DE602005016707D1 - Stabile genomische integration mehrerer polynukleotidkopien - Google Patents

Stabile genomische integration mehrerer polynukleotidkopien

Info

Publication number
DE602005016707D1
DE602005016707D1 DE602005016707T DE602005016707T DE602005016707D1 DE 602005016707 D1 DE602005016707 D1 DE 602005016707D1 DE 602005016707 T DE602005016707 T DE 602005016707T DE 602005016707 T DE602005016707 T DE 602005016707T DE 602005016707 D1 DE602005016707 D1 DE 602005016707D1
Authority
DE
Germany
Prior art keywords
genome integration
multiple polynucleotide
polynucleotide copies
stable genome
copies
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602005016707T
Other languages
English (en)
Inventor
Anne Breuener
Michael Dolberg Rasmussen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novozymes AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes AS filed Critical Novozymes AS
Publication of DE602005016707D1 publication Critical patent/DE602005016707D1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/50—Vector systems having a special element relevant for transcription regulating RNA stability, not being an intron, e.g. poly A signal

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Electronic Switches (AREA)
DE602005016707T 2004-10-22 2005-10-19 Stabile genomische integration mehrerer polynukleotidkopien Active DE602005016707D1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA200401621 2004-10-22
DKPA200401785 2004-11-17
PCT/DK2005/000673 WO2006042548A1 (en) 2004-10-22 2005-10-19 Stable genomic integration of multiple polynucleotide copies

Publications (1)

Publication Number Publication Date
DE602005016707D1 true DE602005016707D1 (de) 2009-10-29

Family

ID=35596262

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602005016707T Active DE602005016707D1 (de) 2004-10-22 2005-10-19 Stabile genomische integration mehrerer polynukleotidkopien

Country Status (7)

Country Link
US (3) US20080085535A1 (de)
EP (1) EP1805296B1 (de)
CN (1) CN101061214B (de)
AT (1) ATE443128T1 (de)
DE (1) DE602005016707D1 (de)
DK (1) DK1805296T3 (de)
WO (1) WO2006042548A1 (de)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005123915A1 (en) 2004-06-21 2005-12-29 Novozymes A/S Stably maintained multiple copies of at least two orf in the same orientation
EP1805296B1 (de) * 2004-10-22 2009-09-16 Novozymes A/S Stabile genomische integration mehrerer polynukleotidkopien
WO2008142028A1 (en) * 2007-05-17 2008-11-27 Boehringer Ingelheim Rcv Gmbh & Co Kg Method for producing a recombinant protein on a manufacturing scale
US9109012B2 (en) * 2007-05-29 2015-08-18 Nature Technology Corporation Vectors and method for genetic immunization
US20110236927A1 (en) * 2008-08-27 2011-09-29 Massachusetts Institute Of Technology Genetically stabilized tandem gene duplication
EP2527448A1 (de) 2011-05-23 2012-11-28 Novozymes A/S Gleichzeitige standortspezifische Integrationen von mehreren Genkopien in faserige Pilze
CN104981542B (zh) 2012-03-12 2018-10-16 帝斯曼知识产权资产管理有限公司 重组***
US9631197B2 (en) * 2012-03-12 2017-04-25 Dsm Ip Assets B.V. Rasamsonia transformants
US9657309B2 (en) 2012-03-12 2017-05-23 Dsm Ip Assets B.V. Recombination system
CN105324488A (zh) 2013-06-21 2016-02-10 诺维信公司 芽孢杆菌属中不具有分泌信号的多肽的生产
EP3019607B2 (de) * 2013-07-12 2022-09-07 Novozymes A/S Direkter transport von polynucleotiden zwischen genomen
CN107624134A (zh) 2015-05-12 2018-01-23 诺维信公司 缺失羊毛硫抗生素基因的地衣芽孢杆菌宿主细胞
CN106906238B (zh) * 2015-12-22 2020-08-14 中国科学院分子植物科学卓越创新中心 一种链霉菌抗生素生物合成基因簇多拷贝扩增方法及应用
CN109804072A (zh) * 2016-10-25 2019-05-24 诺维信公司 地衣芽孢杆菌中flp介导的基因组整合
CN108251344A (zh) * 2016-12-28 2018-07-06 中国科学院上海生命科学研究院 系列天蓝色链霉菌高效异源表达宿主的构建与应用
WO2018134386A1 (en) 2017-01-23 2018-07-26 Novozymes A/S Host cells and methods for producing double-stranded rna
CN111417726A (zh) * 2017-10-23 2020-07-14 诺维信公司 通过与前肽共表达改善蛋白酶的表达
CN111511908A (zh) 2017-11-10 2020-08-07 诺维信公司 温度敏感性cas9蛋白
US20210017544A1 (en) 2017-12-22 2021-01-21 Novozymes A/S Counter-Selection by Inhibition of Conditionally Essential Genes
WO2020229191A1 (en) 2019-05-15 2020-11-19 Novozymes A/S Temperature-sensitive rna-guided endonuclease
CN114207125A (zh) 2019-06-25 2022-03-18 诺维信公司 通过抑制条件性必需基因进行反向选择
EP4133086A4 (de) * 2020-04-07 2024-06-05 IO Biosciences, Inc. Nukleinsäurekonstrukte mit geneditierenden multi-sites
BR112023027016A2 (pt) 2021-06-24 2024-03-12 Basf Se Célula hospedeira modificada de bacillus compreendendo uma proteína reguladora da matriz extracelular a alterada e/ou uma proteína reguladora da matriz extracelular b alterada, método para produzir um composto de interesse, e, proteínas reguladoras das matrizes extracelulares a e b alteradas
CN117730090A (zh) 2021-07-13 2024-03-19 诺维信公司 重组角质酶表达
WO2023064778A1 (en) * 2021-10-11 2023-04-20 The Board Of Trustees Of The Leland Stanford Junior University Dna element responsive to extrachromosomal dna in cancer cells
CN118339276A (zh) 2021-12-10 2024-07-12 诺维信公司 重组细菌中改善的蛋白产量
WO2024120767A1 (en) 2022-12-05 2024-06-13 Novozymes A/S Modified rna polymerase activities

