DE602005023705D1 - Echtzeit-pcr von zielen auf einem mikroarray - Google Patents

Echtzeit-pcr von zielen auf einem mikroarray

Info

Publication number
DE602005023705D1
DE602005023705D1 DE602005023705T DE602005023705T DE602005023705D1 DE 602005023705 D1 DE602005023705 D1 DE 602005023705D1 DE 602005023705 T DE602005023705 T DE 602005023705T DE 602005023705 T DE602005023705 T DE 602005023705T DE 602005023705 D1 DE602005023705 D1 DE 602005023705D1
Authority
DE
Germany
Prior art keywords
nucleotide molecule
target nucleotide
molecule
solution
labelled target
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602005023705T
Other languages
English (en)
Inventor
Jose Remacle
Isabelle Alexandre
Sylvain Margaine
Dieter Husar
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf Array Technologies SA
Original Assignee
Eppendorf Array Technologies SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=35539286&utm_source=***_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE602005023705(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from EP04027435A external-priority patent/EP1659183A1/de
Priority claimed from US10/991,087 external-priority patent/US8288128B2/en
Application filed by Eppendorf Array Technologies SA filed Critical Eppendorf Array Technologies SA
Publication of DE602005023705D1 publication Critical patent/DE602005023705D1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0822—Slides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Physics & Mathematics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Clinical Laboratory Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Optics & Photonics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Radiation-Therapy Devices (AREA)
  • Radio Relay Systems (AREA)
DE602005023705T 2004-11-18 2005-11-18 Echtzeit-pcr von zielen auf einem mikroarray Active DE602005023705D1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP04027435A EP1659183A1 (de) 2004-11-18 2004-11-18 Quantifizierung einer Vielzahl von Zielmolekülen auf einem Mikroarray in real-time
US10/991,087 US8288128B2 (en) 2004-11-18 2004-11-18 Real-time quantification of multiple targets on a micro-array
PCT/EP2005/012383 WO2006053770A1 (en) 2004-11-18 2005-11-18 Real-time pcr of targets on a micro-array

Publications (1)

Publication Number Publication Date
DE602005023705D1 true DE602005023705D1 (de) 2010-11-04

Family

ID=35539286

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602005023705T Active DE602005023705D1 (de) 2004-11-18 2005-11-18 Echtzeit-pcr von zielen auf einem mikroarray

Country Status (6)

