DE975799T1 - METHOD FOR IDENTIFYING NUCLEIC ACID SEQUENCES RELATING TO APOMIXIA - Google Patents

METHOD FOR IDENTIFYING NUCLEIC ACID SEQUENCES RELATING TO APOMIXIA

Info

Publication number
DE975799T1
DE975799T1 DE0975799T DE98909558T DE975799T1 DE 975799 T1 DE975799 T1 DE 975799T1 DE 0975799 T DE0975799 T DE 0975799T DE 98909558 T DE98909558 T DE 98909558T DE 975799 T1 DE975799 T1 DE 975799T1
Authority
DE
Germany
Prior art keywords
apomictic
sequence
genome
maize
development
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
DE0975799T
Other languages
German (de)
Inventor
Daniel Grimanelli
Olivier Leblanc
Enrico Perotti
Yves Savidan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut de Recherche pour le Developpement IRD
CIMMYT ABC MEXICO
Original Assignee
Institut de Recherche pour le Developpement IRD
CIMMYT ABC MEXICO
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut de Recherche pour le Developpement IRD, CIMMYT ABC MEXICO filed Critical Institut de Recherche pour le Developpement IRD
Publication of DE975799T1 publication Critical patent/DE975799T1/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Claims (24)

PatentansprüchePatent claims 1. Verfahren zum Identifizieren einer Nucleotidsequenz, die ortholog zu der für die vollständige oder teilweise apomiktische Entwicklung bei einer apomiktischen Form verantwortlichen Sequenz ist, bei einem Gras und im spezielleren bei einem Mais, dadurch gekennzeichnet, daß man in dem Genom des Grases, im spezielleren eines Maises, Mutationen kartographiert, die einen verwandten oder ähnlichen Phänotyp zu dem bei einer apomiktischen Form festgestellten Phänotyp aufweisen, um jene zu identifizieren, die zu Genen ortholog erscheinen, die in die Apomixie involviert sind.1. A method for identifying a nucleotide sequence orthologous to the sequence responsible for total or partial apomictic development in an apomictic form in a grass, and more particularly in maize, characterized in that mutations are mapped in the genome of the grass, more particularly of a maize, which have a related or similar phenotype to the phenotype observed in an apomictic form in order to identify those which appear orthologous to genes involved in apomictic development. 2. Verfahren nach Anspruch 1, dadurch gekennzeichnet, daß man im Genom des Grases, im spezielleren eines Maises, meiotische Mutationen kartographiert, um jene zu identifizieren, die ortholog zu in der Apomixie involvierten Genen erscheinen.2. Process according to claim 1, characterized in that meiotic mutations are mapped in the genome of the grass, more particularly of maize, in order to identify those which appear orthologous to genes involved in apomixia. 3. Verfahren nach Anspruch 2, dadurch gekennzeichnet, daß man die Position der verschiedenen meiotischen Mutationen im Genom des Grases, im spezielleren eines Maises, mit Hilfe von molekularen Markern lokalisiert, die zum Lokalisieren der für die Apomeiose verantwortlichen Loci bei der genannten apomiktischen Form befähigt sind.3. Process according to claim 2, characterized in that the position of the various meiotic mutations in the genome of the grass, more particularly of maize, is localized using molecular markers capable of localizing the loci responsible for apomeiosis in said apomictic form. 4. Verfahren nach Anspruch 3, dadurch gekennzeichnet, daß man molekulare Marker verwendet, die -zum Lokalisieren der für die Diplosporie bei Tripsacum verantwortlichen Loci befähigt sind.4. Process according to claim 3, characterized in that molecular markers are used which are capable of locating the loci responsible for diplosporia in Tripsacum . 5. Verfahren nach Anspruch 4, dadurch gekennzeichnet, daß die genannte Lokalisierung die Loci elongate und afd betrifft.5. Method according to claim 4, characterized in that said localization concerns the loci elongate and afd. 6. Verfahren nach einem der Ansprüche 1 bis 5, dadurch gekennzeichnet, daß es weiterhin die Markierung durch6. A method according to any one of claims 1 to 5, characterized in that it further comprises marking by Transposition der lokalisierten meiotischen Mutationen umfaßt.Transposition of localized meiotic mutations. 7. Verfahren nach Anspruch 6, dadurch gekennzeichnet, daß die Markierung durch Transposition am Locus elongate mit Hilfe von transposablen Elementen vom Typ Mutator oder Ac/Ds realisiert wird.7. Method according to claim 6, characterized in that the marking is carried out by transposition at the locus elongate using transposable elements of the Mutator or Ac/Ds type. 8. Verfahren nach einem der vorstehenden Ansprüche, gekennzeichnet durch die Klonierung und Sequenzierung der lokalisierten Mutationen.8. Method according to one of the preceding claims, characterized by cloning and sequencing the localized mutations. 9. Verfahren nach Anspruch 6 oder 7, dadurch gekennzeichnet, daß man die mutierten Gene kloniert, nachdem man die Insertionsstelle des Transposons durch Segregationsanalyse geortet hat, und daß man sie gewünschtenfalls sequenziert.9. Method according to claim 6 or 7, characterized in that the mutated genes are cloned after the insertion site of the transposon has been located by segregation analysis and that they are sequenced if desired. 