DE69636080D1 - A cascading amplification reaction of nucleic acids - Google Patents

A cascading amplification reaction of nucleic acids

Info

Publication number
DE69636080D1
DE69636080D1 DE69636080T DE69636080T DE69636080D1 DE 69636080 D1 DE69636080 D1 DE 69636080D1 DE 69636080 T DE69636080 T DE 69636080T DE 69636080 T DE69636080 T DE 69636080T DE 69636080 D1 DE69636080 D1 DE 69636080D1
Authority
DE
Germany
Prior art keywords
oligonucleotide
nucleic acids
amplification reaction
circular
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69636080T
Other languages
German (de)
Other versions
DE69636080T2 (en
Inventor
Erland Koch
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Olink AB
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Application granted granted Critical
Publication of DE69636080D1 publication Critical patent/DE69636080D1/en
Publication of DE69636080T2 publication Critical patent/DE69636080T2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Steroid Compounds (AREA)
  • Ceramic Products (AREA)

Abstract

A DNA template consisting of multiple tandem repetitions of an oligonucleotide unit is produced by endless copying of a circular oligonucleotide comprising at least one copy of said oligonucleotide unit by means of a nucleic acid polymerase, which is capable of strand displacement and is substantially without 5'-3' exonuclease activity, in the presence of the necessary nucleoside triphosphates and, if necessary, a primer capable of binding to some portion of the oligonucleotide. This reaction can be used in a process for the amplification of a particular DNA sequence wherein said DNA sequence is either circularized or inserted into a circular oligonucleotide, and the resulting circular DNA is used as a template for an endless copying process.
DE69636080T 1995-12-05 1996-12-05 A cascading amplification reaction of nucleic acids Expired - Lifetime DE69636080T2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK137995 1995-12-05
DK137995 1995-12-05

Publications (2)

Publication Number Publication Date
DE69636080D1 true DE69636080D1 (en) 2006-06-01
DE69636080T2 DE69636080T2 (en) 2006-11-30

Family

ID=8104224

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69636080T Expired - Lifetime DE69636080T2 (en) 1995-12-05 1996-12-05 A cascading amplification reaction of nucleic acids
DE69627698T Expired - Lifetime DE69627698T2 (en) 1995-12-05 1996-12-05 A CASCADED REPLACEMENT REACTION OF NUCLEIC ACIDS

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE69627698T Expired - Lifetime DE69627698T2 (en) 1995-12-05 1996-12-05 A CASCADED REPLACEMENT REACTION OF NUCLEIC ACIDS

Country Status (9)

Country Link
US (1) US6610481B2 (en)
EP (2) EP1300466B1 (en)
AT (2) ATE238434T1 (en)
AU (1) AU704750B2 (en)
CA (1) CA2239287C (en)
DE (2) DE69636080T2 (en)
DK (2) DK0868530T3 (en)
ES (1) ES2262902T3 (en)
WO (1) WO1997020948A1 (en)

