DE69634696D1 - Verfahren und testsystem zur hybridisierungsanalyse unter verwendung von peptidnukleinsäure-sonden - Google Patents

Verfahren und testsystem zur hybridisierungsanalyse unter verwendung von peptidnukleinsäure-sonden

Info

Publication number
DE69634696D1
DE69634696D1 DE69634696T DE69634696T DE69634696D1 DE 69634696 D1 DE69634696 D1 DE 69634696D1 DE 69634696 T DE69634696 T DE 69634696T DE 69634696 T DE69634696 T DE 69634696T DE 69634696 D1 DE69634696 D1 DE 69634696D1
Authority
DE
Germany
Prior art keywords
nucleic acid
test system
peptide nucleic
hybridization analysis
acid problems
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69634696T
Other languages
English (en)
Other versions
DE69634696T2 (de
Inventor
Martin Fuchs
Michael Egholm
Heather O'keefe
Xian W Yao
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems LLC
Original Assignee
PerSeptive Biosystems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by PerSeptive Biosystems Inc filed Critical PerSeptive Biosystems Inc
Application granted granted Critical
Publication of DE69634696D1 publication Critical patent/DE69634696D1/de
Publication of DE69634696T2 publication Critical patent/DE69634696T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE69634696T 1995-10-06 1996-10-04 Verfahren und testsystem zur hybridisierungsanalyse unter verwendung von peptidnukleinsäure-sonden Expired - Lifetime DE69634696T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US495395P 1995-10-06 1995-10-06
US4953 1995-10-06
PCT/US1996/015918 WO1997012995A1 (en) 1995-10-06 1996-10-04 Methods and kit for hybridization analysis using peptide nucleic acid probes

Publications (2)

Publication Number Publication Date
DE69634696D1 true DE69634696D1 (de) 2005-06-09
DE69634696T2 DE69634696T2 (de) 2006-01-19

Family

ID=21713369

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69634696T Expired - Lifetime DE69634696T2 (de) 1995-10-06 1996-10-04 Verfahren und testsystem zur hybridisierungsanalyse unter verwendung von peptidnukleinsäure-sonden

Country Status (5)

Country Link
US (2) US20020012902A1 (de)
EP (1) EP0854937B9 (de)
JP (1) JPH11512617A (de)
DE (1) DE69634696T2 (de)
WO (1) WO1997012995A1 (de)

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AU3829197A (en) * 1996-09-03 1998-03-26 Pioneer Hi-Bred International, Inc. Method and apparatus for single molecule two color fluorescent detection and molecular weight and concentration determination
DE69814848T2 (de) * 1997-02-27 2004-05-06 Ingeneus Corp. Untersuchung von nukleotiden in loesung mit hilfe von pna-sonden
US6251591B1 (en) * 1997-02-27 2001-06-26 Lorne Park Research, Inc. Quantitative method for detecting nucleotide concentration
US5846729A (en) * 1997-02-27 1998-12-08 Lorne Park Research, Inc. Assaying nucleotides in solution using a fluorescent intensity quenching effect
US6046004A (en) * 1997-02-27 2000-04-04 Lorne Park Research, Inc. Solution hybridization of nucleic acids with antisense probes having modified backbones
US6060242A (en) * 1997-02-27 2000-05-09 Lorne Park Research, Inc. PNA diagnostic methods
DE69834038D1 (de) 1997-07-01 2006-05-18 Isis Pharmaceutical Inc Zusammensetzungen und verfahren zur verabreichung von oligonukleotiden über die speiseröhre
US20030165953A1 (en) * 1997-07-09 2003-09-04 Isao Karube Method for detecting DNA with probe PNA
US6255050B1 (en) 1998-05-22 2001-07-03 Lorne Park Research, Inc. Dynamic hybridization system
EP1137807B1 (de) * 1998-12-08 2004-09-08 Boston Probes, Inc. Verfahren, reagentiensätze und zusammenstellungen zur erkennung von elektrostatisch an matrizen gebundene nukleinsäuren
US6441152B1 (en) 1998-12-08 2002-08-27 Boston Probes, Inc. Methods, kits and compositions for the identification of nucleic acids electrostatically bound to matrices
US6361671B1 (en) 1999-01-11 2002-03-26 The Regents Of The University Of California Microfabricated capillary electrophoresis chip and method for simultaneously detecting multiple redox labels
WO2000057170A1 (en) * 1999-03-19 2000-09-28 The Governors Of The University Of Alberta Automated 2-dimensional analysis of biological and other samples
US6645733B1 (en) 1999-06-25 2003-11-11 Ingeneus Corporation Fluorescent intensity method for assaying binding between proteins or peptides
US6312956B1 (en) 1999-10-01 2001-11-06 Vanderbilt University Nuclear targeted peptide nucleic acid oligomer
US7052844B2 (en) * 1999-12-21 2006-05-30 Ingeneus, Inc. Purification of DS-DNA using heteropolymeric capture probes and a triplex, quadruplex or homologous duplex binding mechanism
ES2263651T3 (es) 2000-08-30 2006-12-16 Avi Biopharma, Inc. Metodo para analidis de analogos de oligonucleotidos.
WO2002061410A1 (en) * 2000-09-01 2002-08-08 Spectrumedix Corporation System and method for temperature gradient capillary electrophoresis
AU2002214569A1 (en) * 2000-10-11 2002-04-22 Spectrumedix Corporation System and method for determining the presence of methylated cytosines in polynucleotides
WO2003027328A2 (en) 2001-09-24 2003-04-03 Boston Probes, Inc. Methods, kits and compositions pertaining to the suppression of detectable probe binding to randomly distributed repeat sequences in genomic nucleic acid
WO2003033743A1 (en) * 2001-10-18 2003-04-24 Spectrumedix Corporation System and method for temperature gradient capillary electrophoresis
US20030111494A1 (en) * 2001-10-26 2003-06-19 Sequenom, Inc. Method and apparatus for high-throughput sample handling process line
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
EP1487858A4 (de) * 2002-03-21 2006-01-04 Boston Probes Inc Pna-oligomere, oligomersätze, verfahren und kits zum nachweis von bacillus anthracis
US6872530B2 (en) * 2002-04-24 2005-03-29 Spectrumedix, Llc Method for determining the presence of DNA variants using peptide nucleic acid probes
US20040009473A1 (en) * 2002-06-07 2004-01-15 Christopher Pease Kit and process for microbiological for on-site examination of a liquid sample
US20040072217A1 (en) * 2002-06-17 2004-04-15 Affymetrix, Inc. Methods of analysis of linkage disequilibrium
US20040178070A1 (en) * 2002-07-16 2004-09-16 Zhaowei Liu Method and system for comparative genomics for organisms using temperature gradient electrophoresis
US7049073B2 (en) 2002-10-30 2006-05-23 The University Of Chicago Double stranded nucleic acid biochips
EP1629080B1 (de) * 2003-04-30 2017-07-05 William R. Moyle Verfahren zum biomolekularen nachweis und zur zellklassifizierung
US7303879B2 (en) * 2003-07-31 2007-12-04 Applera Corporation Determination of SNP allelic frequencies using temperature gradient electrophoresis
US7897747B2 (en) * 2006-05-25 2011-03-01 The Board Of Trustees Of The Leland Stanford Junior University Method to produce single stranded DNA of defined length and sequence and DNA probes produced thereby
EP1995328A1 (de) * 2007-05-22 2008-11-26 Koninklijke Philips Electronics N.V. Verfahren zur Erkennung einer Sonden-Target-Bindung
US20090215050A1 (en) * 2008-02-22 2009-08-27 Robert Delmar Jenison Systems and methods for point-of-care amplification and detection of polynucleotides
WO2010010858A1 (ja) * 2008-07-22 2010-01-28 アークレイ株式会社 キャピラリー電気泳動法による分析装置
CN102047104B (zh) * 2008-07-22 2014-09-03 爱科来株式会社 利用毛细管电泳法的分析装置及分析方法
EP2437887B1 (de) 2009-06-04 2016-05-11 Lockheed Martin Corporation Mikrofluidischer chip für dna analyse von mehrfachen proben
WO2012051529A1 (en) 2010-10-15 2012-04-19 Lockheed Martin Corporation Micro fluidic optic design
US9322054B2 (en) 2012-02-22 2016-04-26 Lockheed Martin Corporation Microfluidic cartridge
US10349589B2 (en) 2016-09-08 2019-07-16 Hemex Health, Inc. Diagnostics systems and methods
CN109923417B (zh) 2016-09-08 2021-06-08 希米斯保健股份有限公司 诊断***和方法
US10538808B2 (en) 2017-05-26 2020-01-21 Vibrant Holdings, Llc Photoactive compounds and methods for biomolecule detection and sequencing
US11988633B2 (en) * 2017-09-22 2024-05-21 Agilent Technologies, Inc. Use of polyamines as resolving agents for capillary electrophoresis of labeled glycans using gels
US11740203B2 (en) 2019-06-25 2023-08-29 Hemex Health, Inc. Diagnostics systems and methods
CN112326762B (zh) * 2020-10-29 2023-03-21 常州大学 一种毛细管电泳检测dna四面体的方法

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK51092D0 (da) * 1991-05-24 1992-04-15 Ole Buchardt Oligonucleotid-analoge betegnet pna, monomere synthoner og fremgangsmaade til fremstilling deraf samt anvendelser deraf
US6045995A (en) * 1994-08-24 2000-04-04 Isis Pharmaceuticals, Inc. Capillary electrophoretic detection of nucleic acids
US5582705A (en) * 1995-05-19 1996-12-10 Iowa State University Research Foundation, Inc. Multiplexed capillary electrophoresis system

Also Published As

Publication number Publication date
DE69634696T2 (de) 2006-01-19
EP0854937B9 (de) 2006-06-28
US20050053944A1 (en) 2005-03-10
EP0854937B1 (de) 2005-05-04
EP0854937A1 (de) 1998-07-29
US20020012902A1 (en) 2002-01-31
WO1997012995A1 (en) 1997-04-10
JPH11512617A (ja) 1999-11-02

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Legal Events

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Owner name: APPLIED BIOSYSTEMS, LLC (N. D. GES. D. STAATES, US