10. Nucleotidsequenzen, dadurch gekennzeichnet, daß sie zu Sequenzen ortholog sind, die zur Gänze oder teilweise für die apomiktische Entwicklung bei einer apomiktischen Form verantwortlich sind, und die homologen Sequenzen.10. Nucleotide sequences, characterized in that they are orthologous to sequences which are wholly or partly responsible for the apomictic development in an apomictic form, and the homologous sequences. 11. Nucleotidsequenz nach Anspruch 10, dadurch gekennzeichnet, daß sie dem elongat-mutierten Gen entspricht.11. Nucleotide sequence according to claim 10, characterized in that it corresponds to the elongate mutated gene. 12. Nucleinsäuren mit einem Gehalt an einer oder an mehreren Sequenzen, wie im Anspruch 10 oder 11 definiert, gebunden an Regulatorsequenzen, die zur Expression in einem Pflanzenmaterial erforderlich sind.12. Nucleic acids containing one or more sequences as defined in claim 10 or 11 linked to regulatory sequences required for expression in a plant material. Klonierungs- und Expressionsvektoren, ""■ umfassend die Nucleinsäuren nach Anspruch 12.Cloning and expression vectors ""■ comprising the nucleic acids according to claim 12. 14. Zelluläre Wirte mit einem Gehalt an einem Vektor nach Anspruch 13.14. Cellular hosts containing a vector according to claim 13. 15. Verwendung einer Sequenz nach Anspruch 10 oder 11, gegebenenfalls in Verbindung mit anderen Allelen, die für apomiktische Formen charakteristisch sind, zur Einführung15. Use of a sequence according to claim 10 or 11, optionally in combination with other alleles characteristic of apomictic forms, for introducing U:-L-3U:t· W-hp O 375739 T1 U : -L-3U : t W-hp O 375739 T1 in das Genom eines Pflanzenmaterials, in Pflanzenzellen,into the genome of a plant material, into plant cells, Pflanzen in verschiedenen Entwicklungsstadien und inPlants in different stages of development and in Samen, um in ihnen eine apomiktische Entwicklung hervorzurufen. seeds to induce apomictic development in them. 16. Pflanzenzelle von Gräsern, insbesondere von Mais, dadurch gekennzeichnet, daß sie in ihrem Genom wenigstens einen Teil einer Sequenz gemäß Anspruch 10 oder 11 trägt, die in einer apomiktischen Entwicklung impliziert ist.16. Plant cell of grasses, in particular of maize, characterized in that it carries in its genome at least part of a sequence according to claim 10 or 11, which is implicated in an apomictic development. 17. Pflanze aus der Familie der Gräser, insbesondere Mais, dadurch gekennzeichnet, daß sie in ihrem Genom wenigstens einen Teil einer Sequenz nach Anspruch 10 oder 11 enthält, die in einer apomiktischen Entwicklung impliziert ist.17. Plant from the grass family, in particular maize, characterized in that it contains in its genome at least a part of a sequence according to claim 10 or 11 which is implicated in an apomictic development. 18. Gräsersamen, insbesondere von Mais, dadurch gekennzeichnet, daß er in seinem Genom wenigstens einen Teil einer Sequenz nach Anspruch 10 oder 11 enthält, die in einer apomiktischen Entwicklung impliziert ist.18. Grass seed, in particular of maize, characterized in that it contains in its genome at least a part of a sequence according to claim 10 or 11, which is implicated in an apomictic development. 19. Verfahren zur Herstellung von apomiktischen Pflanzen, dadurch gekennzeichnet, daß man eine Nucleotidsequenz nach Anspruch 11 anwendet.19. A process for the production of apomictic plants, characterized in that a nucleotide sequence according to claim 11 is used. 20. Verwendung wenigstens eines Teils einer Sequenz nach Anspruch 10 oder 11 zum Identifizieren und Isolieren von Locussequenzen, die ortholog bei apomiktischen Formen sind.20. Use of at least part of a sequence according to claim 10 or 11 for identifying and isolating locus sequences which are orthologous in apomictic forms. 21. Hybridisationssonden und Molekularprimer, dadurch gekennzeichnet, daß sie ausgehend von einer Sequenz nach Anspruch 10 oder 11 gebildet sind.21. Hybridization probes and molecular primers, characterized in that they are formed from a sequence according to claim 10 or 11. 22. Hybridisationssonden und Molekularprimer nach Anspruch 18, dadurch gekennzeichnet, daß sie von der Sequenz elongate ausgehend gebildet sind.22. Hybridization probes and molecular primers according to claim 18, characterized in that they are formed starting from the sequence elongate. 23. Verfahren zum Identifizieren und Isolieren von Genen, die für die Apomeiose bei apomiktischem Tripsacum verant-23. Method for identifying and isolating genes responsible for apomeiosis in apomictic Tripsacum * &iacgr;* &iacgr; wortlich sind, dadurch gekennzeichnet, daß man wenigstens einen Teil der Sequenz des Locus elongate verwendet.literal, characterized in that at least part of the sequence of the locus elongate is used. 24. Verfahren zur Anwendung einer Mutagenesepopulation zur Bestätigung der Relation zwischen einer bei Tripsacum gemäß Anspruch 20 isolierten Sequenz und der Expression von Apomixie.24. A method of using a mutagenesis population to confirm the relationship between a sequence isolated in Tripsacum according to claim 20 and the expression of apomixia.
DE0975799T 1997-02-17 1998-02-17 METHOD FOR IDENTIFYING NUCLEIC ACID SEQUENCES RELATING TO APOMIXIA Pending DE975799T1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR9701821A FR2759708B1 (en) 1997-02-17 1997-02-17 MEANS FOR IDENTIFYING, ISOLATING AND CHARACTERIZING NUCLEOTIDE SEQUENCES INVOLVED IN APOMIXIA
PCT/FR1998/000308 WO1998036090A1 (en) 1997-02-17 1998-02-17 Means for identifying nucleotide sequences involved in apomixis

