DE602004026912D1 - Formulierungen und verfahren zum denaturieren von proteinen - Google Patents
Formulierungen und verfahren zum denaturieren von proteinenInfo
- Publication number
- DE602004026912D1 DE602004026912D1 DE602004026912T DE602004026912T DE602004026912D1 DE 602004026912 D1 DE602004026912 D1 DE 602004026912D1 DE 602004026912 T DE602004026912 T DE 602004026912T DE 602004026912 T DE602004026912 T DE 602004026912T DE 602004026912 D1 DE602004026912 D1 DE 602004026912D1
- Authority
- DE
- Germany
- Prior art keywords
- formulations
- denaturing proteins
- denaturing
- proteins
- denaturation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/99—Enzyme inactivation by chemical treatment
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/814—Enzyme separation or purification
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
- Y10T436/255—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.] including use of a solid sorbent, semipermeable membrane, or liquid extraction
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Analytical Chemistry (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US53039203P | 2003-12-16 | 2003-12-16 | |
PCT/US2004/042044 WO2005058933A1 (en) | 2003-12-16 | 2004-12-15 | Formulations and methods for denaturing proteins |
Publications (1)
Publication Number | Publication Date |
---|---|
DE602004026912D1 true DE602004026912D1 (de) | 2010-06-10 |
Family
ID=34700134
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE602004026912T Active DE602004026912D1 (de) | 2003-12-16 | 2004-12-15 | Formulierungen und verfahren zum denaturieren von proteinen |
Country Status (7)
Country | Link |
---|---|
US (1) | US7115719B2 (de) |
EP (1) | EP1694692B1 (de) |
JP (1) | JP4435787B2 (de) |
AT (1) | ATE466080T1 (de) |
CA (1) | CA2549985C (de) |
DE (1) | DE602004026912D1 (de) |
WO (1) | WO2005058933A1 (de) |
Families Citing this family (23)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2304017C (en) * | 1997-09-17 | 2007-05-01 | Gentra Systems, Inc. | Apparatuses and methods for isolating nucleic acid |
US20050032105A1 (en) * | 2001-10-12 | 2005-02-10 | Bair Robert Jackson | Compositions and methods for using a solid support to purify DNA |
US7148343B2 (en) * | 2001-10-12 | 2006-12-12 | Gentra Systems, Inc. | Compositions and methods for using a solid support to purify RNA |
US7893228B2 (en) * | 2001-10-12 | 2011-02-22 | Qiagen North American Holdings, Inc. | Compositions and methods for using a solid support to purify RNA |
WO2005093052A1 (en) * | 2004-03-26 | 2005-10-06 | Fuji Photo Film Co., Ltd. | Method for selectively separating and purifying rna and method for separating and purifying nucleic acid |
CN101124321B (zh) | 2004-11-05 | 2012-02-29 | 恰根北美控股有限公司 | 用于从稳定剂中纯化核酸的组合物和方法 |
US20070092403A1 (en) * | 2005-10-21 | 2007-04-26 | Alan Wirbisky | Compact apparatus, compositions and methods for purifying nucleic acids |
DE102005059217B4 (de) * | 2005-12-07 | 2011-03-17 | Aj Innuscreen Gmbh | Verfahren und Testkit zur Trennung, Aufreinigung und Wiedergewinnung von lang- und kurzkettigen Nukleinsäuren |
JP4825528B2 (ja) * | 2006-02-03 | 2011-11-30 | 富士フイルム株式会社 | 核酸の分離精製方法 |
