DE69731112D1 - Verfahren zur synthese von phosphorothioat-oligonukelotiden - Google Patents
Verfahren zur synthese von phosphorothioat-oligonukelotidenInfo
- Publication number
- DE69731112D1 DE69731112D1 DE69731112T DE69731112T DE69731112D1 DE 69731112 D1 DE69731112 D1 DE 69731112D1 DE 69731112 T DE69731112 T DE 69731112T DE 69731112 T DE69731112 T DE 69731112T DE 69731112 D1 DE69731112 D1 DE 69731112D1
- Authority
- DE
- Germany
- Prior art keywords
- oligonucelotides
- synthesising
- phosphorothioate
- methods
- novel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Saccharide Compounds (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US775011 | 1996-12-27 | ||
US08/775,011 US6172217B1 (en) | 1996-12-27 | 1996-12-27 | Method of synthesizing phosphorothioate oligonucleotides |
PCT/US1997/023601 WO1998029427A1 (en) | 1996-12-27 | 1997-12-12 | Method of synthesizing phosphorothioate oligonucleotides |
Publications (2)
Publication Number | Publication Date |
---|---|
DE69731112D1 true DE69731112D1 (de) | 2004-11-11 |
DE69731112T2 DE69731112T2 (de) | 2006-02-23 |
Family
ID=25103044
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69731112T Expired - Fee Related DE69731112T2 (de) | 1996-12-27 | 1997-12-12 | Verfahren zur synthese von phosphorothioat-oligonukelotiden |
Country Status (9)
Country | Link |
---|---|
US (4) | US6172217B1 (de) |
EP (1) | EP0948512B1 (de) |
JP (1) | JP2000511933A (de) |
KR (1) | KR100458979B1 (de) |
AT (1) | ATE278702T1 (de) |
AU (1) | AU721750B2 (de) |
CA (1) | CA2276213C (de) |
DE (1) | DE69731112T2 (de) |
WO (1) | WO1998029427A1 (de) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6172217B1 (en) * | 1996-12-27 | 2001-01-09 | Isis Pharmaceuticals Inc. | Method of synthesizing phosphorothioate oligonucleotides |
FR2803848B1 (fr) * | 2000-01-19 | 2002-02-15 | Adir | Nouveaux derives de benzenesulfonamide, leur procede de preparation et les compositions pharmaceutiques qui les contiennent |
DE10041539A1 (de) * | 2000-08-24 | 2002-03-07 | Febit Ferrarius Biotech Gmbh | Neue Amiditderivate zur Synthese von Polymeren auf Oberflächen |
US20040198640A1 (en) * | 2003-04-02 | 2004-10-07 | Dharmacon, Inc. | Stabilized polynucleotides for use in RNA interference |
KR101147147B1 (ko) * | 2004-04-01 | 2012-05-25 | 머크 샤프 앤드 돔 코포레이션 | Rna 간섭의 오프 타겟 효과 감소를 위한 변형된폴리뉴클레오타이드 |
US7923207B2 (en) | 2004-11-22 | 2011-04-12 | Dharmacon, Inc. | Apparatus and system having dry gene silencing pools |
US7935811B2 (en) | 2004-11-22 | 2011-05-03 | Dharmacon, Inc. | Apparatus and system having dry gene silencing compositions |
US7923206B2 (en) * | 2004-11-22 | 2011-04-12 | Dharmacon, Inc. | Method of determining a cellular response to a biological agent |
US8252755B2 (en) | 2006-09-22 | 2012-08-28 | Dharmacon, Inc. | Duplex oligonucleotide complexes and methods for gene silencing by RNA interference |
KR100886139B1 (ko) * | 2007-11-13 | 2009-02-27 | 주식회사 삼천리제약 | 올리고뉴클레오타이드의 제조방법 |
US8188060B2 (en) | 2008-02-11 | 2012-05-29 | Dharmacon, Inc. | Duplex oligonucleotides with enhanced functionality in gene regulation |