Publications (1)

Publication Number Publication Date
DE975799T1 true DE975799T1 (en) 2000-08-31

Family

ID=9503800

Family Applications (1)

Application Number Title Priority Date Filing Date
DE0975799T Pending DE975799T1 (en) 1997-02-17 1998-02-17 METHOD FOR IDENTIFYING NUCLEIC ACID SEQUENCES RELATING TO APOMIXIA

Country Status (8)

Country Link
EP (1) EP0975799A1 (en)
CN (1) CN1248296A (en)
AU (1) AU6405498A (en)
CA (1) CA2282085A1 (en)
DE (1) DE975799T1 (en)
ES (1) ES2146561T1 (en)
FR (1) FR2759708B1 (en)
WO (1) WO1998036090A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR0015276A (en) * 1999-10-29 2002-07-16 Univ Utah State Methods to stabilize and control apomixis
US7264964B2 (en) 2001-06-22 2007-09-04 Ceres, Inc. Chimeric histone acetyltransferase polypeptides
US7476777B2 (en) 2002-09-17 2009-01-13 Ceres, Inc. Biological containment system
US8878002B2 (en) 2005-12-09 2014-11-04 Council Of Scientific And Industrial Research Nucleic acids and methods for producing seeds with a full diploid complement of the maternal genome in the embryo
FR2952276A1 (en) * 2009-11-09 2011-05-13 Inst Rech Developpement Ird INDUCTION OF APOMIXY IN CULTIVATED PLANTS WITH SEXUAL REPRODUCTION AND USE FOR REPRODUCTION OF TOTALLY OR PARTIALLY APOMICALLY ACTIVE PLANTS
EP2530160A1 (en) 2011-05-30 2012-12-05 Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung Gatersleben (IPK) Means and methods to induce apomixis in plants
WO2017039452A1 (en) * 2015-09-04 2017-03-09 Keygene N.V. Diplospory gene

