DE60322214D1 - "frog-prince" - ein transposonvektor für den gentransfer bei wirbeltieren - Google Patents

"frog-prince" - ein transposonvektor für den gentransfer bei wirbeltieren

Info

Publication number
DE60322214D1
DE60322214D1 DE60322214T DE60322214T DE60322214D1 DE 60322214 D1 DE60322214 D1 DE 60322214D1 DE 60322214 T DE60322214 T DE 60322214T DE 60322214 T DE60322214 T DE 60322214T DE 60322214 D1 DE60322214 D1 DE 60322214D1
Authority
DE
Germany
Prior art keywords
transposon
selectable marker
integration system
gene
based dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60322214T
Other languages
English (en)
Inventor
Csaba Miskey
Zsuzsanna Izsvak
Zoltan Ivics
Ronald Plasterk
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
NETHERLAND CANCER INST
Max Delbrueck Centrum fuer Molekulare in der Helmholtz Gemeinschaft
Original Assignee
NETHERLAND CANCER INST
Max Delbrueck Centrum fuer Molekulare in der Helmholtz Gemeinschaft
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by NETHERLAND CANCER INST, Max Delbrueck Centrum fuer Molekulare in der Helmholtz Gemeinschaft filed Critical NETHERLAND CANCER INST
Application granted granted Critical
Publication of DE60322214D1 publication Critical patent/DE60322214D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/60—Vectors containing traps for, e.g. exons, promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/90—Vectors containing a transposable element
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE60322214T 2002-05-29 2003-05-30 "frog-prince" - ein transposonvektor für den gentransfer bei wirbeltieren Expired - Lifetime DE60322214D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10224242A DE10224242A1 (de) 2002-05-29 2002-05-29 Frog Prince, ein Transposonvektor für den Gentransfer bei Wirbeltieren
PCT/EP2003/005707 WO2003100070A2 (en) 2002-05-29 2003-05-30 The frog prince, a transposon vector for gene transfer in vertebrates

Publications (1)

Publication Number Publication Date
DE60322214D1 true DE60322214D1 (de) 2008-08-28

Family

ID=29432501

Family Applications (2)

Application Number Title Priority Date Filing Date
DE10224242A Withdrawn DE10224242A1 (de) 2002-05-29 2002-05-29 Frog Prince, ein Transposonvektor für den Gentransfer bei Wirbeltieren
DE60322214T Expired - Lifetime DE60322214D1 (de) 2002-05-29 2003-05-30 "frog-prince" - ein transposonvektor für den gentransfer bei wirbeltieren

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE10224242A Withdrawn DE10224242A1 (de) 2002-05-29 2002-05-29 Frog Prince, ein Transposonvektor für den Gentransfer bei Wirbeltieren

Country Status (8)

