DE10144291C2 - Device for isolating biological material bound to magnetic particles in a magnetic field - Google Patents
Device for isolating biological material bound to magnetic particles in a magnetic fieldInfo
- Publication number
- DE10144291C2 DE10144291C2 DE2001144291 DE10144291A DE10144291C2 DE 10144291 C2 DE10144291 C2 DE 10144291C2 DE 2001144291 DE2001144291 DE 2001144291 DE 10144291 A DE10144291 A DE 10144291A DE 10144291 C2 DE10144291 C2 DE 10144291C2
- Authority
- DE
- Germany
- Prior art keywords
- magnetic field
- biological material
- chamber
- magnetic
- material bound
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/02—Magnetic separation acting directly on the substance being separated
- B03C1/025—High gradient magnetic separators
- B03C1/031—Component parts; Auxiliary operations
- B03C1/032—Matrix cleaning systems
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/02—Magnetic separation acting directly on the substance being separated
- B03C1/28—Magnetic plugs and dipsticks
- B03C1/288—Magnetic plugs and dipsticks disposed at the outer circumference of a recipient
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C2201/00—Details of magnetic or electrostatic separation
- B03C2201/26—Details of magnetic or electrostatic separation for use in medical applications
Description
Die Erfindung betrifft eine Vorrichtung zur Isolierung von an magnetischen Partikeln gebundenem biologischen Ma terial in einem Magnetfeld, die mit einer Kammer und ei nem Magneten versehen ist. The invention relates to a device for insulation of biological matter bound to magnetic particles material in a magnetic field with a chamber and egg magnet is provided.
Eine derartige Vorrichtung ist aus der DE 37 20 844 C2 bekannt. Bei dieser Vorrichtung ist in der Kammer eine magnetische Struktur vorgesehen, an der das an magneti schen Partikeln gebundene biologische Material anhaften soll. Bei dieser Vorrichtung ist das "Ernten" des iso lierten biologischen Materials schwierig. Aus der DE 39 25 093 C2 ist eine weitere Vorrichtung der eingangs ge nannten Art bekannt, bei der die magnetischen Kraftlinien bereits senkrecht zur Bewegungsrichtung der biologischen Partikel in der Trennkammer verlaufen. Weitere Ausgestal tungen der gattungsgemäße Vorrichtung zeigen die WO 99/626222 A1 und die WO 93/20927 A1, auch bei diesen Ausge staltungen ist die erreichbare Spezifität für bestimmte Fragestellungen nicht ausreichend.Such a device is from DE 37 20 844 C2 known. In this device there is one in the chamber provided magnetic structure on which the magneti attached biological material should. In this device, the "harvesting" of the iso biological material difficult. From DE 39 25 093 C2 is another device of the beginning ge known type, in which the magnetic lines of force already perpendicular to the direction of movement of the biological Particles run in the separation chamber. Further design Solutions of the generic device show WO 99/626222 A1 and WO 93/20927 A1, also in these Ausge is the achievable specificity for certain Questions not sufficient.
Eine hohe Spezifität, wie sie erforderlich ist, um klein ste, subzelluläre Teilchen zu selektieren, setzt das Vor handensein eines hohen Magnetfelds voraus. Der Erfindung liegt die Aufgabe zugrunde, eine Vorrichtung zu schaffen, die es ermöglicht, an magnetische Partikeln gebundenes biologisches Material mit einer hohen Ausbeute und hohem Reinheitsgrad zu gewinnen.A high specificity, as required to be small The prerequisite is to select subcellular particles presence of a high magnetic field. The invention is based on the task of creating a device which allows it to be bound to magnetic particles biological material with a high yield and high Gain purity.
Erfindungsgemäß wird diese Aufgabe durch die Merkmale des Anspruchs 1 gelöst.According to the invention, this object is achieved through the features of Claim 1 solved.
Eine bevorzugte Ausgestaltung zeichnet sich aus durch ein einen Spülstrom in der Kammer bewirkendes Pumpsystem.A preferred embodiment is characterized by pumping system causing a purge flow in the chamber.
Der Erfindung liegt die Erkenntnis zugrunde, daß bei ei nem sehr hoch fokussierten Magnetfeld, in dessen Nähe die magnetisierten Partikel durch Konvektion vorbeigeführt werden, diese Partikel im Bereich des Magnetfeldes fixiert werden. The invention is based on the finding that in egg a very highly focused magnetic field, in the vicinity of which magnetized particles passed by convection these particles in the area of the magnetic field be fixed.
Die Erfindung wird anhand einer Zeichnung erläutert. Dabei zeigt:The invention is explained with reference to a drawing. It shows:
Fig. 1a das der Erfindung zugrundeliegende Prinzip, FIG. 1a, the object underlying the invention principle,
Fig. 1b den Spülvorgang, FIG. 1b, the flushing operation,
Fig. 2 eine Draufsicht auf die Vorrichtung und Fig. 2 is a plan view of the device and
Fig. 3 eine Schnittdarstellung durch die Vorrichtung. Fig. 3 is a sectional view through the device.
Die Vorrichtung besteht aus einem Rahmen 14, der die Kam mer 10 bildet. In dem Rahmen sind mehrere Zuflüsse (durch nach innen zeigende Pfeile markiert) und Abflüsse (durch nach außen weisende Pfeile markiert) vorgesehen.The device consists of a frame 14 which forms the cam mer 10 . Several inflows (marked by arrows pointing inwards) and outflows (marked by arrows pointing outwards) are provided in the frame.
Die eine Rahmenfläche ist durch eine dünne Membran 16, die andere Rahmenfläche durch eine Wandung 18 - vorzugs weise eine andere dünne Membran oder ein Glas - abge deckt.One frame surface is covered by a thin membrane 16 , the other frame surface by a wall 18 - preferably another thin membrane or a glass.
Der Magnet 12 trägt eine Vielzahl von parallel zueinander verlaufenden Eisen-Halbstäben 17 (in der Zeichnung sind nur drei dargestellt, tatsächlich aber sind weit mehr Eisen-Halbstäbe 17 vorgesehen). Diese Eisen-Halbstäbe 17, die einen Durchmesser von etwa 0,5 mm, vorzugweise 0,3 mm haben, liegen an der dünnen Membran 16 unmittelbar an. Die Eisen-Halbstäbe 17 dienen dazu, das Magnetfeld zu fo kussieren, das Magnetfeld ist also im Bereich ihres Kon takts zu der dünnen Membran 16 besonders groß. The magnet 12 carries a plurality of iron half-bars 17 running parallel to one another (only three are shown in the drawing, but in fact far more iron half-bars 17 are provided). These iron half-bars 17 , which have a diameter of approximately 0.5 mm, preferably 0.3 mm, rest directly on the thin membrane 16 . The iron half-bars 17 are used to kiss the magnetic field fo, so the magnetic field is particularly large in the area of their contact with the thin membrane 16 .
In der Kammer 10 entsteht bei Bestehen einer Temperatur differenz zwischen der Membran 16 und der Wandung 18 eine Konvektion (das Magnetsystem 12, 17 mit der Membran 16 werden konstand neha des Schmelzpunktes (4°C) gehalten, während die gegenüberliegende Wandung 18 nahe einer Kühl schranktemperatur (nahe 8°C) gehalten wird, wodurch der Temperarurgradient durch die thermische Leitfähigkeit durch die Rahmenflächen als auch durch das Fluid in der Kammer 10 erhalten wird). Auf diese Weise wird das in die Kammer eingebrachte biologische Material zirkulieren, das an magnetische Partikel gebundene biologische Material setzt sich auf der dünnen Membran 16 dort, wo diese auf der gegenüberliegenden Seite mit den Eisen-Halbstäben 17 in Kontakt ist, ab. Nach Auswaschen des nicht an magneti sche Partikel gebundenen biologischen Materials aus der Kammer - das an magnetische Partikel gebundene biologi sche Material bleibt von dem Magnetfeld in dem Bereich der Eisen-Halbstäbe haften - verbleibt das zu isolierende biologische Material in der Kammer und kann - etwa durch Pipettieren - gewonnen werden.In the chamber 10 , when there is a temperature difference between the membrane 16 and the wall 18, convection occurs (the magnet system 12 , 17 with the membrane 16 are held close to the melting point (4 ° C.), while the opposite wall 18 is close to cooling cabinet temperature (close to 8 ° C) is maintained, whereby the temperature gradient is obtained through the thermal conductivity through the frame surfaces as well as through the fluid in the chamber 10 ). In this way, the biological material introduced into the chamber will circulate, and the biological material bound to magnetic particles will settle on the thin membrane 16 where it contacts the iron half-rods 17 on the opposite side. After washing out the biological material not bound to magnetic particles from the chamber - the biological material bound to magnetic particles remains adhering to the magnetic field in the area of the iron half-bars - the biological material to be isolated remains in the chamber and can - for example through Pipetting - to be won.