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5811636A (en) * 1995-09-22 1998-09-22 The United States Of America As Represented By The Secretary Of Agriculture Apomixis for producing true-breeding plant progenies
US5710367A (en) * 1995-09-22 1998-01-20 The United States Of America As Represented By The Secretary Of Agriculture Apomictic maize

Also Published As

Publication number Publication date
EP0975799A1 (en) 2000-02-02
AU6405498A (en) 1998-09-08
FR2759708A1 (en) 1998-08-21
CN1248296A (en) 2000-03-22
ES2146561T1 (en) 2000-08-16
FR2759708B1 (en) 1999-08-27
WO1998036090A1 (en) 1998-08-20
CA2282085A1 (en) 1998-08-20

Similar Documents

Publication Publication Date Title
Fyrberg et al. The actin genes of Drosophila: protein coding regions are highly conserved but intron positions are not
Stoker et al. A highly polymorphic region 3′ to the human type II collagen gene
Pedersen et al. Cloning and sequence analysis reveal structural variation among related zein genes in maize
Kim et al. Sequence and expression studies of A-, B-, and E-class MADS-box homologues in Eupomatia (Eupomatiaceae): support for the bracteate origin of the calyptra
Avramova et al. Matrix attachment regions and transcribed sequences within a long chromosomal continuum containing maize Adh1.
DE19960843A1 (en) Root-specific promoter
CN110592251B (en) Main effect QTL locus of flowering phase character of brassica napus, SNP molecular marker development and application
Manchado et al. Molecular characterization and chromosomal mapping of the 5S rRNA gene in Solea senegalensis: a new linkage to the U1, U2, and U5 small nuclear RNA genes
CN110468218A (en) A kind of detection method of goat IGF2BP1 gene insertion/deletion label
CN101967480A (en) Molecular marker relevant to chicken skin color and authentication method and application thereof
DE975799T1 (en) METHOD FOR IDENTIFYING NUCLEIC ACID SEQUENCES RELATING TO APOMIXIA
Cheng Mapping the chicken genome
CN107164468A (en) Polymorphic micro-satellite DNA molecular marker for deer and application thereof
Hoppe et al. Promoter motifs of olfactory receptor genes expressed in distinct topographic patterns
DE19525284A1 (en) Microsatellite markers for plants of the species Triticum aestivum and of the Tribus Triticeae and their use
CN106498048B (en) One kind QTL relevant to soybean nodulation number, SNP marker and application
CN110093346A (en) With the molecular marker and application thereof of purple tsai-tai a kind of sedge color gene linkage
DE03779067T1 (en) FLUORESCENCE PROTEINS AND CHROMOPROTEINS FROM NON-AEQUOREA HYDROZOA SPECIES AND METHOD FOR USE THEREOF
KR20160053420A (en) SNP molecular markers associated with the pungency of chili and uses thereof
Gong et al. Development and characterization of 13 polymorphic microsatellite DNA markers for the pond green frog (Rana nigromaculata)
EP3380618B1 (en) Plant with tolerance of the cold
Soto-Calderón et al. Factors affecting the relative abundance of nuclear copies of mitochondrial DNA (numts) in hominoids
Tuggle et al. Cloning and characterization of pig muscle cDNAs by an expressed sequence tag approach
JP4581101B2 (en) Papaya sex identification method using DNA marker
KR101961855B1 (en) PHD gene involved in pollen development in plants and method for discriminating genic male sterility of plant by using the gene