CY1125448T1 - Μεθοδοι για την ενισχυμενη αφαιρεση προσμειξεων κατα τη διαρκεια χρωματογραφιας πρωτεϊνης α - Google Patents

Μεθοδοι για την ενισχυμενη αφαιρεση προσμειξεων κατα τη διαρκεια χρωματογραφιας πρωτεϊνης α

Info

Publication number
CY1125448T1
CY1125448T1 CY20221100241T CY221100241T CY1125448T1 CY 1125448 T1 CY1125448 T1 CY 1125448T1 CY 20221100241 T CY20221100241 T CY 20221100241T CY 221100241 T CY221100241 T CY 221100241T CY 1125448 T1 CY1125448 T1 CY 1125448T1
Authority
CY
Cyprus
Prior art keywords
chromatography
methods
protein
during protein
impurities during
Prior art date
Application number
CY20221100241T
Other languages
English (en)
Inventor
Carl A. BEIGIE
Original Assignee
Genzyme Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genzyme Corporation filed Critical Genzyme Corporation
Publication of CY1125448T1 publication Critical patent/CY1125448T1/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G, L chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Water Supply & Treatment (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Στο παρόν έγγραφο παρέχονται μέθοδοι που σχετίζονται με τον καθαρμό ενός πολυπεπτιδίου που περιέχει μια περιοχή Fc (π.χ. ένα αντίσωμα) μέσω χρωματογραφίας πρωτεΐνης Α, μέθοδοι που σχετίζονται με τη χρήση ενός διαλύματος πλύσης που περιέχει βενζοϊκό άλας και/ή βενζυλική αλκοόλη κατά τη διάρκεια χρωματογραφίας πρωτεΐνης Α και μέθοδοι προσαρμογής μιας συλλογής με τη χρήση βενζοϊκού νατρίου προ της χρωματογραφίας πρωτεΐνης Α.
CY20221100241T 2017-12-21 2022-03-29 Μεθοδοι για την ενισχυμενη αφαιρεση προσμειξεων κατα τη διαρκεια χρωματογραφιας πρωτεϊνης α CY1125448T1 (el)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762609214P 2017-12-21 2017-12-21
US201862694387P 2018-07-05 2018-07-05
PCT/US2018/066890 WO2019126554A1 (en) 2017-12-21 2018-12-20 Methods for enhanced removal of impurities during protein a chromatography

Publications (1)

Publication Number Publication Date
CY1125448T1 true CY1125448T1 (el) 2024-02-16

Family

ID=65241305

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20221100241T CY1125448T1 (el) 2017-12-21 2022-03-29 Μεθοδοι για την ενισχυμενη αφαιρεση προσμειξεων κατα τη διαρκεια χρωματογραφιας πρωτεϊνης α

Country Status (18)

Country Link
US (2) US11236126B2 (el)
EP (2) EP4050016A1 (el)
JP (2) JP2021506925A (el)
KR (1) KR20200103740A (el)
CN (1) CN111712510B (el)
AU (1) AU2018392697A1 (el)
BR (1) BR112020012165A2 (el)
CA (1) CA3086186A1 (el)
CY (1) CY1125448T1 (el)
DK (1) DK3728288T3 (el)
ES (1) ES2907744T3 (el)
HU (1) HUE057850T2 (el)
IL (2) IL311002A (el)
MX (1) MX2020006586A (el)
SG (1) SG11202005809TA (el)
TW (2) TWI815838B (el)
WO (1) WO2019126554A1 (el)
ZA (1) ZA202003440B (el)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20220314142A1 (en) * 2019-07-01 2022-10-06 Pfizer Inc. Improvements to wash solutions for protein a chromatography in an antibody purification process
CN112827217A (zh) * 2020-12-29 2021-05-25 苏州良辰生物医药科技有限公司 一种层析柱清洗液及其应用
WO2024017826A2 (en) * 2022-07-19 2024-01-25 Glaxosmithkline Intellectual Property Development Limited Methods for purifying recombinant polypeptides
CN115894604B (zh) * 2022-12-16 2024-01-23 康日百奥生物科技(苏州)有限公司 重组蛋白澄清纯化方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4871667A (en) * 1984-11-26 1989-10-03 Agency Of Industrial Science & Technology Process for preparing muconic acid
US5750402A (en) * 1995-06-02 1998-05-12 Plant Cell Technology, Inc. Compositions and methods to prevent microbial contamination of plant tissue culture media
WO2006110277A1 (en) * 2005-04-11 2006-10-19 Medarex, Inc. Protein purification using hcic amd ion exchange chromatography
JP2006343214A (ja) * 2005-06-09 2006-12-21 Matsushita Electric Ind Co Ltd 免疫反応測定方法及びそれに用いる免疫反応測定用試薬
EP1994054A4 (en) 2006-01-06 2012-11-07 Amgen Inc METHODS AND SYSTEMS FOR ISOLATING TARGET MOLECULES OF COMPLEX COLUMN CHROMATOGRAPHY SOLUTIONS USING ELEMENTS CONTAINING ORGANIC SOLVENTS
SG10201702922VA (en) * 2008-10-20 2017-06-29 Abbvie Inc Isolation and purification of antibodies using protein a affinity chromatography
US10662237B2 (en) * 2009-08-06 2020-05-26 Genentech, Inc. Method to improve virus filtration capacity
DK2513134T3 (en) 2009-12-18 2017-12-18 Novartis Ag Washing solution and process for affinity chromatography
JP6023715B2 (ja) * 2010-10-11 2016-11-09 アッヴィ・バハマズ・リミテッド タンパク質の精製方法
CA2777978A1 (en) * 2011-05-27 2012-11-27 Abbott Biotherapeutics Corp. Dac hyp compositions and methods
KR102058067B1 (ko) * 2013-09-04 2019-12-20 이엠디 밀리포어 코포레이션 단백질 a 기반 친화성 크로마토그래피 컬럼의 세정 방법
US20170066839A1 (en) * 2015-09-08 2017-03-09 Merck Patent Gmbh Novel affinity chromatography media for removal of anti-a and/or anti-b antibodies

Also Published As

Publication number Publication date
JP2021506925A (ja) 2021-02-22
US11236126B2 (en) 2022-02-01
EP3728288B1 (en) 2022-01-19
US20220242902A1 (en) 2022-08-04
EP4050016A1 (en) 2022-08-31
EP3728288A1 (en) 2020-10-28
TW201936629A (zh) 2019-09-16
AU2018392697A1 (en) 2020-08-06
MX2020006586A (es) 2020-10-08
SG11202005809TA (en) 2020-07-29
JP2023145727A (ja) 2023-10-11
TWI815838B (zh) 2023-09-21
WO2019126554A1 (en) 2019-06-27
IL275418B2 (en) 2024-07-01
US20190233468A1 (en) 2019-08-01
DK3728288T3 (da) 2022-03-28
IL311002A (en) 2024-04-01
BR112020012165A2 (pt) 2020-11-24
ES2907744T3 (es) 2022-04-26
IL275418A (en) 2020-08-31
CA3086186A1 (en) 2019-06-27
TW202400623A (zh) 2024-01-01
RU2020123917A3 (el) 2022-01-26
CN111712510B (zh) 2024-06-14
CN111712510A (zh) 2020-09-25
HUE057850T2 (hu) 2022-06-28
KR20200103740A (ko) 2020-09-02
RU2020123917A (ru) 2022-01-26
IL275418B1 (en) 2024-03-01
ZA202003440B (en) 2021-10-27

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