DE60044223D1 - Durch oligonukleotide-vermittelte nukleinsäuren-rekombination - Google Patents
Durch oligonukleotide-vermittelte nukleinsäuren-rekombinationInfo
- Publication number
- DE60044223D1 DE60044223D1 DE60044223T DE60044223T DE60044223D1 DE 60044223 D1 DE60044223 D1 DE 60044223D1 DE 60044223 T DE60044223 T DE 60044223T DE 60044223 T DE60044223 T DE 60044223T DE 60044223 D1 DE60044223 D1 DE 60044223D1
- Authority
- DE
- Germany
- Prior art keywords
- nucleic acid
- oligonucleotide
- mediated nucleic
- acid recombination
- oligonucleotides
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108020004707 nucleic acids Proteins 0.000 title abstract 5
- 150000007523 nucleic acids Chemical class 0.000 title abstract 5
- 102000039446 nucleic acids Human genes 0.000 title abstract 5
- 230000006798 recombination Effects 0.000 title 1
- 238000005215 recombination Methods 0.000 title 1
- 108091034117 Oligonucleotide Proteins 0.000 abstract 3
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 3
- 102000003960 Ligases Human genes 0.000 abstract 1
- 108090000364 Ligases Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/0068—Means for controlling the apparatus of the process
- B01J2219/00686—Automatic
- B01J2219/00689—Automatic using computers
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (9)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US11644799P | 1999-01-19 | 1999-01-19 | |
US11881399P | 1999-02-05 | 1999-02-05 | |
US11885499P | 1999-02-05 | 1999-02-05 | |
US14104999P | 1999-06-24 | 1999-06-24 | |
US09/408,392 US6376246B1 (en) | 1999-02-05 | 1999-09-28 | Oligonucleotide mediated nucleic acid recombination |
US09/408,393 US6436675B1 (en) | 1999-09-28 | 1999-09-28 | Use of codon-varied oligonucleotide synthesis for synthetic shuffling |
US41683799A | 1999-10-12 | 1999-10-12 | |
US41637599A | 1999-10-12 | 1999-10-12 | |
PCT/US2000/001203 WO2000042561A2 (en) | 1999-01-19 | 2000-01-18 | Oligonucleotide mediated nucleic acid recombination |
Publications (1)
Publication Number | Publication Date |
---|---|
DE60044223D1 true DE60044223D1 (de) | 2010-06-02 |
Family
ID=27574833
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE60044223T Expired - Lifetime DE60044223D1 (de) | 1999-01-19 | 2000-01-18 | Durch oligonukleotide-vermittelte nukleinsäuren-rekombination |
Country Status (11)
Country | Link |
---|---|
US (1) | US7058515B1 (de) |
EP (6) | EP1130093A1 (de) |
JP (2) | JP2002534966A (de) |
AT (1) | ATE465459T1 (de) |
AU (2) | AU3210200A (de) |
CA (2) | CA2320714A1 (de) |
DE (1) | DE60044223D1 (de) |
DK (1) | DK1072010T3 (de) |
ES (1) | ES2341217T3 (de) |
IL (2) | IL138002A0 (de) |
WO (2) | WO2000042560A2 (de) |
Families Citing this family (215)
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2000
- 2000-01-18 DE DE60044223T patent/DE60044223D1/de not_active Expired - Lifetime
- 2000-01-18 IL IL13800200A patent/IL138002A0/xx unknown
- 2000-01-18 EP EP01102019A patent/EP1130093A1/de not_active Withdrawn
- 2000-01-18 EP EP01102038A patent/EP1108783A3/de not_active Withdrawn
- 2000-01-18 CA CA002320714A patent/CA2320714A1/en not_active Abandoned
- 2000-01-18 JP JP2000594068A patent/JP2002534966A/ja active Pending
- 2000-01-18 EP EP00909922A patent/EP1062614A1/de not_active Withdrawn
- 2000-01-18 AU AU32102/00A patent/AU3210200A/en not_active Abandoned
- 2000-01-18 EP EP01102660A patent/EP1108781A3/de not_active Withdrawn
- 2000-01-18 EP EP00909923A patent/EP1072010B1/de not_active Expired - Lifetime
- 2000-01-18 DK DK00909923.5T patent/DK1072010T3/da active
- 2000-01-18 AT AT00909923T patent/ATE465459T1/de not_active IP Right Cessation
- 2000-01-18 ES ES00909923T patent/ES2341217T3/es not_active Expired - Lifetime
- 2000-01-18 CA CA2320697A patent/CA2320697C/en not_active Expired - Fee Related
- 2000-01-18 EP EP10075153.6A patent/EP2253704B1/de not_active Expired - Lifetime
- 2000-01-18 JP JP2000594067A patent/JP2002534965A/ja not_active Withdrawn
- 2000-01-18 IL IL13786800A patent/IL137868A0/xx unknown
- 2000-01-18 WO PCT/US2000/001202 patent/WO2000042560A2/en not_active Application Discontinuation
- 2000-01-18 AU AU32101/00A patent/AU3210100A/en not_active Abandoned
- 2000-01-18 WO PCT/US2000/001203 patent/WO2000042561A2/en not_active Application Discontinuation
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Also Published As
Publication number | Publication date |
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EP2253704A1 (de) | 2010-11-24 |
WO2000042560A2 (en) | 2000-07-20 |
EP1108781A2 (de) | 2001-06-20 |
WO2000042560A3 (en) | 2000-11-23 |
JP2002534965A (ja) | 2002-10-22 |
US7058515B1 (en) | 2006-06-06 |
ES2341217T3 (es) | 2010-06-17 |
WO2000042561A2 (en) | 2000-07-20 |
EP1108783A2 (de) | 2001-06-20 |
JP2002534966A (ja) | 2002-10-22 |
DK1072010T3 (da) | 2010-06-21 |
EP1072010A2 (de) | 2001-01-31 |
EP1130093A1 (de) | 2001-09-05 |
ATE465459T1 (de) | 2010-05-15 |
CA2320697A1 (en) | 2000-07-20 |
EP2253704B1 (de) | 2015-08-19 |
IL138002A0 (en) | 2001-10-31 |
EP1108783A3 (de) | 2001-09-05 |
CA2320714A1 (en) | 2000-07-20 |
WO2000042561A3 (en) | 2000-12-07 |
EP1108781A3 (de) | 2001-09-12 |
AU3210100A (en) | 2000-08-01 |
AU3210200A (en) | 2000-08-01 |
EP1072010B1 (de) | 2010-04-21 |
IL137868A0 (en) | 2001-10-31 |
CA2320697C (en) | 2014-11-18 |
EP1062614A1 (de) | 2000-12-27 |
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