DE69331543T2 - Kovalent vernetzte oligonukleotide - Google Patents

Kovalent vernetzte oligonukleotide

Info

Publication number
DE69331543T2
DE69331543T2 DE69331543T DE69331543T DE69331543T2 DE 69331543 T2 DE69331543 T2 DE 69331543T2 DE 69331543 T DE69331543 T DE 69331543T DE 69331543 T DE69331543 T DE 69331543T DE 69331543 T2 DE69331543 T2 DE 69331543T2
Authority
DE
Germany
Prior art keywords
strand
oligonucleotides
cross
linkages
regions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69331543T
Other languages
English (en)
Other versions
DE69331543D1 (de
Inventor
Dan Cook
Muthian Manoharan
Thomas Bruice
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ionis Pharmaceuticals Inc
Original Assignee
Isis Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Isis Pharmaceuticals Inc filed Critical Isis Pharmaceuticals Inc
Application granted granted Critical
Publication of DE69331543D1 publication Critical patent/DE69331543D1/de
Publication of DE69331543T2 publication Critical patent/DE69331543T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
DE69331543T 1992-03-05 1993-03-05 Kovalent vernetzte oligonukleotide Expired - Lifetime DE69331543T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US84637692A 1992-03-05 1992-03-05
PCT/US1993/002059 WO1993018052A1 (en) 1992-03-05 1993-03-05 Covalently cross-linked oligonucleotides

Publications (2)

Publication Number Publication Date
DE69331543D1 DE69331543D1 (de) 2002-03-21
DE69331543T2 true DE69331543T2 (de) 2002-09-26

Family

ID=25297748

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69331543T Expired - Lifetime DE69331543T2 (de) 1992-03-05 1993-03-05 Kovalent vernetzte oligonukleotide

Country Status (8)

Country Link
US (1) US5719271A (de)
EP (1) EP0635023B1 (de)
JP (1) JP3530186B2 (de)
AT (1) ATE212998T1 (de)
CA (1) CA2131311C (de)
DE (1) DE69331543T2 (de)
IL (1) IL104965A (de)
WO (1) WO1993018052A1 (de)

