DE69637359D1 - Nucleinsäuresequenz xlnR des Regulators XylR des xylanolytischen Biosyntheseweges aus Aspergillus niger - Google Patents

Nucleinsäuresequenz xlnR des Regulators XylR des xylanolytischen Biosyntheseweges aus Aspergillus niger

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Publication number
DE69637359D1
DE69637359D1 DE69637359T DE69637359T DE69637359D1 DE 69637359 D1 DE69637359 D1 DE 69637359D1 DE 69637359 T DE69637359 T DE 69637359T DE 69637359 T DE69637359 T DE 69637359T DE 69637359 D1 DE69637359 D1 DE 69637359D1
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Germany
Prior art keywords
nucleic acid
mutants
gene
acid sequence
regulator
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Expired - Lifetime
Application number
DE69637359T
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English (en)
Other versions
DE69637359T2 (de
Inventor
Graaff Leendert Fungal Geno De
Jacob Visser
Den Broeck Henriette Catha Van
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DuPont Nutrition Biosciences ApS
Original Assignee
Danisco AS
Danisco US Inc
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Application filed by Danisco AS, Danisco US Inc filed Critical Danisco AS
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Publication of DE69637359D1 publication Critical patent/DE69637359D1/de
Publication of DE69637359T2 publication Critical patent/DE69637359T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/38—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C07K14/4705—Regulators; Modulating activity stimulating, promoting or activating activity
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01015—Polygalacturonase (3.2.1.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01037—Xylan 1,4-beta-xylosidase (3.2.1.37)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01055—Alpha-N-arabinofuranosidase (3.2.1.55)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01099—Arabinan endo-1,5-alpha-L-arabinosidase (3.2.1.99)

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  • Health & Medical Sciences (AREA)
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  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Toxicology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
DE69637359T 1995-06-23 1996-06-24 Nucleinsäuresequenz xlnR des Regulators XylR des xylanolytischen Biosyntheseweges aus Aspergillus niger Expired - Lifetime DE69637359T2 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP95201707 1995-06-23
EP95201707 1995-06-23
EP95202346 1995-08-30
EP95202346 1995-08-30

Publications (2)

Publication Number Publication Date
DE69637359D1 true DE69637359D1 (de) 2008-01-17
DE69637359T2 DE69637359T2 (de) 2008-10-30

Family

ID=26139424

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69637359T Expired - Lifetime DE69637359T2 (de) 1995-06-23 1996-06-24 Nucleinsäuresequenz xlnR des Regulators XylR des xylanolytischen Biosyntheseweges aus Aspergillus niger
DE69637908T Expired - Lifetime DE69637908D1 (de) 1995-06-23 1996-06-24 Neuartige methode zur isolierung von mutanten und zur klonierung der complementierenden gene

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE69637908T Expired - Lifetime DE69637908D1 (de) 1995-06-23 1996-06-24 Neuartige methode zur isolierung von mutanten und zur klonierung der complementierenden gene

Country Status (14)

Country Link
US (3) US6177261B1 (de)
EP (2) EP1514936B1 (de)
JP (2) JP4339400B2 (de)
CN (2) CN1661014B (de)
AT (2) ATE429499T1 (de)
AU (1) AU714511B2 (de)
BR (1) BR9608910A (de)
CA (2) CA2224626C (de)
DE (2) DE69637359T2 (de)
DK (2) DK0833922T3 (de)
NZ (1) NZ311181A (de)
PL (2) PL185052B1 (de)
PT (1) PT833922E (de)
WO (1) WO1997000962A1 (de)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT833922E (pt) * 1995-06-23 2009-07-27 Danisco Novo método de isolamento de mutantes e de clonagem do gene complementar
US6391583B1 (en) * 1998-12-18 2002-05-21 Wisconsin Alumni Research Foundation Method of producing antihypercholesterolemic agents
AU2001278496A1 (en) * 2000-07-21 2002-02-05 Syngenta Participations Ag Zinc finger domain recognition code and uses thereof
KR100428998B1 (ko) * 2001-09-10 2004-04-28 한국과학기술원 모체 단백질과는 크기와 서열이 다른 단백질의 변이집단을생산하는 방법
EP2009093A3 (de) * 2004-09-22 2009-04-08 Bioworks, Inc. Transgene Trichoderm-Stämme und ihre Verwendung bei der Biokontrolle
GB0424940D0 (en) 2004-11-11 2004-12-15 Danisco Transcription factors
KR100801960B1 (ko) 2006-11-14 2008-02-12 건국대학교 산학협력단 ｍＲＮＡ 결합 단백질 ＸａｉＦ, 이를 부착하는 유전자구조물 그리고 이들을 이용한 외래 유전자의 과발현 방법
EP2350272A4 (de) * 2008-11-03 2013-01-16 Iogen Energy Corp Wirte und fermentierungsverfahren zur zellulaseherstellung
WO2010129287A2 (en) 2009-04-27 2010-11-11 The Board Of Trustees Of The University Of Illinois Hemicellulose-degrading enzymes
EP2508600B1 (de) * 2009-12-05 2015-08-12 Amano Enzyme Inc. Mutantes enzym und seine anwendung
WO2012030844A1 (en) 2010-08-30 2012-03-08 Novozymes A/S Polypeptides having endoglucanase activity and polynucleotides encoding same
EP2611901B1 (de) 2010-08-30 2016-05-11 Novozymes A/S Polypeptide mit beta-glucosidase-aktivität, beta-xylosidase-aktivität oder beta-glucosidase- und beta-xylosidase-aktivität sowie dafür kodierende polynukleotide
EP3470514A1 (de) 2010-08-30 2019-04-17 Novozymes A/S Polypeptide mit verstärkter zelluloseabbauender wirkung und dafür kodierende polynukleotide
WO2012030811A1 (en) 2010-08-30 2012-03-08 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
US8624082B2 (en) 2010-08-30 2014-01-07 Novozymes A/S Polypeptides having xylanase activity and polynucleotides encoding same
WO2013159710A1 (zh) * 2012-04-25 2013-10-31 中国科学院上海生命科学研究院 一种提高拜氏梭菌木糖消耗率的方法
WO2017170918A1 (ja) 2016-03-31 2017-10-05 東レ株式会社 変異型ｂｘｌ１遺伝子を有するトリコデルマ属真菌及びそれを使用したキシロオリゴ糖とグルコースの製造方法
CN108315313A (zh) * 2018-03-21 2018-07-24 中国农业科学院饲料研究所 多聚半乳糖醛酸酶及其突变体TePG28b_N85E/S86W和应用
EP3844179A4 (de) * 2018-08-31 2022-08-31 Genomatica, Inc. Xylr-mutante zur verbesserten xylosenutzung oder zur verbesserten co-nutzung von glucose und xylose
CN116355881B (zh) * 2023-03-10 2024-02-23 云南师范大学 酸耐受性提高的β-木糖苷酶突变体D395G及其应用
CN116515841B (zh) * 2023-06-21 2023-09-05 中国科学院天津工业生物技术研究所 一种真菌来源的启动子元件及其应用