Family Cites Families (34)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3687808A (en) | 1969-08-14 | 1972-08-29 | Univ Leland Stanford Junior | Synthetic polynucleotides |
US4500707A (en) | 1980-02-29 | 1985-02-19 | University Patents, Inc. | Nucleosides useful in the preparation of polynucleotides |
US4458066A (en) | 1980-02-29 | 1984-07-03 | University Patents, Inc. | Process for preparing polynucleotides |
US5132418A (en) | 1980-02-29 | 1992-07-21 | University Patents, Inc. | Process for preparing polynucleotides |
US4668777A (en) | 1981-03-27 | 1987-05-26 | University Patents, Inc. | Phosphoramidite nucleoside compounds |
US4973679A (en) | 1981-03-27 | 1990-11-27 | University Patents, Inc. | Process for oligonucleo tide synthesis using phosphormidite intermediates |
US4415732A (en) | 1981-03-27 | 1983-11-15 | University Patents, Inc. | Phosphoramidite compounds and processes |
DE3329892A1 (de) | 1983-08-18 | 1985-03-07 | Köster, Hubert, Prof. Dr., 2000 Hamburg | Verfahren zur herstellung von oligonucleotiden |
USRE34069E (en) | 1983-08-18 | 1992-09-15 | Biosyntech Gmbh | Process for the preparation of oligonucleotides |
GB8607265D0 (en) * | 1986-03-24 | 1986-04-30 | London King S College | Protecting groups of organic synthesis |
EP0339842B1 (de) | 1988-04-27 | 1996-11-06 | Isis Pharmaceuticals, Inc. | Oligoribonukleotid-Derivate und ihre Anwendung als antivirale Mittel |
US5449769A (en) | 1989-03-06 | 1995-09-12 | Gen-Probe Incorporated | Method and reagent for sulfurization of organophosphorous compounds |
DK0497875T3 (da) | 1989-10-24 | 2000-07-03 | Gilead Sciences Inc | 2'-modificerede oligonukleotider |
JP2580091B2 (ja) | 1990-01-11 | 1997-02-12 | アイシス・ファーマシューティカルス・インコーポレーテッド | Rna活性および遺伝子発現を検出および変調するための組成物および方法 |
US5506212A (en) | 1990-01-11 | 1996-04-09 | Isis Pharmaceuticals, Inc. | Oligonucleotides with substantially chirally pure phosphorothioate linkages |
US5635488A (en) | 1991-10-15 | 1997-06-03 | Isis Pharmaceuticals, Inc. | Compounds having phosphorodithioate linkages of high chiral purity |
US5212295A (en) | 1990-01-11 | 1993-05-18 | Isis Pharmaceuticals | Monomers for preparation of oligonucleotides having chiral phosphorus linkages |
US5166387A (en) | 1990-01-12 | 1992-11-24 | Applied Biosystems, Inc. | Method of synthesizing sulfurized oligonucleotide analogs with thiuram disulfides |
US5292875A (en) | 1990-04-20 | 1994-03-08 | Lynx Therapeutics, Inc. | Method of synthesizing sulfurized oligonucleotide analogs |
US5151510A (en) | 1990-04-20 | 1992-09-29 | Applied Biosystems, Inc. | Method of synethesizing sulfurized oligonucleotide analogs |
US5672697A (en) * | 1991-02-08 | 1997-09-30 | Gilead Sciences, Inc. | Nucleoside 5'-methylene phosphonates |
US5512668A (en) | 1991-03-06 | 1996-04-30 | Polish Academy Of Sciences | Solid phase oligonucleotide synthesis using phospholane intermediates |
US5525719A (en) * | 1991-08-30 | 1996-06-11 | Chemgenes Corporation | N-protected-2'-O-methyl-and N-protected-3'-O-methyl-ribonucleosides and their phosphoramidite derivatives |
