DD245568A3 - Apparat fuer die zuechtung von mikroorganismen - Google Patents

Apparat fuer die zuechtung von mikroorganismen Download PDF

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Publication number
DD245568A3
DD245568A3 DD23674682A DD23674682A DD245568A3 DD 245568 A3 DD245568 A3 DD 245568A3 DD 23674682 A DD23674682 A DD 23674682A DD 23674682 A DD23674682 A DD 23674682A DD 245568 A3 DD245568 A3 DD 245568A3
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DD
German Democratic Republic
Prior art keywords
öhomacch
microorganisms
ochobahhhmh
cccp
foam
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DD23674682A
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English (en)
Inventor
Aleksandr J Vinarov
Jaroslav J Skop
Vladimir A Osipov
Valerij A Bykov
Original Assignee
Vnii Biosinteza Belkovykh
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Publication of DD245568A3 publication Critical patent/DD245568A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/06—Tubular
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/58—Reaction vessels connected in series or in parallel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/18—Flow directing inserts
    • C12M27/22—Perforated plates, discs or walls
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)

Abstract

Der Apparat zur Zuechtung (Kultivierung) von Mikroorganismen dient zum Einsatz in der mikrobiologischen Industrie bei der Durchfuehrung der Fermentation auf einem kohlenstoffhaltigen Substrat. Ziel der Erfindung ist eine Erhoehung des Konzentrationsgrades der Mikroorganismen im Schaum. Die genannte Zielstellung wird dadurch erreicht, dass in dem Apparat zur Zuechtung der Mikroorganismen nach dem Urheberschein der UdSSR Nr. 788726 die Vorrichtung zum Sammeln und Ableiten der konzentrierten Biomasse eine Kammer zur Trocknung des Schaums enthaelt, die durch einen zylindrischen Schuss und an ihm mit den grossen Grundflaechen befestigten Kegelstuempfen gebildet wird, die uebereinander mit einem dazwischen liegenden Spalt fuer den Durchschnitt des getrockneten Schaums zum Entschaeumer angeordnet sind. Der Neigungswinkel der Mantellinie des oberen Kegels betraegt 45 bis 60, des unteren Kegels 20 bis 45.