Families Citing this family (63)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6087482A (en) * 1990-07-27 2000-07-11 Isis Pharmaceuticals, Inc. Heteroatomic oligonucleoside linkages
US5386023A (en) * 1990-07-27 1995-01-31 Isis Pharmaceuticals Backbone modified oligonucleotide analogs and preparation thereof through reductive coupling
US5998603A (en) 1994-09-29 1999-12-07 Isis Pharmaceuticals, Inc. 4'-desmethyl nucleoside analogs, and oligomers thereof
US5783682A (en) * 1990-07-27 1998-07-21 Isis Pharmaceuticals, Inc. Oligonucleotide mimics having nitrogen-containing linkages
US5602240A (en) * 1990-07-27 1997-02-11 Ciba Geigy Ag. Backbone modified oligonucleotide analogs
US5688941A (en) * 1990-07-27 1997-11-18 Isis Pharmaceuticals, Inc. Methods of making conjugated 4' desmethyl nucleoside analog compounds
US5608046A (en) * 1990-07-27 1997-03-04 Isis Pharmaceuticals, Inc. Conjugated 4'-desmethyl nucleoside analog compounds
US5618704A (en) * 1990-07-27 1997-04-08 Isis Pharmacueticals, Inc. Backbone-modified oligonucleotide analogs and preparation thereof through radical coupling
US5677437A (en) * 1990-07-27 1997-10-14 Isis Pharmaceuticals, Inc. Heteroatomic oligonucleoside linkages
US5610289A (en) * 1990-07-27 1997-03-11 Isis Pharmaceuticals, Inc. Backbone modified oligonucleotide analogues
US5792844A (en) * 1990-07-27 1998-08-11 Isis Pharmaceuticals, Inc. Oligonucleoside linkages containing adjacent nitrogen atoms
US5623070A (en) 1990-07-27 1997-04-22 Isis Pharmaceuticals, Inc. Heteroatomic oligonucleoside linkages
US5965722A (en) * 1991-05-21 1999-10-12 Isis Pharmaceuticals, Inc. Antisense inhibition of ras gene with chimeric and alternating oligonucleotides
US7223833B1 (en) 1991-05-24 2007-05-29 Isis Pharmaceuticals, Inc. Peptide nucleic acid conjugates
US6713602B1 (en) 1991-05-24 2004-03-30 Ole Buchardt Synthetic procedures for peptide nucleic acids
US6399376B1 (en) 1993-11-05 2002-06-04 Isis Pharmaceuticals, Inc. Modulation of vascular cell adhesive molecule expression through oligonucleotide interactions
AT407639B (de) * 1993-12-13 2001-05-25 Christian Dr Noe Modifizierte oligonukleotide, verfahren zu ihrer herstellung und verwendung als wirkstoffe zur herstellung von arzneimitteln
FR2732344B1 (fr) * 1995-04-03 1997-06-20 Genset Sa Oligonucleotides a liaison covalente transversale, procede de preparation et synthon utile dans le procede
US9096636B2 (en) * 1996-06-06 2015-08-04 Isis Pharmaceuticals, Inc. Chimeric oligomeric compounds and their use in gene modulation
US7812149B2 (en) * 1996-06-06 2010-10-12 Isis Pharmaceuticals, Inc. 2′-Fluoro substituted oligomeric compounds and compositions for use in gene modulations
US5898031A (en) * 1996-06-06 1999-04-27 Isis Pharmaceuticals, Inc. Oligoribonucleotides for cleaving RNA
US20040147022A1 (en) * 1996-06-06 2004-07-29 Baker Brenda F. 2'-methoxy substituted oligomeric compounds and compositions for use in gene modulations
US20040171031A1 (en) * 1996-06-06 2004-09-02 Baker Brenda F. Sugar surrogate-containing oligomeric compounds and compositions for use in gene modulation
US20050119470A1 (en) * 1996-06-06 2005-06-02 Muthiah Manoharan Conjugated oligomeric compounds and their use in gene modulation
US20050053976A1 (en) * 1996-06-06 2005-03-10 Baker Brenda F. Chimeric oligomeric compounds and their use in gene modulation
US20040203024A1 (en) * 1996-06-06 2004-10-14 Baker Brenda F. Modified oligonucleotides for use in RNA interference
US20040266706A1 (en) * 2002-11-05 2004-12-30 Muthiah Manoharan Cross-linked oligomeric compounds and their use in gene modulation
US20030165888A1 (en) * 2001-07-18 2003-09-04 Brown Bob D. Oligonucleotide probes and primers comprising universal bases for diagnostic purposes
US6518017B1 (en) * 1997-10-02 2003-02-11 Oasis Biosciences Incorporated Combinatorial antisense library
US6232463B1 (en) 1997-10-09 2001-05-15 Isis Pharmaceuticals, Inc. Substituted purines and oligonucleotide cross-linking
US6344330B1 (en) 1998-03-27 2002-02-05 The Regents Of The University Of California Pharmacophore recombination for the identification of small molecule drug lead compounds
US6277967B1 (en) 1998-07-14 2001-08-21 Isis Pharmaceuticals, Inc. Carbohydrate or 2′-modified oligonucleotides having alternating internucleoside linkages
US6492111B1 (en) * 1998-11-25 2002-12-10 Isis Pharmaceuticals, Inc. In situ binary synthesis of biologically effective molecules
DE19956568A1 (de) * 1999-01-30 2000-08-17 Roland Kreutzer Verfahren und Medikament zur Hemmung der Expression eines vorgegebenen Gens
AU2008202208C1 (en) * 1999-01-30 2014-04-24 Alnylam Pharmaceuticals, Inc. Method and medicament for inhibiting the expression of a defined gene
IL145586A0 (en) * 1999-04-08 2002-06-30 Oasis Biosciences Inc Antisense oligonucleotides comprising universal and/or degenerate bases
AU5103300A (en) * 1999-06-07 2000-12-28 Kenshi Obaru Primers for polymerase reactions
DE10100586C1 (de) * 2001-01-09 2002-04-11 Ribopharma Ag Verfahren zur Hemmung der Expression eines Ziegens
NZ553687A (en) 2000-03-30 2010-03-26 Whitehead Biomedical Inst RNA sequence-specific mediators of RNA interference
PT1407044E (pt) * 2000-12-01 2008-01-02 Max Planck Ges Zur Forderung W Moléculas curtas de arn que medeiam a interferência de arn
US7423142B2 (en) 2001-01-09 2008-09-09 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of anti-apoptotic genes
US7767802B2 (en) * 2001-01-09 2010-08-03 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of anti-apoptotic genes
US7176296B2 (en) * 2001-04-30 2007-02-13 Idera Pharmaceuticals, Inc. Modulation of oligonucleotide CpG-mediated immune stimulation by positional modification of nucleosides
US7105495B2 (en) * 2001-04-30 2006-09-12 Idera Pharmaceuticals, Inc. Modulation of oligonucleotide CpG-mediated immune stimulation by positional modification of nucleosides
CA2504929C (en) * 2002-11-05 2014-07-22 Charles Allerson Compositions comprising alternating 2'-modified nucleosides for use in gene modulation
EP1636342A4 (de) * 2003-06-20 2008-10-08 Isis Pharmaceuticals Inc Oligomere verbindungen zur verwendung bei der modulation von genen
US20050208538A1 (en) 2003-12-29 2005-09-22 Nurith Kurn Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
US8569474B2 (en) * 2004-03-09 2013-10-29 Isis Pharmaceuticals, Inc. Double stranded constructs comprising one or more short strands hybridized to a longer strand
WO2005121372A2 (en) * 2004-06-03 2005-12-22 Isis Pharmaceuticals, Inc. Double strand compositions comprising differentially modified strands for use in gene modulation
US8394947B2 (en) * 2004-06-03 2013-03-12 Isis Pharmaceuticals, Inc. Positionally modified siRNA constructs
US7884086B2 (en) * 2004-09-08 2011-02-08 Isis Pharmaceuticals, Inc. Conjugates for use in hepatocyte free uptake assays
WO2007102581A1 (ja) * 2006-03-08 2007-09-13 Tokyo Institute Of Technology 2'水酸基修飾リボヌクレオシド誘導体
US9206418B2 (en) 2011-10-19 2015-12-08 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing
GB2533882B (en) 2012-01-26 2016-10-12 Nugen Tech Inc Method of enriching and sequencing nucleic acids of interest using massively parallel sequencing
CN104619894B (zh) 2012-06-18 2017-06-06 纽亘技术公司 用于非期望核酸序列的阴性选择的组合物和方法
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
WO2014144092A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Sequential sequencing
EP3068883B1 (de) 2013-11-13 2020-04-29 Nugen Technologies, Inc. Zusammensetzungen und verfahren zur identifizierung einer duplikatsequenzierungslesung
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
WO2016022833A1 (en) 2014-08-06 2016-02-11 Nugen Technologies, Inc. Digital measurements from targeted sequencing
JP6491233B2 (ja) * 2014-12-12 2019-03-27 国立研究開発法人産業技術総合研究所 ハイブリダイゼーション安定化用核酸複合体、核酸ハイブリダイゼーションの安定化方法、アンチセンス核酸医薬品及びｍｉｃｒｏＲＮＡ抑制剤
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
US20230382947A1 (en) 2020-10-13 2023-11-30 Universiteit Gent Methods for proximity mediated coupling of a first agent to a second agent

