DE847470C - Device for the numerical recording of the amount of suspended particles in a solution - Google Patents

Device for the numerical recording of the amount of suspended particles in a solution

Info

Publication number
DE847470C
DE847470C DEL833D DEL0000833D DE847470C DE 847470 C DE847470 C DE 847470C DE L833 D DEL833 D DE L833D DE L0000833 D DEL0000833 D DE L0000833D DE 847470 C DE847470 C DE 847470C
Authority
DE
Germany
Prior art keywords
solution
photocell
amount
suspended particles
chamber
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DEL833D
Other languages
German (de)
Inventor
Max Dr Berek
Werner Kalensee
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ernst Leitz Wetzlar GmbH
Original Assignee
Ernst Leitz Wetzlar GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ernst Leitz Wetzlar GmbH filed Critical Ernst Leitz Wetzlar GmbH
Priority to DEL833D priority Critical patent/DE847470C/en
Application granted granted Critical
Publication of DE847470C publication Critical patent/DE847470C/en
Expired legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/47Scattering, i.e. diffuse reflection
    • G01N21/49Scattering, i.e. diffuse reflection within a body or fluid
    • G01N21/51Scattering, i.e. diffuse reflection within a body or fluid inside a container, e.g. in an ampoule

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Description

Es ist bekannt, Blutkörperchen in Zählkammern @uszuzählen. L)ieses Verfahren ist zeitraubend uncl vor allem bei Reihenuntersuchungen sehr ermüdend, so daß leicht Fehler entstehen können. It is known to count blood cells in counting chambers. Read it The procedure is time-consuming and, especially in the case of serial examinations, very tiring, so that mistakes can easily arise.

Man hat nun bereits zum Zweck der Trübungsmessung durch die Küvette hindurch eine Lichtquelle abgebildet und das durch die suspendierten Teilchen gestreute Licht im Verhältnis zum ungestreuten Licht rnit Photozellen gemessen und so das Maß der Trübung erfaßt, Hierzu sind aber größere Mengen der zu untersuchenden Flüssigkeit nötig. You now have to measure the turbidity through the cuvette imaged through a light source and that scattered by the suspended particles Light in relation to unscattered light measured with photocells and so that The degree of turbidity recorded, but larger amounts of the liquid to be examined are required for this necessary.

Der vorliegenden Erfindung liegt nun die Aufgabe zugrunde, eine Einrichtung zu schaffen mit der die Zählung suspendierter Teilchen an Hand kleinster Mengen mit großer Genauigkeit durchgeführt werden kann. Zu diesem Zweck wird die die Teilchen enthaltende Lösung in einer Küvette in die Strahlenführung einer Dunkelfeldbeleuchtung eingeschaltet. Die Teilchen wirken dann als Selbstleuchter, deren Licht eine über der Kammer angeordllebte Photozelle je nach der Zahl der @eilchen mehr oder weniger erregt. Der Ausschlag eines Galvanometerzeigers über einer entsprechend geeichten Skala gibt dann sofort die Zahl der Teilchen mit ausreichender Genauigkeit an. Eine besonders zweckmäßige Anordnung ergibt sich dadurch, daß man das zu beleuchtende Strahlenbündel aufspaltet und zum Teil direkt einer Photozelle zuführt und zum anderen Teil für die Dunkelfeldbeleuchtung verwendet. Die Photozelle, die die vom Objekt kommenden Strahlen aufnimmt, ist dann mit der direkt betroffenen Zelle in einer Kompensationsschaltung zusammengefaßt. Wenn man außerdem die direkt auf die eine Zelle fallenden Strahlen in dem Maße schwächt, wie diese z. B. durch eine normale Blutzusammensetzung erfolgen würde, so kann man an der Abweichung des Galvanometerzeigers vom Nullpunkt die Unregelmäßigkeiten mengenmäßig sofort bestimmen. The present invention is based on the object of a device to create with the counting of suspended particles on the basis of the smallest quantities can be carried out with great accuracy. To this end, the the particles containing solution in a cuvette into the beam guide of a dark field illumination switched on. The particles then act as self-luminosity, whose light one over The photocell placed in the chamber is more or less depending on the number of particles excited. The deflection of a galvanometer pointer over a suitably calibrated The scale then immediately indicates the number of particles with sufficient accuracy. One a particularly expedient arrangement results from the fact that one is to be illuminated Beams split up and partly fed directly to a photocell and partly directly to a photocell Part used for dark field lighting. The photocell used by the object absorbs incoming rays is then in one with the directly affected cell Compensation circuit summarized. If you also click the one directly Cell falling rays weakens to the extent that this z. B. through a normal Blood composition would be done, one can look at the deviation of the galvanometer pointer Determine the quantity of the irregularities immediately from the zero point.

