DE69825904D1 - Mutante alpha-amylase mit zusätzlicher disulfidverbindung - Google Patents

Mutante alpha-amylase mit zusätzlicher disulfidverbindung

Info

Publication number
DE69825904D1
DE69825904D1 DE69825904T DE69825904T DE69825904D1 DE 69825904 D1 DE69825904 D1 DE 69825904D1 DE 69825904 T DE69825904 T DE 69825904T DE 69825904 T DE69825904 T DE 69825904T DE 69825904 D1 DE69825904 D1 DE 69825904D1
Authority
DE
Germany
Prior art keywords
alpha amylase
disulfide compound
additional disulfide
mutant alpha
disclosed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69825904T
Other languages
English (en)
Other versions
DE69825904T2 (de
Inventor
Anthony G Day
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Genencor International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor International Inc filed Critical Genencor International Inc
Publication of DE69825904D1 publication Critical patent/DE69825904D1/de
Application granted granted Critical
Publication of DE69825904T2 publication Critical patent/DE69825904T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Detergent Compositions (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
DE69825904T 1997-07-11 1998-06-29 Mutante alpha-amylase mit zusätzlicher disulfidverbindung Expired - Lifetime DE69825904T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US890383 1997-07-11
US08/890,383 US6008026A (en) 1997-07-11 1997-07-11 Mutant α-amylase having introduced therein a disulfide bond
PCT/US1998/013572 WO1999002702A1 (en) 1997-07-11 1998-06-29 MUTANT α-AMYLASE HAVING INTRODUCED THEREIN A DISULFIDE BOND

Publications (2)

Publication Number Publication Date
DE69825904D1 true DE69825904D1 (de) 2004-09-30
DE69825904T2 DE69825904T2 (de) 2005-09-01

Family

ID=25396599

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69825904T Expired - Lifetime DE69825904T2 (de) 1997-07-11 1998-06-29 Mutante alpha-amylase mit zusätzlicher disulfidverbindung

Country Status (8)

Country Link
US (1) US6008026A (de)
EP (1) EP1002098B1 (de)
JP (1) JP2001509389A (de)
AT (1) ATE274588T1 (de)
AU (1) AU8473898A (de)
CA (1) CA2295693C (de)
DE (1) DE69825904T2 (de)
WO (1) WO1999002702A1 (de)