Description

OmiGAHME H30BPETEHKH K ABTOPCKOW
: 03.04.81 : J* 326I48I/28-I3
MKH3 CI2M 1/04
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HK3M0B MsodpeTeHHe othochtch κ MHKpoöHOJionniecKofi προΜΗΐπ-
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CCCP Jfe 788726 oimcaH annapaT απη βηρθπιηβθηηη MHKpoopra-ΗΗ3ΜΟΒ.
annapaT cosepsaiT no KpaiHefi Mepe αβθ aHa-KOHCTpyKmiH BepTHKajiBHHe eMKOCTH, pa3ji;ejieHHHe
Π0 BHCOTe Ha CeiOJHH ΠβρφορΗρΟΒβΗΗΗΜΗ ΠβρβΓΟρΟ,ΙίΚδΜΗ, Β KO τορΗχ Haxo,miTCH njiaBaKxiiafl HacasKa. BepxHHH ceKnan eMKOCTH npe,5Ha3HaqeHa mh. KOHiieHTpHpoBaHiiH cycneH3HH MHKpoopraHH3MOB. AifflapaT cHadaceH naTpyOKaMH jyisi
cpeflH is. aapiapyBxiiero ra3a β ηηκηηθ ceKUHii η
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iffipKyjiflrtHOHHoro Hacoca η naTpyÖKy jyis noÄBOsa ra3a eMKOCTH.
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HeAOCTaTOK οοηοβηογο annapaTa saiüiro^qaeTCH β tom, β cjrmecTBjrBaiieM ycTpotcTBe jum cöopa η BHBOfla κοΗΓξβΗτρπρο-BaHHOt ÖHOMaCCH npOHCXOJiHT HeBOCTaTO^HO 9φφβΚΤΗΒΗ0 ΚΟΗΙΪθΗ-TpnpoBaHHe MHKpoopraHH3MOB β neHe.
IlejiBD ÄonojiHHTejiBHoro E3oöpeTeHHH SBJifieTca noBHnieHHe cTeneHH KOHiieHTpHpoBaHHH öHOMaccH. yKa3aHHas n.eJiB äocthraeTca τβΜ, ^το β annapaTe jya BHpamHBaHHa MHKpoopraHH3-MOB no aBTopcKOMy CBHseTejiBCTBy CCCP $ 788726 ycTpoScTBO jsfls. cöopa η BHBO.ua KOHLceHTpHpoBaHHot ÖHOMaccH cHadaceHO Ka-Mepofi ocyniKH neHH, oöpa3OBaHHot
H ΠρΗΚρβΠΛΒΗΗΗΜΗ Κ ΗβΗ 0OJEbDIHMH OCHOBaHHHMH HHMH KOHycaMH, OOpaHjeHHHMH OCHOBaHHHMH ΒΒβρΧ, H paCHOJIDäCeH-
HHMH oflHH Has spyrHM c oöpa3OBaHHeM Messy hhmh 3a3opa äjih ocymeHHot πθηη κ neHoracHTejno, πρπ stom yroji Haoöpasyromet BepxHero KOHyca cocTaBJiaeT 45-60°, a 20-45°.
Ha φΗΓ. I - oomHt BHH annapaTa äjjh: BHpaniHBaHHH mh-KpoopraHH3MOB, Φηγ.2 - BHÄ CBepxy, Ha φΗΓ.3 - pa3pe3 E-B no ycTpotcTBy sjih cöopa π BHBOjia ÖHOMaCCH·
AnnapaT co,nepsHT no KpatHet Mepe ρ,Ββ
no KOHCTpyicmiH BepTHKajiBHHe κολοηηηθ θμκοοτη I,
no BHCOTe Ha ceKUHH πβρφορΗροΒαΗΗΗΜΗ neperoposKa-2, β KOTopHx Haxo^HTCH iuiaBaiomafl Haca^Ka (Ha φκτ.Ι He H3odpaaceHa). BepxHHH cexwH. 3 Kastaofi θμκοοτη npesHa3-Ha^eHa flJLS ΚΟΗΗ,βΗΤρΗρΟΒαΗΗΗ CycneH3HH MHKpOOpraHH3MOB.
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th η naTpyOKH 7 jyra otboä8 OTpaöoTaHHoro ra3a H3 BepxHeÄ οβκϋΗΗ.
BepxHHe ceKUiiH 3 eMKOcTeÄ I TaHreHLmajiBHo