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DK0862656T3 (en) 1995-11-21 2001-04-09 Univ Yale Unimolecular segment amplification and detection
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JP2002525078A (en) * 1998-09-15 2002-08-13 イェール ユニバーシティ Artificial long terminal repeat
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US6368801B1 (en) 2000-04-12 2002-04-09 Molecular Staging, Inc. Detection and amplification of RNA using target-mediated ligation of DNA by RNA ligase
DE60130763T2 (en) 2000-06-30 2008-07-17 Qiagen Gmbh SIGNAL AMPLIFICATION WITH "LOLLIPOP" HYDRIDIZATION SAMPLES
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US6573051B2 (en) 2001-03-09 2003-06-03 Molecular Staging, Inc. Open circle probes with intramolecular stem structures
WO2003002761A1 (en) * 2001-06-29 2003-01-09 Novozymes A/S Method of multimerization and site-directed mutagenesis
GB2378245A (en) 2001-08-03 2003-02-05 Mats Nilsson Nucleic acid amplification method
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WO2004048594A2 (en) 2002-11-21 2004-06-10 Epicentre Technologies Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
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US7989166B2 (en) 2005-04-12 2011-08-02 In Situ Rcp A/S Circle probes and their use in the identification of biomolecules
CN101213310A (en) 2005-04-12 2008-07-02 Novia公司名下的现场Rcp公司 Methods for production of oligonucleotides
WO2006138284A2 (en) 2005-06-15 2006-12-28 Callida Genomics, Inc. Nucleic acid analysis by random mixtures of non-overlapping fragments
EP1762627A1 (en) 2005-09-09 2007-03-14 Qiagen GmbH Method for the activation of a nucleic acid for performing a polymerase reaction
EP2377929A1 (en) 2005-10-06 2011-10-19 Lucigen Corporation Thermostable viral polymerases and methods of use
US7960104B2 (en) * 2005-10-07 2011-06-14 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
WO2007133831A2 (en) 2006-02-24 2007-11-22 Callida Genomics, Inc. High throughput genome sequencing on dna arrays
US20070168197A1 (en) * 2006-01-18 2007-07-19 Nokia Corporation Audio coding
SG10201405158QA (en) * 2006-02-24 2014-10-30 Callida Genomics Inc High throughput genome sequencing on dna arrays
GB0621361D0 (en) * 2006-10-26 2006-12-06 Fermentas Uab Use of DNA polymerases
US7910354B2 (en) 2006-10-27 2011-03-22 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US20090111705A1 (en) 2006-11-09 2009-04-30 Complete Genomics, Inc. Selection of dna adaptor orientation by hybrid capture
US20080221832A1 (en) * 2006-11-09 2008-09-11 Complete Genomics, Inc. Methods for computing positional base probabilities using experminentals base value distributions
RU2414510C2 (en) * 2007-06-14 2011-03-20 Институт биохимии и генетики Уфимского научного центра РАН Method of amplifying specific nucleic acid fragments through recurrent chain reaction
US8617811B2 (en) 2008-01-28 2013-12-31 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
US8415099B2 (en) * 2007-11-05 2013-04-09 Complete Genomics, Inc. Efficient base determination in sequencing reactions
US8518640B2 (en) * 2007-10-29 2013-08-27 Complete Genomics, Inc. Nucleic acid sequencing and process
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WO2009094583A1 (en) * 2008-01-23 2009-07-30 Complete Genomics, Inc. Methods and compositions for preventing bias in amplification and sequencing reactions
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US10174366B2 (en) 2012-11-14 2019-01-08 Olink Bioscience Ab Localised RCA-based amplification method
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KR20230166141A (en) 2016-12-09 2023-12-06 얼티뷰, 인크. Improved methods for multiplex imaging using labeled nucleic acid imaging agents
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Also Published As

Publication number Publication date
DE69627698T2 (en) 2004-01-15
EP1300466A2 (en) 2003-04-09
EP1300466B1 (en) 2006-04-26
US6610481B2 (en) 2003-08-26
DK1300466T3 (en) 2006-08-28
CA2239287A1 (en) 1997-06-12
ATE324464T1 (en) 2006-05-15
AU704750B2 (en) 1999-05-06
EP0868530B1 (en) 2003-04-23
US20020031764A1 (en) 2002-03-14
CA2239287C (en) 2008-02-26
WO1997020948A1 (en) 1997-06-12
AU7691796A (en) 1997-06-27
EP0868530A1 (en) 1998-10-07
ES2262902T3 (en) 2006-12-01
DK0868530T3 (en) 2003-07-21
ATE238434T1 (en) 2003-05-15
DE69636080T2 (en) 2006-11-30
DE69627698D1 (en) 2003-05-28
EP1300466A3 (en) 2004-01-02

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Owner name: OLINK AB, UPPSALA, SE