Families Citing this family (91)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2278931A1 (en) 1997-01-30 1998-08-06 University Of Virginia Patent Foundation Cysteine-depleted peptides recognized by a3-restricted cytotoxic lymphocytes, and uses therefor
DZ3349A1 (fr) * 2000-07-28 2002-02-07 Henkel Kgaa Nouvelle enzyme amylolytique issue de bacillus sp. a 7-7 (dsm 12368) ainsi que produits de lavage et nettoyage contenant ledit enzyme amylolytique
US20020155574A1 (en) * 2000-08-01 2002-10-24 Novozymes A/S Alpha-amylase mutants with altered properties
US6623949B1 (en) 2000-08-04 2003-09-23 Genencor International, Inc. Variant EGIII-like cellulase compositions
US7888104B2 (en) 2000-11-28 2011-02-15 Henkel Ag & Co. Kgaa Cyclodextrin glucanotransferase (CGTase), obtained from<I>Bacillus agaradherens<λ>(DSM 9948) and detergents and cleaning agents containing said novel cyclodextrin glucanotransferase
NZ531208A (en) * 2001-08-31 2005-08-26 Avidex Ltd Multivalent soluble T cell receptor (TCR) complexes
US7638151B2 (en) 2003-03-10 2009-12-29 Danisco Us Inc. Grain compositions containing pre-biotic isomalto-oligosaccharides and methods of making and using same
DE102004047777B4 (de) 2004-10-01 2018-05-09 Basf Se Alpha-Amylase-Varianten mit erhöhter Lösungsmittelstabilität, Verfahren zu deren Herstellung sowie deren Verwendung
US7968318B2 (en) 2006-06-06 2011-06-28 Genencor International, Inc. Process for conversion of granular starch to ethanol
US20080299622A1 (en) * 2007-02-07 2008-12-04 Paulson Bradley A Starch Hydrolysis Using Phytase with an Alpha Amylase
CN101680005A (zh) 2007-03-14 2010-03-24 丹尼斯科美国公司 从大麦产生乙醇和含有降低的β-葡聚糖和肌醇六磷酸的DDGS
EP2250277A1 (de) * 2008-02-06 2010-11-17 Danisco US Inc. Ph-einstellungsfreies system zur herstellung von vergärbaren zuckern und alkohol
BRPI0909143A2 (pt) 2008-03-11 2015-08-04 Danisco Us Inc Métodos de produzir um álcool, de reduzir ácido fítico durante a fermentação de etanol, e de reduzir ácido fítico em grãos secos destilados com materiais solúveis
EP2461702B1 (de) * 2009-08-07 2018-11-21 Danisco US Inc. Alpha-amylase mischungen zur stärke verarbeitung und verwendungsverfahren
WO2012027226A1 (en) 2010-08-24 2012-03-01 Danisco Us Inc. A food product comprising a low temperature rice protein concentrate
MX2011008654A (es) 2010-08-24 2012-02-23 Corn Products Int Inc Produccion de isomaltooligosacaridos y usos para los mismos.
CA2849401A1 (en) 2011-09-29 2013-04-04 Danisco Us Inc. Liquefaction and saccharification of granular starch at high concentration
WO2013148663A1 (en) 2012-03-28 2013-10-03 Danisco Us Inc. Low temperature method for making high glucose syrup
EP2831259A1 (de) 2012-03-28 2015-02-04 Danisco US Inc. Verfahren zur herstellung eines sirups mit hohem maltoseanteil
US20150152457A1 (en) 2012-03-30 2015-06-04 Danisco Us Inc. Direct starch to fermentable sugar
US9315831B2 (en) 2012-03-30 2016-04-19 Danisco Us Inc. Direct starch to fermentable sugar as feedstock for the production of isoprene, isoprenoid precursor molecules, and/or isoprenoids
EP2931872B1 (de) 2012-12-11 2018-01-17 Danisco US Inc. Trichoderma-reesei-wirtszellen zur expression einer glukoamylase aus aspergillus fumigatus und verfahren zur verwendung davon
WO2014194034A2 (en) 2013-05-29 2014-12-04 Danisco Us Inc. Novel metalloproteases
EP3260538B1 (de) 2013-05-29 2021-04-14 Danisco US Inc. Neuartige metalloproteasen
WO2014194117A2 (en) 2013-05-29 2014-12-04 Danisco Us Inc. Novel metalloproteases
WO2014194032A1 (en) 2013-05-29 2014-12-04 Danisco Us Inc. Novel metalloproteases
WO2015089447A1 (en) 2013-12-13 2015-06-18 Danisco Us Inc. Serine proteases of the bacillus gibsonii-clade
EP3080262B1 (de) 2013-12-13 2019-02-06 Danisco US Inc. Serinproteaseenzymen von bacillus
WO2015095046A1 (en) 2013-12-16 2015-06-25 E. I. Du Pont De Nemours And Company Use of poly alpha-1,3-glucan ethers as viscosity modifiers
EP3789407B1 (de) 2013-12-18 2024-07-24 Nutrition & Biosciences USA 4, Inc. Kationische polyalpha-1,3-glucanether
WO2015123323A1 (en) 2014-02-14 2015-08-20 E. I. Du Pont De Nemours And Company Poly-alpha-1,3-1,6-glucans for viscosity modification
WO2015138283A1 (en) 2014-03-11 2015-09-17 E. I. Du Pont De Nemours And Company Oxidized poly alpha-1,3-glucan as detergent builder
DK3119884T3 (da) 2014-03-21 2019-10-14 Danisco Us Inc Serinproteaser af bacillus-arter
US9714403B2 (en) 2014-06-19 2017-07-25 E I Du Pont De Nemours And Company Compositions containing one or more poly alpha-1,3-glucan ether compounds
EP3158043B1 (de) 2014-06-19 2021-03-10 Nutrition & Biosciences USA 4, Inc. Zusammensetzungen mit einer oder mehreren poly-alpha-1,3-glucan-ether-verbindungen
DK3207129T3 (da) 2014-10-17 2020-02-24 Danisco Us Inc Serinproteaser af bacillus-arten
WO2016069548A2 (en) 2014-10-27 2016-05-06 Danisco Us Inc. Serine proteases
WO2016069569A2 (en) 2014-10-27 2016-05-06 Danisco Us Inc. Serine proteases
EP4403631A2 (de) 2014-10-27 2024-07-24 Danisco US Inc. Serinproteasen