Country Link
US (1) US20090191618A1 (de)
EP (3) EP2302072B1 (de)
JP (1) JP5026978B2 (de)
AT (3) ATE482288T1 (de)
DE (1) DE602005023705D1 (de)
WO (2) WO2006053770A1 (de)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7829313B2 (en) 2000-03-24 2010-11-09 Eppendorf Array Technologies Identification and quantification of a plurality of biological (micro)organisms or their components
US7875442B2 (en) 2000-03-24 2011-01-25 Eppendorf Array Technologies Identification and quantification of a plurality of biological (micro)organisms or their components
CA2348042A1 (en) 2001-06-04 2002-12-04 Ann Huletsky Sequences for detection and identification of methicillin-resistant staphylococcus aureus
US11834720B2 (en) 2005-10-11 2023-12-05 Geneohm Sciences, Inc. Sequences for detection and identification of methicillin-resistant Staphylococcus aureus (MRSA) of MREJ types xi to xx
EP2041301B1 (de) * 2006-05-17 2014-12-31 Eppendorf Array Technologies SA Identifizierung und quantifizierung mehrerer biologischer (mikro-)organismen oder deren komponenten
US9133504B2 (en) 2006-06-05 2015-09-15 California Institute Of Technology Real time microarrays
US8637436B2 (en) 2006-08-24 2014-01-28 California Institute Of Technology Integrated semiconductor bioarray
US11001881B2 (en) 2006-08-24 2021-05-11 California Institute Of Technology Methods for detecting analytes
US8048626B2 (en) 2006-07-28 2011-11-01 California Institute Of Technology Multiplex Q-PCR arrays
US11525156B2 (en) 2006-07-28 2022-12-13 California Institute Of Technology Multiplex Q-PCR arrays
US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
JP2008102117A (ja) * 2006-09-21 2008-05-01 Fujifilm Corp 表面プラズモン増強蛍光センサおよび蛍光検出方法
EP1946841A1 (de) * 2006-12-22 2008-07-23 Eppendorf Array Technologies SA Vorrichtung und/oder Verfahren zum Nachweis von amplifizierten Nukleotidsequenzen auf Mikroarrays
EP1935496A1 (de) * 2006-12-22 2008-06-25 Eppendorf Array Technologies SA Vorrichtung und/oder Verfahren zur Detektion erweiterter Nukleotidsequenzen auf Mikro-Arrays
JP2009060859A (ja) * 2007-09-07 2009-03-26 Sumitomo Bakelite Co Ltd 遺伝子の検出方法
JP2011508219A (ja) * 2007-12-19 2011-03-10 シンギュレックス・インコーポレイテッド 単一分子検出走査分析器およびその使用方法
EP2245467B1 (de) 2008-02-05 2022-04-06 Pocared Diagnostics Ltd. System zur durchführung der identifizierung von bakterien in biologischen proben
EP2107125A1 (de) 2008-03-31 2009-10-07 Eppendorf Array Technologies SA (EAT) Echtzeit-PCR von Targets auf einem Mikroarray
US20100069253A1 (en) * 2008-09-12 2010-03-18 Gindilis Andrei L Impedance Spectroscopy Measurement of DNA
EP2382324A1 (de) 2008-12-30 2011-11-02 STMicroelectronics S.r.l. Verfahren, mikroreaktor und vorrichtung zur durchführung von nukleinsäureamplifikation in echtzeit
WO2010084381A1 (en) * 2009-01-21 2010-07-29 Eppendorf Ag Detection and/or quantification method of target molecules on a solid support
EP2221605A1 (de) * 2009-02-12 2010-08-25 Koninklijke Philips Electronics N.V. Drahtgittersensor
US10288632B2 (en) 2009-09-21 2019-05-14 Pocared Diagnostics Ltd. System for conducting the identification of bacteria in biological samples
EP2369325A1 (de) 2010-03-12 2011-09-28 Eppendorf Ag Arrayanalyse für Online-Detektion
EP2441520A1 (de) 2010-10-12 2012-04-18 Eppendorf AG Echtzeitverstärkungs- und Mikroarray-basierte Erkennung von Nukleinsäuretargets in einem Fluss-Chip-Assay
AU2011342975B2 (en) 2010-12-17 2015-04-23 Bjs Ip Limited Methods and systems for fast PCR heating
WO2013080432A1 (ja) * 2011-11-28 2013-06-06 ソニー株式会社 ケミカルセンサ、ケミカルセンサモジュール、化学物質検出装置及び化学物質検出方法
WO2013138685A1 (en) * 2012-03-16 2013-09-19 Life Technologies Corporation Systems and methods for assessing of biological samples
EP2855019A1 (de) 2012-05-24 2015-04-08 BJS IP Limited Klemme für schnelles pcr-erwärmen
US9708647B2 (en) 2015-03-23 2017-07-18 Insilixa, Inc. Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays
US9499861B1 (en) 2015-09-10 2016-11-22 Insilixa, Inc. Methods and systems for multiplex quantitative nucleic acid amplification
KR20170099738A (ko) 2016-02-23 2017-09-01 노을 주식회사 접촉식 염색 패치 및 그 제조 방법
US10371610B2 (en) 2016-02-23 2019-08-06 Noul Co., Ltd. Contact-type patch, staining method using the same, and manufacturing method thereof
KR102478636B1 (ko) * 2016-02-23 2022-12-19 노을 주식회사 중합 효소 연쇄 반응 패치, 이를 이용하는 진단 방법 및 장치
KR20170099787A (ko) * 2016-02-23 2017-09-01 노을 주식회사 표지 물질을 저장하는 패치, 이를 이용하는 조직 진단 방법 및 장치
WO2017155858A1 (en) 2016-03-07 2017-09-14 Insilixa, Inc. Nucleic acid sequence identification using solid-phase cyclic single base extension
JP2022525322A (ja) 2019-03-14 2022-05-12 インシリクサ， インコーポレイテッド 時間ゲート蛍光ベースの検出のための方法およびシステム
CN116515617B (zh) * 2023-07-03 2023-10-20 杭州杰毅生物技术有限公司 一种离心式pcr-荧光多靶标检测芯片