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Publication number Priority date Publication date Assignee Title
US5028530A (en) * 1985-01-28 1991-07-02 Xoma Corporation AraB promoters and method of producing polypeptides, including cecropins, by microbiological techniques
DK160947C (da) * 1986-04-17 1991-10-21 Novo Industri As Gen-udtrykkelsessystem
US5264350A (en) 1986-08-18 1993-11-23 Institut Pasteur DNA sequence performing a function which is expressed in an over-production of extracellular proteins by various strains of bacillus, and vectors containing this sequence
GB8807939D0 (en) * 1988-04-05 1988-05-05 Univ Brunel Regulatory cassette for expression vectors
US5464758A (en) * 1993-06-14 1995-11-07 Gossen; Manfred Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters
DE705334T1 (de) 1993-06-14 1999-12-30 Basf Ag Strenge kontrolle der genexpression in eukaryotischen zellen durch auf tetrazyklin ansprechende promotoren
ATE238425T1 (de) 1993-07-23 2003-05-15 Dsm Nv Selektionmarker-genfreie rekombinante stämme: verfahren zur ihrer herstellung und die verwendung dieser stämme
PT833922E (pt) 1995-06-23 2009-07-27 Danisco Novo método de isolamento de mutantes e de clonagem do gene complementar

Also Published As

Publication number Publication date
PL324186A1 (en) 1998-05-11
EP1514936A3 (de) 2005-04-13
DE69637908D1 (de) 2009-06-04
JP2007244387A (ja) 2007-09-27
WO1997000962A1 (en) 1997-01-09
EP0833922B1 (de) 2009-04-22
PL185052B1 (pl) 2003-02-28
EP1514936B1 (de) 2007-12-05
CA2672126A1 (en) 1997-01-09
CN1207128A (zh) 1999-02-03
CA2224626A1 (en) 1997-01-09
DK1514936T3 (da) 2008-04-14
DE69637359T2 (de) 2008-10-30
AU714511B2 (en) 2000-01-06
PT833922E (pt) 2009-07-27
PL184463B1 (pl) 2002-11-29
US6599745B1 (en) 2003-07-29
EP0833922A1 (de) 1998-04-08
JPH11507838A (ja) 1999-07-13
US20030175893A1 (en) 2003-09-18
AU6244396A (en) 1997-01-22
ATE429499T1 (de) 2009-05-15
CN1661014A (zh) 2005-08-31
CA2672126C (en) 2013-01-08
JP4339901B2 (ja) 2009-10-07
CN1661014B (zh) 2013-05-29
ATE380242T1 (de) 2007-12-15
EP1514936A2 (de) 2005-03-16
US6177261B1 (en) 2001-01-23
CN1192106C (zh) 2005-03-09
JP4339400B2 (ja) 2009-10-07
US8461320B2 (en) 2013-06-11
CA2224626C (en) 2011-11-01
NZ311181A (en) 2000-02-28
BR9608910A (pt) 1999-05-04
DK0833922T3 (da) 2009-08-03

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