DE3709101A1 - IMMOBILIZATION SUBSTANCE - Google Patents

IMMOBILIZATION SUBSTANCE

Info

Publication number
DE3709101A1
DE3709101A1 DE19873709101 DE3709101A DE3709101A1 DE 3709101 A1 DE3709101 A1 DE 3709101A1 DE 19873709101 DE19873709101 DE 19873709101 DE 3709101 A DE3709101 A DE 3709101A DE 3709101 A1 DE3709101 A1 DE 3709101A1
Authority
DE
Germany
Prior art keywords
substance
immobilizing
substance according
cells
tissues
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
DE19873709101
Other languages
German (de)
Inventor
Wolfgang Geor Mueller-Lierheim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ANAWA MUENCHEN AG BIOLOGISCHE LABORATORIEN, 8033 P
Original Assignee
MUELLER LIERHEIM KG BIOLOG LAB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by MUELLER LIERHEIM KG BIOLOG LAB filed Critical MUELLER LIERHEIM KG BIOLOG LAB
Priority to DE19873709101 priority Critical patent/DE3709101A1/en
Priority to PCT/DE1988/000165 priority patent/WO1988007078A1/en
Priority to EP88902404A priority patent/EP0349558A1/en
Publication of DE3709101A1 publication Critical patent/DE3709101A1/en
Withdrawn legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
    • A61L24/04—Surgical adhesives or cements; Adhesives for colostomy devices containing macromolecular materials
    • A61L24/10—Polypeptides; Proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
    • A61L24/04—Surgical adhesives or cements; Adhesives for colostomy devices containing macromolecular materials
    • A61L24/10—Polypeptides; Proteins
    • A61L24/106—Fibrin; Fibrinogen
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier

Abstract

An immobilization substance, characterized by adhesive proteins (adhesins), isolated from extracellular structures, in particular fimbria, pili, external membranes or capsules of Gram-negative bacteria, in particular coli bacteria, for binding cells, in particular mammal cells, tissues, pharmaceutical active principles, antibodies and growth factors to carriers.

Description

Die Aufgabe der Erfindung besteht darin, eine Immobilisie­ rungssubstanz zur Bindung von biologischem Material an Trä­ gern zu schaffen.The object of the invention is to immobilize rungssubstanz for binding biological material to Trä like to create.

Diese Immobilisierungssubstanz ist dadurch gekennzeichnet, daß adhäsive Proteine, die aus extrazellulären Strukturen, insbesondere Fimbrien, Pili, äußere Membranen oder Kapseln von Gram-negativen Bakterien, insbesondere Colibakterien, isoliert sind, verwendet werden.This immobilizing substance is characterized in that that adhesive proteins that come from extracellular structures, especially fimbria, pili, outer membranes or capsules Gram-negative bacteria, especially coli bacteria, are isolated, are used.

Diese adhäsiven Proteine besitzen typischerweise ein Moleku­ largewicht von 20 K und können Kohlehydratkomponenten von Glycolipoiden und Glycoproteinen höherer Zellen erkennen. Diese adhäsiven Proteine vermitteln die Adhärenz der Bakte­ rien an höhere Zellen (z.B. Erythrozyten, Lymphozyten) und an Gewebe (z.B. Epithel). Diese adhäsiven Proteine werden im allgemeinen von Säugetier-Proteosen nicht gespalten.These adhesive proteins typically have one molecule lar weight of 20 K and can carbohydrate components from Recognize glycolipoids and higher cell glycoproteins. These adhesive proteins mediate the adherence of the bacteria adhere to higher cells (e.g. erythrocytes, lymphocytes) and Tissue (e.g. epithelium). These adhesive proteins are in the generally not cleaved by mammalian proteoses.