Country Link
US (1) US20050241007A1 (de)
EP (1) EP1507865B1 (de)
JP (1) JP4493492B2 (de)
AT (1) ATE401411T1 (de)
AU (1) AU2003238444A1 (de)
DE (2) DE10224242A1 (de)
DK (1) DK1507865T3 (de)
WO (1) WO2003100070A2 (de)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5320546B2 (ja) 2006-12-13 2013-10-23 国立大学法人名古屋大学 Ｔｏｌ１因子のトランスポザーゼ及びそれを用いたＤＮＡ導入システム
HU0700675D0 (en) 2007-10-15 2007-12-28 Mta Tamogatott Kutatohelyek Ir Method for monitoring stem cell differentiation
EP2067402A1 (de) 2007-12-07 2009-06-10 Max Delbrück Centrum für Molekulare Medizin (MDC) Berlin-Buch; Transposon-vermittelte Mutagenese in Spermatogoniestammzellen
WO2010008564A2 (en) * 2008-07-16 2010-01-21 Recombinetics Plaice dna transposon system
WO2010008562A2 (en) 2008-07-16 2010-01-21 Recombinetics Methods and materials for producing transgenic animals
EP2539450B1 (de) * 2010-02-25 2016-02-17 Advanced Liquid Logic, Inc. Verfahren zur herstellung von nukleinsäure-bibliotheken
US20110312001A1 (en) 2010-06-15 2011-12-22 Emile Nuwaysir Compendium of ready-built stem cell models for interrogation of biological response
EP2591093B1 (de) 2010-07-07 2018-11-28 FUJIFILM Cellular Dynamics, Inc. Endothelzellenproduktion durch programmierung
WO2012018933A2 (en) 2010-08-04 2012-02-09 Cellular Dynamics International, Inc. Reprogramming immortalized b cells
WO2012109208A2 (en) 2011-02-08 2012-08-16 Cellular Dynamics International, Inc. Hematopoietic precursor cell production by programming
CA2841165A1 (en) 2011-07-11 2013-01-17 Cellular Dynamics International, Inc. Methods for cell reprogramming and genome engineering
CN105229144A (zh) 2013-02-22 2016-01-06 细胞动力学国际有限公司 通过组合的遗传工程和化学工程经由正向编程产生肝细胞
WO2014132137A2 (en) 2013-03-01 2014-09-04 Université De Genève Transgenic cell selection
US20170107486A1 (en) 2014-04-21 2017-04-20 Cellular Dynamics International, Inc. Hepatocyte production via forward programming by combined genetic and chemical engineering
AU2016342179B2 (en) 2015-10-20 2022-08-18 FUJIFILM Cellular Dynamics, Inc. Multi-lineage hematopoietic precursor cell production by genetic programming
GB201518910D0 (en) 2015-10-26 2015-12-09 Rieke Packaging Systems Ltd Dispensers
WO2017096363A1 (en) 2015-12-04 2017-06-08 Board Of Regents, The University Of Texas System Reporter system for detecting and targeting activated cells
IL265810B1 (en) 2016-10-05 2024-04-01 Fujifilm Cellular Dynamics Inc A method for the direct separation of pluripotent stem cells into homozygous HLA cells of the immune system
CA3038690A1 (en) 2016-10-05 2018-04-12 FUJIFILM Cellular Dynamics, Inc. Generating mature lineages from induced pluripotent stem cells with mecp2 disruption
EP4083063A3 (de) 2017-04-18 2023-01-04 FUJIFILM Cellular Dynamics, Inc. Antigenspezifische immuneffektorzellen
US11649455B2 (en) 2018-03-30 2023-05-16 University Of Geneva Micro RNA expression constructs and uses thereof
BR112021026832A2 (pt) 2019-07-02 2022-05-10 Hutchinson Fred Cancer Res Vetores ad35 recombinantes e aprimoramentos da terapia gênica relacionada
EP4157864A1 (de) 2020-05-27 2023-04-05 Antion Biosciences SA Adaptermoleküle zur umleitung von car-t-zellen zu einem antigen von interesse
JP2024519218A (ja) 2021-05-03 2024-05-09 アステラス インスティテュート フォー リジェネレイティブ メディシン 成熟角膜内皮細胞を作製する方法
AU2022270117A1 (en) 2021-05-07 2023-11-02 Astellas Institute For Regenerative Medicine Methods of generating mature hepatocytes
GB202206507D0 (en) 2022-05-04 2022-06-15 Antion Biosciences Sa Expression construct

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69841648D1 (de) * 1997-03-11 2010-06-17 Univ Minnesota Dns-basiertes transposon-system für die einführung von nucleinsäure in die dns einer zelle
WO1999025817A2 (en) * 1997-11-13 1999-05-27 Regents Of The University Of Minnesota Tc1-based transposon vectors

Also Published As

Publication number Publication date
AU2003238444A8 (en) 2003-12-12
DK1507865T3 (da) 2008-10-13
EP1507865B1 (de) 2008-07-16
EP1507865A2 (de) 2005-02-23
ATE401411T1 (de) 2008-08-15
JP4493492B2 (ja) 2010-06-30
WO2003100070A3 (en) 2004-12-09
WO2003100070A2 (en) 2003-12-04
AU2003238444A1 (en) 2003-12-12
DE10224242A1 (de) 2003-12-11
JP2005527216A (ja) 2005-09-15
US20050241007A1 (en) 2005-10-27

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Representative=s name: GRAF VON STOSCH PATENTANWALTSGESELLSCHAFT MBH, 805