US20180010074A1 (en) 2014-10-27 2018-01-11 Danisco Us Inc. Serine proteases of bacillus species
WO2016069552A1 (en) 2014-10-27 2016-05-06 Danisco Us Inc. Serine proteases
CN107109450A (zh) 2014-12-23 2017-08-29 纳幕尔杜邦公司 酶促产生的纤维素
EP3294884B1 (de) 2015-05-13 2021-01-27 Danisco US Inc. Aprl-clade-protease-varianten and verwendungen davon
WO2016201069A1 (en) 2015-06-09 2016-12-15 Danisco Us Inc Low-density enzyme-containing particles
WO2016201040A1 (en) 2015-06-09 2016-12-15 Danisco Us Inc. Water-triggered enzyme suspension
ES2962329T3 (es) 2015-06-09 2024-03-18 Danisco Us Inc Encapsulados de estallido osmótico
EP4234693A3 (de) 2015-06-17 2023-11-01 Danisco US Inc Bacillus-gibboni-clade-serin-proteasen
JP7364330B2 (ja) 2015-11-05 2023-10-18 ダニスコ・ユーエス・インク パエニバチルス（Ｐａｅｎｉｂａｃｉｌｌｕｓ）属種及びバチルス（Ｂａｃｉｌｌｕｓ）属種のマンナナーゼ
EP3371308B1 (de) 2015-11-05 2022-05-11 Danisco US Inc. Paenibacillus sp. mannanasen
JP6997706B2 (ja) 2015-11-13 2022-01-18 ニュートリション・アンド・バイオサイエンシーズ・ユーエスエー・フォー，インコーポレイテッド 洗濯ケアおよび織物ケアにおいて使用するためのグルカン繊維組成物
JP2019504932A (ja) 2015-11-13 2019-02-21 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ 洗濯ケアおよび織物ケアにおいて使用するためのグルカン繊維組成物
EP3374400B1 (de) 2015-11-13 2022-04-13 Nutrition & Biosciences USA 4, Inc. Glucanfaserzusammensetzungen zur verwendung in der wäsche- und textilpflege
CN108779448B (zh) 2015-12-09 2023-08-18 丹尼斯科美国公司 α-淀粉酶组合变体
US20180362946A1 (en) 2015-12-18 2018-12-20 Danisco Us Inc. Polypeptides with endoglucanase activity and uses thereof
CA3022875A1 (en) 2016-05-03 2017-11-09 Danisco Us Inc Protease variants and uses thereof
EP3845642B1 (de) 2016-05-05 2023-08-09 Danisco US Inc. Proteasevarianten und verwendungen davon
WO2017205337A1 (en) 2016-05-23 2017-11-30 Dupont Nutrition Biosciences Aps Baking process and a method thereof
JP2019523645A (ja) 2016-05-31 2019-08-29 ダニスコ・ユーエス・インク プロテアーゼ変異体およびその使用
US11946080B2 (en) 2016-06-17 2024-04-02 Danisco Us Inc. Protease variants and uses thereof
WO2018085524A2 (en) 2016-11-07 2018-05-11 Danisco Us Inc Laundry detergent composition
CN110312795B (zh) 2016-12-21 2024-07-23 丹尼斯科美国公司 蛋白酶变体及其用途
CN110312794B (zh) 2016-12-21 2024-04-12 丹尼斯科美国公司 吉氏芽孢杆菌进化枝丝氨酸蛋白酶
US11453871B2 (en) 2017-03-15 2022-09-27 Danisco Us Inc. Trypsin-like serine proteases and uses thereof
EP3601515A1 (de) 2017-03-31 2020-02-05 Danisco US Inc. Enzymformulierungen mit verzögerter freisetzung für bleichmittelhaltige waschmittel
WO2019006077A1 (en) 2017-06-30 2019-01-03 Danisco Us Inc PARTICLES CONTAINING LOW AGGLOMERATION ENZYME
EP3717643A1 (de) 2017-11-29 2020-10-07 Danisco US Inc. Subtilisin-varianten mit verbesserter stabilität
WO2019125683A1 (en) 2017-12-21 2019-06-27 Danisco Us Inc Enzyme-containing, hot-melt granules comprising a thermotolerant desiccant
MX2020008302A (es) 2018-02-08 2020-10-14 Danisco Us Inc Partículas de matriz de cera térmicamente resistentes para encapsulación de enzimas.
WO2019245705A1 (en) 2018-06-19 2019-12-26 Danisco Us Inc Subtilisin variants
WO2019245704A1 (en) 2018-06-19 2019-12-26 Danisco Us Inc Subtilisin variants
WO2020047215A1 (en) 2018-08-30 2020-03-05 Danisco Us Inc Enzyme-containing granules
JP2022503923A (ja) 2018-09-27 2022-01-12 ダニスコ・ユーエス・インク 医療用器具を洗浄するための組成物
US20230028935A1 (en) 2018-11-28 2023-01-26 Danisco Us Inc Subtilisin variants having improved stability
CN109897842B (zh) * 2019-03-25 2020-12-11 中国农业科学院北京畜牧兽医研究所 淀粉酶突变体zdamya及其编码基因和应用
WO2020242858A1 (en) 2019-05-24 2020-12-03 Danisco Us Inc Subtilisin variants and methods of use
EP3980517A1 (de) 2019-06-06 2022-04-13 Danisco US Inc. Verfahren und zusammensetzungen zum reinigen
CN110283881B (zh) * 2019-06-24 2020-11-06 江南大学 一种提高酶稳定性的方法及其应用
US20240034960A1 (en) 2020-08-27 2024-02-01 Danisco Us Inc Enzymes and enzyme compositions for cleaning
WO2022165107A1 (en) 2021-01-29 2022-08-04 Danisco Us Inc Compositions for cleaning and methods related thereto
CN117616120A (zh) 2021-06-30 2024-02-27 丹尼斯科美国公司 变体脂肪酶及其用途
WO2023034486A2 (en) 2021-09-03 2023-03-09 Danisco Us Inc. Laundry compositions for cleaning
EP4402258A2 (de) 2021-09-13 2024-07-24 Danisco US Inc. Bioaktive granulate
WO2023114932A2 (en) 2021-12-16 2023-06-22 Danisco Us Inc. Subtilisin variants and methods of use
WO2023114936A2 (en) 2021-12-16 2023-06-22 Danisco Us Inc. Subtilisin variants and methods of use
WO2023114939A2 (en) 2021-12-16 2023-06-22 Danisco Us Inc. Subtilisin variants and methods of use
WO2023168234A1 (en) 2022-03-01 2023-09-07 Danisco Us Inc. Enzymes and enzyme compositions for cleaning
WO2023250301A1 (en) 2022-06-21 2023-12-28 Danisco Us Inc. Methods and compositions for cleaning comprising a polypeptide having thermolysin activity
WO2024050339A1 (en) 2022-09-02 2024-03-07 Danisco Us Inc. Mannanase variants and methods of use
WO2024050346A1 (en) 2022-09-02 2024-03-07 Danisco Us Inc. Detergent compositions and methods related thereto
WO2024050343A1 (en) 2022-09-02 2024-03-07 Danisco Us Inc. Subtilisin variants and methods related thereto
WO2024102698A1 (en) 2022-11-09 2024-05-16 Danisco Us Inc. Subtilisin variants and methods of use

