DK0506889T3 - Reverse transkriptaser ved høj temperatur - Google Patents
Reverse transkriptaser ved høj temperaturInfo
- Publication number
- DK0506889T3 DK0506889T3 DK91904087.3T DK91904087T DK0506889T3 DK 0506889 T3 DK0506889 T3 DK 0506889T3 DK 91904087 T DK91904087 T DK 91904087T DK 0506889 T3 DK0506889 T3 DK 0506889T3
- Authority
- DK
- Denmark
- Prior art keywords
- high temperature
- reverse transcriptases
- reverse transcription
- rna
- amplification
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US45596789A | 1989-12-22 | 1989-12-22 | |
US07/455,611 US5322770A (en) | 1989-12-22 | 1989-12-22 | Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription |
US58547190A | 1990-09-20 | 1990-09-20 |
Publications (1)
Publication Number | Publication Date |
---|---|
DK0506889T3 true DK0506889T3 (da) | 1997-09-22 |
Family
ID=27412639
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK91904087.3T DK0506889T3 (da) | 1989-12-22 | 1990-12-21 | Reverse transkriptaser ved høj temperatur |
Country Status (10)
Country | Link |
---|---|
EP (1) | EP0506889B1 (fr) |
JP (1) | JP2968585B2 (fr) |
AT (1) | ATE151112T1 (fr) |
AU (1) | AU656315B2 (fr) |
CA (1) | CA2071213C (fr) |
DE (1) | DE69030386T2 (fr) |
DK (1) | DK0506889T3 (fr) |
ES (1) | ES2100945T3 (fr) |
GR (1) | GR3023862T3 (fr) |
WO (1) | WO1991009944A2 (fr) |
Families Citing this family (50)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5466591A (en) * | 1986-08-22 | 1995-11-14 | Hoffmann-La Roche Inc. | 5' to 3' exonuclease mutations of thermostable DNA polymerases |
US5693517A (en) * | 1987-06-17 | 1997-12-02 | Roche Molecular Systems, Inc. | Reagents and methods for coupled high temperature reverse transcription and polymerase chain reactions |
US5795762A (en) * | 1986-08-22 | 1998-08-18 | Roche Molecular Systems, Inc. | 5' to 3' exonuclease mutations of thermostable DNA polymerases |
US5561058A (en) * | 1986-08-22 | 1996-10-01 | Hoffmann-La Roche Inc. | Methods for coupled high temperatures reverse transcription and polymerase chain reactions |
US5374553A (en) * | 1986-08-22 | 1994-12-20 | Hoffmann-La Roche Inc. | DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima |
EP0550687B1 (fr) * | 1990-09-28 | 1999-06-09 | F. Hoffmann-La Roche Ag | Mutations d'adn-polymerases thermostables en 5' a 3' exonuclease |
JPH06502303A (ja) * | 1990-10-05 | 1994-03-17 | バーネス,ウェーン・エム | 熱安定性dnaポリメラーゼ |
CA2038839A1 (fr) * | 1991-03-22 | 1992-09-23 | Jiang J. Liu | Methode de detection de l'adn viral |
AU662906B2 (en) * | 1991-06-26 | 1995-09-21 | F. Hoffmann-La Roche Ag | Methods for detection of carcinoma metastases by nucleic acid amplification |
DE69223562T2 (de) * | 1991-08-27 | 1998-06-04 | Hoffmann La Roche | Verfahren und Reagenzien zum Nachweis von Hepatitis C |
FR2690691B1 (fr) * | 1992-04-29 | 1999-02-12 | Bio Merieux | Procede d'amplification d'arn necessitant une seule etape de manipulation. |
USRE39031E1 (en) * | 1992-04-29 | 2006-03-21 | Biomerieux | RNA amplification method requiring only one manipulation step |
DK0632134T3 (da) * | 1993-07-01 | 2001-12-03 | Hoffmann La Roche | Reagenser og fremgangsmåder til koblet revers transkription ved høj temperatur og polymerase-kædereaktioner |
US5474920A (en) * | 1993-11-23 | 1995-12-12 | State Of Oregon, Acting By And Through The Oregon State Board Of Higher Education On Behalf Of The Oregon Health Sciences University | Modified thermo-resistant DNA polymerases |
EP1251182A3 (fr) | 1993-12-01 | 2002-11-27 | Toyo Boseki Kabushiki Kaisha | Procédé d'amplification et de détection d'une séquence nucléotidique au moyen d'enzymes thermostables |
US5648211A (en) * | 1994-04-18 | 1997-07-15 | Becton, Dickinson And Company | Strand displacement amplification using thermophilic enzymes |
EP0736609A3 (fr) * | 1995-04-08 | 1998-07-29 | Roche Diagnostics GmbH | Procédé pour l'amplification spécifique et pour la détection d'ADN ou d'ARN |