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL154598B (nl) 1970-11-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking.
US3817837A (en) 1971-05-14 1974-06-18 Syva Corp Enzyme amplification assay
US3939350A (en) 1974-04-29 1976-02-17 Board Of Trustees Of The Leland Stanford Junior University Fluorescent immunoassay employing total reflection for activation
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4277437A (en) 1978-04-05 1981-07-07 Syva Company Kit for carrying out chemically induced fluorescence immunoassay
US4275149A (en) 1978-11-24 1981-06-23 Syva Company Macromolecular environment control in specific receptor assays
US4366241A (en) 1980-08-07 1982-12-28 Syva Company Concentrating zone method in heterogeneous immunoassays
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
JP2909216B2 (ja) 1994-04-29 1999-06-23 パーキン‐エルマー コーポレイション 核酸増幅生成物のリアルタイム検出装置
WO1996034983A1 (en) 1995-05-05 1996-11-07 The Perkin-Elmer Corporation Methods and reagents for combined pcr amplification and hybridization probing assay
JP2002515738A (ja) 1996-01-23 2002-05-28 アフィメトリックス，インコーポレイティド 核酸分析法
US5716784A (en) 1996-02-05 1998-02-10 The Perkin-Elmer Corporation Fluorescence detection assay for homogeneous PCR hybridization systems
AU5716098A (en) * 1996-12-23 1998-07-17 University Of Chicago, The Customized oligonucleotide microchips as multiple biosensors
US6333155B1 (en) 1997-12-19 2001-12-25 Affymetrix, Inc. Exploiting genomics in the search for new drugs
US5952202A (en) 1998-03-26 1999-09-14 The Perkin Elmer Corporation Methods using exogenous, internal controls and analogue blocks during nucleic acid amplification
US20020177135A1 (en) * 1999-07-27 2002-11-28 Doung Hau H. Devices and methods for biochip multiplexing
DE19949735A1 (de) 1999-10-15 2001-05-10 Bruker Daltonik Gmbh Prozessieren von Proben in Lösungen mit definiert kleiner Wandkontaktfläche
US6316200B1 (en) * 2000-06-08 2001-11-13 Becton, Dickinson And Company Probes and methods for detection of nucleic acids
AU6834700A (en) * 1999-08-13 2001-03-13 Zeptosens Ag Device and method for determining multiple analytes
US7205104B2 (en) 2000-03-24 2007-04-17 Eppendorf Array Technologies Sa (Eat) Identification of biological (micro) organisms by detection of their homologous nucleotide sequences on arrays
JP3963422B2 (ja) * 2000-08-03 2007-08-22 日鉄環境エンジニアリング株式会社 核酸の測定方法
EP1184349A1 (de) 2000-09-01 2002-03-06 A.S.B.L. Facultes Universitaires Notre-Dame De La Paix Verfahren zur Erhaltung einer Oberflächenaktivierung eines festen Trägers zur Bildung von Biochips Mikroanordnungen
US6754414B2 (en) * 2001-09-27 2004-06-22 Bio-Rad Laboratories, Inc. Imaging of microarrays using fiber optic exciter
MXPA04006861A (es) * 2002-01-18 2005-06-20 Univ Utah Res Found Deteccion de polimorfismos de nucleotidos individuales utilizando guias de onda planas.
US7935500B2 (en) * 2002-03-26 2011-05-03 Massachusetts Institute Of Technology Identifying calcineurin activators for treatment of schizophrenia
DE10315074A1 (de) * 2003-04-02 2004-10-14 Clondiag Chip Technologies Gmbh Vorrichtung zur Vervielfältigung und zum Nachweis von Nukleinsäuren
JP4074829B2 (ja) * 2003-04-16 2008-04-16 大日本印刷株式会社 バイオマイクロアレイ用基板およびバイオマイクロアレイ
US20060030036A1 (en) * 2004-05-28 2006-02-09 Victor Joseph Chips for multiplex analyses
US20060088844A1 (en) * 2004-10-22 2006-04-27 Honeywell International Inc. Real-time PCR microarray based on evanescent wave biosensor

