DE60033842D1 - MALE STERILE BRASSICA PLANTS AND ITS MANUFACTURE - Google Patents

MALE STERILE BRASSICA PLANTS AND ITS MANUFACTURE

Info

Publication number
DE60033842D1
DE60033842D1 DE60033842T DE60033842T DE60033842D1 DE 60033842 D1 DE60033842 D1 DE 60033842D1 DE 60033842 T DE60033842 T DE 60033842T DE 60033842 T DE60033842 T DE 60033842T DE 60033842 D1 DE60033842 D1 DE 60033842D1
Authority
DE
Germany
Prior art keywords
manufacture
male sterile
brassica plants
sterile brassica
construct
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60033842T
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German (de)
Other versions
DE60033842T2 (en
Inventor
Brigitte Weston
Beuckeleer Marc De
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer CropScience NV
Original Assignee
Bayer Bioscience NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer Bioscience NV filed Critical Bayer Bioscience NV
Publication of DE60033842D1 publication Critical patent/DE60033842D1/en
Application granted granted Critical
Publication of DE60033842T2 publication Critical patent/DE60033842T2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289—Male sterility

Abstract

This invention pertains to methods for determining the presence or absence in Brassica plant material of elite event MS-B2, which is a specific transformation event of a construct comprising a male-sterility gene, at a specific location in the Brassica genome, using PCR and primers specific to the construct, and kits for use in the methods.
DE60033842T 1999-10-29 2000-10-26 MALE STERILE BRASSICA PLANTS AND ITS MANUFACTURE Expired - Lifetime DE60033842T2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/430,497 US6509516B1 (en) 1999-10-29 1999-10-29 Male-sterile brassica plants and methods for producing same
US430497 1999-10-29
PCT/EP2000/010680 WO2001031042A2 (en) 1999-10-29 2000-10-26 Male-sterile brassica plants and methods for producing same

Publications (2)

Publication Number Publication Date
DE60033842D1 true DE60033842D1 (en) 2007-04-19
DE60033842T2 DE60033842T2 (en) 2007-11-08

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
DE60033842T Expired - Lifetime DE60033842T2 (en) 1999-10-29 2000-10-26 MALE STERILE BRASSICA PLANTS AND ITS MANUFACTURE

Country Status (8)

Country Link
US (2) US6509516B1 (en)
EP (1) EP1228227B1 (en)
AT (1) ATE356218T1 (en)
AU (1) AU783410B2 (en)
CA (1) CA2388860C (en)
DE (1) DE60033842T2 (en)
DK (1) DK1228227T3 (en)
WO (1) WO2001031042A2 (en)

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