DE60118350D1 - Fluoreszenz-verfahren zum nachweis von proteasen oder peptidasen - Google Patents

Fluoreszenz-verfahren zum nachweis von proteasen oder peptidasen

Info

Publication number
DE60118350D1
DE60118350D1 DE60118350T DE60118350T DE60118350D1 DE 60118350 D1 DE60118350 D1 DE 60118350D1 DE 60118350 T DE60118350 T DE 60118350T DE 60118350 T DE60118350 T DE 60118350T DE 60118350 D1 DE60118350 D1 DE 60118350D1
Authority
DE
Germany
Prior art keywords
peptidases
detecting
proteases
fluorescent
procedure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60118350T
Other languages
English (en)
Other versions
DE60118350T2 (de
Inventor
Pierre Roques
Nathalie Luciani
Marie-Claude Fournie-Zaluski
Rocquigny Hugues De
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Institut National de la Sante et de la Recherche Medicale INSERM
Original Assignee
Centre National de la Recherche Scientifique CNRS
Institut National de la Sante et de la Recherche Medicale INSERM
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS, Institut National de la Sante et de la Recherche Medicale INSERM filed Critical Centre National de la Recherche Scientifique CNRS
Publication of DE60118350D1 publication Critical patent/DE60118350D1/de
Application granted granted Critical
Publication of DE60118350T2 publication Critical patent/DE60118350T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/575—Hormones
    • G01N2333/5754—Endothelin, vasoactive intestinal contractor [VIC]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
    • G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
    • G01N2333/96486—Metalloendopeptidases (3.4.24)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/968—High energy substrates, e.g. fluorescent, chemiluminescent, radioactive

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
DE60118350T 2000-04-07 2001-04-06 Fluoreszenz-verfahren zum nachweis von proteasen oder peptidasen Expired - Lifetime DE60118350T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR0004507A FR2807523B1 (fr) 2000-04-07 2000-04-07 UTILISATION D'UN RESTE PyA-(Z)x-pNF A DES FINS DE DETECTION, IDENTIFICATION ET/OU DOSAGE PAR FLUORESCENCE
FR0004507 2000-04-07
PCT/FR2001/001058 WO2001077369A1 (fr) 2000-04-07 2001-04-06 Detection de proteases ou peptidases par fluorescence

Publications (2)

Publication Number Publication Date
DE60118350D1 true DE60118350D1 (de) 2006-05-18
DE60118350T2 DE60118350T2 (de) 2006-10-19

Family

ID=8849025

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60118350T Expired - Lifetime DE60118350T2 (de) 2000-04-07 2001-04-06 Fluoreszenz-verfahren zum nachweis von proteasen oder peptidasen

Country Status (9)

Country Link
US (1) US7169574B2 (de)
EP (1) EP1268850B1 (de)
JP (1) JP4966469B2 (de)
AT (1) ATE321877T1 (de)
CA (1) CA2405183C (de)
DE (1) DE60118350T2 (de)
ES (1) ES2256224T3 (de)
FR (1) FR2807523B1 (de)
WO (1) WO2001077369A1 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2903495A1 (fr) * 2006-07-06 2008-01-11 Pharmaleads Soc Par Actions Si PROCEDE DE DETECTION D'INTERACTIONS A L'AIDE DU RAPPORTEUR DE FLUORESCENCE Pya
FR2931151A1 (fr) * 2008-05-13 2009-11-20 Pharmaleads Soc Par Actions Si Nouveaux derives d'amino-acides, leur procede de preparation et leur utilisation therapeutique
FR2963022B1 (fr) * 2010-07-21 2012-08-31 Pharmaleads Nouveaux substrats a fluorescence reprimee, leur preparation et leur utilisation pour l'identification, la detection et le dosage de legionella pneumophila
CN115047092B (zh) * 2022-04-07 2023-10-20 济宁医学院 一种血管紧张素转移酶ii抑制剂的筛选方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4966848A (en) * 1988-02-08 1990-10-30 The General Hospital Corporation Isolation, purification, characterization, cloning and sequencing of N α-acetyltransferase
US5223421A (en) * 1989-10-25 1993-06-29 The General Hospital Corporation Identification of methionine Nα-acetyltransferase
DE4313232A1 (de) * 1993-04-22 1994-10-27 Basf Ag Methode zur Bestimmung der Aktivität des Endothelin-Konversionsenzyms (ECE)
US5506115A (en) * 1994-04-29 1996-04-09 G. D. Searle & Co. Reagent and method for determining activity of herpes protease
US5688489A (en) * 1995-09-15 1997-11-18 Resolution Pharmaceuticals, Inc. Non-receptor mediated imaging agents
FR2745576B1 (fr) * 1996-03-04 1998-06-19 Inst Nat Sante Rech Med Nouveaux peptides radioactifs, leur preparation, et leur utilisation pour le test de selection d'inhibiteurs de l'enzyme de conversion de l'endotheline

Also Published As

Publication number Publication date
EP1268850B1 (de) 2006-03-29
ES2256224T3 (es) 2006-07-16
US20040214256A1 (en) 2004-10-28
CA2405183A1 (fr) 2001-10-18
US7169574B2 (en) 2007-01-30
FR2807523B1 (fr) 2002-08-30
DE60118350T2 (de) 2006-10-19
EP1268850A1 (de) 2003-01-02
JP4966469B2 (ja) 2012-07-04
JP2003530370A (ja) 2003-10-14
CA2405183C (fr) 2012-06-05
FR2807523A1 (fr) 2001-10-12
WO2001077369A1 (fr) 2001-10-18
ATE321877T1 (de) 2006-04-15

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition