DK0408542T3 - Method for determining antigens and/or antibodies in human body fluids and test kit for carrying out the method - Google Patents

Method for determining antigens and/or antibodies in human body fluids and test kit for carrying out the method

Info

Publication number
DK0408542T3
DK0408542T3 DK90890206.7T DK90890206T DK0408542T3 DK 0408542 T3 DK0408542 T3 DK 0408542T3 DK 90890206 T DK90890206 T DK 90890206T DK 0408542 T3 DK0408542 T3 DK 0408542T3
Authority
DK
Denmark
Prior art keywords
antibodies
antigens
agb1
aga1
human body
Prior art date
Application number
DK90890206.7T
Other languages
Danish (da)
Inventor
Ewald Dr Molinari
Helmut Dr Aicher
Original Assignee
Immuno Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Immuno Ag filed Critical Immuno Ag
Application granted granted Critical
Publication of DK0408542T3 publication Critical patent/DK0408542T3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302—Apparatus specially adapted for immunological test procedures
    • G01N33/5304—Reaction vessels, e.g. agglutination plates

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

For the simultaneous determination of a plurality of antigens and/or antibodies in a sample of a human body fluid, the sample is introduced into a microtitre plate whose wells are coated with antibodies (Aba1, Aba2 ...) which are directed against some of the antigens which are sought (Aga1, Aga2, ...), and a plurality of solid carriers, preferably in the form of a comb, which are coated with antibodies (Abb1, Abb2...) which are directed against others of the antigens which are sought (Agb1, Agb2...) are contacted with the sample; then, after complexation has taken place, the wells and the solid carriers are washed free of non-adsorbed proteins, the complexes (Aba1-Aga1 ... and/or Abb1-Agb1 ...) which are adsorbed in the wells of the microtitre plate and/or to the solid carriers are incubated with a mixture of labelled antibody enzymes (Aba1E<*> ... Abb1E<*>, ...) which are directed against the antigens which are sought (Aga1, Aga2, ... Agb1, Agb2...), and the activity of the enzymes (Aba1-Aga1-Aba1E<*>, ... Abb1-Agb1-Abb1E<*>, ...) bound to the complexes is determined by photometry after reaction with a colour-forming substrate.
DK90890206.7T 1989-07-13 1990-07-09 Method for determining antigens and/or antibodies in human body fluids and test kit for carrying out the method DK0408542T3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
AT0169989A AT394114B (en) 1989-07-13 1989-07-13 METHOD FOR DETERMINING ANTIGENS AND / OR ANTIBODIES IN HUMAN BODY LIQUIDS, AND SET FOR CARRYING OUT THE METHOD

Publications (1)

Publication Number Publication Date
DK0408542T3 true DK0408542T3 (en) 1996-01-22

Family

ID=3519074

Family Applications (1)

Application Number Title Priority Date Filing Date
DK90890206.7T DK0408542T3 (en) 1989-07-13 1990-07-09 Method for determining antigens and/or antibodies in human body fluids and test kit for carrying out the method

Country Status (9)

Country Link
EP (1) EP0408542B1 (en)
JP (1) JPH0353166A (en)
AT (2) AT394114B (en)
CA (1) CA2021048A1 (en)
DE (1) DE59009676D1 (en)
DK (1) DK0408542T3 (en)
ES (1) ES2081363T3 (en)
FI (1) FI903504A0 (en)
NO (1) NO903107L (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2228821A1 (en) * 1998-04-16 1999-10-16 Zhibo Gan Pin method for measuring bioactive substance
EP2631007A1 (en) 2012-02-22 2013-08-28 Roche Diagniostics GmbH Device for parallelization and performance increase in microarray-immunoassays with solid, non-porous capture-zone

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT343822B (en) * 1976-08-20 1978-06-26 Immuno Ag RADIOIMMUNOLOGICAL METHOD AND EQUIPMENT FOR DETERMINING ANTIGENES
CA1148859A (en) * 1979-06-14 1983-06-28 Lacy R. Overby Simultaneous assay of two hepatitis viruses using a solid phase
GB2107053A (en) * 1980-06-20 1983-04-20 Unilever Plc Processes and apparatus for carrying out specific binding assays
GB2084317B (en) * 1980-09-30 1984-01-18 Erba Farmitalia Antigen-linked competitive enzymeimmunoassay
EP0106855A1 (en) * 1982-04-16 1984-05-02 Genefusion S.A. Analysis device for biological symbols

Also Published As

Publication number Publication date
DE59009676D1 (en) 1995-10-26
JPH0353166A (en) 1991-03-07
ATE128232T1 (en) 1995-10-15
NO903107L (en) 1991-01-14
EP0408542A2 (en) 1991-01-16
AT394114B (en) 1992-02-10
NO903107D0 (en) 1990-07-12
EP0408542B1 (en) 1995-09-20
FI903504A0 (en) 1990-07-11
CA2021048A1 (en) 1991-01-14
EP0408542A3 (en) 1993-01-13
ATA169989A (en) 1991-07-15
ES2081363T3 (en) 1996-03-01

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Legal Events

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