Claims (2)
die Kammer (10) durch einen Rahmen (14) definiert ist, dessen eine Fläche durch eine dünne Membran (16) und dessen andere Fläche durch eine Wandung (18) abge deckt ist,
das Magnetsystem (12, 17) eine Vielzahl von paral lel zueinander verlaufenden, das Magnetfeld fokussie renden Eisen-Halbstäben (17) aufweist, die an der dün nen Membran (16) anliegen, und
wobei die Membran (16) und die Wandung (18) unter Aus bildung einer Konvektion des Fluids in der Kammer (10) unterschiedliche Temperaturen annehmen können.1. Device for isolating biological material bound to magnetic particles in a magnetic field, with a chamber ( 10 ) and a magnet system ( 12 , 17 ), characterized in that
the chamber ( 10 ) is defined by a frame ( 14 ), one surface of which is covered by a thin membrane ( 16 ) and the other surface of which is covered by a wall ( 18 ),
the magnet system ( 12 , 17 ) has a plurality of parallel to one another, the magnetic field focussing iron half-bars ( 17 ) which abut the thin membrane ( 16 ), and
whereby the membrane ( 16 ) and the wall ( 18 ) can form different temperatures from the formation of a convection of the fluid in the chamber ( 10 ).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE2001144291 DE10144291C2 (en) | 2001-09-08 | 2001-09-08 | Device for isolating biological material bound to magnetic particles in a magnetic field |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE2001144291 DE10144291C2 (en) | 2001-09-08 | 2001-09-08 | Device for isolating biological material bound to magnetic particles in a magnetic field |
Publications (2)
Publication Number | Publication Date |
---|---|
DE10144291A1 DE10144291A1 (en) | 2003-03-27 |
DE10144291C2 true DE10144291C2 (en) | 2003-10-09 |
Family
ID=7698332
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE2001144291 Expired - Fee Related DE10144291C2 (en) | 2001-09-08 | 2001-09-08 | Device for isolating biological material bound to magnetic particles in a magnetic field |
Country Status (1)
Country | Link |
---|---|
DE (1) | DE10144291C2 (en) |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4440638A (en) * | 1982-02-16 | 1984-04-03 | U.T. Board Of Regents | Surface field-effect device for manipulation of charged species |
DE3720844C2 (en) * | 1987-06-24 | 1991-01-10 | Stefan 5060 Bergisch Gladbach De Miltenyi | |
WO1993020927A1 (en) * | 1992-04-16 | 1993-10-28 | British Technology Group Ltd. | Apparatus for separating a mixture |
DE3925093C2 (en) * | 1989-07-28 | 1995-03-30 | Roland Dipl Phys Hartig | Device for separating biological material bound to magnetic particles (beads) in a magnetic field |
WO1999062622A1 (en) * | 1998-05-29 | 1999-12-09 | Industrial Research Limited | Method and apparatus for concentrating and/or positioning particles or cells |
-
2001
- 2001-09-08 DE DE2001144291 patent/DE10144291C2/en not_active Expired - Fee Related
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4440638A (en) * | 1982-02-16 | 1984-04-03 | U.T. Board Of Regents | Surface field-effect device for manipulation of charged species |
DE3720844C2 (en) * | 1987-06-24 | 1991-01-10 | Stefan 5060 Bergisch Gladbach De Miltenyi | |
DE3925093C2 (en) * | 1989-07-28 | 1995-03-30 | Roland Dipl Phys Hartig | Device for separating biological material bound to magnetic particles (beads) in a magnetic field |
WO1993020927A1 (en) * | 1992-04-16 | 1993-10-28 | British Technology Group Ltd. | Apparatus for separating a mixture |
WO1999062622A1 (en) * | 1998-05-29 | 1999-12-09 | Industrial Research Limited | Method and apparatus for concentrating and/or positioning particles or cells |
Also Published As
Publication number | Publication date |
---|---|
DE10144291A1 (en) | 2003-03-27 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
OP8 | Request for examination as to paragraph 44 patent law | ||
8304 | Grant after examination procedure | ||
8364 | No opposition during term of opposition | ||
R119 | Application deemed withdrawn, or ip right lapsed, due to non-payment of renewal fee | ||
R119 | Application deemed withdrawn, or ip right lapsed, due to non-payment of renewal fee |
Effective date: 20150401 |