DE60027770D1 - Chemisch induzierbarer promoter zur gewinnung von transgenen pflanzen mit stillem marker sowie pflanzen und verfahren die diese benutzen - Google Patents

Chemisch induzierbarer promoter zur gewinnung von transgenen pflanzen mit stillem marker sowie pflanzen und verfahren die diese benutzen

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Publication number
DE60027770D1
DE60027770D1 DE60027770T DE60027770T DE60027770D1 DE 60027770 D1 DE60027770 D1 DE 60027770D1 DE 60027770 T DE60027770 T DE 60027770T DE 60027770 T DE60027770 T DE 60027770T DE 60027770 D1 DE60027770 D1 DE 60027770D1
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DE
Germany
Prior art keywords
plants
promoter
inducer
cells
genes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE60027770T
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English (en)
Other versions
DE60027770T2 (de
Inventor
Jianru Zuo
Nam-Hai Chua
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Rockefeller University
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Rockefeller University
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Publication of DE60027770D1 publication Critical patent/DE60027770D1/de
Application granted granted Critical
Publication of DE60027770T2 publication Critical patent/DE60027770T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8291—Hormone-influenced development
    • C12N15/8295—Cytokinins

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Endocrinology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
DE60027770T 1999-11-12 2000-11-13 Chemisch induzierbarer promoter zur gewinnung von transgenen pflanzen mit stillem marker sowie pflanzen und verfahren die diese benutzen Expired - Fee Related DE60027770T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/438,392 US6784340B1 (en) 1998-01-28 1999-11-12 Chemical inducible promoter used to obtain transgenic plants with a silent marker
US438392 1999-11-12
PCT/US2000/031070 WO2001034821A2 (en) 1999-11-12 2000-11-13 Chemical inducible promoter used to obtain transgenic plants with a silent marker and organisms and cells and methods of using same for screening for mutations

Publications (2)

Publication Number Publication Date
DE60027770D1 true DE60027770D1 (de) 2006-06-08
DE60027770T2 DE60027770T2 (de) 2006-11-02

Family

ID=23740478

Family Applications (1)

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DE60027770T Expired - Fee Related DE60027770T2 (de) 1999-11-12 2000-11-13 Chemisch induzierbarer promoter zur gewinnung von transgenen pflanzen mit stillem marker sowie pflanzen und verfahren die diese benutzen

Country Status (9)

Country Link
US (2) US6784340B1 (de)
EP (1) EP1242604B1 (de)
CN (1) CN1249247C (de)
AT (1) ATE325199T1 (de)
AU (1) AU782958B2 (de)
CA (1) CA2388755A1 (de)
DE (1) DE60027770T2 (de)
ES (1) ES2263502T3 (de)
WO (1) WO2001034821A2 (de)

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US7446241B2 (en) * 2002-07-30 2008-11-04 Texas Tech University Transcription factors, DNA and methods for introduction of value-added seed traits and stress tolerance
WO2005033306A1 (ja) 2003-10-01 2005-04-14 Japan Science And Technology Agency 形質転換細胞および該細胞を用いたタンパク質の生産方法並びにタンパク質生産キット、ウイルスベクター発現用ｄｎａ断片およびその利用、タンパク質生産用形質転換体の生産方法および当該生産方法によって得られたタンパク質生産用形質転換体並びにその利用
US7737327B2 (en) * 2004-03-09 2010-06-15 The University Of Kentucky Research Foundation Methods for screening for genes and small molecules that activate mammalian receptor proteins
US7148402B2 (en) * 2004-05-21 2006-12-12 Rockefeller University Promotion of somatic embryogenesis in plants by PGA37 gene expression
US8852928B2 (en) * 2007-07-06 2014-10-07 Temasek Life Sciences Laboratory Limited LexA-based chemical-inducible gene expression system for use in transgenic animals
EP2183355B1 (de) * 2007-09-17 2016-01-27 Rohm and Haas Company Zusammensetzungen und verfahren zur modifizierung physiologischer reaktionen in pflanzen
CA2752697A1 (en) * 2009-03-05 2010-09-10 Rohm And Haas Company Control of targeted turnover of key ethylene hormone signaling pathway proteins to modulate ethylene sensitivity in plants
US9145562B2 (en) * 2009-11-20 2015-09-29 Alberta Innovates—Technology Futures Variegation in plants
US10677785B2 (en) * 2010-05-24 2020-06-09 Plant Response, Inc. Reporter constructs for compound screening
WO2011149843A1 (en) * 2010-05-24 2011-12-01 Mendel Biotechnology Inc. Novel reporter constructs for compound screening
FI124176B (en) 2011-12-01 2014-04-15 Helsingin Yliopisto Polypeptide, polynucleotide, expression cassette, vector, host cell, plant and uses
EP2951298A1 (de) 2013-01-29 2015-12-09 The University Court of The University Of Glasgow Verfahren und mittels zur erhöhung der stresstoleranz und biomasse in pflanzen
AU2014286296B2 (en) 2013-07-01 2020-04-09 Basf Se Methods and means for modulating flowering time in monocot plants
EP3431601B1 (de) 2013-07-10 2022-09-07 Basf Se Rnai zur bekämpfung von phytopathogenen pilzen und eipilzen durch hemmung der expression des cyp51-gens
ES2912283T3 (es) 2013-12-11 2022-05-25 Massachusetts Gen Hospital Uso de proteínas de tipo sustancia inhibidora mülleriana (SIM) para la anticoncepción y conservación de la reserva ovárica
WO2015153964A1 (en) * 2014-04-04 2015-10-08 University Of Tennessee Research Foundation Glyphosate-inducible plant promoter and uses thereof
ES2773601T3 (es) 2014-07-22 2020-07-13 Nmc Inc Sistemas de fijación de carbono mejorados en plantas y algas
WO2016050512A1 (en) 2014-10-03 2016-04-07 Bayer Cropscience Nv Methods and means for increasing stress tolerance and biomass in plants
US20190225974A1 (en) 2016-09-23 2019-07-25 BASF Agricultural Solutions Seed US LLC Targeted genome optimization in plants
WO2018102656A1 (en) * 2016-12-02 2018-06-07 City Of Hope Producing recombinant products in contained systems
EP3737690A1 (de) 2018-01-12 2020-11-18 Basf Se Gen, das unter der anzahl der spikelets pro spike-qtl in weizen auf chromosom 7a liegt
WO2019224359A1 (en) 2018-05-25 2019-11-28 Basf Se Plants comprising wheat g-type cytoplasmic male sterility restorer genes and uses thereof
EA202092647A1 (ru) 2018-05-25 2021-07-08 Басф Се Растения, содержащие гены-восстановители для цитоплазматической мужской стерильности g-типа пшеницы, и их применение
US20210214746A1 (en) 2018-05-25 2021-07-15 Basf Se Plants comprising wheat g-type cytoplasmic male sterility restorer genes and uses thereof
WO2019234231A1 (en) 2018-06-08 2019-12-12 Basf Se Plants comprising wheat g-type cytoplasmic male sterility restorer genes and uses thereof
JP2022531140A (ja) 2019-04-25 2022-07-06 インポッシブル フーズ インコーポレイテッド ヘム含有タンパク質の作製のための株および方法
CN110387383B (zh) * 2019-07-30 2022-12-06 贵州省烟草科学研究院 一种调控NtCBT基因在烟草组织中表达的方法及其应用

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US5614395A (en) * 1988-03-08 1997-03-25 Ciba-Geigy Corporation Chemically regulatable and anti-pathogenic DNA sequences and uses thereof
DE3810286A1 (de) 1988-03-25 1989-10-12 Max Planck Gesellschaft Transgene pflanze mit modifizierter physiologie, morphologie und modifiziertem hormonmetabolismus, gewebekulturen dieser pflanze und verfahren zu ihrer herstellung
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US5952543A (en) 1997-02-25 1999-09-14 Dna Plant Technology Corporation Genetically transformed pineapple plants and methods for their production
US6063985A (en) 1998-01-28 2000-05-16 The Rockefeller University Chemical inducible promotor used to obtain transgenic plants with a silent marker
US6452068B1 (en) * 1998-01-28 2002-09-17 The Rockefeller University Chemical inducible promoters used to obtain transgenic plants with a silent marker

Also Published As

Publication number Publication date
CA2388755A1 (en) 2001-05-17
ATE325199T1 (de) 2006-06-15
EP1242604A2 (de) 2002-09-25
AU1485601A (en) 2001-06-06
WO2001034821A3 (en) 2002-05-30
CN1423702A (zh) 2003-06-11
ES2263502T3 (es) 2006-12-16
CN1249247C (zh) 2006-04-05
US6784340B1 (en) 2004-08-31
WO2001034821A2 (en) 2001-05-17
DE60027770T2 (de) 2006-11-02
AU782958B2 (en) 2005-09-15
US7230157B1 (en) 2007-06-12
EP1242604B1 (de) 2006-05-03
WO2001034821A9 (en) 2002-06-27

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