DE69941655D1 - Elektrophoretischer Trennung von Nukleinsäuren aus biologischen Proben bei niedrigem pH-Wert - Google Patents

Elektrophoretischer Trennung von Nukleinsäuren aus biologischen Proben bei niedrigem pH-Wert

Info

Publication number
DE69941655D1
DE69941655D1 DE69941655T DE69941655T DE69941655D1 DE 69941655 D1 DE69941655 D1 DE 69941655D1 DE 69941655 T DE69941655 T DE 69941655T DE 69941655 T DE69941655 T DE 69941655T DE 69941655 D1 DE69941655 D1 DE 69941655D1
Authority
DE
Germany
Prior art keywords
nucleic acids
low
sample
biological samples
electrophoretic separation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69941655T
Other languages
English (en)
Inventor
Carol Ann Kreader
John Wesley Backus
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ortho Clinical Diagnostics Inc
Original Assignee
Ortho Clinical Diagnostics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ortho Clinical Diagnostics Inc filed Critical Ortho Clinical Diagnostics Inc
Application granted granted Critical
Publication of DE69941655D1 publication Critical patent/DE69941655D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Electrostatic Separation (AREA)
DE69941655T 1998-08-04 1999-08-03 Elektrophoretischer Trennung von Nukleinsäuren aus biologischen Proben bei niedrigem pH-Wert Expired - Lifetime DE69941655D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9525898P 1998-08-04 1998-08-04
US09/349,387 US6699986B2 (en) 1998-08-04 1999-07-09 Electrophoretic separation of nucleic acids from proteins at low ph

Publications (1)

Publication Number Publication Date
DE69941655D1 true DE69941655D1 (de) 2010-01-07

Family

ID=26790014

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69941655T Expired - Lifetime DE69941655D1 (de) 1998-08-04 1999-08-03 Elektrophoretischer Trennung von Nukleinsäuren aus biologischen Proben bei niedrigem pH-Wert

Country Status (6)

Country Link
US (1) US6699986B2 (de)
EP (1) EP0979868B1 (de)
JP (1) JP4531888B2 (de)
AT (1) ATE449843T1 (de)
CA (1) CA2279131C (de)
DE (1) DE69941655D1 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1332972C (zh) * 2002-11-28 2007-08-22 爱科来株式会社 浓缩和纯化核酸的方法和装置
DE60324458D1 (de) * 2002-11-28 2008-12-11 Arkray Inc Verfahren und apparat zum anreichern und zur aufreinigung von nukleinsäure
JP2005110503A (ja) * 2003-10-02 2005-04-28 Arkray Inc 核酸の精製方法及びデバイス
JPWO2005045023A1 (ja) * 2003-11-10 2007-11-29 アークレイ株式会社 核酸の濃縮精製方法および装置
KR100647306B1 (ko) * 2004-12-23 2006-11-23 삼성전자주식회사 아미노기와 카르복실기를 포함하고 제1 ｐＨ에서 양전하를띠는 물질을 이용하여 핵산을 분리하는 방법
US20080085562A1 (en) * 2006-10-10 2008-04-10 Idexx Laboratories, Inc. Detection of gadolinium chelates
US20080085563A1 (en) * 2006-10-10 2008-04-10 Idexx Laboratories, Inc. Detection of Gadolinium Chelates
WO2012144133A1 (ja) * 2011-04-22 2012-10-26 ソニー株式会社 核酸電気泳動方法、核酸濃縮精製方法、核酸電気泳動用カートリッジ、及び該カートリッジの製造方法
EP2773221B1 (de) 2011-11-04 2019-01-09 Bio-Rad Laboratories, Inc. Gleichzeitige reinigung von zellkomponenten
US9766207B2 (en) 2011-11-04 2017-09-19 Bio-Rad Laboratories, Inc. Affinity methods and compositions employing electronic control of pH
EP2814597A4 (de) 2012-02-15 2015-10-28 Bio Rad Laboratories Elektronische steuerung von ph-wert und ionenstärke
US9321012B2 (en) 2012-04-04 2016-04-26 Bio-Rad Laboratories, Inc. Electronic protein fractionation

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4750982A (en) 1985-03-21 1988-06-14 Lifecodes Corp. Process and apparatus for purifying and concentrating DNA from crude mixtures containing DNA
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4822470A (en) * 1987-10-09 1989-04-18 Baylor College Of Medicine Method of and apparatus for cell poration and cell fusion using radiofrequency electrical pulses
IE883226L (en) 1988-10-25 1990-04-25 Dna Prep Galway Ltd S 22 Separation of nucleic acids from protein material using¹cation exchangers
US5386024A (en) 1993-02-10 1995-01-31 Gen-Probe Incorporated Method to prepare nucleic acids from a biological sample using low pH and acid protease
US5635045A (en) * 1993-05-20 1997-06-03 Alam; Aftab Apparatus for, and a method of, electroelution isolation of biomolecules and recovering biomolecules after elution
US5582988A (en) 1994-09-15 1996-12-10 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
US5434270A (en) 1994-09-15 1995-07-18 Eastman Kodak Company Weakly basic polymerizable monomers and polymers prepared therefrom
US5973137A (en) 1996-02-13 1999-10-26 Gentra Systems, Inc. Low pH RNA isolation reagents, method, and kit
DE19700364A1 (de) * 1997-01-08 1998-07-09 Bayer Ag Elektrokinetische Probenvorbereitung

Also Published As

Publication number Publication date
EP0979868B1 (de) 2009-11-25
ATE449843T1 (de) 2009-12-15
CA2279131A1 (en) 2000-02-04
US20010049437A1 (en) 2001-12-06
EP0979868A3 (de) 2001-07-18
CA2279131C (en) 2010-12-14
JP4531888B2 (ja) 2010-08-25
JP2000146911A (ja) 2000-05-26
US6699986B2 (en) 2004-03-02
EP0979868A2 (de) 2000-02-16

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