ΗβΗΗ K yCTpoftCTBy 8 ,HJta CÖOpa H BHBO^a ÖHOMaccH, npH^eM.. oOmHt bhboä ÖHOMaccH H3 annapaTa ocymecTBJweTCH β HanpaBJieHHH οτρβΛΚΗ Α.
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üaTpyöKH 7 ajlh 0TB0,n;a OTpaöoTaHHoro raea H3 Kaawot KOJioHHoi eMKOCTH coejiHHeHH c naTpyOKaMH 5 jyui no^Bo,n;a aapHpyromero ra3a β nocxeKymyio no xo,ny npoiiecca θμκοοτβ Ι. H3 naTpyÖKa 7 nocjiejCHeH no xojiy TexHOJiorH^ecKoro nponecca eMKOCTH npoH3BojtHTCÄ nojia^a oTpaöoTaHHoro ra3a no cTpejnce C Ha ero OHHCTKy.
8 (φΗΓ.3) WUl CÖOpa H BHBOSa ΚΟΗϋβΗΤρΗ-
ÖHOMaccH coÄepaaaT cÖopHHK KOHiteHTpaTa ΙΟ, β ΒβρχΗβίί qacTH κοτοροΓο pa3MemeHH neHoracHTejiB II η pacnojioaceHHyH) cHapyxH Hero coocho KaMepy 12 ocyniKH neHH, oöpa30BaHHyio πηληηαρη^θοκοη' oöeqaHKoii 13 η npHKpenjieHHHMH
λ ι ήί». · '-ο -' * j " r o '
K HeS ÖOJE&fflHWH OCHOBaHHaMH .HByMJI yce^eHHLIMH KOHycaMH 14 H 15, OÖpame HHHMH ΜβΗΒΠΙΗΜΗ OCHOBaHHHMZ ΒΒβρΧ H ΧβΗΗΗΜΗ OÄHH Η3£ ßpyTHM C 3a3OpOM MeJSHJT HHMH ΑΠΗ OCpieHHOS ΠβΗΗ K ΠβΗΟΙΏΟΗΤΘΛΗ) II, ΠρΗ 9T0M yiOJI HaKJIOHa oöpasyromeä BepxHero KOHyca cocTaBJiHeT 45-60°, a ηηκηθγο 20-45°.
BepxHHe ceKHHH 3 kojiohhh I noscoeflHHeHH κ Kawepe 12 ocymKii nenu·
AnnapaT paöoTaeT cjie^ffiDfEiM oöpa30M: B nepgyio no xojiy TexHOJiorH^ecKoro npon;ecca θμκοοτβ ÖOK 5 no^iDT aapHppmHii ΙΏ3, HanpaMep bo3-η ocyie ctbjihiot BHpamHBaHHe MHKpoopraHH3MOB β npncyT-njiaEaiomeH HacajtKH, pa3MemeHH0M β οθκε[ηηχ, oopaso-
BaHHHX nep$OpHpOBaHHHMH ΠβρβΓΟρΟίζΚθΜΗ 2. IIOÄa1^ nHTaTeJEB-
hoS cpe,HH H yrjiepoiccoÄepjBamero cyöcTpaTa npoH3BO,5ST ^epe3 naTpyÖKH 4. KomjeHTpnpoBaHHaa cycneH3Ha: MHKpoopraHH3-MOB H3 BepxHeä ceioiHH 3 noÄaeTca β icaMepy 12 ocymKH neHH,
B KOTOpOÄ npOHOXOÄHT Μ^Π>ΕβΜββ KOHIieHTpHpOBaHHe MHKpO-
opraHH3MOB, BCJieÄCTBHe ocymKH neHH η ee raraeHHH β neHoracHTeJie II /ecjm TpedyeTcn/. KoimeHTpaT otbo^t h3 cc5op-HHKa IO nb CTpejiKe A. OcBeTJieHHyio KyjDbTypajr&Hjno Hepe3 naTpyöoK 6 noj^aEDT κ BcacHBaromeMy naTpyÖKy πηοηηογο Hacoca 9 BTopot ΘΜΚ00ΤΗ I. K 3TOMy ss DiHpKyjiHnHOHHoro Hacoca flonojiHHTejrLHO nojiaiOT cpesy η yrsepoÄCOÄepacamHt cyöcTpaT. Äajiee CMecB HacocoM 9 noicaeTCH β cooTBeTCTByiraipie naTpyöKH Βτοροδ kojiohhoh eMKOCTH.
cpejm bo BTopot θμκοοτη ocymecTBJCieTCH 3a β naTpyöoK 5 ra3a, οτβο,εημογο Hs nepBot eM-. -?epe3 naTpyöoK 7·
Bo BTopot η nocjrejayfoinHX θμκοοτηχ ocymecTBJiHeTCii aHanpoijecc HenpepuBHoro BHpamHBaHHS MHKpoopraHH3MOB. H3 nocjieÄHet no xosy npoiiecca θμκοοτη OTÖHpawT naTpyöOK 6 ocBeTJieHHyB KyjiBTypajr&Hyi) khäkoctl, a