GB9127243D0 (en) | 1991-12-23 | 1992-02-19 | King S College London | Synthesis of phosphorothioate analogues of oligonucleotides |
JPH08507752A (ja) | 1993-01-08 | 1996-08-20 | ハイブライドン インコーポレイテッド | 二量体ブロックの合成と二量体ブロックを使ったオリゴヌクレオチド結合法 |
US6015886A (en) * | 1993-05-24 | 2000-01-18 | Chemgenes Corporation | Oligonucleotide phosphate esters |
US5571902A (en) | 1993-07-29 | 1996-11-05 | Isis Pharmaceuticals, Inc. | Synthesis of oligonucleotides |
JPH07157498A (ja) | 1993-12-07 | 1995-06-20 | Sankyo Co Ltd | 短鎖ホスホロチオエート結合オリゴデオキシヌクレオチド |
WO1995026204A1 (en) | 1994-03-25 | 1995-10-05 | Isis Pharmaceuticals, Inc. | Immune stimulation by phosphorothioate oligonucleotide analogs |
US5405985A (en) | 1994-07-08 | 1995-04-11 | The Goodyear Tire & Rubber Company | Preparation of sulfur-containing organosilicon compounds |
DE4438918A1 (de) * | 1994-11-04 | 1996-05-09 | Hoechst Ag | Modifizierte Oligonukleotide, deren Herstellung sowie deren Verwendung |
WO1996030386A1 (fr) | 1995-03-31 | 1996-10-03 | Drug Delivery System Institute, Ltd. | Derives d'amidite et derives d'oligonucleotides |
US6172217B1 (en) * | 1996-12-27 | 2001-01-09 | Isis Pharmaceuticals Inc. | Method of synthesizing phosphorothioate oligonucleotides |
US6649750B1 (en) * | 2000-01-05 | 2003-11-18 | Isis Pharmaceuticals, Inc. | Process for the preparation of oligonucleotide compounds |
-
1996
- 1996-12-27 US US08/775,011 patent/US6172217B1/en not_active Expired - Fee Related
-
1997
- 1997-12-12 AT AT97954597T patent/ATE278702T1/de not_active IP Right Cessation
- 1997-12-12 DE DE69731112T patent/DE69731112T2/de not_active Expired - Fee Related
- 1997-12-12 KR KR10-1999-7005779A patent/KR100458979B1/ko not_active IP Right Cessation
- 1997-12-12 JP JP10530137A patent/JP2000511933A/ja not_active Ceased
- 1997-12-12 EP EP97954597A patent/EP0948512B1/de not_active Expired - Lifetime
- 1997-12-12 CA CA002276213A patent/CA2276213C/en not_active Expired - Fee Related
- 1997-12-12 AU AU59009/98A patent/AU721750B2/en not_active Ceased
- 1997-12-12 WO PCT/US1997/023601 patent/WO1998029427A1/en active IP Right Grant
-
2001
- 2001-01-08 US US09/756,354 patent/US6403781B2/en not_active Expired - Fee Related
-
2002
- 2002-02-06 US US10/068,604 patent/US20020128466A1/en not_active Abandoned
-
2003
- 2003-01-17 US US10/346,659 patent/US6780989B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
CA2276213A1 (en) | 1998-07-09 |
KR20000069706A (ko) | 2000-11-25 |
EP0948512B1 (de) | 2004-10-06 |
EP0948512A1 (de) | 1999-10-13 |
US6172217B1 (en) | 2001-01-09 |
WO1998029427A1 (en) | 1998-07-09 |
ATE278702T1 (de) | 2004-10-15 |
CA2276213C (en) | 2003-12-02 |
AU5900998A (en) | 1998-07-31 |
US20010003132A1 (en) | 2001-06-07 |
US6780989B2 (en) | 2004-08-24 |
US6403781B2 (en) | 2002-06-11 |
JP2000511933A (ja) | 2000-09-12 |
AU721750B2 (en) | 2000-07-13 |
US20030114662A1 (en) | 2003-06-19 |
EP0948512A4 (de) | 2001-10-24 |
KR100458979B1 (ko) | 2004-12-03 |
US20020128466A1 (en) | 2002-09-12 |
DE69731112T2 (de) | 2006-02-23 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition | ||
8339 | Ceased/non-payment of the annual fee |