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2563533B1 (fr) 1984-04-27 1986-08-22 Centre Nat Rech Scient Procede d'amplification de l'expression d'un gene determine chez bacillus subtilis et souches obtenues
JP2637532B2 (ja) 1987-02-27 1997-08-06 ギスト ブロカデス ナームローゼ フェンノートチャップ 原核微生物の染色体ｄｎａにおける安定した遺伝子増幅
US5470727A (en) * 1993-12-21 1995-11-28 Celtrix Pharmaceuticals, Inc. Chromosomal expression of non-bacterial genes in bacterial cells
US6017694A (en) * 1997-12-19 2000-01-25 American Cyanamid Company Methods of screening for modulators of respiratory syncytial virus matrix protein interaction
US5955310A (en) 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
JP2002522013A (ja) * 1998-07-24 2002-07-23 ベイラ、カリッジ、アヴ、メダスン 部位特異的組換えを使用する迅速なサブクローニング
CN1183257C (zh) * 1999-10-29 2005-01-05 宝生物工程株式会社 基因转移的方法
WO2002000907A1 (en) 2000-06-23 2002-01-03 Novozymes A/S Method for stable chromosomal multi-copy integration of genes
IL154063A0 (en) * 2000-07-21 2003-07-31 Us Agriculture Methods for the replacement, translocation and stacking of dna in eukaryotic genomes
US20040247620A1 (en) 2002-08-21 2004-12-09 Kosan Biosciences, Inc. Transformation system based on the integrase gene and attachment site for Myxococcus xanthus bacteriophage Mx9
EP1405908A1 (de) * 2002-10-04 2004-04-07 ProBioGen AG Herstellung von Zelllinien zur heterologen Expression
US20040209370A1 (en) * 2002-12-19 2004-10-21 Wonchul Suh Method for chromosomal engineering
EP1805296B1 (de) * 2004-10-22 2009-09-16 Novozymes A/S Stabile genomische integration mehrerer polynukleotidkopien
EP2527448A1 (de) * 2011-05-23 2012-11-28 Novozymes A/S Gleichzeitige standortspezifische Integrationen von mehreren Genkopien in faserige Pilze

Also Published As

Publication number Publication date
ATE443128T1 (de) 2009-10-15
DK1805296T3 (da) 2010-01-18
US11174487B2 (en) 2021-11-16
EP1805296A1 (de) 2007-07-11
US20170218381A1 (en) 2017-08-03
CN101061214A (zh) 2007-10-24
EP1805296B1 (de) 2009-09-16
US20140273237A1 (en) 2014-09-18
CN101061214B (zh) 2012-12-05
WO2006042548A1 (en) 2006-04-27
US20080085535A1 (en) 2008-04-10

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