DE3752281D1 - Stabilisierung von messenger-rns in tierischen zellen - Google Patents
Stabilisierung von messenger-rns in tierischen zellenInfo
- Publication number
- DE3752281D1 DE3752281D1 DE3752281T DE3752281T DE3752281D1 DE 3752281 D1 DE3752281 D1 DE 3752281D1 DE 3752281 T DE3752281 T DE 3752281T DE 3752281 T DE3752281 T DE 3752281T DE 3752281 D1 DE3752281 D1 DE 3752281D1
- Authority
- DE
- Germany
- Prior art keywords
- interest
- untranslated region
- dna
- poly
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21069—Protein C activated (3.4.21.69)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/108—Plasmid DNA episomal vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
- C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/30—Vector systems having a special element relevant for transcription being an enhancer not forming part of the promoter region
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US90706786A | 1986-09-12 | 1986-09-12 | |
PCT/US1987/001964 WO1988002029A1 (en) | 1986-09-12 | 1987-09-03 | Messenger rna stabilization in animal cells |
Publications (2)
Publication Number | Publication Date |
---|---|
DE3752281D1 true DE3752281D1 (de) | 1999-07-15 |
DE3752281T2 DE3752281T2 (de) | 2000-01-13 |
Family
ID=25423461
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE3752281T Expired - Lifetime DE3752281T2 (de) | 1986-09-12 | 1987-09-03 | Stabilisierung von messenger-rns in tierischen zellen |
Country Status (8)
Country | Link |
---|---|
EP (1) | EP0282512B1 (de) |
JP (2) | JP2619891B2 (de) |
AT (1) | ATE181115T1 (de) |
AU (1) | AU601214B2 (de) |
CA (1) | CA1297435C (de) |
DE (1) | DE3752281T2 (de) |
DK (1) | DK256788A (de) |
WO (1) | WO1988002029A1 (de) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9027917D0 (en) * | 1990-12-21 | 1991-02-13 | Ici Plc | Expression systems |
US7557197B2 (en) * | 2004-01-28 | 2009-07-07 | Oregon Health & Science University | Human soluble neuropilin-1 primary polyadenylation signal and uses thereof |
JP5240699B2 (ja) * | 2006-03-29 | 2013-07-17 | 独立行政法人農業生物資源研究所 | 効率的なトランスジェニックカイコの作出方法 |
US9796973B2 (en) | 2011-04-28 | 2017-10-24 | Yamaguchi University | Terminator sequence-containing reverse primer for overexpression and linear DNA |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4624926A (en) * | 1981-01-02 | 1986-11-25 | The Research Foundation Of State University Of New York | Novel cloning vehicles for polypeptide expression in microbial hosts |
JPS58146281A (ja) * | 1981-10-19 | 1983-08-31 | Suntory Ltd | 酵母細胞の形質転換法 |
US4663281A (en) * | 1984-03-22 | 1987-05-05 | Mass Institute Of Technology | Enhanced production of proteinaceous materials in eucaryotic cells |
EP0173552B1 (de) * | 1984-08-24 | 1991-10-09 | The Upjohn Company | Rekombinante DNA-Verbindungen und Expression von Polypeptiden wie tPA |
US4666848A (en) * | 1984-08-31 | 1987-05-19 | Cetus Corporation | Polypeptide expression using a portable temperature sensitive control cassette with a positive retroregulatory element |
US4675285A (en) * | 1984-09-19 | 1987-06-23 | Genetics Institute, Inc. | Method for identification and isolation of DNA encoding a desired protein |
JP2549504B2 (ja) * | 1984-12-21 | 1996-10-30 | ア−ス製薬株式会社 | Dna塩基配列、ポリペプチド分泌発現ベクター及び形質転換微生物 |
WO1986005807A1 (en) * | 1985-04-01 | 1986-10-09 | Celltech Limited | Transformed myeloma cell-line and a process for the expression of a gene coding for a eukaryotic polypeptide employing same |
FR2592449B1 (fr) * | 1985-12-30 | 1989-12-08 | Aerospatiale | Amortisseur de rotation |
AU582288B2 (en) * | 1986-03-07 | 1989-03-16 | Damon Biotech Inc. | Vector and method for achieving high level expression in eukaryotic cells |
-
1987
- 1987-08-25 CA CA000545276A patent/CA1297435C/en not_active Expired - Fee Related
- 1987-09-03 DE DE3752281T patent/DE3752281T2/de not_active Expired - Lifetime
- 1987-09-03 AT AT87905533T patent/ATE181115T1/de not_active IP Right Cessation
- 1987-09-03 AU AU78510/87A patent/AU601214B2/en not_active Ceased
- 1987-09-03 WO PCT/US1987/001964 patent/WO1988002029A1/en active IP Right Grant
- 1987-09-03 JP JP62505072A patent/JP2619891B2/ja not_active Expired - Lifetime
- 1987-09-03 EP EP87905533A patent/EP0282512B1/de not_active Expired - Lifetime
-
1988
- 1988-05-10 DK DK256788A patent/DK256788A/da not_active Application Discontinuation
-
1994
- 1994-05-09 JP JP6094831A patent/JP2622355B2/ja not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
EP0282512A1 (de) | 1988-09-21 |
EP0282512A4 (de) | 1990-04-10 |
JP2619891B2 (ja) | 1997-06-11 |
AU7851087A (en) | 1988-04-07 |
DK256788D0 (da) | 1988-05-10 |
EP0282512B1 (de) | 1999-06-09 |
JPH02500947A (ja) | 1990-04-05 |
WO1988002029A1 (en) | 1988-03-24 |
CA1297435C (en) | 1992-03-17 |
DK256788A (da) | 1988-05-10 |
JP2622355B2 (ja) | 1997-06-18 |
DE3752281T2 (de) | 2000-01-13 |
AU601214B2 (en) | 1990-09-06 |
ATE181115T1 (de) | 1999-06-15 |
JPH07163388A (ja) | 1995-06-27 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition |