DE60228952D1 - Nachweis von Legionella durch ein PCR und FRET verwendendes Verfahren - Google Patents

Nachweis von Legionella durch ein PCR und FRET verwendendes Verfahren

Info

Publication number
DE60228952D1
DE60228952D1 DE60228952T DE60228952T DE60228952D1 DE 60228952 D1 DE60228952 D1 DE 60228952D1 DE 60228952 T DE60228952 T DE 60228952T DE 60228952 T DE60228952 T DE 60228952T DE 60228952 D1 DE60228952 D1 DE 60228952D1
Authority
DE
Germany
Prior art keywords
legionella
detection
pcr
fret
pneumophila
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60228952T
Other languages
English (en)
Inventor
Franklin R Cockerill Iii
Randall T Hayden
James R Uhl
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mayo Foundation for Medical Education and Research
Original Assignee
Mayo Foundation for Medical Education and Research
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mayo Foundation for Medical Education and Research filed Critical Mayo Foundation for Medical Education and Research
Application granted granted Critical
Publication of DE60228952D1 publication Critical patent/DE60228952D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Diaphragms For Electromechanical Transducers (AREA)
  • Ropes Or Cables (AREA)
DE60228952T 2001-05-07 2002-05-07 Nachweis von Legionella durch ein PCR und FRET verwendendes Verfahren Expired - Lifetime DE60228952D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US28922201P 2001-05-07 2001-05-07
PCT/US2002/014313 WO2002089844A1 (en) 2001-05-07 2002-05-07 Legionella detection with fluorescent resonance energy transfer

Publications (1)

Publication Number Publication Date
DE60228952D1 true DE60228952D1 (de) 2008-10-30

Family

ID=23110577

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60228952T Expired - Lifetime DE60228952D1 (de) 2001-05-07 2002-05-07 Nachweis von Legionella durch ein PCR und FRET verwendendes Verfahren

Country Status (5)

Country Link
US (1) US6830888B2 (de)
EP (1) EP1392362B1 (de)
AT (1) ATE408710T1 (de)
DE (1) DE60228952D1 (de)
WO (1) WO2002089844A1 (de)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7615780B2 (en) * 2000-10-23 2009-11-10 General Electric Company DNA biosensor and methods for making and using the same
US7297484B2 (en) * 2002-04-26 2007-11-20 Idaho Technology Characterization of single stranded nucleic acids by melting analysis of secondary structure using double strand-specific nucleic acid dye
US7282331B2 (en) * 2003-03-18 2007-10-16 Advandx, Inc. Method for improved specificity in probe based assays
KR100681837B1 (ko) * 2005-06-09 2007-02-12 건국대학교 산학협력단 리얼타임 ｐｃｒ을 이용한 리케챠 및 스크럽 티푸스그룹（ｓｔｇ）균의 검출방법
JP2009511086A (ja) * 2005-10-17 2009-03-19 ジェン−プローブ・インコーポレーテッド レジオネラ・ニューモフィラ核酸を検出するための組成物及び方法
US8609829B2 (en) * 2005-10-17 2013-12-17 Gen-Probe Incorporated Compositions and methods to detect Legionella pneumophila nucleic acid
ATE531818T1 (de) * 2006-05-02 2011-11-15 Univ Paris Curie Methode zur bestimmung und auszählung von mikroorganismen
US8535888B2 (en) * 2006-12-29 2013-09-17 Mayo Foundation For Medical Education And Research Compositions and methods for detecting methicillin-resistant S. aureus
CN102105602A (zh) * 2008-07-30 2011-06-22 通用电气公司 评价致病两期细菌的毒力的方法
EP3150723B1 (de) 2010-06-21 2019-01-30 Life Technologies Corporation Zusammensetzungen, kits und verfahren zur synthese und/oder zum nachweis von nukleinsäuren
US10738327B2 (en) * 2017-08-28 2020-08-11 Inscripta, Inc. Electroporation cuvettes for automation

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4851333A (en) * 1984-02-24 1989-07-25 Genetic Systems Corporation Method and composition for the detection and diagnosis of Legionella pneumophila
US4722891A (en) 1984-06-20 1988-02-02 Board Of Regents, The University Of Texas System Methods and compositions for detection of Legionnaires' disease
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4996143A (en) 1985-12-23 1991-02-26 Syngene, Inc. Fluorescent stokes shift probes for polynucleotide hybridization
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5994059A (en) 1986-11-24 1999-11-30 Gen-Probe Incorporated Nucleic acid probes and methods for detecting Streptomyces enterococci
US5541308A (en) 1986-11-24 1996-07-30 Gen-Probe Incorporated Nucleic acid probes for detection and/or quantitation of non-viral organisms
US5035996A (en) 1989-06-01 1991-07-30 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
US5683896A (en) 1989-06-01 1997-11-04 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
US5491225A (en) 1991-12-19 1996-02-13 Hoffmann-La Roche Inc. PCR primers for detection of legionella species and methods for controlling visual intensity in hybridization assays
JP3509859B2 (ja) 1991-11-07 2004-03-22 ナノトロニクス，インコーポレイテッド 供与体−供与体エネルギー転移系を創製するための発色団および蛍光団でコンジュゲート化されたポリヌクレオチドのハイブリダイゼーション
EP0656070A4 (de) 1993-05-24 1996-03-20 Amoco Corp Nukleinsäureproben für bakterien der gattung legionella.
ATE428801T1 (de) 1996-06-04 2009-05-15 Univ Utah Res Found Überwachung der hybridisierung während pcr
DE69738605T2 (de) 1996-06-04 2009-04-30 University Of Utah Research Foundation, Salt Lake City Behälter zum Durchführen und Beobachten biologischer Prozesse
US5968739A (en) * 1996-12-10 1999-10-19 Abbott Laboratories Nucleic acid primers and probes for detecting Legionella pneumophila
CA2286310A1 (en) 1997-04-22 1998-10-29 Bavarian Nordic Research Institute A/S Taqmantm-pcr for the detection of pathogenic e. coli strains
GB9803382D0 (en) * 1998-02-19 1998-04-15 Secr Defence Detection system
JP2000184894A (ja) * 1998-10-15 2000-07-04 Japan Bioindustry Association 一対の核酸プロ―ブおよびそのプロ―ブを用いる複合微生物系または共生微生物系における特定グル―プ若しくは特定菌の存在量を測定する方法
US6162605A (en) 1999-04-12 2000-12-19 Becton Dickinson And Company Amplification and detection of shiga-like toxin I producing organisms
US6387621B1 (en) * 1999-04-27 2002-05-14 University Of Utah Research Foundation Automated analysis of real-time nucleic acid amplification
US6251609B1 (en) * 2000-07-27 2001-06-26 Becton, Dickinson And Company Amplification and detection of Legionella pneumophila targeting the mip gene

Also Published As

Publication number Publication date
EP1392362A4 (de) 2006-08-30
EP1392362B1 (de) 2008-09-17
WO2002089844A1 (en) 2002-11-14
US6830888B2 (en) 2004-12-14
US20030082577A1 (en) 2003-05-01
EP1392362A1 (de) 2004-03-03
ATE408710T1 (de) 2008-10-15

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