Also Published As

Publication number Publication date
JP2009530589A (ja) 2009-08-27
EP1812594A1 (de) 2007-08-01
JP5026978B2 (ja) 2012-09-19
EP2302072B1 (de) 2012-03-14
WO2006053770A8 (en) 2006-08-24
US20090191618A1 (en) 2009-07-30
EP1704258A1 (de) 2006-09-27
ATE505560T1 (de) 2011-04-15
WO2006053770A1 (en) 2006-05-26
ATE549420T1 (de) 2012-03-15
ATE482288T1 (de) 2010-10-15
EP1812594B1 (de) 2013-01-09
WO2006053769A1 (en) 2006-05-26
EP1704258B1 (de) 2010-09-22
EP2302072A1 (de) 2011-03-30

Similar Documents

Publication Publication Date Title
ATE482288T1 (de) Echtzeit-pcr von zielen auf einem mikroarray
US8247196B2 (en) Real-time PCR of targets on a micro-array
WO2006135437A3 (en) Real-time pcr microarray based on an evanescent wave biosensor
JP2006523095A5 (de)
WO2004087951A3 (de) Vorrichtung zur vervielfältigung und zum nachweis von nukleinsäuren
RU2008148143A (ru) Микроэлектронное сенсорное устройство для детектирования днк
Peter et al. Optical DNA-sensor chip for real-time detection of hybridization events
WO2005108609A1 (en) Method for identification and analysis of certain molecules using the dual function of single strand nucleic acid
ATE398186T1 (de) Integriertes verfahren und system zur amplifizierung und zum nachweis von nukleinsäuren
RU2582250C2 (ru) Способ тестирования и контроль качества нуклеиновых кислот на подложке
US8288128B2 (en) Real-time quantification of multiple targets on a micro-array
WO2011009893A1 (en) A membrane based microarray device for high-throughput multianalyte detection
US20080199861A1 (en) Real-time microarray apparatus and methods related thereto
Yang et al. A novel strategy for analyzing RNA-protein interactions by surface plasmon resonance biosensor
Patel A review on plant disease diagnosis through biosensor
ATE541050T1 (de) Analyse von biologischen zielmolekülen unter verwendung eines einen fluoreszenzmarker tragenden biochips
JP4538746B2 (ja) バイオチップおよび分析装置
KR101017199B1 (ko) 핵산 마이크로어레이를 이용한 핵산 검출 방법
EP3287768B1 (de) Erkennung von unterschiedlichen molekülen mit glasfasersensoren
JP3705499B2 (ja) ターゲット核酸断片の分析方法及びターゲット核酸断片の分析キット
EP2119797A4 (de) Verfahren zum nachweis von amplifikation oder deletion in einem genomischen dna-fragment
US20080164424A1 (en) Reducing Background Fluorescence in Arrays
JP4079808B2 (ja) 核酸増幅およびハイブリダイゼーション検出が可能なプローブ固相化反応アレイ
WO2024028886A1 (en) A miniature quantitative polymerase chain reaction apparatus
JP2006029953A (ja) 生体関連物質検出用固相化担体及びプローブの固相化方法、並びに生体関連物質解析方法