크놀로지 금속 이온에 의해 발생하는 ｐｃｒ 저해 및 비특이 증폭을완화시키거나 막을 수 있는 에틸렌글리콜테트라아세틱 산을킬레이트제로 포함하는 ｐｃｒ 반응용 조성물
US9045729B2 (en) 2009-12-10 2015-06-02 Ottawa Hospital Research Institute Oncolytic rhabdovirus
US9364532B2 (en) 2011-06-08 2016-06-14 Children's Hospital Of Eastern Ontario Research Institute Inc. Compositions and methods for glioblastoma treatment
WO2013185081A1 (fr) * 2012-06-08 2013-12-12 Ionian Technologies, Inc Amplifications d'acide nucléique
EP3622965A1 (fr) 2013-02-21 2020-03-18 Turnstone Limited Partnership Composition de vaccin
EP3099810B1 (fr) 2014-01-31 2019-05-01 Qiagen GmbH Démarrage à chaud pour chélateur cationique
US10301660B2 (en) 2015-03-30 2019-05-28 Takara Bio Usa, Inc. Methods and compositions for repair of DNA ends by multiple enzymatic activities
US11236384B2 (en) 2017-01-19 2022-02-01 Asuragen, Inc. Methods of RNA amplification
GB201812192D0 (en) 2018-07-26 2018-09-12 Ttp Plc Variable temperature reactor, heater and control circuit for the same
CN110846724B (zh) * 2019-12-03 2023-04-28 江西海普洛斯医学检验实验室有限公司 构建mRNA链特异文库的方法及试剂盒
CN113045410A (zh) * 2021-03-29 2021-06-29 华东理工大学 一种双环降二萜类化合物及其合成基因及制备方法
EP4074839A1 (fr) 2021-04-16 2022-10-19 Biotype GmbH Sonde tx oligonucléotidique optimisée pour une analyse de multiplexage d'acides nucléiques et méthode de multiplexage
EP4350002A1 (fr) 2022-10-07 2024-04-10 Biotype GmbH Detection of molecular analytes based on tailored probe competition

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1338457C (fr) * 1986-08-22 1996-07-16 Henry A. Erlich Enzyme thermostable purifiee

Also Published As

Publication number Publication date
GR3023862T3 (en) 1997-09-30
DE69030386D1 (de) 1997-05-07
EP0506889B1 (fr) 1997-04-02
WO1991009944A3 (fr) 1991-08-22
JP2968585B2 (ja) 1999-10-25
CA2071213C (fr) 2000-05-02
DE69030386T2 (de) 1997-10-09
ATE151112T1 (de) 1997-04-15
CA2071213A1 (fr) 1991-06-23
EP0506889A1 (fr) 1992-10-07
AU7244491A (en) 1991-07-24
JPH05505105A (ja) 1993-08-05
ES2100945T3 (es) 1997-07-01
WO1991009944A2 (fr) 1991-07-11
AU656315B2 (en) 1995-02-02

Similar Documents

Publication Publication Date Title
DK0506889T3 (da) Reverse transkriptaser ved høj temperatur
US6399320B1 (en) Modified DNA-polymerase from carboxydothermus hydrogenoformans and its use for coupled reverse transcription and polymerase chain reaction
EP0929680B1 (fr) Polymerase d'adn thermostable provenant de carboxydothermus hydrogenoformans
CN103987843B (zh) 具有改进活性的dna聚合酶
NO931709L (no) Nukleinsyreamplifisering ved hjelp av to-enzymers, selvunderholdende sekvensreplikasjon
EP2272952B8 (fr) ADN polymérase thermostable ou thermoactif ayant une activité 3' - 5' exonucléase atténuée
EP0428197A3 (fr) Méthodes pour l'extraction des acides nucléiques et pour les amplifier par PCR sans utiliser un enzyme protéolytique
CN103987844B (zh) 具有改进活性的dna聚合酶
DE69426400D1 (de) Thermostable Nukleinsäure Polymerase
AU3062989A (en) Purified thermostable enzyme
DK0669401T3 (da) Amplificering af lange nukleinsyresekvenser ved PCR
JPH05506364A (ja) 熱安定性　ｄｎａポリメラーゼの５→３′のエキソヌクレアーゼ突然変異
DK1705253T3 (da) Sammensætninger og fremgangsmåder til revers transkriptase-polymerasekædereaktion (RT-PCR)
CN103687943B (zh) 具有改进活性的dna聚合酶
CA2127188A1 (fr) Reactifs et methodes pour coupler la transcription inverse a haute temperature et les reactions d'amplification par la polymerase
EP0272098A3 (fr) Procédé d'amplification et de détection de séquences ARN
DE69737024D1 (en) Thermostabiles nukleinsäure-polymerase von thermococcus gorgonarius
US6692932B1 (en) Thermostable DNA polymerase from anaerocellum thermophilum
RU2221867C2 (ru) Очищенная термостабильная днк-полимераза из thermococcus gorgonarius, ее получение и применение
Gelfand et al. Reverse transcription with thermus thermophilus polymerase
ATE367452T1 (de) Methode zur reversen transkription von im getrockneten zustand isolierter rna