US8962262B2 (en) * | 2006-05-11 | 2015-02-24 | Arbor Vita Corporation | Method of protein extraction from cells |
US9207240B2 (en) | 2007-11-14 | 2015-12-08 | Arbor Vita Corporation | Method of efficient extraction of protein from cells |
EP2281826B1 (de) * | 2008-04-02 | 2016-02-17 | Rbc Bioscience Corp. | Auf magnetischen cellulosematerialien basierende reagentien, chaotrope mittel, kits und methoden zur isolierung von nukleinsäuren |
GB201217405D0 (en) | 2012-09-28 | 2012-11-14 | Fermentas Uab | Protein removal agent |
EP3467108B1 (de) * | 2013-03-14 | 2024-05-22 | Translate Bio, Inc. | Verfahren zur reinigung von messenger-rna |
WO2014144767A1 (en) * | 2013-03-15 | 2014-09-18 | Moderna Therapeutics, Inc. | Ion exchange purification of mrna |
EP2971033B8 (de) | 2013-03-15 | 2019-07-10 | ModernaTX, Inc. | Herstellungsverfahren zur herstellung von rna-transkripten |
US10077439B2 (en) | 2013-03-15 | 2018-09-18 | Modernatx, Inc. | Removal of DNA fragments in mRNA production process |
US11377470B2 (en) | 2013-03-15 | 2022-07-05 | Modernatx, Inc. | Ribonucleic acid purification |
CN117402871A (zh) | 2014-04-25 | 2024-01-16 | 川斯勒佰尔公司 | 信使rna的纯化方法 |
DK3294885T3 (da) | 2015-05-08 | 2020-08-10 | Curevac Real Estate Gmbh | Fremgangsmåde til at fremstille rna |
EP4108769B1 (de) | 2015-05-29 | 2023-08-30 | CureVac Manufacturing GmbH | Verfahren zur herstellung und reinigung von rna mit mindestens einem schritt mit einer tangentialen flussfiltration |
US11174500B2 (en) | 2018-08-24 | 2021-11-16 | Translate Bio, Inc. | Methods for purification of messenger RNA |
CN110229225B (zh) * | 2019-06-12 | 2021-07-20 | 山东师范大学 | 一种南极磷虾中富硫和/或富铯蛋白的制备方法 |
Family Cites Families (30)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS594664B2 (ja) * | 1976-11-10 | 1984-01-31 | 株式会社日立製作所 | イオン交換クロマトグラフイ− |
US5599667A (en) * | 1987-03-02 | 1997-02-04 | Gen-Probe Incorporated | Polycationic supports and nucleic acid purification separation and hybridization |
US4843155A (en) * | 1987-11-19 | 1989-06-27 | Piotr Chomczynski | Product and process for isolating RNA |
US5234809A (en) * | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
US5010183A (en) * | 1989-07-07 | 1991-04-23 | Macfarlane Donald E | Process for purifying DNA and RNA using cationic detergents |
DE4034036C2 (de) * | 1990-10-26 | 1994-03-03 | Diagen Inst Molekularbio | Vorrichtung und Verfahren zur Isolierung von Nukleinsäuren aus Zellsuspensionen |
US5652141A (en) * | 1990-10-26 | 1997-07-29 | Oiagen Gmbh | Device and process for isolating nucleic acids from cell suspension |
GB9108604D0 (en) * | 1991-04-22 | 1991-06-05 | Nadreph Ltd | Gel products and a process for making them |
CA2067711C (en) * | 1991-05-03 | 2000-08-08 | Daniel Lee Woodard | Solid phase extraction purification of dna |
US5422241A (en) * | 1991-07-03 | 1995-06-06 | Ambion, Inc. | Methods for the recovery of nucleic acids from reaction mixtures |
US5346994A (en) * | 1992-01-28 | 1994-09-13 | Piotr Chomczynski | Shelf-stable product and process for isolating RNA, DNA and proteins |
JP3615545B2 (ja) * | 1993-02-01 | 2005-02-02 | キアゲン・エヌ・ブイ | 第四級アンモニウム塩界面活性剤及びそのrnaの単離剤 |