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2665178A1 (fr) * 1990-07-30 1992-01-31 Agronomique Inst Nat Rech Variants thermostables de l'alpha-amylase de bacillus licheniformis, leur procede de preparation et leur utilisation.
EP0722490B2 (de) * 1993-10-08 2013-10-23 Novozymes A/S Amylasevarianten
WO1995035382A2 (en) * 1994-06-17 1995-12-28 Genecor International Inc. NOVEL AMYLOLYTIC ENZYMES DERIVED FROM THE B. LICHENIFORMIS α-AMYLASE, HAVING IMPROVED CHARACTERISTICS
EP0815193A1 (de) * 1995-03-24 1998-01-07 Genencor International Inc. Amylase enthaltendes verbessertes textilwasmittel
US5736499A (en) * 1995-06-06 1998-04-07 Genencor International, Inc. Mutant A-amylase

Also Published As

Publication number Publication date
EP1002098B1 (de) 2004-08-25
DE69825904T2 (de) 2005-09-01
CA2295693A1 (en) 1999-01-21
US6008026A (en) 1999-12-28
ATE274588T1 (de) 2004-09-15
AU8473898A (en) 1999-02-08
JP2001509389A (ja) 2001-07-24
WO1999002702A1 (en) 1999-01-21
EP1002098A1 (de) 2000-05-24
CA2295693C (en) 2007-07-31

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