1 υ. Ivi Is, ι : . . *· χ μ t/ \-
naTpyOOK 7 bhboäht OTpaöoTaHHHH ra3 no οτρβΛκβ C, κοτο-ρκπ Μοκβτ c5htb HOAaH Ha oqncTKy.
B yCTpofiCTBe jyiR COOpa H BHBOßa ΚΟΗΠβΗΤρΗρθΒ8ΗΗθδ OHOMaCCH npOHCXOflHT ΠΟΒΗΙϋθΗΠβ ΟΤβΠΘΗΗ KOHH.eHTpailJiH MHKpO-opraHH3MOB β πβΗΗοΙ φ83β, nocTynaiotqeH' η3 ΒβρχΗΗΧ 3 KOJEOHH I. IleHa nocTynaeT β KaMepy 12 ocyiHKH nemi,
TypÖyjieHTHoro nepeMememiH πθηη BBepx no Han-κ cÖopHHKy 10, προκοχο^ΗΤ HHTeHCHBHan ee ocymKa. B cyiy^iae ηθοοχοαημοοτη OKOH^aTejiLHoe raffleHHe neHH MoateT
ΠβΗ0Γ80ΗΤΘΛθΜ II. KOHIieHTpaT ÖHOMaCCH CO-
β cöopHHKe IO η οτβο,πητοη Hepe3 niTynep no cTpeji-κβ A.
KOHCTpyKTHBHOe ΒΗΠΟΛΗβΗΗβ ΚθΜβρΗ OCyiHKH ΠβΗΗ CIIOCOO-ycKopeHH» προτβκβΗΗΗ npoi^ecca oöe3BosHBaHHH neHH.
(|ia3a no ÄHHmy cTenaeT β BepxHioD cenmiK) 3 κολοηηη I η β BHse ocBeTJieHHofi xHÄKOCTH ^epe3 naTpyÖKH 6 nocTynaeT κ iiHpKyjifliiHOHHHM HacocaM 9.
KaiwepH ocyiüKH πθηη β Büße
H ΠρΗΚρβΠΛβΗΗΗΧ K ΗΘΗ ÖOJLbHlHMH OCHOBaHHfiMH
KOHycoB, oöpameHHHx μθηβιιιημη ocHOBaHHHMH BBepx η paenojiOKeHHHx oähh Has spyraiw c oöpa 30BaHHeM Meamy hh-3a3opa μη. npoxofla ocymeHHoö πθηη κ neHoracHTejoo ojia-BHÖopy HaKJioHa oopaeyicmet BepxHero KOHyca 45-60°, a HHsmero 20-45°, cnocoöcTByeT HanpaBJieHHOMy αβηαθηηκ neHH β 3aKpy^eHHOM (TaHreHiyiajiBHHH bboä) no BoexojyimeH cnnpaira ποτοκ c H3MeHiiE«iHMCJi jcaBjieHHeM Ha neHy 3a c^eT nepeMeHHoro ce^eHHH KaMepH, BCJieÄCTBHß qero aocTHraeTCa:
ΟΤβΠβΗΗ ΚΟΗΙίβΗΤρκρΟΒθΗΚΗ ÖHOMaCCH.
Pa3MemeHHHü BHyTpn KaMepH ocyiiiKH coocho eß cöophhk c ycTaHOBJieHHHM β BepxHeS ero nacTH neHoraodeene^HBaeT θφφβκτπΒΗοβ ocynieHHe neHH, nocje ee
Κ0ΗΙίβΗΤρΗρ0Β3ΗΗΗ B ΚθΜβρβ OCyHIKH H pa 3ΛβΛβΗΗβ CycneH3HH
Ha ra30ByBD φa3y η KOHueHTpHpoBaHHyro ÖHOMaccy, β ^ero Ha BHxoj[e ycTpotcTBa ÄOCTnraeTCH
ÖHOMaccH 60-80 γ/λ.
HcnojiB3OBaHHe ycTpoücTBa ,mm coopa H oTBOsa koh-ÖHOMaccH no3BOJweT ποβηοη-ϊβ cTeneHB koh
MHKp00praHH3M0B B ΠΘΗΘ H OTBOflHTB H3 aim
paTa ÖHOwiaccy c dojiBmefi KOHueHTpamaefi MHKpoopraHH3MOB, ^TO coKpamaeT eaTpaTH Ha CTasHH βη^θλθηηη MHKpoopraHH3
Hiaste npnBefleHH cpaBHHTeJiBHHe λβηηηθ no ÄpoaüKeBHX MHKpoopraHH3MOB β neHe, nojijnieHHHe npn HHH MOfleJieH yCTpoßCTB, ΒΗΠΟΛΗβΗΗΗΧ ΠΟ Η3ΒΘ0ΤΗ0Β^ H jiaraeMOMy
C ΠρΗΜβΗβΗΗβΜ HS- ΠΟ ΠρβΑΠαΓθβ-
BecTHoro ycTpoi- MOMy ycTpoii-CTBa CTBy
ÖHOMaccH 20,5 τ/Ji 20,0 γ/λ
ΚΟΗΙίβΗΤρΗρΟΒΘΗΗΗ 15 MHH 15 MHH
ÖHOMaecH 45 ν/π 70 τ/π
Jl;' I