CA2222744C (fr) * | 1995-05-31 | 2008-03-25 | Amersham Life Science, Inc. | Adn-polymerases thermostables |
US6077664A (en) * | 1995-06-07 | 2000-06-20 | Promega Corporation | Thermophilic DNA polymerases from Thermotoga neapolitana |
US6001645A (en) * | 1995-06-07 | 1999-12-14 | Promega Corporation | Thermophilic DNA polymerases from thermotoga neapolitana |
ATE203056T1 (de) * | 1995-12-15 | 2001-07-15 | Amersham Life Science Inc | Thermostabile dna polymerase aus thermoanaerobacter thermohydrosulfuricus und davon abgeleitete mutierte enzyme mit entfernter exonukleaseaktivität |
CA2199213C (fr) * | 1996-03-11 | 2007-04-17 | John Wesley Backus | Amplification et detection des acides nucleiques cibles grace a une etape d'incubation post-amplification |
US6013488A (en) * | 1996-07-25 | 2000-01-11 | The Institute Of Physical And Chemical Research | Method for reverse transcription |
ES2121698B1 (es) * | 1996-12-02 | 1999-07-01 | Inia | Procedimiento para la obtencionn de fragmentos amplificados de acido nucleico de particulas virales y subvirales que contienen rna. |
US6030814A (en) * | 1997-04-21 | 2000-02-29 | Epicentre Technologies Corporation | Reverse transcription method |
ES2163271T3 (es) * | 1997-05-13 | 2002-01-16 | Azign Bioscience As | Procedimiento para clonar arnm y visualizar transcriptores diferencialmente expresados (dodets). |
EP0921196A1 (fr) * | 1997-12-02 | 1999-06-09 | Roche Diagnostics GmbH | Polymérase d'ADN modifiée de Carboxydothermus hydrogenoformans et son utilisation pour transcription inverse suivi par la réaction en chaíne de la polymérase |
EP0922765B1 (fr) * | 1997-12-02 | 2005-11-23 | Roche Diagnostics GmbH | Polymérase d'ADN modifiée de Carboxydothermus hydrogenoformans et son utilisation pour transcription inverse suivi par la réaction en chaíne de la polymérase |
WO1999047539A1 (fr) * | 1998-03-18 | 1999-09-23 | Amersham Pharmacia Biotech, Inc. | Polymerase d'adn thermostable provenant du thermoanaerobacter thermohydrosulfuricus |
AT410218B (de) | 1999-08-20 | 2003-03-25 | Baxter Ag | Verfahren zur herstellung eines qualitätsgesicherten pools biologischer proben |
US6436677B1 (en) | 2000-03-02 | 2002-08-20 | Promega Corporation | Method of reverse transcription |
US6632645B1 (en) | 2000-03-02 | 2003-10-14 | Promega Corporation | Thermophilic DNA polymerases from Thermoactinomyces vulgaris |
KR20000072141A (ko) * | 2000-08-04 | 2000-12-05 | 김유삼 | 신규한 호열성 단백질 분해효소의 dna 서열 및그로부터 유래된 단백질 |
GB0205455D0 (en) | 2002-03-07 | 2002-04-24 | Molecular Sensing Plc | Nucleic acid probes, their synthesis and use |
US7575864B2 (en) * | 2004-05-27 | 2009-08-18 | E.I. Du Pont De Nemours And Company | Method for the direct detection of diagnostic RNA |
US7504240B2 (en) * | 2005-03-10 | 2009-03-17 | Masanori Hirano | Methods for synthesizing polynucleotides using a single primer |
JP5191041B2 (ja) | 2007-04-05 | 2013-04-24 | エフ.ホフマン−ラ ロシュ アーゲー | 急速ワンステップrt−pcr |
KR101039521B1 (ko) * | 2008-02-01 | 2011-06-08 | 주식회사 인트론바이오테크놀로지 | 금속 이온에 의해 발생하는 pcr 저해 및 비특이 증폭을완화시키거나 막을 수 있는 에틸렌글리콜테트라아세틱 산을킬레이트제로 포함하는 pcr 반응용 조성물 |
US9045729B2 (en) | 2009-12-10 | 2015-06-02 | Ottawa Hospital Research Institute | Oncolytic rhabdovirus |
US9364532B2 (en) | 2011-06-08 | 2016-06-14 | Children's Hospital Of Eastern Ontario Research Institute Inc. | Compositions and methods for glioblastoma treatment |
WO2013185081A1 (fr) * | 2012-06-08 | 2013-12-12 | Ionian Technologies, Inc | Amplifications d'acide nucléique |
EP3622965A1 (fr) | 2013-02-21 | 2020-03-18 | Turnstone Limited Partnership | Composition de vaccin |
EP3099810B1 (fr) | 2014-01-31 | 2019-05-01 | Qiagen GmbH | Démarrage à chaud pour chélateur cationique |
US10301660B2 (en) | 2015-03-30 | 2019-05-28 | Takara Bio Usa, Inc. | Methods and compositions for repair of DNA ends by multiple enzymatic activities |
US11236384B2 (en) | 2017-01-19 | 2022-02-01 | Asuragen, Inc. | Methods of RNA amplification |