Diese isolierten adhäsiven Proteine lassen sich zur Immobili­ sierung von Zellen, insbesondere Säugerzellen, Geweben, von pharmazeutischen Wirkstoffen, Antikörpern oder Wachstumskör­ pern an Trägern verwenden. Insbesondere für die Oberflächen­ aufbereitung von Implantaten lassen sich diese adhäsiven Proteine als Anheftfaktoren für Säugetierzellen bzw. Gewebe verwenden. Die Zellen bzw. Gewebe lassen sich über die ad­ häsiven Proteine an der Oberfläche eines Implantatgrundkör­ pers binden. Als Wachstumsfaktoren kommen Blutserum oder Be­ standteile davon sowie Fibronectin in Frage. Auch eine Kombi­ nation aus Stoffwechselprodukten von Zellen und auf biochemi­ schem Weg modifizierten Naturprodukten sind als Wachstumsfak­ toren geeignet. Weitere Wachstumsfaktoren können zur Zelldif­ ferenzierung führende Proteine Knochensubstanz induzierende Proteine sein. Bei den Implantaten kann es sich um extrakor­ porale oder implantierbare künstliche Organe, Gefäße, Pro­ thesen, Hautersatz, Zahnersatz, künstliche Augenlinsen, Kon­ taktlinsen und dgl. handeln.These isolated adhesive proteins can be immobilized  sation of cells, especially mammalian cells, tissues, of active pharmaceutical ingredients, antibodies or growth bodies Use core on straps. Especially for the surfaces preparation of implants, these can be adhesive Proteins as attachment factors for mammalian cells or tissues use. The cells or tissues can be accessed via the ad adhesive proteins on the surface of an implant base body bind pers. Blood serum or Be come as growth factors components of it as well as fibronectin in question. Also a station wagon nation from metabolites of cells and on biochemi modified natural products are a growth factor suitable for gates. Further growth factors can contribute to cell dif proteins leading to bone induction Be proteins. The implants can be extracorrect poral or implantable artificial organs, vessels, pro theses, skin replacement, dentures, artificial eye lenses, con act tactical lenses and the like.

Auch bei der Formulierung von Arzneimitteln lassen sich die adhäsiven Proteine zur Bindung der phamazeutischen Wirkstoffe insbesondere von Blutgerinnungsmitteln, an den Trägerkörpern, insbesondere pulverförmigen Trägerkörpern, verwenden.They can also be used in the formulation of pharmaceuticals adhesive proteins for binding the pharmaceutical active ingredients in particular of blood coagulants on the carrier bodies, in particular use powdered carrier bodies.

Als Trägerkörper kommen auch Kohlenhydratkomponenten von Gly­ colipoiden und Glycoproteinen enthaltende höhere Zellen in Frage, da diese von den adhäsiven Proteinen spezifisch er­ kannt werden.Carbohydrate components from Gly also come as support bodies higher cells containing colipoids and glycoproteins in  Question as this specific of the adhesive proteins he be known.

Ferner können die adhäsiven Proteine als Immobilisierungssub­ stanz für Antikörper produzierende Zellen, insbesondere Säu­ gerzellen, von Gewebe und dgl. verwendet werden.Furthermore, the adhesive proteins can be used as immobilization sub punch for antibody-producing cells, especially acid ger cells, tissue and the like.

Claims (7)

1. Immobilisierungssubstanz gekennzeichnet durch adhäsive Proteine, die aus extrazellulären Strukturen, insbesondere Fimbrien, Pili, äußeren Membranen oder Kapseln von Gram-nega­ tiven Bakterien, insbesondere Colibakterien, isoliert sind, zur Bindung von Zellen, insbesondere Säugerzellen, Geweben, pharmazeutischen Wirkstoffen, Antikörpern, Wachstumsfaktoren an Trägern.1. immobilizing substance characterized by adhesive proteins which are isolated from extracellular structures, in particular fimbriae, pili, outer membranes or capsules of Gram-negative bacteria, in particular coli bacteria, for binding cells, in particular mammalian cells, tissues, pharmaceutical active substances, antibodies, Growth factors on carriers. 2. Immobilisierungssubstanz nach Anspruch 1, dadurch gekennzeichnet, daß durch die Immobilisierungs­ substanz spezifisch Kohlenhydratkomponenten von Glycolipoiden oder Glycoproteinen erkannt bzw. adsorptiv gebunden werden. 2. Immobilizing substance according to claim 1, characterized characterized by the immobilization Substance specific carbohydrate components of glycolipoids or glycoproteins are recognized or adsorptively bound.   3. Immobilisierungssubstanz nach Anspruch 1 oder 2, da­ durch gekennzeichnet, daß es sich bei den erkannten bzw. gebundenen Kohlenhydratkomponenten um solche von in der äußeren Membran von Säugetierzellen vorkommende Glycolipoide oder Glycoproteine handelt.3. Immobilizing substance according to claim 1 or 2, because characterized in that it is the recognized or bound carbohydrate components around such of those found in the outer membrane of mammalian cells Glycolipoids or glycoproteins. 4. Immobilisierungssubstanz nach den Ansprüchen 1 bis 3, dadurch gekennzeichnet, daß die Bindung der Immobilisierungssubstanz an säugetierzell-membranständige Glycolipoide oder Glycoproteine artspezifisch erfolgt.4. immobilizing substance according to claims 1 to 3, characterized in that the bond the immobilization substance on mammalian cell membrane Glycolipoide or Glycoproteine species-specific. 5. Immobilisierungssubstanz nach den Ansprüchen 1 bis 4, dadurch gekennzeichnet, daß die Substanz zur Immobilisierung von Säugetierzellen und -geweben, insbe­ sondere menschliche Zellen und Gewebe an Implantate oder künstliche Organe verwendet wird.5. immobilizing substance according to claims 1 to 4, characterized in that the substance for the immobilization of mammalian cells and tissues, esp special human cells and tissues on implants or artificial organs is used. 6. Immobilisierungssubstanz nach den Ansprüchen 1 bis 4, dadurch gekennzeichnet, daß die Substanz zur Formulierung oder als Wirkstoff von Arzneimitteln oder zur Herstellung von Diagnostika Verwendung findet.6. immobilizing substance according to claims 1 to 4, characterized in that the substance for formulation or as an active ingredient in medicinal products or used for the production of diagnostics. 7. Immobilisierungssubstanz nach den Ansprüchen 1 bis 4, dadurch gekennzeichnet, daß die Substanz zur Zellkultur oder Zelltrennung in vitro Verwendung findet.7. immobilizing substance according to claims 1 to 4, characterized in that the substance used for cell culture or cell separation in vitro.
DE19873709101 1987-03-20 1987-03-20 IMMOBILIZATION SUBSTANCE Withdrawn DE3709101A1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
DE19873709101 DE3709101A1 (en) 1987-03-20 1987-03-20 IMMOBILIZATION SUBSTANCE
PCT/DE1988/000165 WO1988007078A1 (en) 1987-03-20 1988-03-17 Immobilization substance
EP88902404A EP0349558A1 (en) 1987-03-20 1988-03-17 Immobilization substance