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4123610A (en) * 1977-03-09 1978-10-31 The United States Government Nucleic acid crosslinking agent and affinity inactivation of nucleic acids therewith
US4868103A (en) * 1986-02-19 1989-09-19 Enzo Biochem, Inc. Analyte detection by means of energy transfer
US5082934A (en) * 1989-04-05 1992-01-21 Naxcor Coumarin derivatives for use as nucleotide crosslinking reagents
US4948494A (en) * 1989-06-28 1990-08-14 Union Oil Company Of California Removal of hydrogen sulfide from produced fluids
US5399676A (en) * 1989-10-23 1995-03-21 Gilead Sciences Oligonucleotides with inverted polarity
EP0523140A4 (en) * 1990-03-21 1993-06-16 Isis Pharmaceuticals, Inc. Reagents and methods for modulating gene expression through rna mimicry
US5223618A (en) * 1990-08-13 1993-06-29 Isis Pharmaceuticals, Inc. 4'-desmethyl nucleoside analog compounds
US5138045A (en) * 1990-07-27 1992-08-11 Isis Pharmaceuticals Polyamine conjugated oligonucleotides
US5378825A (en) * 1990-07-27 1995-01-03 Isis Pharmaceuticals, Inc. Backbone modified oligonucleotide analogs
EP0661979A4 (de) * 1991-08-21 1995-09-13 Microprobe Corp Vernetzen von oligonukleotiden für enzymvermittelte dreifachstrangbildung.
US5543507A (en) * 1992-03-05 1996-08-06 Isis Pharmaceuticals, Inc. Covalently cross-linked oligonucleotides