DE4321904B4 (de) * | 1993-07-01 | 2013-05-16 | Qiagen Gmbh | Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen |
GB9314249D0 (en) | 1993-07-09 | 1993-08-18 | Proofname Ltd | Purification method and apparatus |
EP0673638A3 (de) * | 1994-03-24 | 1996-10-16 | Kao Corp | Antikörper enthaltende kosmetische Zusammensetzung. |
JP3761573B2 (ja) * | 1994-06-14 | 2006-03-29 | インヴィテーク ゲーエムベーハー | 極度に少量でかつ非常に強く汚染された非常に種々の出発物質から核酸を単離および精製する一般的方法 |
JP3163224B2 (ja) * | 1994-10-14 | 2001-05-08 | 三菱レイヨン株式会社 | 菌体または固定化菌体の懸濁液の保存方法 |
US5945515A (en) * | 1995-07-31 | 1999-08-31 | Chomczynski; Piotr | Product and process for isolating DNA, RNA and proteins |
US5973137A (en) * | 1996-02-13 | 1999-10-26 | Gentra Systems, Inc. | Low pH RNA isolation reagents, method, and kit |
US5677124A (en) * | 1996-07-03 | 1997-10-14 | Ambion, Inc. | Ribonuclease resistant viral RNA standards |
US5939262A (en) * | 1996-07-03 | 1999-08-17 | Ambion, Inc. | Ribonuclease resistant RNA preparation and utilization |
EP0818461B1 (de) * | 1996-07-12 | 2005-09-28 | Toyo Boseki Kabushiki Kaisha | Verfahren zur Isolierung von Ribonucleinsäuren. |
CA2304017C (en) * | 1997-09-17 | 2007-05-01 | Gentra Systems, Inc. | Apparatuses and methods for isolating nucleic acid |
US6204375B1 (en) * | 1998-07-31 | 2001-03-20 | Ambion, Inc. | Methods and reagents for preserving RNA in cell and tissue samples |
US6777210B1 (en) * | 1998-09-24 | 2004-08-17 | Ambion, Inc. | Method and reagents for inactivating ribonucleases RNase A, RNase I and RNase T1 |
US6811788B2 (en) * | 2000-01-19 | 2004-11-02 | Baofa Yu | Combinations and methods for treating neoplasms |
US7893228B2 (en) * | 2001-10-12 | 2011-02-22 | Qiagen North American Holdings, Inc. | Compositions and methods for using a solid support to purify RNA |
AU2002211719B2 (en) | 2001-10-12 | 2007-06-14 | Qiagen North American Holdings, Inc. | Compositions and methods for using a solid support to purify RNA |
US20050032105A1 (en) * | 2001-10-12 | 2005-02-10 | Bair Robert Jackson | Compositions and methods for using a solid support to purify DNA |
US7148343B2 (en) * | 2001-10-12 | 2006-12-12 | Gentra Systems, Inc. | Compositions and methods for using a solid support to purify RNA |
-
2004
- 2004-12-15 AT AT04814250T patent/ATE466080T1/de not_active IP Right Cessation
- 2004-12-15 EP EP04814250A patent/EP1694692B1/de active Active
- 2004-12-15 CA CA2549985A patent/CA2549985C/en active Active
- 2004-12-15 US US11/012,527 patent/US7115719B2/en active Active
- 2004-12-15 JP JP2006545361A patent/JP4435787B2/ja active Active
- 2004-12-15 DE DE602004026912T patent/DE602004026912D1/de active Active
- 2004-12-15 WO PCT/US2004/042044 patent/WO2005058933A1/en not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
US7115719B2 (en) | 2006-10-03 |
US20050171333A1 (en) | 2005-08-04 |
JP2007515959A (ja) | 2007-06-21 |
EP1694692B1 (de) | 2010-04-28 |
CA2549985A1 (en) | 2005-06-30 |
CA2549985C (en) | 2011-05-24 |
WO2005058933A1 (en) | 2005-06-30 |
EP1694692A1 (de) | 2006-08-30 |
ATE466080T1 (de) | 2010-05-15 |
JP4435787B2 (ja) | 2010-03-24 |
EP1694692A4 (de) | 2008-07-23 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition |