DE4006351A1 - Maintaining internal pressure in liq. chromatography column - and countering shrinkage by externally adjustable piston - Google Patents

Maintaining internal pressure in liq. chromatography column - and countering shrinkage by externally adjustable piston

Info

Publication number
DE4006351A1
DE4006351A1 DE19904006351 DE4006351A DE4006351A1 DE 4006351 A1 DE4006351 A1 DE 4006351A1 DE 19904006351 DE19904006351 DE 19904006351 DE 4006351 A DE4006351 A DE 4006351A DE 4006351 A1 DE4006351 A1 DE 4006351A1
Authority
DE
Germany
Prior art keywords
piston
adjustable
column
liq
chromatography column
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
DE19904006351
Other languages
German (de)
Inventor
Edgar Grom
Wolfgang Schumacher
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to DE19904006351 priority Critical patent/DE4006351A1/en
Publication of DE4006351A1 publication Critical patent/DE4006351A1/en
Withdrawn legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/22—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6004—Construction of the column end pieces
    • G01N30/6021—Adjustable pistons
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G01N30/52—Physical parameters
    • G01N2030/522—Physical parameters pressure
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6004—Construction of the column end pieces

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Abstract

The tendency of high pressure liq. chromatography column solids to shrink after a period, leaving gaps with a deleterious effect on subsequent sepn., is countered by application from the exterior of piston pressures which prevent dead vol. formation. Modified methods of applying such pressure include an adjustable piston applied to the piston by a threaded cap or flange. The piston seal is radially compressible from the exterior. The piston head can be applied to existing HPLC columns. ADVANTAGE - The columns may be initially completely filled and their service life is extended.

Description

HPLC-Säulen sind Teil eines Hockdruckflüssigkeitschromatographen und werden zur Auftrennung fast aller löslichen Substanzen verwendet. Ein Nachteil der herkömmlichen Säulen ist, daß nach einiger Zeit die Säulenpackung schwindet. Daraus resultiert Totvolumen, welches eine weitere Trennung verschlechtert bzw. unmöglich macht. Eine herkömmliche HPLC-Säule kann außerdem nicht trocken gepackt werden, da sich das Bett stets setzen wird.HPLC columns are part of a high pressure liquid chromatograph and are used to separate almost all soluble substances. A disadvantage of the conventional columns is that after some time the Column pack disappears. This results in dead volume, which further separation deteriorates or makes impossible. A conventional HPLC column can also not be packed dry, because the bed will always sit down.

Der Erfindung liegt die Aufgabe zugrunde, Totvolumen in HPLC-Säulen zu vermeiden.The invention has for its object dead volumes in HPLC columns to avoid.

Diese Aufgabe wird dadurch gelöst, daß ein von außen nachführbarer Kolben Totvolumen verhindert. Um Dichtigkeitsprobleme zu verhindern, ist die Kolbendichtung, von außen, radial nachspannbar.This object is achieved in that an externally trackable Piston dead volume prevented. To prevent tightness problems, the piston seal is radially retensionable from the outside.

Die Befestigung von Säulenkopf mit Kolben erfolgt auf 3 Arten:There are 3 ways to attach the column head to the piston:

  • 1. Kopf mit Gewinde an HPLC-Säule geschraubt.1. Threaded head screwed to HPLC column.
  • 2. Auf der HPLC-Säule befindet sich ein Befestigungsgewinde.2. There is a fastening thread on the HPLC column.
  • 3. Kopf wird mittels Flansch angebracht.3. Head is attached using a flange.

Die Vorteile der Erfindung bestehen in einer langen Lebensdauer der Säulen, in stets optimalen Trennungen sowie der Möglichkeit, Säulen trocken zu füllen.The advantages of the invention are a long service life of the columns, in always optimal separations as well as the possibility Fill columns dry.

AusführungsbeispieleEmbodiments

Fig. 1: HPLC-Säule mit verstellbarem Kolben. Fig. 1: HPLC column with adjustable flask.

Fig. 2: HPLC-Säule mit verstellbarem Kolben mit Befestigungsgewinde an der Säule. Fig. 2: HPLC column with adjustable piston with fastening thread on the column.

Fig. 3: HPLC-Säule mit verstellbarem Kolben, Kopf geflanscht. Fig. 3: HPLC column with adjustable piston, flanged head.

Claims (6)

1. HPLC-Säule mit verstellbarem Kolben, dadurch gekennzeichnet, daß der Kolben von außen axial verstellbar ist.1. HPLC column with adjustable piston, characterized in that the piston is axially adjustable from the outside. 2. HPLC-Säule mit verstellbarem Kolben, dadurch gekennzeichnet, daß die Kolbendichtung von außen radial verstellbar ist.2. HPLC column with adjustable piston, characterized in that the piston seal is radially adjustable from the outside. 3. HPLC-Säule mit verstellbarem Kolben, dadurch gekennzeichnet, daß der Kopf mit Kolben direkt an vorhandenen HPLC-Säulen angebracht wird.3. HPLC column with adjustable piston, characterized in that the head with piston attached directly to existing HPLC columns becomes. 4. HPLC-Säule mit verstellbarem Kolben, dadurch gekennzeichnet, daß der Kopf mittels Befestigungsgewinde an der Säule angebracht wird.4. HPLC column with adjustable flask, characterized in that the head is attached to the column by means of fastening threads becomes. 5. HPLC-Säule mit verstellbarem Kolben, dadurch gekennzeichnet, daß Säulenkopf und Kolben mittels Flansch angebracht sind.5. HPLC column with adjustable piston, characterized in that The column head and piston are attached by means of a flange. 6. HPLC-Säule mit verstellbarem Kolben, dadurch gekennzeichnet, daß die Anschlußkapillaren direkt an der Säulenfritte endet. Es befindet sich deshalb zwischen Ferrule und Anpreßschraube ein Distanzstück.6. HPLC column with adjustable piston, characterized in that the connecting capillaries end directly at the column frit. It is therefore between the ferrule and the pressure screw a spacer.
DE19904006351 1990-03-01 1990-03-01 Maintaining internal pressure in liq. chromatography column - and countering shrinkage by externally adjustable piston Withdrawn DE4006351A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE19904006351 DE4006351A1 (en) 1990-03-01 1990-03-01 Maintaining internal pressure in liq. chromatography column - and countering shrinkage by externally adjustable piston

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19904006351 DE4006351A1 (en) 1990-03-01 1990-03-01 Maintaining internal pressure in liq. chromatography column - and countering shrinkage by externally adjustable piston

Publications (1)

Publication Number Publication Date
DE4006351A1 true DE4006351A1 (en) 1991-09-05

Family

ID=6401154

Family Applications (1)

Application Number Title Priority Date Filing Date
DE19904006351 Withdrawn DE4006351A1 (en) 1990-03-01 1990-03-01 Maintaining internal pressure in liq. chromatography column - and countering shrinkage by externally adjustable piston

Country Status (1)

Country Link
DE (1) DE4006351A1 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007050416A2 (en) * 2005-10-25 2007-05-03 Phenomenex, Inc. Method and apparatus for packing chromatography columns
WO2015043830A1 (en) * 2013-09-27 2015-04-02 Ge Healthcare Bio-Sciences Ab Chromatography column assembly

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007050416A2 (en) * 2005-10-25 2007-05-03 Phenomenex, Inc. Method and apparatus for packing chromatography columns
WO2007050416A3 (en) * 2005-10-25 2007-08-09 Phenomenex Inc Method and apparatus for packing chromatography columns
JP2009513976A (en) * 2005-10-25 2009-04-02 フェノメネクス インコーポレイテッド Chromatography column packing method and apparatus
US7674383B2 (en) 2005-10-25 2010-03-09 Phenomenex, Inc. Method and apparatus for packing chromatography columns
US10603604B2 (en) 2005-10-25 2020-03-31 Phenomenex, Inc. HPLC column
WO2015043830A1 (en) * 2013-09-27 2015-04-02 Ge Healthcare Bio-Sciences Ab Chromatography column assembly
CN105556302A (en) * 2013-09-27 2016-05-04 通用电气健康护理生物科学股份公司 Chromatography column assembly
US10315134B2 (en) 2013-09-27 2019-06-11 Ge Healthcare Bio-Science Ab Chromatography column assembly with snap fit end cap and adaptor shaft
CN110327661A (en) * 2013-09-27 2019-10-15 通用电气健康护理生物科学股份公司 Chromatographic column component

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Legal Events

Date Code Title Description
8110 Request for examination paragraph 44
8139 Disposal/non-payment of the annual fee