In der Zeichnung ist die Erfindung an Hand einer Blutkörperzähleinrichtung beispielsweise dargestellt. In the drawing, the invention is shown on the basis of a blood cell counting device for example shown.

Das von der Lichtquelle 1 ausgehende und von dem Kondensor 2 gerichtete Strahlenbündel fällt mit seinem zentralen Teil nach Durchdringen des Filters 3 auf die Photozelle 4. Der äußere Teil des Strahlenbündels wird von dem Ringspiegel 5 reflektiert und dann von dem zentral abgeblendeten Kondensor 6 (Dunkelfeldkondensor) zur Beleuchtung der Zählkammer 7 gesammelt. Die durch die Kammer hindurchfallenden Strahlen werden von dem durch die Blende 9 abgegrenzten Dunkelraum ab sorbiert, während auf die Photozelle 8 lediglich die von den suspendierten Teilchen ausgehenden Strahlett fallen. That emanating from the light source 1 and directed by the condenser 2 The central part of the bundle of rays is noticeable after passing through the filter 3 the photocell 4. The outer part of the beam is from the ring mirror 5 reflected and then by the centrally shielded condenser 6 (dark field condenser) collected to illuminate the counting chamber 7. The ones falling through the chamber Rays are absorbed from the dark space delimited by the diaphragm 9, while on the photocell 8 only those emanating from the suspended particles Strahlett fall.

Wählt man das Filter 3 so, laß siclt tlie von gleit Photozellen 4 und 5 erzeugten Ströme dann kompensieren, wenn sich in der Kammer 7 normales Blut befindet, so wird jedes anders zusammengesetzte Blut durch den Ausschlag eines nicht dar gestellten Galvanometerzeigers angegeben. If you choose the filter 3 like this, let the photocells 4 slide and 5 then compensate for currents generated when there is normal blood in the chamber 7 every differently composed blood is not caused by the rash of one shown galvanometer pointer indicated.

Claims (2)

P A T E N T A N S P R Ü C H E : 1. Einrichtung zum zahlenmäßigen Erfassen der Menge in einer Lösung suspendierter Teilchen unter Verwendung von Photozellen und einem elektrischen Anzeigegerät, dadurch gekennzeichnet, daß die Kammer mit der Lösung in den Strahlengang einer Dunkelfeldbeleuch tung eingeschaltet wird und daß die Photozelle so über der Kammer angeordnet ist, daß sie von den unmittelbaren Beleuchtungsstrahlen nicht getroffen wird. P A T E N T A N S P R Ü C H E: 1. Facility for numerical Detection of the amount of particles suspended in a solution using photocells and an electrical display device, characterized in that the chamber with the solution is switched into the beam path of a dark field lighting device and that the photocell is arranged above the chamber so that it is of the immediate Illumination rays is not hit. 2. Einrichtung nacli Anspruch 1 , dadurch gekennzeichnet, daß ein Teil der Beleuchtungsstrahlen zur Dunkelfeldbeleuchtung dient und der andere Teil direkt auf eine zweite Photozelle fällt, die mit der ersten Photozelle in Kompensation geschaltet ist. 2. Device according to claim 1, characterized in that a Part of the lighting beams is used for dark field lighting and the other part falls directly on a second photocell, which is in compensation with the first photocell is switched.
DEL833D 1943-12-19 1943-12-19 Device for the numerical recording of the amount of suspended particles in a solution Expired DE847470C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DEL833D DE847470C (en) 1943-12-19 1943-12-19 Device for the numerical recording of the amount of suspended particles in a solution

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DEL833D DE847470C (en) 1943-12-19 1943-12-19 Device for the numerical recording of the amount of suspended particles in a solution

Publications (1)

Publication Number Publication Date
DE847470C true DE847470C (en) 1952-08-25

Family

ID=7255098

Family Applications (1)

Application Number Title Priority Date Filing Date
DEL833D Expired DE847470C (en) 1943-12-19 1943-12-19 Device for the numerical recording of the amount of suspended particles in a solution

Country Status (1)

Country Link
DE (1) DE847470C (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3341749A1 (en) * 1982-11-19 1984-05-24 Shimadzu Corp., Kyoto BLOOD METER

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3341749A1 (en) * 1982-11-19 1984-05-24 Shimadzu Corp., Kyoto BLOOD METER

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