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19873709101 DE3709101A1 (en) 1987-03-20 1987-03-20 IMMOBILIZATION SUBSTANCE

Publications (1)

Publication Number Publication Date
DE3709101A1 true DE3709101A1 (en) 1988-09-29

Family

ID=6323543

Family Applications (1)

Application Number Title Priority Date Filing Date
DE19873709101 Withdrawn DE3709101A1 (en) 1987-03-20 1987-03-20 IMMOBILIZATION SUBSTANCE

Country Status (3)

Country Link
EP (1) EP0349558A1 (en)
DE (1) DE3709101A1 (en)
WO (1) WO1988007078A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1027893A2 (en) * 1991-05-31 2000-08-16 Gliatech, Inc. Alginate containing medicament and implant for treating fibrosis

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8826116D0 (en) 1988-11-08 1988-12-14 Danbiosyst Ltd Adhesive drug delivery composition
SE8804164A0 (en) * 1988-11-17 1990-05-18 Per Prisell Pharmaceutical preparation
DE3936568C2 (en) * 1989-11-03 1997-06-19 Karlheinz Prof Dr Dr Schmidt Active ingredient complex for the production of biological parts in the form of organs for living things; Method of making the same and its use
IT1255657B (en) * 1992-05-29 1995-11-09 DIFFERENTIATED CONJUNCTIVAL MUCOSA FABRIC CULTURES, PROCEDURES FOR THEIR PREPARATION AND SUPPORTS FOR THEIR USE.
US7214654B1 (en) 1994-12-07 2007-05-08 Karlheinz Schmidt Agent for the manufacture of biological parts including an active ingredient complex and carrying materials suitable for the active ingredient complex
US5928635A (en) * 1994-12-07 1999-07-27 Schmidt; Karlheinz Process for producing active agent complexes
US5916553A (en) * 1992-09-17 1999-06-29 Schmidt; Karlheinz Complex for inducing bone growth in the mastoid cavity

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1027893A2 (en) * 1991-05-31 2000-08-16 Gliatech, Inc. Alginate containing medicament and implant for treating fibrosis
EP1027893A3 (en) * 1991-05-31 2003-01-02 Gliatech, Inc. Alginate containing medicament and implant for treating fibrosis

Also Published As

Publication number Publication date
WO1988007078A1 (en) 1988-09-22
EP0349558A1 (en) 1990-01-10

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Legal Events

Date Code Title Description
8127 New person/name/address of the applicant

Owner name: ANAWA MUENCHEN AG BIOLOGISCHE LABORATORIEN, 8033 P

8141 Disposal/no request for examination