Also Published As

Publication number Publication date
ATE212998T1 (de) 2002-02-15
EP0635023B1 (de) 2002-02-06
WO1993018052A1 (en) 1993-09-16
IL104965A0 (en) 1993-07-08
CA2131311C (en) 2004-06-29
JP3530186B2 (ja) 2004-05-24
US5719271A (en) 1998-02-17
JPH07504438A (ja) 1995-05-18
EP0635023A1 (de) 1995-01-25
IL104965A (en) 1999-11-30
DE69331543D1 (de) 2002-03-21
EP0635023A4 (de) 1996-03-20
CA2131311A1 (en) 1993-08-16

Similar Documents

Publication Publication Date Title
DE69331543T2 (de) Kovalent vernetzte oligonukleotide
AU1933195A (en) Modified oligonucleotide duplexes having anticancer activity
ATE202569T1 (de) Prodrug-oligonukleotide
EP0251283A3 (de) Polymarkierte Oligonukleotid-Derivate
DE3886543D1 (de) Oligonukleotidfunktionierungsmittel und -verfahren.
ATE277068T1 (de) 2'-modifizierte oligonucleotide mit rns wirkung, die konformell eingeschränkt sind
CA2071536A1 (en) Triple helix formation in oligonucleotide therapy
DE69821867D1 (de) Gefriergetrocknete Produkte, Verfahren und Einrichtung zu ihrer Herstellung
EP1002877A3 (de) Methode zur Genvervielfältigung
EP0419654A4 (de) Wasserlösliches kationisches polymer.
BR9401355A (pt) Oligonucleotídeo e nucleotídeo.
DE3872920T2 (de) Beschichtete Produkte.
DE69308531D1 (de) Cycloisomaltooligosaccharide; Enzym und Verfahren zu ihrer Herstellung, und Verfahren zur Herstellung von dem Enzym
DE3851192D1 (de) Hologon und Verfahren zu seiner Herstellung.
AU6884596A (en) Systematic extraction, amplification and detection of retroviral sequences, and oligonucleotides for use therein
DE29508100U1 (de) Verteiler- bzw. Zustellerwagen
ITMI921235A1 (it) Oligonucleotidi antisenso ad attivita' antitumorale, composizioni farmaceutiche che li comprendono, e loro impieghi
DE69613971D1 (de) Oligonukelotide mit transversalkovalenter bindung, verfahren zu ihrer herstellung und darin benutztes synthon
ATE97913T1 (de) Annelierte azepinon- und azocinon-derivate, verfahren zu ihrer herstellung, diese enthaltende mittel und ihre verwendung, sowie zwischenprodukte bei ihrer herstellung.
CA2174816A1 (en) Tortillas Having Increased Flexibility and Process Therefore
DE69220201D1 (de) Antithrombotika, die sich von Cumarin ableiten, und Verfahren zu ihrer Herstellung
FR2748275B1 (fr) Derives de nucleosides, et leur utilisation pour la synthese d'oligonucleotides
DE3875845T2 (de) Neues hypoglykaemisches und wachstum anregendes mittel.
DE59800207D1 (de) Al2o3-haltiges, hochtextiles und hochtemperaturbeständiges glasstapelfaservorgarn sowie produkte hieraus
AU734526C (en) Nucleic acid probes and amplification oligonucleotides for neisseria species

Legal Events

Date Code Title Description
8364 No opposition during term of opposition