GB201812192D0 (en) | 2018-07-26 | 2018-09-12 | Ttp Plc | Variable temperature reactor, heater and control circuit for the same |
CN110846724B (zh) * | 2019-12-03 | 2023-04-28 | 江西海普洛斯医学检验实验室有限公司 | 构建mRNA链特异文库的方法及试剂盒 |
CN113045410A (zh) * | 2021-03-29 | 2021-06-29 | 华东理工大学 | 一种双环降二萜类化合物及其合成基因及制备方法 |
EP4074839A1 (fr) | 2021-04-16 | 2022-10-19 | Biotype GmbH | Sonde tx oligonucléotidique optimisée pour une analyse de multiplexage d'acides nucléiques et méthode de multiplexage |
EP4350002A1 (fr) | 2022-10-07 | 2024-04-10 | Biotype GmbH | Detection of molecular analytes based on tailored probe competition |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA1338457C (fr) * | 1986-08-22 | 1996-07-16 | Henry A. Erlich | Enzyme thermostable purifiee |
-
1990
- 1990-12-21 AT AT91904087T patent/ATE151112T1/de not_active IP Right Cessation
- 1990-12-21 WO PCT/US1990/007641 patent/WO1991009944A2/fr active IP Right Grant
- 1990-12-21 ES ES91904087T patent/ES2100945T3/es not_active Expired - Lifetime
- 1990-12-21 DE DE69030386T patent/DE69030386T2/de not_active Expired - Lifetime
- 1990-12-21 DK DK91904087.3T patent/DK0506889T3/da active
- 1990-12-21 EP EP91904087A patent/EP0506889B1/fr not_active Expired - Lifetime
- 1990-12-21 AU AU72444/91A patent/AU656315B2/en not_active Expired
- 1990-12-21 CA CA002071213A patent/CA2071213C/fr not_active Expired - Lifetime
- 1990-12-21 JP JP3504344A patent/JP2968585B2/ja not_active Expired - Lifetime
-
1997
- 1997-06-24 GR GR970401505T patent/GR3023862T3/el unknown
Also Published As
Publication number | Publication date |
---|---|
GR3023862T3 (en) | 1997-09-30 |
DE69030386D1 (de) | 1997-05-07 |
EP0506889B1 (fr) | 1997-04-02 |
WO1991009944A3 (fr) | 1991-08-22 |
JP2968585B2 (ja) | 1999-10-25 |
CA2071213C (fr) | 2000-05-02 |
DE69030386T2 (de) | 1997-10-09 |
ATE151112T1 (de) | 1997-04-15 |
CA2071213A1 (fr) | 1991-06-23 |
EP0506889A1 (fr) | 1992-10-07 |
AU7244491A (en) | 1991-07-24 |
JPH05505105A (ja) | 1993-08-05 |
ES2100945T3 (es) | 1997-07-01 |
WO1991009944A2 (fr) | 1991-07-11 |
AU656315B2 (en) | 1995-02-02 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DK0506889T3 (da) | Reverse transkriptaser ved høj temperatur | |
US6399320B1 (en) | Modified DNA-polymerase from carboxydothermus hydrogenoformans and its use for coupled reverse transcription and polymerase chain reaction | |
EP0929680B1 (fr) | Polymerase d'adn thermostable provenant de carboxydothermus hydrogenoformans | |
CN103987843B (zh) | 具有改进活性的dna聚合酶 | |
NO931709L (no) | Nukleinsyreamplifisering ved hjelp av to-enzymers, selvunderholdende sekvensreplikasjon | |
EP2272952B8 (fr) | ADN polymérase thermostable ou thermoactif ayant une activité 3' - 5' exonucléase atténuée | |
EP0428197A3 (fr) | Méthodes pour l'extraction des acides nucléiques et pour les amplifier par PCR sans utiliser un enzyme protéolytique | |
CN103987844B (zh) | 具有改进活性的dna聚合酶 | |
DE69426400D1 (de) | Thermostable Nukleinsäure Polymerase | |
AU3062989A (en) | Purified thermostable enzyme | |
DK0669401T3 (da) | Amplificering af lange nukleinsyresekvenser ved PCR | |
JPH05506364A (ja) | 熱安定性 dnaポリメラーゼの5→3′のエキソヌクレアーゼ突然変異 | |
DK1705253T3 (da) | Sammensætninger og fremgangsmåder til revers transkriptase-polymerasekædereaktion (RT-PCR) | |
CN103687943B (zh) | 具有改进活性的dna聚合酶 | |
CA2127188A1 (fr) | Reactifs et methodes pour coupler la transcription inverse a haute temperature et les reactions d'amplification par la polymerase | |
EP0272098A3 (fr) | Procédé d'amplification et de détection de séquences ARN | |
DE69737024D1 (en) | Thermostabiles nukleinsäure-polymerase von thermococcus gorgonarius | |
US6692932B1 (en) | Thermostable DNA polymerase from anaerocellum thermophilum | |
RU2221867C2 (ru) | Очищенная термостабильная днк-полимераза из thermococcus gorgonarius, ее получение и применение | |
Gelfand et al. | Reverse transcription with thermus thermophilus polymerase | |
ATE367452T1 (de) | Methode zur reversen transkription von im getrockneten zustand isolierter rna |