DE69637709D1 - Durch elektroporation vermittelter molekulartransfer in intakten pflanzen - Google Patents
Durch elektroporation vermittelter molekulartransfer in intakten pflanzenInfo
- Publication number
- DE69637709D1 DE69637709D1 DE69637709T DE69637709T DE69637709D1 DE 69637709 D1 DE69637709 D1 DE 69637709D1 DE 69637709 T DE69637709 T DE 69637709T DE 69637709 T DE69637709 T DE 69637709T DE 69637709 D1 DE69637709 D1 DE 69637709D1
- Authority
- DE
- Germany
- Prior art keywords
- molecular
- plant
- electroporation
- transfer
- intact plants
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Cell Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Compounds Of Unknown Constitution (AREA)
- Battery Electrode And Active Subsutance (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/517,914 US5859327A (en) | 1995-08-22 | 1995-08-22 | Electroporation-mediated molecular transfer in intact plants |
PCT/US1996/013569 WO1997007666A1 (en) | 1995-08-22 | 1996-08-22 | Electroporation-mediated molecular transfer in intact plants |
Publications (1)
Publication Number | Publication Date |
---|---|
DE69637709D1 true DE69637709D1 (de) | 2008-11-20 |
Family
ID=24061752
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69637709T Expired - Fee Related DE69637709D1 (de) | 1995-08-22 | 1996-08-22 | Durch elektroporation vermittelter molekulartransfer in intakten pflanzen |
Country Status (6)
Country | Link |
---|---|
US (1) | US5859327A (de) |
EP (1) | EP0876095B1 (de) |
JP (1) | JPH11511974A (de) |
AT (1) | ATE410054T1 (de) |
DE (1) | DE69637709D1 (de) |
WO (1) | WO1997007666A1 (de) |
Families Citing this family (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20040229363A1 (en) * | 1998-06-24 | 2004-11-18 | Ed Nolan | High efficiency transfection based on low electric field strength, long pulse length |
US6800484B2 (en) * | 1998-06-24 | 2004-10-05 | Genetronics, Inc. | High efficiency transfection based on low electric field strength, long pulse length |
US6326177B1 (en) | 1999-08-04 | 2001-12-04 | Eastern Virginia Medical School Of The Medical College Of Hampton Roads | Method and apparatus for intracellular electro-manipulation |
SE0003841D0 (sv) * | 2000-10-20 | 2000-10-20 | Daniel Chiu | A method and apparatus for penetration of lipid bilayer membranes |
US7101703B2 (en) | 2001-03-09 | 2006-09-05 | Cornell Research Foundation, Inc. | Electrofusion microelectrode |
WO2003047684A2 (en) * | 2001-12-04 | 2003-06-12 | University Of Southern California | Method for intracellular modifications within living cells using pulsed electric fields |
US7245963B2 (en) * | 2002-03-07 | 2007-07-17 | Advisys, Inc. | Electrode assembly for constant-current electroporation and use |
US8209006B2 (en) * | 2002-03-07 | 2012-06-26 | Vgx Pharmaceuticals, Inc. | Constant current electroporation device and methods of use |
US7329798B2 (en) * | 2002-06-28 | 2008-02-12 | University Of Guelph | Harvest-inducible regulatory elements and methods of using same |
WO2004002216A2 (en) | 2002-06-28 | 2004-01-08 | University Of Guelph | Harvest-inducible genes from alfalfa (medicago sativa) and methods of use thereof |
CN1668752A (zh) * | 2002-07-16 | 2005-09-14 | 独立行政法人农业生物资源研究所 | 包括减压/增压的使用的电穿孔方法 |
US7915044B2 (en) * | 2005-12-22 | 2011-03-29 | Cornell Research Foundation, Inc. | Electrofusion microelectrode and methods of using it to manipulate cells and/or cellular components |
RU2636399C1 (ru) * | 2008-04-30 | 2017-11-23 | Ксилеко, Инк. | Переработка биомассы |
JP2011234648A (ja) * | 2010-05-07 | 2011-11-24 | National Institute Of Biomedical Innovation | 植物形質転換体の作出方法、及び、植物形質転換体 |
US10233419B2 (en) | 2016-06-30 | 2019-03-19 | Zymergen Inc. | Apparatuses and methods for electroporation |
FR3116903A1 (fr) * | 2020-11-27 | 2022-06-03 | Vegetal Signals | Dispositif d’analyse électrophysiologique d’au moins une plante |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4407956A (en) | 1981-03-13 | 1983-10-04 | The Regents Of The University Of California | Cloned cauliflower mosaic virus DNA as a plant vehicle |
US5371003A (en) * | 1987-05-05 | 1994-12-06 | Sandoz Ltd. | Electrotransformation process |
US4822470A (en) | 1987-10-09 | 1989-04-18 | Baylor College Of Medicine | Method of and apparatus for cell poration and cell fusion using radiofrequency electrical pulses |
US4832814A (en) | 1987-12-28 | 1989-05-23 | E. I. Du Pont De Nemours And Company | Electrofusion cell and method of making the same |
US5236835A (en) * | 1988-10-05 | 1993-08-17 | Hapgood, C.V. | Electro insertion of proteins into red cell membranes |
US5286634A (en) | 1989-09-28 | 1994-02-15 | Stadler Joan K | Synergistic method for host cell transformation |
US5384253A (en) * | 1990-12-28 | 1995-01-24 | Dekalb Genetics Corporation | Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes |
JPH07505531A (ja) * | 1992-04-15 | 1995-06-22 | プラント・ジェネティック・システムズ・エヌ・ブイ | 単子葉植物細胞の形質転換法 |
-
1995
- 1995-08-22 US US08/517,914 patent/US5859327A/en not_active Expired - Fee Related
-
1996
- 1996-08-22 JP JP9510422A patent/JPH11511974A/ja not_active Ceased
- 1996-08-22 AT AT96930566T patent/ATE410054T1/de not_active IP Right Cessation
- 1996-08-22 WO PCT/US1996/013569 patent/WO1997007666A1/en active Application Filing
- 1996-08-22 EP EP96930566A patent/EP0876095B1/de not_active Expired - Lifetime
- 1996-08-22 DE DE69637709T patent/DE69637709D1/de not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
WO1997007666A1 (en) | 1997-03-06 |
US5859327A (en) | 1999-01-12 |
EP0876095A1 (de) | 1998-11-11 |
EP0876095A4 (de) | 2001-09-19 |
JPH11511974A (ja) | 1999-10-19 |
EP0876095B1 (de) | 2008-10-08 |
ATE410054T1 (de) | 2008-10-15 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition | ||
8339 | Ceased/non-payment of the annual fee |