Claims (1)

  1. ΦΟΒΙΗΜ
    I. AnnapaT &m BspaipiBaHHa MHKpoopraHH3MOB no aBTop-CBHfleTeJiLCTBy CCCP J£ 788726, OTJiH^aromnS-c π TeM, ^TO, c neJE&io nOBHmeima cTeneHH KOflqeflTpHpoBa-HHS OHOMaCCH, yCTpOÄCTBO AJM COOpa H BHBOSa ΚΟΗΙίβΗΤρίίρΟ- -
    öHOMaccH cflaoseHO KaMepot ocymKH nejHH, oopa3X3BaH-tcunimipH-qecKoa oOe^aäKot a πριικρθπλθηηημη κ Heii oojn>-
    OCHOBaHHHMH JiByMH yce^ieHHHMH KOHycaMH, OOpameHHHMH ΜβΗΜΠϊΜΗ OCHOBaHHHMH ΒΒβρΧ H paCHOJIOateHHHMH OÄHH Hafl ÄPy-ΓΗΜ C 3a3OpOIi MQXEJ HHMH JIM ΠρΟΧΟ^ OCyiDeHHOt ΠβΗΗ Κ Πβ-HoracHTejnD, πρπ θτομ yrojr HaiuioHa oöpasjOTieä Bepxnero KOHyca cocTaBJiseT 45-60°, a ηηχηθγο 20-45°.
    HCTO^HHKH HH$OpianHHf ΠρΗΗΗΤΗβ BO BHHMaHHe ΠρΗ 3Κ-
    cnepTH3e saHBKH*.
    I. ABTopcKoe CBHÄeTeJn>CTBO CCCP JS 788726, KJI.CI2B I/IO, 1979.
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AT336517B (de) * 1975-07-07 1977-05-10 Waagner Biro Ag Verfahren und einrichtung zur behandlung von flussigkeiten, wie z.b. truben mit einem fluid
SU653295A1 (ru) * 1977-04-15 1979-03-25 Предприятие П/Я Р-6729 Аппарат дл выращивани микроорганизмов
SU708696A1 (ru) * 1978-03-31 1986-11-15 Всесоюзный научно-исследовательский институт биосинтеза белковых веществ Аппарат дл выращивани микроорганизмов

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FR2503181B1 (de) 1984-05-04
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CS12582A1 (en) 1985-06-13
FR2503181A1 (fr) 1982-10-08

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